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an homologous radioimmunoassay for coho salmon (oncorhynchus kisutch) vitellogenin, with general applicability to other pacific salmonids.this paper describes an homologous radioimmunoassay for coho salmon vitellogenin that demonstrates parallel cross-reactivity for plasma vitellogenin of all pacific salmonids tested (chinook, chum, coho, pink, and sockeye salmon, and cutthroat and rainbow trout), but not for atlantic salmon or two nonsalmonids: common carp and sablefish. plasma vitellogenin levels were high in ovulatory female pacific salmonids (micrograms/ml to mg/ml range), but were mostly nondetectable in spermiating males of ...19892767411
listeria monocytogenes contamination patterns for the smoked fish processing environment and for raw fish.reliable data on the sources of listeria monocytogenes contamination in cold-smoked fish processing are crucial in designing effective intervention strategies. environmental samples (n = 512) and raw fish samples (n = 315) from two smoked fish processing facilities were screened for l. monocytogenes, and all isolates were subtyped by automated ribotyping to examine the relationship between l. monocytogenes contamination from raw materials and that from environmental sites. samples were collected ...200312540181
health benefits and potential risks related to consumption of fish or fish oil.the nutritional benefits of fish consumption relate to the utilization of proteins of high biological value, as well as certain minerals and vitamins that fish provide. fish or fish oil contains omega-3 polyunsaturated fatty acids (pufas) that appear to play several useful roles for human health. conversely, some carcinogenic contaminants are also stored in the adipose tissue of fish. the objective of this paper is to evaluate the potential health benefits and risks related to the consumption of ...200314623484
identifying thresholds for ecosystem-based management.one of the greatest obstacles to moving ecosystem-based management (ebm) from concept to practice is the lack of a systematic approach to defining ecosystem-level decision criteria, or reference points that trigger management action.201020126647
description of five new loma (microsporidia) species in pacific fishes with redesignation of the type species loma morhua morrison & sprague, 1981, based on morphological and molecular species-boundaries tests.five new species of loma were described from five pacific fishes using light-microscopic and ultrastructural features along with phylogenetic analysis of the gene sequences of ribosomal rna (rrna) and elongation factor 1-alpha. morphological data revealed both qualitative and quantitative differences in developmental stages and timing, vesicles, xenoma features, and spore sizes with statistical support that differentiated loma pacificodae n. sp. in pacific cod, loma wallae n. sp. in walleye poll ...201021040100
residues of pbdes in northeastern pacific marine fish: evidence for spatial and temporal trends.in the flesh (skinless fillet) of chinook, chum, coho, pink, and sockeye salmon (oncorhynchus tshawytscha, o. keta, o. kisutch, o. gorbuscha, and o. nerka, respectively), sablefish (anoplopoma fimbria) and walleye pollock (theragra chalcogramma) from several sites in the northeast pacific sampled between 2002 and 2008, tetra- and pentabrominated diphenyl ethers (bdes) (bde 47, 49, 99, and 100) dominated the congener distribution. chinook and sablefish contained the highest concentrations, follow ...201121360729
permanent genetic resources added to molecular ecology resources database 1 august 2010-30 september 2010.this article documents the addition of 229 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: acacia auriculiformis × acacia mangium hybrid, alabama argillacea, anoplopoma fimbria, aplochiton zebra, brevicoryne brassicae, bruguiera gymnorhiza, bucorvus leadbeateri, delphacodes detecta, tumidagena minuta, dictyostelium giganteum, echinogammarus berilloni, epimedium sagittatum, fraxinus excelsior, labeo chrysophekadion, oncorhynch ...201121429127
global assessment of molecularly identified anisakis dujardin, 1845 (nematoda: anisakidae) in their teleost intermediate hosts.here, we present the its ribosomal dna (rdna) sequence data on 330 larvae of nematodes of the genus anisakis dujardin, 1845 collected from 26 different bony fish species from 21 sampling locations and different climatic zones. new host records are provided for anisakis simplex (rudolphi, 1809) sensu stricto (s.s.) and a. pegreffli campana-rouget et biocca, 1955 from anoplopoma fimbria (pallas) (santa barbara, east pacific), a. typica (diesing, 1860) from caesio cuning (bloch), lepturacanthus sav ...201323724731
