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stability of microbial communities in goat milk during a lactation year: molecular approaches.the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ...200717604934
[diversity of culturable bacteria associated with the sea urchin hemicentrotus pulcherrimus from naozhou island].to investigate the diversity of culturable bacteria isolated from the sea urchin hemicentrotus pulcherrimus collected from a tidal flat of naozhou island (20 degrees 52'n - 20 degrees 56'n 110 degrees 33'e - 110 degrees 38'e), leizhou bay, south china sea, china.200920112668
notes on the almost unknown genus jeotgalicoccus.to facilitate isolation and differentiation of the almost entirely unknown jeotgalicoccus spp.201020156307
terminal olefin (1-alkene) biosynthesis by a novel p450 fatty acid decarboxylase from jeotgalicoccus species.terminal olefins (1-alkenes) are natural products that have important industrial applications as both fuels and chemicals. however, their biosynthesis has been largely unexplored. we describe a group of bacteria, jeotgalicoccus spp., which synthesize terminal olefins, in particular 18-methyl-1-nonadecene and 17-methyl-1-nonadecene. these olefins are derived from intermediates of fatty acid biosynthesis, and the key enzyme in jeotgalicoccus sp. atcc 8456 is a terminal olefin-forming fatty acid de ...201121216900
jeotgalicoccus huakuii sp. nov., a halotolerant bacterium isolated from seaside soil.a gram-stain-positive, oxidase- and catalase-positive, non-motile, non-spore-forming, halotolerant, coccoid bacterium, designated strain ny-2(t), was isolated from a seaside soil sample from shandong province, china. strain ny-2(t) was able to grow in the presence of 0-23 % (w/v) nacl and at ph 4.5-10.0 and 5-42 degrees c; optimum growth was observed with 3-8 % (w/v) total salts and at ph 6.5-8.0 and 28-37 degrees c. chemotaxonomic analyses, including fatty acid profiles, menaquinones and polar ...201019667366
jeotgalicoccus marinus sp. nov., a marine bacterium isolated from a sea urchin.a novel non-sporulating, non-motile, catalase- and oxidase-positive, facultatively anaerobic, moderately halophilic, gram-positive coccus, designated jsm 076033(t), was isolated from a sea urchin (hemicentrotus pulcherrimus) collected from the south china sea. strain jsm 076033(t) was able to grow in the presence of 0.5-25.0 % (w/v) total salts and at ph 6.0-10.0 and 10-45 degrees c; optimum growth was observed with 5.0-10.0 % (w/v) total salts and at ph 7.0-8.0 and 25-30 degrees c. the major am ...200919542134
nosocomiicoccus ampullae gen. nov., sp. nov., isolated from the surface of bottles of saline solution used in wound cleansing.strains trf-1(t) and tc-9 were isolated from transfer spikes of two separate bottles containing 0.9 % nacl (physiological saline) solution used repeatedly to wash wounds in hospital wards, months apart. phylogenetic analysis of 16s rrna gene sequences revealed that strains trf-1(t) and tc-9 formed a distinct branch within the family bacillaceae most closely related to the members of the genus jeotgalicoccus. the two strains, with identical 16s rrna gene sequences, showed sequence similarities of ...200819060087
changes in bacterial communities accompanied by aggregation in a fed-batch composting reactor.the contents of fed-batch composting (fbc) reactors often aggregate after prolonged operation. this process leads to irreversible breakdown of the decomposition reaction and possible alteration of the bacterial communities. we compared the structures of bacterial communities in reactors under aggregate and optimal conditions. the results of 16s rrna gene clone analysis showed that populations of the family bacillaceae (such as bacillus spp., cerasibacillus spp., gracilibacillus spp.), which domi ...200818231830
salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional korean fermented seafood.a novel, moderately halophilic, gram-positive coccus, designated strain s2r53-5(t), was isolated from jeotgal, a traditional korean fermented seafood. the organism was strictly aerobic, non-motile, non-sporulating and catalase- and oxidase-positive. strain s2r53-5(t) grew in the presence of 0.5-15 % (w/v) nacl and at ph 6.5-11.0, with optimum growth at 5 % (w/v) nacl and ph 7.0. the temperature range for growth was 20.0-30.0 degrees c, with an optimum temperature of 30 degrees c. phylogenetic an ...200717329798
