| a study of weeds as potential inoculum sources for a tomato-infecting begomovirus in central brazil. | tomato severe rugose virus (tosrv) is the most important begomovirus species in brazilian tomato production. many weeds are associated with tomato, and some are hosts of begomoviruses. only one species of weed, nicandra physaloides, has been found to be infected with tosrv. in this study, four weed species were investigated for their capacity to be infected by tosrv and serve as a potential source of inoculum for tomato. begomoviruses from naturally infected crotalaria spp., euphorbia heterophyl ... | 2013 | 23489523 |
| in planta cloning of geminiviral dna: the true sida micrantha mosaic virus. | the circular single-stranded dnas of geminiviruses are multiplied efficiently and preferentially by rolling circle amplification (rca), and can be diagnosed readily by restriction fragment length polymorphism (rflp) and direct sequencing of the rca product. two strategies are described for cloning geminiviruses from plants harboring mixed infections by using rca and rflp with plant-derived nucleic acids without the need for bacterial amplification. by combining both these approaches, the true si ... | 2010 | 19878698 |
| the genomes of four novel begomoviruses and a new sida micrantha mosaic virus strain from bolivian weeds. | begomovirus is the largest genus within the family geminiviridae and includes economically important plant dna viruses infecting a broad range of plant species and causing devastating crop diseases, mainly in subtropical and tropical countries. besides cultivated plants, many weeds act as virus reservoirs. eight begomovirus isolates from bolivian weeds were examined using rolling-circle amplification (rca) and restriction fragment length polymorphism (rflp). an efficient, novel cloning strategy ... | 2011 | 21170729 |
| three distinct begomoviruses associated with soybean in central brazil. | we report the complete nucleotide sequences of geminiviruses of the genus begomovirus infecting soybean (glycine max) in central brazil. samples obtained from soybean plants collected at santo antonio de goiás, goiás state, showing typical symptoms of viral infection, were analyzed. infection was confirmed by pcr-based amplification of a dna-a fragment with universal begomovirus primers. total dna from infected plants was then subjected to rolling-circle amplification (rca), and 2.6-kb molecules ... | 2009 | 19636495 |