two carboxypeptidase counterparts from rock bream (oplegnathus fasciatus): molecular characterization, genomic arrangement and immune responses upon pathogenic stresses.carboxypeptidases (cps) are proteases that hydrolyze c-terminal peptide bonds. they are involved in regulating the complement system of the immune system. here, we report the molecular characterization and immune response of two carboxypeptidases, named carboxypeptidase a (rb-cpa) and carboxypeptidase n1 (rb-cpn1), from rock bream. the genomic sequence of rb-cpa contains 12 exons interrupted by 11 introns, while the genomic sequence of rb-cpn1 has 9 exons and 8 introns. the cdna sequence of rb-c ...201425457548
a high-mobility group box 1 that binds to dna, enhances pro-inflammatory activity, and acts as an anti-infection molecule in black rockfish, sebastes schlegelii.high-mobility group box (hmgb) 1 is a chromosomal protein that plays critical roles in dna transcription, replication and repair. in addition, hmgb1 functions as a pro-inflammatory molecule in many vertebrates and invertebrates. in teleosts, very limited studies of hmgb1 have been reported. in this study, we identified a hmgb1 homologue (sshmgb1) from black rockfish (sebastes schlegelii) and analyzed its structure, expression and biological function. the open reading frame of sshmgb1 is 621 bp, ...201627492120
thioredoxin of golden pompano involved in the immune response to photobacterium damselae.thioredoxin (trx) is one of the key systems responsible for keeping the intracellular environment in a highly reduced state. in this study, a full-length trx cdna sequence (totrx) from golden pompano trachinotus ovatus was identified after pyrosequencing of golden pompano cdna library. totrx cdna is comprised of 786 bp, and contained a 324 bp open reading frame (orf) encoding a 107 amino acid polypeptide, a 5' untranslated region (utr) of 116 bp, and a long 3'- utr of 346 bp. multiple sequence a ...201526052015
phylogenetic analysis and positive-selection site detecting of vascular endothelial growth factor family in vertebrates.vascular endothelial growth factor (vegf), known to play an important role in vascular homeostasis, vascular integrity and angiogenesis, is little known about the evolutionary relationship of its five members especially the role of gene duplication and natural selection in the evolution of the vegf family. in this study, seventy-five full-length cdna sequences from 33 vertebrate species were extracted from the ncbi's genbank, uniprot protein database and the ensembl database. by phylogenetic ana ...201424200960
molecular cloning and expression analysis of small ubiquitin-like modifier (sumo) genes from grouper (epinephelus coioides).small ubiquitin-like modifier (sumo) is a group of proteins binding to lysine residues of target proteins and thereby modifying their stability, activity and subcellular localization. in the present study, two sumo homolog genes (ecsumo1 and ecsumo2) from grouper (epinephelus coioides) were cloned and characterized. the full-length sequence of ecsumo1 was 749 bp in length and contained a predicted open reading frame of 306 bp encoding 101 amino acids with a molecular mass of 11.34 kda. the full- ...201626616235
pathogenicity of members of the vibrionaceae family to cultured juvenile sablefish.sablefish anoplopoma fimbria are a prized seafood species due to their high oil content and white flaky flesh. raising these species in culture can help to provide an important source of protein for humans and relief to declining wild fish populations. understanding the environmental factors that influence the production of sablefish is important for successful culturing. the significance of host-pathogen interactions in sablefish culture and the resulting environmental implications are unknown. ...201525970236