microbial community structure and dynamics of dark fire-cured tobacco fermentation.the italian toscano cigar production includes a fermentation step that starts when dark fire-cured tobacco leaves are moistened and mixed with ca. 20% prefermented tobacco to form a 500-kg bulk. the dynamics of the process, lasting ca. 18 days, has never been investigated in detail, and limited information is available on microbiota involved. here we show that toscano fermentation is invariably associated with the following: (i) an increase in temperature, ph, and total microbial population; (ii ...200717142368
jeotgalicoccus pinnipedialis sp. nov., from a southern elephant seal (mirounga leonina).a previously unknown gram-positive, catalase-positive, facultatively anaerobic, non-spore-forming, coccus-shaped bacterium (a/g14/99/10(t)), originating from the mouth of a female southern elephant seal, was subjected to a taxonomic analysis. comparative 16s rrna gene-sequencing showed that the organism formed a hitherto unknown subline within the catalase-positive, low-g+c, gram-positive cocci, exhibiting a specific association with species of the genus jeotgalicoccus. sequence divergence value ...200415143018
jeotgalicoccus halotolerans gen. nov., sp. nov. and jeotgalicoccus psychrophilus sp. nov., isolated from the traditional korean fermented seafood jeotgal.two gram-positive, non-motile, non-spore-forming, halotolerant and moderately halophilic cocci (strains ykj-101t and ykj-115t) were isolated from the traditional korean fermented seafood jeotgal, and were investigated using a polyphasic taxonomic approach. phylogenetic analysis of 16s rdna sequences showed that strains ykj-101t and ykj-115t are most closely related to the cluster comprising two salinicoccus species. the peptidoglycan type of the strains is a3alpha, based on l-lys-gly(3-4)-l-ala( ...200312710632
molecular diversity of bacteria in yunnan yellow cattle (bos taurs) from nujiang region, china.the rumen content of four yunnan yellow cattle (bos taurs) were collected to determine the bacteria diversity by using 16s rrna gene sequence analysis. a total of 129 sequences were examined and the sequences were referred as 107 otu (operational taxonomy unit) according to the similarity level of 97% in gene sequence. similarity analysis revealed that yunnan yellow cattle had 12 sequences (10 otu) shared 97% or greater similarity with cultured rumen bacteria butyrivibrio fibrisolvens, succinicl ...201121598111
jeotgalicoccus nanhaiensis sp. nov., isolated from intertidal sediment of the south china sea, and emended description of the genus jeotgalicoccus.a novel non-sporulating, non-motile, catalase- and oxidase-positive, strictly aerobic, gram-positive coccus, strain jsm 077023t, was isolated from an intertidal sediment sample collected from naozhou island in the south china sea, china. growth occurred in the presence of 0.5-25 % (w/v) nacl [optimum, 2-5 % (w/v) nacl] and at ph 5.5-10.5 (optimum, ph 7.0-8.0) and at 4-45 °c (optimum, 30-35 °c). the major amino acid constituents of the cell wall were alanine, glycine and lysine. the major cellula ...201020851914
jeotgalicoccus halophilus sp. nov., isolated from salt lakes in xinjiang, china.two moderately halophilic bacterial strains c1-52t and yd-9, isolated from daban and aiding salt lakes in xinjiang, china, respectively, were identified using a polyphasic approach. the strains were gram-positive, non-endospore-forming, non-motile, facultatively anaerobic and coccus-shaped bacteria. colonies were pale yellow in colour and light pink diffusible pigment was produced after a few additional days incubation. the two strains grew optimally in the presence of 2-3 % (w/v) nacl, ph 7.5 a ...201020802063
detection of jeotgalicoccus spp. in poultry house air.investigations of bioaerosols collected from turkey, chicken and duck houses, as well as from a duck slaughterhouse, each in triplicate, revealed that 4-18% of 16s rrna gene sequences in investigated 16s rrna gene clone libraries were closely related to jeotgalicoccus spp. j. halotolerans- and j. psychrophilus-related sequences were obtained in all investigated bioaerosol samples and formed a distinct group with sequences of both species type strains, which were collectively entitled jeot-cluste ...201020435421
jeotgalicoccus coquinae sp. nov. and jeotgalicoccus aerolatus sp. nov., isolated from poultry houses.two gram-stain-positive, non-motile, non-spore-forming cocci (strains mk-7(t) and mpa-33(t)) were isolated from poultry houses. strain mk-7(t) was isolated on marine broth agar from coquina, a food supplement for female ducks used in a duck-fattening farm. strain mpa-33(t) was isolated from the air of a turkey house on tsa after filter sampling. on the basis of 16s rrna gene sequence similarity studies, both strains were shown to belong to the genus jeotgalicoccus; mk-7(t) was most closely relat ...201120207804
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