| complete sequence of a picorna-like virus of the genus iflavirus replicating in the mite varroa destructor. | aggregations of 27 nm virus-like particles were observed in electron microscopy images of sectioned varroa destructor mite tissue. the scattered occurrence of individual particles and accumulation of the virions in lattices in the cytoplasm gave an apparent indication that the virus replicates in the mite. sequence analysis of the rna of the purified virus revealed a genome organization with high similarity to that of members of the genus iflavirus. phylogenetic analysis of the polymerase showed ... | 2004 | 15557248 |
| prevalence and phylogeny of kakugo virus, a novel insect picorna-like virus that infects the honeybee (apis mellifera l.), under various colony conditions. | we previously identified a novel insect picorna-like virus, termed kakugo virus (kv), from the brains of aggressive worker honeybees that had counterattacked a giant hornet. to survey the prevalence of kv in worker populations engaged in various labors, we quantified kv genomic rna. kv was detected specifically from aggressive workers in some colonies, while it was also detected from other worker populations in other colonies where the amount of kv detected in the workers was relatively high, su ... | 2006 | 16971448 |
| the 5' non-translated region of varroa destructor virus 1 (genus iflavirus): structure prediction and ires activity in lymantria dispar cells. | structure prediction of the 5' non-translated region (ntr) of four iflavirus rnas revealed two types of potential internal ribosome entry site (ires), which are discriminated by size and level of complexity, in this group of viruses. in contrast to the intergenic ires of dicistroviruses, the potential 5' ires structures of iflaviruses do not have pseudoknots. to test the activity of one of these, a bicistronic construct was made in which the 5' ntr of varroa destructor virus 1 (vdv-1) containing ... | 2006 | 17030876 |
| phylogenetic analysis of deformed wing virus genotypes from diverse geographic origins indicates recent global distribution of the virus. | honeybees originating from 10 different countries (austria, poland, germany, hungary, slovenia, nepal, sri lanka, the united arab emirates, canada, and new zealand) located on four continents were analyzed for the presence of deformed wing virus (dwv) nucleic acid by reverse transcription-pcr. two target regions within the dwv genome were selected for pcr amplification and subsequent sequencing, i.e., a region within the putative vp2 and vp4 structural-protein genes and a region within the rna h ... | 2007 | 17435003 |
| recombinants between deformed wing virus and varroa destructor virus-1 may prevail in varroa destructor-infested honeybee colonies. | we have used high-throughput illumina sequencing to identify novel recombinants between deformed wing virus (dwv) and varroa destructor virus-1 (vdv-1), which accumulate to higher levels than dwv in both honeybees and varroa destructor mites. the recombinants, vdv-1(vvd) and vdv-1(dvd), exhibit crossovers between the 5'-utr and the regions encoding the structural (capsid) and non-structural viral proteins. this implies that the genomes are modular and that each region may evolve independently, a ... | 2010 | 20926636 |
| [rt-pcr detection of deformed wing virus in the honey bee apis mellifera l. in the moscow region]. | deformed wing virus (dwv) was first detected in the honey bee apis mellifera by reverse transcriptase-polymerase chain reaction (rt-pct) in the moscow region. molecular phylogenetic analysis of the detected nucleotide sequence of the virus fragment vp2-vp1 of dwv demonstrated that the russian virus sequence is united in the common cluster with all earlier revealed nucleotide sequences of dwv in the genbank worldwide, which confirms the previous conclusions that this virus has recently distribute ... | 2010 | 21260995 |
| viruses associated with ovarian degeneration in apis mellifera l. queens. | queen fecundity is a critical issue for the health of honeybee (apis mellifera l.) colonies, as she is the only reproductive female in the colony and responsible for the constant renewal of the worker bee population. any factor affecting the queen's fecundity will stagnate colony development, increasing its susceptibility to opportunistic pathogens. we discovered a pathology affecting the ovaries, characterized by a yellow discoloration concentrated in the apex of the ovaries resulting from dege ... | 2011 | 21283547 |
| detection of honey bee (apis mellifera) viruses with an oligonucleotide microarray. | in recent years, declines in honey bee (apis mellifera l.) colonies have been observed to varying degrees worldwide with the worst losses in the usa being termed colony collapse disorder (ccd). pathogen load and the prevalence of honey bee viruses have been implicated in these losses and many diseased hives have multiple viruses present. we have designed and tested an oligonucleotide microarray which enables the simultaneous detection of nine honey bee viruses: acute bee paralysis virus, black q ... | 2011 | 21419132 |
| replication of varroa destructor virus 1 (vdv-1) and a varroa destructor virus 1-deformed wing virus recombinant (vdv-1-dwv) in the head of the honey bee. | a country-wide screen for viral pathogens in israeli apiaries revealed significant incidence of deformed wing virus (dwv) and varroa destructor-1 virus (vdv-1). to understand these viruses' possible involvement in deformed wing syndrome of honey bees, we studied their replication in symptomatically and asymptomatically infected bees qualitatively and quantitatively, using rt-pcr, quantitative real-time rt-pcr, and immunodetection of the major viral capsid protein vp1. we found, for the first tim ... | 2011 | 21652054 |