high-frequency patterns in the abundance of benthic species near a cold-seep - an internet operated vehicle application.three benthic megafaunal species (i.e. sablefish anoplopoma fimbria; pacific hagfish eptatretus stoutii and a group of juvenile crabs) were tested for diel behavioral patterns at the methane hydrates site of barkley canyon (890 m depth), off vancouver island (bc, canada). fluctuations of animal counts in linear video-transects conducted with the internet operated deep-sea crawler "wally" in june, july and december of 2013, were used as proxy of population activity rhythms. count time series and ...201627732626
dimethylsulfoniopropionate (dmsp) increases survival of larval sablefish, anoplopoma fimbria.high concentrations of dimethylsulfoniopropionate (dmsp), a chemical compound released by lysed phytoplankton, may indicate high rates of grazing by zooplankton and may thus be a foraging cue for planktivorous fishes. previous studies have shown that some planktivorous fishes and birds aggregate or alter locomotory behavior in response to this chemical cue, which is likely adaptive because it helps them locate prey. these behavioral responses have been demonstrated in juveniles and adults, but n ...201627306913
a conventional multiplex pcr assay for the detection of toxic gemfish species (ruvettus pretiosus and lepidocybium flavobrunneum): a simple method to combat health frauds.the meat of ruvettus pretiosus and lepidocybium flavobrunneum (gemfishes) contains high amounts of indigestible wax esters that provoke gastrointestinal disorders. although some countries have banned the sale of these species, mislabeling cases have been reported in sushi catering. this work developed a simple conventional multiplex pcr, which discriminates the two toxic gemfishes from other potentially replaced species, such as tunas, cod, and sablefish. a common degenerate forward primer and t ...201626739756
modeling (137)cs bioaccumulation in the salmon-resident killer whale food web of the northeastern pacific following the fukushima nuclear accident.to track the long term bioaccumulation of (137)cs in marine organisms off the pacific northwest coast of canada, we developed a time dependent bioaccumulation model for (137)cs in a marine mammalian food web that included fish-eating resident killer whales. the model outcomes show that (137)cs can be expected to gradually bioaccumulate in the food web over time as demonstrated by the increase of the apparent trophic magnification factor of (137)cs, ranging from 0.76 after 1 month of exposure to ...201626657356
gonadal sex differentiation and effects of dietary methyltestosterone treatment in sablefish (anoplopoma fimbria).methods for sex control are needed to establish monosex aquaculture of sablefish (anoplopoma fimbria). here we conducted the first characterization of sex differentiation by histology and hormonal sex reversal experiment in sablefish. ovarian differentiation was first discernible at ~80 mm fork length (fl) and characterized by development of lamellar structures and onset of meiosis. testes exhibited a dual-lobe appearance over much of their length and remained non-meiotic until males were ≥520 m ...201626400269
molecular characterization of the gonadal kisspeptin system: cloning, tissue distribution, gene expression analysis and localization in sablefish (anoplopoma fimbria).the kisspeptin system plays pivotal roles in the regulation of vertebrate reproduction. classically, kisspeptin produced in the brain stimulates brain gonadotropin-releasing hormone signaling, which in turn activates the pituitary-gonad axis. expression of the kisspeptin system has also been documented in peripheral tissues, including gonads of mammals and fishes. however, the fish gonadal kisspeptin system remained uncharacterized. herein we report identification and characterization of four ki ...201626386183
genetic variability between complete mitochondrion genomes of the sablefish, anoplopoma fimbria (pallas, 1814).the complete mitochondrial genome of the sablefish, anoplopoma fimbria (genbank accession kp777542) is 16,507 bp in size and contains the typical 37 genes (13 protein-coding, 2 ribosomal rna, and 22 transfer rna) found in teleosts mitogenomes. the genome varies in 118 positions with respect to another mitogenome sablefish specimen.201626065847
development of approaches to induce puberty in cultured female sablefish (anoplopoma fimbria).efforts to establish sustainable and efficient aquaculture production of sablefish (anoplopoma fimbria) have been constrained by delayed puberty in cultured females. this study integrates a series of experiments aimed at gaining an understanding of the reproductive physiology of puberty in female sablefish. we detected transcripts for the dopamine d2 receptor (drd2) in brain, pituitary and ovary of sablefish, and prepubertal females exhibited significantly elevated brain and pituitary drd2 expre ...201525843684
