| association between bifidobacteriaceae and the clinical severity of root caries lesions. | the isolation of members of the family bifidobacteriaceae (bifids) from oral samples has been sporadic and a recent cloning study has suggested that they are not detectable in root caries lesions. | 2009 | 19121067 |
| scardovia wiggsiae sp. nov., isolated from the human oral cavity and clinical material, and emended descriptions of the genus scardovia and scardovia inopinata. | six strains of anaerobic, pleomorphic gram-positive bacilli, isolated from the human oral cavity and an infected arm wound, were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group. 16s rrna gene sequence analysis revealed that the isolates were most closely related to scardovia inopinata ccug 35729(t) (94.8-94.9 % 16s rrna gene sequence similarity). the isolates were saccharolytic and produced acetic and lactic acids as end product ... | 2011 | 20139283 |
| cultivable anaerobic microbiota of severe early childhood caries. | severe early childhood caries (ecc), while strongly associated with streptococcus mutans using selective detection (culture, pcr), has also been associated with a widely diverse microbiota using molecular cloning approaches. the aim of this study was to evaluate the microbiota of severe ecc using anaerobic culture. the microbial composition of dental plaque from 42 severe ecc children was compared with that of 40 caries-free children. bacterial samples were cultured anaerobically on blood and ac ... | 2011 | 21289150 |
| microbiota of severe early childhood caries before and after therapy. | severe early childhood caries (ecc) is difficult to treat successfully. this study aimed to characterize the microbiota of severe ecc and evaluate whether baseline or follow-up microbiotas are associated with new lesions post-treatment. plaque samples from 2- to 6-year-old children were analyzed by a 16s rrna-based microarray and by pcr for selected taxa. severe-ecc children were monitored for 12 months post-therapy. by microarray, species associated with severe-ecc (n = 53) compared with caries ... | 2011 | 21868693 |
| anaerobic culture to detect periodontal and caries pathogens. | anaerobic culture has been critical in our understanding of the oral microbiotas. | 2014 | 25678835 |
| white-spot lesions and gingivitis microbiotas in orthodontic patients. | white-spot lesions (wsl) associated with orthodontic appliances are a cosmetic problem and increase risk for cavities. we characterized the microbiota of wsl, accounting for confounding due to gingivitis. participants were 60 children with fixed appliances, aged between 10 and 19 yrs, half with wsl. plaque samples were assayed by a 16s rrna-based microarray (homim) and by pcr. mean gingival index was positively associated with wsl (p = 0.018). taxa associated with wsl by microarray included gran ... | 2012 | 22837552 |
| analysis of bacterial activity in sound and cariogenic biofilm: a pilot in vivo study. | dental caries is a multifactorial disease with many associated microbial taxa, but only a few are notably contributing to acidogenicity. the ribosome number and the corresponding 16s ribosomal rna (rrna) concentration are considered a molecular indicator for general metabolic activity of bacteria, as they are elevated with increased anabolic and catabolic activities. we hypothesize that the activity of aciduric/acidogenic bacterial taxa, reflected by a rise in ribosomal counts, could resolve dif ... | 2016 | 27595541 |
| aciduric microbial taxa including scardovia wiggsiae and bifidobacterium spp. in caries and caries free subjects. | actinobacteria came into focus of being potential caries-associated pathogens and could, together with the established streptococcus mutans and lactobacilli thus function as caries indicator species. here we analyzed the role and diagnostic predictive value of the acidogenic-aciduric species scardovia wiggsiae and bifidobacterium dentium together with s. mutans, lactobacilli and bifidobacteria in biofilm of non-cavitated (n = 20) and cavitated (n = 6) caries lesions versus controls (n = 30). for ... | 2015 | 25933689 |
| screening of a pediatric and adult clinic population for caries pathogen scardovia wiggsiae. | dental caries remains one of the most common morbidities worldwide, with research focusing on the most prevalent cariogenic bacteria including streptococcus, actionmyces and lactobacillus species. however, recent evidence has begun to reveal novel pathogens that may alter current understanding of caries risk, including the recently discovered gram-positive bacillus scardovia wiggsiae. | 2017 | 27805882 |
| pcr-dipstick dna chromatography for profiling of a subgroup of caries-associated bacterial species in plaque from healthy coronal surfaces and periodontal pockets. | the onset of plaque-mediated disease, including dental caries and periodontal diseases, is highly associated with compositional change of the resident microflora from the ecological perspective. as specific bacterial profiles have been linked to different disease stages, microbial compositional measurements might therefore have great value for clinical diagnosis. previously we have reported a dry-reagent strip biosensor-pcr-dipstick dna chromatography, which utilized molecular recognition of oli ... | 2016 | 26912138 |
| rapid and sensitive pcr-dipstick dna chromatography for multiplex analysis of the oral microbiota. | a complex of species has been associated with dental caries under the ecological hypothesis. this study aimed to develop a rapid, sensitive pcr-dipstick dna chromatography assay that could be read by eye for multiplex and semiquantitative analysis of plaque bacteria. parallel oligonucleotides were immobilized on a dipstick strip for multiplex analysis of target dna sequences of the caries-associated bacteria, streptococcus mutans, streptococcus sobrinus, scardovia wiggsiae, actinomyces species, ... | 2014 | 25485279 |