| inhibition of virus dna replication by artificial zinc finger proteins. | prevention of virus infections is a major objective in agriculture and human health. one attractive approach to the prevention is inhibition of virus replication. to demonstrate this concept in vivo, an artificial zinc finger protein (azp) targeting the replication origin of the beet severe curly top virus (bsctv), a model dna virus, was created. in vitro dna binding assays indicated that the azp efficiently blocked binding of the viral replication protein (rep), which initiates virus replicatio ... | 2005 | 15681461 |
| transcriptional activator elements for curtovirus c1 expression reside in the 3' coding region of orf c1. | beet curly top virus (bctv) and beet severe curly top virus (bsctv), members of curtoviruses, encode seven open reading frames (orfs) within a approximately 3 kb genome. one of these viral orfs, c1, is known to play an important role in the early stage of viral infection in plants during initiation of viral dna replication. we used promoter:: reporter (beta-glucuronidase) gene fusions in transgenic ara-bidopsis to identify the putative promoter region of bctv orf c1. unlike other geminiviruses, ... | 2007 | 17464215 |
| inhibition of tomato yellow leaf curl virus replication by artificial zinc-finger proteins. | previously, we designed an artificial zinc-finger protein (azp) for blocking a replication protein (rep) of beet severe curly top virus (bsctv) from binding to its replication origin and demonstrated that transgenic arabidopsis plants expressing the azp are completely resistant to the virus infection. here we applied the azp technology to tomato yellow leaf curl virus (tylcv) infective to an important agricultural crop, tomato. we designed an azp binding to the direct repeat to block the tylcv r ... | 2007 | 18029770 |
| identification of a promoter motif involved in curtovirus sense-gene expression in transgenic arabidopsis. | expression of the seven open reading frames (orfs) of single-stranded dna curtoviruses such as beet curly top virus (bctv) and beet severe curly top virus (bsctv) is driven by a bi-directional promoter. to investigate this bi-directional promoter activity with respect to viral late gene expression, transgenic arabidopsis plants expressing a gus reporter gene under the control of either the bctv or bsctv bi-directional promoter were constructed. transgenic plants harboring constructs showed highe ... | 2008 | 18596416 |
| generation of plants resistant to tomato yellow leaf curl virus by using artificial zinc-finger proteins. | previously, we designed an artificial zinc-finger protein (azp) for blocking a replication protein (rep) of beet severe curly top virus (bsctv) from binding to its replication origin and demonstrated that transgenic arabidopsis plants expressing the azp are completely resistant to the virus infection. here we applied the azp technology to tomato yellow leaf curl virus (tylcv) infective to an important agricultural crop, tomato. we designed and constructed an azp binding to the direct repeat to b ... | 2008 | 18776317 |
| curly top survey in the western united states. | curly top in sugar beet continues to be a challenging disease to control in the western united states. to aid in development of host resistance and management options, the curtovirus species composition was investigated by sampling 246 commercial fields along with nursery and field trials in the western united states. dna was isolated from leaf samples and the species were identified using species-specific polymerase chain reaction primers for the c1 gene. amplicons from 79 isolates were also se ... | 2008 | 18943410 |
| spinach curly top virus: a newly described curtovirus species from southwest texas with incongruent gene phylogenies. | abstract a curtovirus associated with a disease of spinach was isolated in southwest texas during 1996. disease symptoms included severe stunting and chlorosis, with younger leaves curled, distorted, and dwarfed. viral dna was purified and an infectious clone obtained. agroinoculation using a construct bearing full-length tandem repeats of the cloned viral genome resulted in systemic infection of species in six of seven plant families tested, indicating that the virus has a wide host range. symp ... | 2004 | 18943911 |