micro-evolutionary processes in sablefish anoplopoma fimbria, based on polymorphism of the two sites of mitochondrial dna.sablefish anoplopoma fimbria is a deep-sea fish, endemic to the north pacific ocean, with continuous range from southern california to the central part of honshu island, including the bering and okhotsk seas. it is an important commercial species and a promising object for aquaculture. compared to the eastern part of the range the population structure of sablefish in asian waters is poorly studied. it is believed that sablefish goes to the bering sea and pacific waters of kamchatka and the kuril ...201425366282
analysis of sulfonamides, trimethoprim, fluoroquinolones, quinolones, triphenylmethane dyes and methyltestosterone in fish and shrimp using liquid chromatography-mass spectrometry.a liquid chromatography-tandem mass spectrometry (lc-ms/ms) screening method is described for the detection and identification of 26 veterinary drugs in fish and other aquaculture products. the analytes include: 13 sulfonamides, trimethoprim, 3 fluoroquinolones, 3 quinolones, 3 triphenylmethane dyes, 2 leuco dye metabolites, and 1 hormone. in this method, tissue is mixed with edta-mcilvaine buffer, double-extracted with acetonitrile, p-toluenesulfonic (p-tsa) acid and n,n,n',n'-tetramethyl-p-phe ...201425310706
a comparison of acoustic and visual metrics of sperm whale longline depredation.annual federal stock assessment surveys for alaskan sablefish also attempt to measure sperm whale depredation by quantifying visual evidence of depredation, including lip remains and damaged fish. a complementary passive acoustic method for quantifying depredation was investigated during the 2011 and 2012 survey hauls. a combination of machine-aided and human analysis counted the number of distinct “creak” sounds detected on autonomous recorders deployed during the survey, emphasizing sounds tha ...201424926504
killer whale depredation and associated costs to alaskan sablefish, pacific halibut and greenland turbot longliners.killer whale (orcinus orca) depredation (whales stealing or damaging fish caught on fishing gear) adversely impacts demersal longline fisheries for sablefish (anoplopoma fimbria), pacific halibut (hippoglossus stenolepis) and greenland turbot (reinhardtius hippoglossoides) in the bering sea, aleutian islands and western gulf of alaska. these interactions increase direct costs and opportunity costs associated with catching fish and reduce the profitability of longline fishing in western alaska. t ...201424558446
identification and characterization of rab7 from orange-spotted grouper, epinephelus coioides.rab7 is a small gtpase that regulates vesicular traffic from early to late endosomal stages of the endocytic pathway. during the virus-host co-evolution, host rab7 was also exploited by virus to complete their life cycle. to date, however, the roles of fish rab7 in virus infection remained largely unknown. here, we cloned and characterized a rab7 gene from grouper, epinephelus coioides (ec-rab7). the full-length ec-rab7 cdna was composed of 1182 bp and encoded a polypeptide of 207 amino acids wh ...201424161772
characterization of lps-induced tnfα factor (litaf) from orange-spotted grouper, epinephelus coioides.lipopolysaccharide-induced tnfα factor (litaf) is an important transcription factor that mediates cell apoptosis and inflammatory response. in the present study, we cloned and characterized a litaf gene from orange-spotted grouper (epinephelus coioides) (ec-litaf). ec-litaf encoded a predicted 142 amino acid protein which shared 74% identity to sablefish (anoplopoma fimbria) litaf homolog. multiple amino acid alignment showed that ec-litaf contained a typical litaf domain with two cxxc motifs. p ...201324091064
molecular characterization and quantification of sablefish (anoplopoma fimbria) gonadotropins and their receptors: reproductive dysfunction in female captive broodstock.efforts to establish an aquaculture industry for sablefish (anoplopoma fimbria) are constrained by reproductive dysfunction in wild-caught fish and by lack of reproduction of f1 females. toward a better understanding of the reproductive dysfunction of captive broodstock, full-length cdnas encoding the sablefish gonadotropin subunits (fshb, lhb and cga) and their receptors (fshr and lhcgr) were cloned, sequenced and quantitative real-time pcr assays developed. sablefish gonadotropin subunits disp ...201323892013
