| de novo assembly using low-coverage short read sequence data from the rice pathogen pseudomonas syringae pv. oryzae. | we developed a novel approach for de novo genome assembly using only sequence data from high-throughput short read sequencing technologies. by combining data generated from 454 life sciences (roche) and illumina (formerly known as solexa sequencing) sequencing platforms, we reliably assembled genomes into large scaffolds at a fraction of the traditional cost and without use of a reference sequence. we applied this method to two isolates of the phytopathogenic bacteria pseudomonas syringae. seque ... | 2009 | 19015323 |