| replication specificity elements of the worland strain of beet curly top virus are compatible with those of the cfh strain but not those of the cal/logan strain. | abstract cloned genomes of the cfh, worland, and cal/logan strains of beet curly top virus (bctv) served as helper viruses to trans-replicate defective (d) dnas that are incapable of self-replication due to deletions within the c1 open reading frame encoding the replication initiator (rep) protein. the logan rep protein could trans-replicate a logan-derived d dna in a transient replication assay conducted in nicotiana benthamiana leaf disks. however, the logan rep protein was unable to trans-rep ... | 1998 | 18944850 |
| rkp, a ring finger e3 ligase induced by bsctv c4 protein, affects geminivirus infection by regulation of the plant cell cycle. | the c4 protein from curtovirus is known as a major symptom determinant, but the mode of action of the c4 protein remains unclear. to understand the mechanism of involvement of c4 protein in virus-plant interactions, we introduced the c4 gene from beet severe curly top virus (bsctv) into arabidopsis under a conditional expression promoter; the resulting overexpression of bsctv c4 led to abnormal host cell division. rkp, a ring finger protein, which is a homolog of the human cell cycle regulator k ... | 2009 | 19000158 |
| curtovirus-cucurbit interaction: acquisition host plays a role in leafhopper transmission in a host-dependent manner. | curly top disease (ctd) of vegetable crops is caused by viruses in the genus curtovirus (family geminiviridae). cucurbits are reported to be susceptible to ctd; however, the disease is rare in california despite annual outbreaks in other hosts (e.g., common bean, pepper, sugar beet, and tomato). consistent with these observations, no obvious curly top symptoms were observed in melon fields surveyed for ctd in central california in 2004 and 2005, whereas the disease was readily observed in tomato ... | 2009 | 19055441 |
| characterization of a new curtovirus, pepper yellow dwarf virus, from chile pepper and distribution in weed hosts in new mexico. | over 4,950 asymptomatic weed samples from more than 20 weed species that are host plants for curtoviruses were collected from ten chile pepper fields in southern new mexico (nm) during 2003, 2004 and 2005 to identify whether they were infected with curtoviruses and to determine which curtoviruses were distributed in the weed population. polymerase chain reaction using primers designed to detect a portion of the coat protein (cp) gene were used to detect curtoviruses, and infected plants were fur ... | 2009 | 19198754 |
| up-regulation of lsb1/gdu3 affects geminivirus infection by activating the salicylic acid pathway. | geminiviruses include a large number of single-stranded dna viruses that are emerging as useful tools to dissect many fundamental processes in plant hosts. however, there have been no reports yet regarding the genetic dissection of the geminivirus-plant interaction. here, a high-throughput approach was developed to screen arabidopsis activation-tagged mutants which are resistant to geminivirus beet severe curly top virus (bsctv) infection. a mutant, lsb1 (less susceptible to bsctv 1), was identi ... | 2010 | 20042021 |
| involvement of c4 protein of beet severe curly top virus (family geminiviridae) in virus movement. | beet severe curly top virus (bsctv) is a leafhopper transmitted geminivirus with a monopartite genome. c4 proteins encoded by geminivirus play an important role in virus/plant interaction. | 2010 | 20585583 |
| c4 protein of beet severe curly top virus is a pathomorphogenetic factor in arabidopsis. | the curtovirus c4 protein is required for symptom development during infection of arabidopsis. transgenic arabidopsis plants expressing c4 from either beet curly top virus or beet severe curly top virus produced phenotypes that were similar to symptoms seen during infection with wild-type viruses. the pseudosymptoms caused by c4 protein alone were novel to transgenic arabidopsis and included bumpy trichomes, severe enations, disorientation of vascular bundles and stomata, swelling, callus-like s ... | 2010 | 20960205 |
| bsctv c2 attenuates the degradation of samdc1 to suppress dna methylation-mediated gene silencing in arabidopsis. | plant viruses are excellent tools for studying microbial-plant interactions as well as the complexities of host activities. our study focuses on the role of c2 encoded by beet severe curly top virus (bsctv) in the virus-plant interaction. using bsctv c2 as bait in a yeast two-hybrid screen, a c2-interacting protein, s-adenosyl-methionine decarboxylase 1 (samdc1), was identified from an arabidopsis thaliana cdna library. the interaction was confirmed by an in vitro pull-down assay and a firefly l ... | 2011 | 21245466 |