genomics of sablefish (anoplopoma fimbria): expressed genes, mitochondrial phylogeny, linkage map and identification of a putative sex gene.the sablefish (order: scorpaeniformes) is an economically important species in commercial fisheries of the north pacific and an emerging species in aquaculture. aside from a handful of sequences in ncbi and a few published microsatellite markers, little is known about the genetics of this species. the development of genetic tools, including polymorphic markers and a linkage map will allow for the successful development of future broodstock and mapping of phenotypes of interest. the significant s ...201323829495
molecular cloning, characterization, and sexually dimorphic expression of five major sex differentiation-related genes in a scorpaeniform fish, sablefish (anoplopoma fimbria).regardless of how sex is determined, the gonadal genes expressed downstream that regulate sex differentiation are relatively conserved among vertebrates. the goal of this study was to clone and characterize five key sex differentiation-related genes in a scorpaeniform fish, sablefish (anoplopoma fimbria). complete mrna sequences of foxl2, cyp19a1a, dmrt1, sox9a and amh were cloned, sequenced, and phylogenetically analyzed. the sablefish mrna sequences exhibited the characteristic domains of each ...201323507626
acoustic and diving behavior of sperm whales (physeter macrocephalus) during natural and depredation foraging in the gulf of alaska.sperm whales have depredated black cod (anoplopoma fimbria) from demersal longlines in the gulf of alaska for decades, but the behavior has recently spread in intensity and geographic coverage. over a three-year period 11 bioacoustic tags were attached to adult sperm whales off southeast alaska during both natural and depredation foraging conditions. measurements of the animals' dive profiles and their acoustic behavior under both behavioral modes were examined for statistically significant diff ...201222779498
a risk-benefit analysis of wild fish consumption for various species in alaska reveals shortcomings in data and monitoring needs.northern peoples face a difficult decision of whether or not to consume wild fish, which may contain dangerous levels of contaminants such as methylmercury (mehg), but which also offer a number of positive health benefits, and play an important role in rural household economies. here, new methods for developing consumption advice are applied to an existing data-set for methylmercury (mehg) levels in alaskan fish. we apply a quantitative risk-benefit analysis for eight freshwater, saltwater and a ...201020673961
use it or lose it? sablefish, anoplopoma fimbria, a species representing a fifth teleostean group where the betanhe associated with the red blood cell adrenergic stress response has been secondarily lost.like most teleosts, sablefish (anoplopoma fimbria pallas 1814) blood exhibits a moderate root effect (~35% maximal desaturation), where a reduction in blood ph dramatically reduces o(2) carrying capacity, a mechanism important for oxygenating the eye and filling the swim bladder (sb) in teleosts. although sablefish lack a sb, we observed a well-defined choroid rete at the eye. the adrenergically mediated cell swelling typically associated with a functional red blood cell (rbc) beta-adrenergic na ...201020400635
relationship between sperm whale (physeter macrocephalus) click structure and size derived from videocamera images of a depredating whale (sperm whale prey acquisition).sperm whales have learned to depredate black cod (anoplopoma fimbria) from longline deployments in the gulf of alaska. on may 31, 2006, simultaneous acoustic and visual recordings were made of a depredation attempt by a sperm whale at 108 m depth. because the whale was oriented perpendicularly to the camera as it contacted the longline at a known distance from the camera, the distance from the nose to the hinge of the jaw could be estimated. allometric relationships obtained from whaling data an ...200919425683
