| identification of regions of the beet mild curly top virus (family geminiviridae) capsid protein involved in systemic infection, virion formation and leafhopper transmission. | plant viruses in the genus curtovirus (family geminiviridae) are vectored by the beet leafhopper (circulifer tenellus) and cause curly top disease in a wide range of dicotyledonous plants. an infectious clone of an isolate of beet mild curly top virus (bmctv-[w4]), associated with an outbreak of curly top in pepper and tomato crops, was characterized and used to investigate the role of the capsid protein (cp) in viral biology and pathogenesis. frameshift mutations were introduced into the overla ... | 2005 | 16085227 |
| curly top survey in the western united states. | curly top in sugar beet continues to be a challenging disease to control in the western united states. to aid in development of host resistance and management options, the curtovirus species composition was investigated by sampling 246 commercial fields along with nursery and field trials in the western united states. dna was isolated from leaf samples and the species were identified using species-specific polymerase chain reaction primers for the c1 gene. amplicons from 79 isolates were also se ... | 2008 | 18943410 |
| spinach curly top virus: a newly described curtovirus species from southwest texas with incongruent gene phylogenies. | abstract a curtovirus associated with a disease of spinach was isolated in southwest texas during 1996. disease symptoms included severe stunting and chlorosis, with younger leaves curled, distorted, and dwarfed. viral dna was purified and an infectious clone obtained. agroinoculation using a construct bearing full-length tandem repeats of the cloned viral genome resulted in systemic infection of species in six of seven plant families tested, indicating that the virus has a wide host range. symp ... | 2004 | 18943911 |
| distribution and rate of movement of the curtovirus beet mild curly top virus (family geminiviridae) in the beet leafhopper. | abstract a polymerase chain reaction (pcr)-based method for the detection of the curtovirus beet mild curly top virus (bmctv, previously named the worland strain of beet curly top virus) was developed and used to investigate the bmctv-beet leafhopper interaction. using pcr and a bmctv-specific primer pair, an approximately 1.1-kb bmctv dna fragment was amplified from adult leafhoppers and from the organs involved in circulative transmission: the digestive tract, hemolymph, and salivary glands. t ... | 2003 | 18944363 |
| replication specificity elements of the worland strain of beet curly top virus are compatible with those of the cfh strain but not those of the cal/logan strain. | abstract cloned genomes of the cfh, worland, and cal/logan strains of beet curly top virus (bctv) served as helper viruses to trans-replicate defective (d) dnas that are incapable of self-replication due to deletions within the c1 open reading frame encoding the replication initiator (rep) protein. the logan rep protein could trans-replicate a logan-derived d dna in a transient replication assay conducted in nicotiana benthamiana leaf disks. however, the logan rep protein was unable to trans-rep ... | 1998 | 18944850 |
| curtovirus-cucurbit interaction: acquisition host plays a role in leafhopper transmission in a host-dependent manner. | curly top disease (ctd) of vegetable crops is caused by viruses in the genus curtovirus (family geminiviridae). cucurbits are reported to be susceptible to ctd; however, the disease is rare in california despite annual outbreaks in other hosts (e.g., common bean, pepper, sugar beet, and tomato). consistent with these observations, no obvious curly top symptoms were observed in melon fields surveyed for ctd in central california in 2004 and 2005, whereas the disease was readily observed in tomato ... | 2009 | 19055441 |
| characterization of a new curtovirus, pepper yellow dwarf virus, from chile pepper and distribution in weed hosts in new mexico. | over 4,950 asymptomatic weed samples from more than 20 weed species that are host plants for curtoviruses were collected from ten chile pepper fields in southern new mexico (nm) during 2003, 2004 and 2005 to identify whether they were infected with curtoviruses and to determine which curtoviruses were distributed in the weed population. polymerase chain reaction using primers designed to detect a portion of the coat protein (cp) gene were used to detect curtoviruses, and infected plants were fur ... | 2009 | 19198754 |
| turnip curly top virus, a highly divergent geminivirus infecting turnip in iran. | from 2006 onwards turnip crops in fars province, iran, have been noted with unusual leaf curling and vein swelling symptoms which are characteristic of the leafhopper-transmitted viruses of the genus curtovirus (family geminiviridae). rolling circle amplification was used to clone viruses from five turnip isolates exhibiting leaf curl symptoms. analysis of the sequences showed them to have >93% sequence identity and to be distinct from all other geminiviruses previously characterised. analysis o ... | 2010 | 20566344 |
| genetic diversity in curtoviruses: a highly divergent strain of beet mild curly top virus associated with an outbreak of curly top disease in pepper in mexico. | a full-length curtovirus genome was pcr-amplified and cloned from peppers in mexico with symptoms of curly top disease. the cloned dna of this isolate, mx-p24, replicated in nicotiana tabacum protoplasts and was infectious in n. benthamiana plants. sequence analysis revealed that the mx-p24 isolate had a typical curtovirus genome organization and was most similar to beet mild curly top virus (bmctv). however, sequence identities were at the threshold value for establishment of a new curtovirus s ... | 2010 | 21193937 |