| the arabidopsis thaliana homeobox gene athb12 is involved in symptom development caused by geminivirus infection. | geminiviruses are single-stranded dna viruses that infect a number of monocotyledonous and dicotyledonous plants. arabidopsis is susceptible to infection with the curtovirus, beet severe curly top virus (bsctv). infection of arabidopsis with bsctv causes severe symptoms characterized by stunting, leaf curling, and the development of abnormal inflorescence and root structures. bsctv-induced symptom development requires the virus-encoded c4 protein which is thought to interact with specific plant- ... | 2011 | 21625602 |
| next-generation sequencing and genome editing in plant virology. | next-generation sequencing (ngs) has been applied to plant virology since 2009. ngs provides highly efficient, rapid, low cost dna, or rna high-throughput sequencing of the genomes of plant viruses and viroids and of the specific small rnas generated during the infection process. these small rnas, which cover frequently the whole genome of the infectious agent, are 21-24 nt long and are known as vsrnas for viruses and vd-srnas for viroids. ngs has been used in a number of studies in plant virolo ... | 2016 | 27617007 |
| arabidopsis thaliana remorins interact with snrk1 and play a role in susceptibility to beet curly top virus and beet severe curly top virus. | remorins, a family of plant-specific proteins containing a variable n-terminal region and conserved c-terminal domain, play a role in various biotic and abiotic stresses, including host-microbe interactions. however, their functions remain to be completely elucidated, especially for the arabidopsis thaliana remorin group 4 (atrem4). to elucidate the role of remorins in arabidopsis, we first showed that atrem4s have typical molecular characteristics of the remorins, such as induction by various t ... | 2014 | 25289013 |
| establishing a crispr-cas-like immune system conferring dna virus resistance in plants. | crispr-cas (clustered, regularly interspaced short palindromic repeats-crispr-associated proteins) is an adaptive immune system in many archaea and bacteria that cleaves foreign dna on the basis of sequence complementarity. here, using the geminivirus, beet severe curly top virus (bsctv), transient assays performed in nicotiana benthamiana demonstrate that the sgrna-cas9 constructs inhibit virus accumulation and introduce mutations at the target sequences. further, transgenic arabidopsis and n. ... | 2015 | 27251395 |
| altered invertase activities of symptomatic tissues on beet severe curly top virus (bsctv) infected arabidopsis thaliana. | arabidopsis thaliana infected with beet severe curly top virus (bsctv) exhibits systemic symptoms such as stunting of plant growth, callus induction on shoot tips, and curling of leaves and shoot tips. the regulation of sucrose metabolism is essential for obtaining the energy required for viral replication and the development of symptoms in bsctv-infected a. thaliana. we evaluated the changed transcript level and enzyme activity of invertases in the inflorescence stems of bsctv-infected a. thali ... | 2013 | 23589148 |
| c2-mediated decrease in dna methylation, accumulation of sirnas, and increase in expression for genes involved in defense pathways in plants infected with beet severe curly top virus. | cytosine methylation is one of epigenetic information marked on the dna sequence. in plants, small interfering rnas (sirnas) target homologous genomic dna sequences for cytosine methylation. this process, known as rna-directed dna methylation (rddm), plays an important role in transposon control, regulation of gene expression and virus resistance. in this paper, we demonstrate that the c2 protein encoded by a geminivirus (beet severe curly top virus, bsctv) mediated a decrease in dna methylation ... | 2013 | 23176533 |
| cotton leaf curl multan betasatellite as a plant gene delivery vector trans-activated by taxonomically diverse geminiviruses. | cotton leaf curl multan betasatellite (clcumb) replicates in tobacco, tomato and datura plants in the presence of the helper viruses tomato leaf curl virus-australia, iranian isolates of tomato yellow leaf curl virus, tomato leaf curl karnataka virus, and beet severe curly top virus (bsctv). infectious recombinant clcumb constructs were made in which segments of either the camv 35s or the petunia chsa promoter replaced the clcumb βc1 orf, and these were designated pbinβδc1-35s and pbinβδc1-chsa, ... | 2012 | 22476203 |