transition from open access to quota based fishery management regimes in alaska increased the safety of operations.during the past 12 years fishery managers responsible for federal fisheries off alaska have developed and implemented three new fishery management limited access/quota share programs in place of traditional open access management. the three limited access programs are unique but each provides for the allocation of quota shares to individual participants in the halibut and sablefish longline fishery, in the bering sea pollock trawl fishery and in the bering sea king and tanner crab fishery, respe ...200718350974
thermal modulation of the toxicokinetics of benzo[a]pyrene in isolated hepatocytes of sablefish (anoplopoma fimbria), black rockfish (sebastes melanops), and chub mackerel (scomber japonicus).hepatocytes from sablefish (anoplopoma fimbria), black rockfish (sebastes melanops) and chub mackerel (scomber japonicus) were isolated from 11 degrees c acclimated animals. the uptake, metabolism, and excretion of benzo[a]pyrene (b[a]p) in hepatocytes was measured at 6, 11 and 19 degrees c. chub mackerel hepatocyte uptake rates were significantly lower (0.012 +/- 0.003 microg/s per g cell) at 11 degrees c than black rockfish (0.028 +/- 0.009 microg/s per g cell) or sablefish (0.032 +/- 0.012 mi ...199910622431
radionuclides in fishes and mussels from the farallon islands nuclear waste dump site, california.the farallon islands nuclear waste dump site (finwds), approximately 30 miles west of san francisco, california, received at least 500 tbq encapsulated in more than 47,500 containers from approximately 1945 to 1970. during several seasons in 1986/87 deep-sea bottom feeding fishes (dover sole = microstomus pacificus; sablefish = anoplopoma fimbria; thornyheads = sebastolobus spp.) and intertidal mussels (mytilus californianus) were collected from the vicinity of the finwds and from comparable dep ...19968690599
comparison of three species of dietary fish: effects on serum concentrations of low-density-lipoprotein cholesterol and apolipoprotein in normotriglyceridemic subjects.limited information is available comparing the effect of different species of fish on serum low-density-lipoprotein-cholesterol (ldl-c) and apolipoprotein b (apo b) concentrations. we fed 21 normotriglyceridemic males diets containing different species of fish (200 g dover sole, chinook salmon, or sablefish) for 18 d in a three-period crossover design. concentrations of apo b and ldl-c rose on the salmon and sablefish diets compared with the sole diet (p = 0.02 for apo b, 0.08 for ldl-c). these ...19911907090
comparison of three species of fish consumed as part of a western diet: effects on platelet fatty acids and function, hemostasis, and production of thromboxane.this study examined the effect of the consumption of moderate amounts of three species of fish on platelet fatty acid profile (pfa), bleeding time (bt), platelet aggregation, and ex vivo production of thromboxane b2 (txb2). twenty-three healthy, young men consumed 200 g/d of dover sole. chinook salmon, and sablefish for 18 d in a three-way crossover design as part of a western diet. salmon and sablefish compared with sole caused significant increases in the eicosapentaenoic acid content of the p ...19911858697
short communication: induced ovulation of sablefish (anoplopoma fimbria) following oral administration of des gly(10)-(d-ala (6))lh-rh ethylamide. 199024221036
the genus leptotheca (protozoa: myxosporida) in macrourid fishes and sablefish, anoplopoma fimbria.five species of leptotheca, one new, were found in the macrourid fishes and the sablefish, anoplopoma fimbria. those species which were geographically widespread in numerous host species showed little variation. two leptotheca species were found both in the gall bladder and urinary bladder/kidney.19761003339
ceratomyxa anoplopoma sp.n. (protozoa, myxosporida) from the sablefish, anoplopoma fimbria. 1976963646
the myrosperidan genus davisia in two deep-sea fishes.two new species of davisia were identified in the urinary bladders and kidney tubules of 2 species of marine fish. d. anoplopoma was found in anoplopoma fimbria from southern and central california. d. pectoralis was recorded from coryphaenoides pectoralis from northern california. we have distinguished between davisia species whose lateral appendages are "solid" and those which contain a cavity in their lateral appendages. our species belong to the latter category. we believe the lateral append ...1975809565
n-nitrosodimethylamine in cold-smoked sablefish. 20131141510
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