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pannonibacter phragmitetus gen. nov., sp. nov., a novel alkalitolerant bacterium isolated from decomposing reed rhizomes in a hungarian soda lake.three alkalitolerant bacterial strains were isolated from the surface of decomposing rhizomes of reed [phragmites australia (cav.) trin. et steudel] in lake fertö (hungary). cells of the novel isolates were gram-negative, motile rods and formed star-shaped aggregates. they were facultatively anaerobic and chemo-organotrophic. bacteriochlorophyll a was not synthesized under aerobic conditions. the strains were catalase and oxidase positive, produced acid from d-glucose under aerobic and anaerobic ...200312710626
diversity of alkaliphilic and alkalitolerant bacteria cultivated from decomposing reed rhizomes in a hungarian soda lake.bacterial communities associated with decomposing rhizomes of phragmites australis were investigated in lake ferto (neusiedlersee, hungary). alkaliphilic and alkalitolerant strains were isolated on cellulose-containing alkaline medium spread with dilutions of scrapings taken from the surface of the decaying plant material. fifty-one strains were grouped by numerical analysis based on physiological tests and biolog sole carbon source utilization data. the strains identified by 16s rdna sequence c ...200516132425
stappia alba sp. nov., isolated from mediterranean oysters.three bacterial strains isolated from oysters recovered at the spanish mediterranean coast have been phenotypically and genetically characterized. the results of the phylogenetic analysis based on almost complete 16s rdna sequences clustered all three strains together with 99.9% average sequence similarity and situated them in the neighbourhood of the genera stappia, roseibium and pannonibacter, stappia aggregata being their closest neighbour with sequence similarities between 98.8% and 98.9%. d ...200516261856
physiological, ecological, and phylogenetic characterization of stappia, a marine co-oxidizing bacterial genus.bacteria play a major role in marine co cycling, yet very little is known about the microbes involved. thirteen co-oxidizing stappia isolates obtained from existing cultures, macroalgae, or surf samples representing geographically and ecologically diverse habitats were characterized using biochemical, physiological, and phylogenetic approaches. all isolates were aerobic chemoorganotrophs that oxidized co at elevated (1,000 ppm) and ambient-to-subambient concentrations (<0.3 ppm). all contained t ...200717142374
pannonibacter phragmitetus, described from a hungarian soda lake in 2003, had been recognized several decades earlier from human blood cultures as achromobacter groups b and e.we performed a polyphasic taxonomic study on isolates previously tentatively classified as achromobacter groups b and e in comparison with the type strain of pannonibacter phragmitetus, lmg 22736(t)=nctc 13350(t). comparative 16s rrna gene sequence analysis suggested that strains of achromobacter groups b and e belong to p. phragmitetus (similarity levels were higher than 99 %). dna-dna hybridization experiments and other genotypic and phenotypic analyses confirmed that the three taxa represent ...200617159003
description of labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of stappia aggregata as labrenzia aggregata comb. nov., of stappia marina as labrenzia marina comb. nov. and of stappia alba as labrenzia alba comb. nov., and emended descriptions of the genera pannonibacter, stappia and roseibium, and of the species roseibium denhamense and roseibium hamelinense.a slightly pink-coloured strain, strain dfl-11(t), was isolated from single cells of the marine dinoflagellate alexandrium lusitanicum and was found to contain the genes encoding two proteins of the photosynthetic reaction centre, pufl and pufm. 16s rrna gene sequence analysis revealed that the novel strain belonged to the alpha-2 subgroup of the proteobacteria and was most closely related to stappia aggregata (97.7 % similarity), stappia alba (98.0 %) and stappia marina (98.0 %). dark-grown cel ...200717473266
aerobic biodegradation of tert-butyl alcohol (tba) by psychro- and thermo-tolerant cultures derived from granular activated carbon (gac).tert-butyl alcohol (tba) is a metabolite of methyl tert-butyl ether and is itself possibly a fuel oxygenate. the goals of this study were to enrich and characterize tba-degrading micro-organism(s) from a granular activated carbon (gac) unit currently treating tba. the results reported herein describe the first aerobic, tba-degrading cultures derived from gac. strains kr1 and yz1 were enriched from a gac sample in a bicarbonate-buffered freshwater medium. tba was degraded to 10% of the initial co ...200817541707