| priming effect of dengue and yellow fever vaccination on the immunogenicity, infectivity, and safety of a tetravalent dengue vaccine in humans. | a dengue vaccine effective against all four serotypes is urgently needed. however, safety and immunogenicity could be affected by prior exposure to flaviviruses. this open, controlled, phase iia study was conducted in 35 healthy adults who had received monovalent, live attenuated vero cell-derived dengue vaccine against dengue virus 1 (vdv1) or 2 (vdv2) or yellow fever (yf) vaccine 1 year before or who were flavivirus-naïve. all participants received one subcutaneous injection of tetravalent den ... | 2011 | 21976579 |
| two novel viruses associated with the apis mellifera pathogenic mite varroa destructor. | varroa destructor infestation of apis mellifera colonies carries and/or promotes replication of honey bee viruses like the deformed wing virus, the varroa destructor virus-1, the acute bee paralysis virus, the israeli acute bee paralysis virus and the kashmir bee virus that have been well described and characterized; but viruses exclusively associated with varroa were not found. to look for viruses that may associate with- or infect v. destructor we performed deep sequencing (rna-seq) of rna ext ... | 2016 | 27883042 |
| in-depth proteomic analysis of varroa destructor: detection of dwv-complex, abpv, vdmlv and honeybee proteins in the mite. | we investigated pathogens in the parasitic honeybee mite varroa destructor using nanolc-ms/ms (tripletof) and 2d-e-ms/ms proteomics approaches supplemented with affinity-chromatography to concentrate trace target proteins. peptides were detected from the currently uncharacterized varroa destructor macula-like virus (vdmlv), the deformed wing virus (dwv)-complex and the acute bee paralysis virus (abpv). peptide alignments revealed detection of complete structural dwv-complex block vp2-vp1-vp3, vd ... | 2015 | 26358842 |
| unbiased rna shotgun metagenomics in social and solitary wild bees detects associations with eukaryote parasites and new viruses. | the diversity of eukaryote organisms and viruses associated with wild bees remains poorly characterized in contrast to the well-documented pathosphere of the western honey bee, apis mellifera. using a deliberate rna shotgun metagenomic sequencing strategy in combination with a dedicated bioinformatics workflow, we identified the (micro-)organisms and viruses associated with two bumble bee hosts, bombus terrestris and bombus pascuorum, and two solitary bee hosts, osmia cornuta and andrena vaga. i ... | 2016 | 28006002 |
| seasonal prevalence of pathogens and parasites in the savannah honeybee (apis mellifera scutellata). | the loss of apis mellifera l. colonies in recent years has, in many regions of the world, been alarmingly high. no single cause has been identified for these losses, but the interactions between several factors (mostly pathogens and parasites) have been held responsible. work in the americas on honeybees originating mainly from south africa indicates that africanised honeybees are less affected by the interplay of pathogens and parasites. however, little is known about the health status of south ... | 2013 | 23702244 |
| evidence for positive selection and recombination hotspots in deformed wing virus (dwv). | deformed wing virus (dwv) is considered one of the most damaging pests in honey bees since the spread of its vector, varroa destructor. in this study, we sequenced the whole genomes of two virus isolates and studied the evolutionary forces that act on dwv genomes. the isolate from a varroa-tolerant bee colony was characterized by three recombination breakpoints between dwv and the closely related varroa destructor virus-1 (vdv-1), whereas the variant from the colony using conventional varroa man ... | 2017 | 28120868 |
| transcriptome analysis of the synganglion from the honey bee mite, varroa destructor and rnai knockdown of neural peptide targets. | varroa mites (varroa destructor) and the viruses that they transmit are one of the major contributing factors to the global honey bee crisis. gene products within the nervous system are the targets of all the insecticides currently used to control varroa but there is a paucity of transcriptomic data available for varroa neural tissues. a cdna library from the synganglia ("brains") of adult female varroa was constructed and 600 ests sequenced and analysed revealing several current and potential d ... | 2016 | 26721201 |
| mosaicsolver: a tool for determining recombinants of viral genomes from pileup data. | viral recombination is a key evolutionary mechanism, aiding escape from host immunity, contributing to changes in tropism and possibly assisting transmission across species barriers. the ability to determine whether recombination has occurred and to locate associated specific recombination junctions is thus of major importance in understanding emerging diseases and pathogenesis. this paper describes a method for determining recombinant mosaics (and their proportions) originating from two parent ... | 2014 | 25120266 |
| sequence recombination and conservation of varroa destructor virus-1 and deformed wing virus in field collected honey bees (apis mellifera). | we sequenced small (s) rnas from field collected honeybees (apis mellifera) and bumblebees (bombuspascuorum) using the illumina technology. the srna reads were assembled and resulting contigs were used to search for virus homologues in genbank. matches with varroadestructor virus-1 (vdv1) and deformed wing virus (dwv) genomic sequences were obtained for a. mellifera but not b. pascuorum. further analyses suggested that the prevalent virus population was composed of vdv-1 and a chimera of 5'-dwv- ... | 2013 | 24058580 |
| molecular characterization and phylogenetic analysis of deformed wing viruses isolated from south korea. | deformed wing virus (dwv) is one of the most common viral infection in honeybees. phylogenetic trees were constructed for 16 partial nucleotide sequences of the structural polyprotein region and the rna helicase region of south korean dwvs. the sequences were compared with 10 previously reported dwv sequences from different countries and the sequences of two closely related viruses, kakugo virus (kgv) and varroa destructor virus-1 (vdv-1). the phylogeny based on these two regions, the korean dwv ... | 2013 | 24035266 |