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the impact of hybridization on long-term persistence of polyploid dactylorhiza species.hybridization may pose severe threats to the long-term survival of the parental taxa through introgression and the formation of hybrid swarms. however, when the resulting hybrids show strong male and female sterility, backcrossing and introgression are unlikely to occur, but the parental species may suffer from reduced male and female fitness.201627793859
desiccation tolerance, longevity and seed-siring ability of entomophilous pollen from uk native orchid species.pollinator-limited seed-set in some terrestrial orchids is compensated for by the presence of long-lived flowers. this study tests the hypothesis that pollen from these insect-pollinated orchids should be desiccation tolerant and relatively long lived using four closely related uk terrestrial species; anacamptis morio, dactylorhiza fuchsii, d. maculata and orchis mascula.201425006180
nuclear ribosomal dna sequence variation and evolution of spotted marsh-orchids (dactylorhiza maculata group).sequences of both internal and external transcribed spacers of nuclear ribosomal dna were sequenced for four species belonging to the dactylorhiza maculata group or "spotted marsh-orchids". these four species are d. fuchsii, d. saccifera, d. foliosa, and d. maculata. extensive nuclear ribosomal dna polymorphism was uncovered within the diploid d. fuchsii and the putative autotetraploid d. maculata. within the phylogenetic trees reconstructed using parsimony and bayesian analyses, four main linea ...200515939630
dactylorhiza (orchidaceae) in european russia: combined molecular and morphological analysis.four plastid and two nuclear (internal transcribed spacer [its] ribosomal dna) markers were used in this study of the dactylorhiza maculata and d. incarnata complexes (orchidaceae: orchidinae) to determine diversity and taxonomic distribution of haplotypes, hybridization frequencies, and maternal parentage of hybrids in 125 samples from 78 populations from european russia and the caucasus. a morphometric study of all populations revealed significant correspondence between morphological and plast ...200421652375
simultaneous preservation of orchid seed and its fungal symbiont using encapsulation-dehydration is dependent on moisture content and storage temperature.seeds of dactylorhiza fuchsii (common spotted orchid) and anacamptis morio (green-winged orchid) were encapsulated in alginate beads with hyphae of the basidomycete fungus ceratobasidium cornigerum. pre-treatment of beads for 18 h with sucrose at an optimum concentration of 0.75 m decreased the desiccation rate in a flow of sterile air (c. 23 degree c, 30% rh) and increased seed and fungal survival after up to 16 h drying. pre-treated and 16-h dried beads were transferred to cryo-vials and subse ...200212148057
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