cr (vi) remediation by indigenous bacteria in soils contaminated by chromium-containing slag.hexavalent chromium (cr) is a toxic element causing serious environmental threat. recently, more and more attention is paid to the bio-remediation of cr (vi) in the contaminated soils. cr (vi) remediation by indigenous bacteria in soils contaminated by chromium-containing slag at a steel-alloy factory in hunan province, china, was investigated in the present study. the results showed that when sufficient nutrients were amended into the contaminated soils, total cr (vi) concentration declined fro ...200919246154
hexavalent chromium reduction by pannonibacter phragmitetus bb isolated from soil under chromium-containing slag heap.the objective of this study was to find an indigenous cr(vi)-reducing bacterium that can effectively be used for cr(vi) remediation in the contaminated soils. the results showed that one isolate from soil under a chromium-containing slag heap at a steel-alloy factory in china had a strong ability of reducing cr(vi). it can completely reduce 500 mg l(-1) cr (vi) within 24 h. based on 16s rrna gene sequence and similarity analysis, this isolate was identified as pannonibacter phragmitetus and assi ...200919337925
biodegradation of 4-aminobenzenesulfonate by a novel pannonibacter sp. w1 isolated from activated sludge.4-aminobenzenesulfonate (4-abs) is a representative intermediate of some sulfonated azo dyes. a novel pannonibacter sp. strain w1 capable of degrading 4-abs as sole carbon as well as energy, nitrogen and sulfur source was isolated and identified from the activated sludge of a municipal wastewater treatment plant. strain w1 was able to completely degrade 4-abs with initial concentrations of 200-2500 mg l(-1) within 40 h. haldane inhibition model was used to fit the special degradation rate at dif ...200919423220
[isolation and identification of a 4-aminobenzenesulphonate biodegrading strain and its degradation characteristics].a 4-aminobenzenesulphonate-degrading strain w1 was isolated from the activated sludge of dalian municipal wastewater treatment plant. strain w1 was able to utilize 4-abs as sole carbon and energy source under aerobic condition. it was identified as pannonibacter sp. according to its morphological, physiological and biochemical characteristics and the analysis of its 16s rdna gene. the optimum conditions for 4-abs biodegradation in the shaking flasks were 10% inoculum, 30 degrees c, ph 7.0 and we ...200919545028
[kinetics and mechanism of 4-aminobenzenesulphonate biodegradation].kinetics of 4-aminobensenesulphonate (4-abs) degradation by pannonibacter sp. w1 under aerobic condition was studied. at medium and low initial concentration (50-1000 mg/l), the 4-abs could be degraded almost completely in 14 h,and the degradation reaction conformed to one order kinetics. at high initial concentration (1 200-2500 mg/l), up to 90% degradation rate could be achieved in 32 h, and it conformed to zero order kinetics in earlier period of degradation and one order kinetics in later pe ...200919775021
agaricicola taiwanensis gen. nov., sp. nov., an alphaproteobacterium isolated from the edible mushroom agaricus blazei.a gram-negative, beige-pigmented, aerobic, motile, club-shaped bacterium, designated strain cc-sbabm117(t), was isolated from the stipe of the edible mushroom agaricus blazei murrill. 16s rrna gene sequence analysis demonstrated that the strain shared <93 % similarity with the type strains of species in the genera pannonibacter, methylopila, nesiotobacter and stappia. the organism was unable to produce acid from carbohydrates, but utilized a number of organic acids and amino acids. ubiquinone 10 ...201019819997
reduction of hexavalent chromium by pannonibacter phragmitetus lsse-09 stimulated with external electron donors under alkaline conditions.a novel cr (vi) resistant bacterial strain lsse-09, identified as pannonibacter phragmitetus, was isolated from industrial sludge. it has strong aerobic and anaerobic cr (vi)-reduction potential under alkaline conditions. at 37 °c and ph 9.0, growing cells of strain lsse-09 could completely reduce 100 and 1000 mg l(-1) cr (vi)-cr (iii) within 9 and 24h, respectively under aerobic condition. resting cells showed higher anaerobic reduction potential with the rate of 1.46 mg g(-1)((dry weight))min( ...201021041020
