| use of an a-t-rich dna clone for identification and detection of peronosclerospora sorghi. | a recombinant plasmid, pmly12-1, screened from a peronosclerospora sorghi library hybridizes only to dna of p. sorghi, or to dna from leaves infected with p. sorghi, not to dna of p. sorghi thailand isolate, p. philippinensis, p. sacchari, or p. maydis. the terminal sequences of the 1.3-kb insert, which appears to contain mitochondrial dna, are 85% a and t. no polymorphisms were detected when the probe was hybridized to southern blots containing dna from p. sorghi pathotype 1, pathotype 3, or a ... | 1991 | 1892392 |
| cloning of aflp markers linked to resistance to peronosclerospora sorghi in maize. | genetic mapping of resistance genes for sorghum downy mildew (sdm) in maize revealed multiple-locus inheritance. a combination of aflp (amplified fragment length polymorphism) technique with bulked segregant analysis (bsa) was applied to map the genes involved in the resistance to sdm (peronosclerospora sorghi) in a recombinant inbred population. three aflp markers were identified and mapped to chromosomes 1 and 9, in regions previously associated with sdm resistance. one other aflp marker was f ... | 2002 | 12207229 |
| identification of qtls conferring resistance to downy mildews of maize in asia. | downy mildew is one of the most destructive diseases of maize in subtropical and tropical regions in asia. as a prerequisite for improving downy mildew resistance in maize, we analyzed quantitative trait loci (qtls) involved in resistance to the important downy mildew pathogens--peronosclerospora sorghi (sorghum downy mildew) and p. heteropogoni (rajasthan downy mildew) in india, p. maydis (java downy mildew) in indonesia, p. zeae in thailand and p. philippinensis in the philippines--using a rec ... | 2003 | 12759731 |
| identification and validation of qtls conferring resistance to sorghum downy mildew (peronosclerospora sorghi) and rajasthan downy mildew (p. heteropogoni) in maize. | we have mapped the quantitative trait loci (qtls) conferring resistance to sorghum downy mildew (peronosclerospora sorghi; sdm) and rajasthan downy mildew (p. heteropogoni; rdm), two species of dm prevalent throughout india. qtl mapping was carried out on a backcross population of 151 individuals derived from a cross between cm139 (susceptible parent) and nai116 (highly resistant to both sdm and rdm). heritability estimates were 0.74 for sdm and 0.67 for rdm. composite interval mapping combined ... | 2005 | 15841363 |
| characterization and genetic distance analysis of isolates of peronosclerospora sorghi using aflp fingerprinting. | sorghum downy mildew, caused by the obligate oomycete peronosclerospora sorghi, has been controlled through the use of resistant cultivars and seed treatment with metalaxyl. a recent outbreak in fields planted with treated seed revealed the presence of a metalaxyl-resistant variant. here, pcr-based methods including amplification from rapd primers and two systems of automated aflp analysis have been used to detect dna-level genetic variation among 14 isolates including metalaxyl-resistant and su ... | 2006 | 16546365 |
| simple sequence repeat markers useful for sorghum downy mildew (peronosclerospora sorghi) and related species. | a recent outbreak of sorghum downy mildew in texas has led to the discovery of both metalaxyl resistance and a new pathotype in the causal organism, peronosclerospora sorghi. these observations and the difficulty in resolving among phylogenetically related downy mildew pathogens dramatically point out the need for simply scored markers in order to differentiate among isolates and species, and to study the population structure within these obligate oomycetes. here we present the initial results f ... | 2008 | 19040756 |