genetic and phenotypic diversity of plant growth promoting rhizobacteria isolated from sugarcane plants growing in pakistan.bacteria were isolated from roots of sugarcane varieties grown in the fields of punjab. they were identified by using api20e/ne bacterial identification kits and from sequences of 16s rrna and amplicons of the cpn60 gene. the majority of bacteria were found to belong to the genera of enterobacter, pseudomonas, and klebsiella, but members of genera azospirillum, rhizobium, rahnella, delftia, caulobacter, pannonibacter, xanthomonas, and stenotrophomonas were also found. the community, however, was ...201021193815
reduction of hexavalent chromium by pannonibacter phragmitetus lsse-09 coated with polyethylenimine-functionalized magnetic nanoparticles under alkaline conditions.a novel cell separation and immobilization method for cr (vi)-reduction under alkaline conditions was developed by using superparamagnetic fe(3)o(4) nanoparticles (nps). the fe(3)o(4) nps were synthesized by coprecipitation followed by modification with sodium citrate and polyethyleneimine (pei). the surface-modified nps were monodispersed and the particle size was about 15 nm with a saturation magnetization of 62.3 emu/g and an isoelectric point (pi) of 11.5 at room temperature. pei-modified fe ...201121440990
encapsulation of pannonibacter phragmitetus lsse-09 in alginate-carboxymethyl cellulose capsules for reduction of hexavalent chromium under alkaline conditions.cr(vi) was efficiently reduced to cr(iii) by pannonibacter phragmitetus lsse-09 encapsulated in liquid-core alginate-carboxymethyl cellulose capsules under alkaline conditions. taking into account the physical properties of the capsules, the activity of encapsulated cells, and total cr(iii) concentration in the supernatant, optimal conditions (0.5% w/v sodium alginate; 2% w/v sodium carboxymethyl cellulose; 0.1 m cacl(2); 30-min gelation time) for lsse-09 encapsulation were determined. at optima ...201121442414
rubrimonas shengliensis sp. nov. and polymorphum gilvum gen. nov., sp. nov., novel members of alphaproteobacteria from crude oil contaminated saline soil.four bacterial strains were isolated from a crude oil contaminated saline soil in shengli oilfield, china. strains sl014b-28a2(t) and sl014b-80a1 were most closely related to rubrimonas cliftonensis och 317(t), while strains sl003b-26a1(t) and sl003b-26a2 were most closely related to but readily different from the species in the pannonibacter-labrenzia-roseibium-stappia cluster. the major fatty acids were c(18:1)ω7c, c(16:0), c(18:0) and 11-methyl c(18:1)ω7c, and c(18:1)ω7c, 11-methyl c(18:1)ω7c ...201121600718
enhanced biodegradation of 4-aminobenzenesulfonate in membrane bioreactor by pannonibacter sp. w1.4-aminobenzenesulfonate (4-abs), an aromatic amine and recalcitrant toxic pollutant, is widely used in the dye and pharmaceutical industry. pannonibactersp. w1 is a specialized microbial strain which can efficiently degrade 4-abs. this study shows the feasibility of using the specialized strain in an mbr system to treat synthetic wastewater containing large amount of 4-abs. due to membrane retention, the biomass concentration is able to reach 5 g/l within two months of continuous operation. pann ...201122049775
identification and hexavalent chromium reduction characteristics of pannonibacter phragmitetus.a hexavalent chromium [cr(vi)] reducing bacterial strain was isolated from chromium-containing slag. it was identified as pannonibacter phragmitetus based on physiological, biochemical characteristics and 16s rrna gene sequence analysis. this bacterium displayed great cr(vi) reduction capability. the cr(vi) could be completely removed in 24 h under anaerobic condition when the initial concentration was 1,917 mg l(-1), with the maximum reduction rate of 562.8 mg l(-1) h(-1). the cr(vi) reduction ...201122179413
in vitro reduction of hexavalent chromium by cytoplasmic fractions of pannonibacter phragmitetus lsse-09 under aerobic and anaerobic conditions.hexavalent chromate reductase was characterized and was found to be localized in the cytoplasmic fraction of a chromium-resistant bacterium pannonibacter phragmitetus lsse-09. the cr(vi) reductase activity of cell-free extract (s(12)) was significantly improved by external electron donors, such as nadh, glucose, acetate, formate, citrate, pyruvate, and lactate. the reductase activity was optimal at ph 7.0 with nadh as the electron donor. the aerobic and anaerobic cr(vi)-reduction enhanced by 0.1 ...201122161214
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