| characterization of a new 1,3-1,4-β-glucanase gene from bacillus tequilensis cgx5-1. | 1,3-1,4-β-glucanase received great interest due to its application in brewing and feed industries. application of 1,3-1,4-β-glucanase in brewing industry helps make up for the defect that plant-derived β-glucanases are heat-sensitive. a new strain, cgx5-1, exhibited remarkable 1,3-1,4-β-glucanase, was isolated from asian giant hornet nest and identified bacillus tequilensis. moreover, a new 1,3-1,4-β-glucanase gene from b. tequilensis was cloned and measured to be 720 bp encoding 239 amino acids ... | 2014 | 24728764 |
| acidogenic spent wash valorization through polyhydroxyalkanoate (pha) synthesis coupled with fermentative biohydrogen production. | the production of polyhydroxyalkanoates (phas) by bacillus tequilensis biocatalyst using spent wash effluents as substrate was evaluated to increase the versatility of the existing pha production process and reduce production cost. in this study, spent wash was used as a substrate for biohydrogen (h2) production and the resulting acidogenic effluents were subsequently employed as substrate for pha production. maximum h2 production of 39.8l and maximum pha accumulation of 40% dry cell weight was ... | 2014 | 24637295 |
| cytotoxic effect of microbial biosurfactants against human embryonic kidney cancerous cell: hek-293 and their possible role in apoptosis. | two different microbial biosurfactants s9bs and chbs were isolated from lysinibacillus fusiformis s9 and bacillus tequilensis ch. cytotoxicity effect of these biosurfactants on human embryonic kidney cancerous cell (hek-293) were studied with the help of 3-(4,5-dimethylthiazol-2yl-)-2, 5-diphenyl tetrazolium bromide (mtt) assay and morphological changes were observed under inverted microscope. the biosurfactants exhibited positive cytotoxic effect on hek-293 cell line. it was found that lc50 of ... | 2014 | 25149458 |
| nitrogen acquisition in agave tequilana from degradation of endophytic bacteria. | plants form symbiotic associations with endophytic bacteria within tissues of leaves, stems, and roots. it is unclear whether or how plants obtain nitrogen from these endophytic bacteria. here we present evidence showing nitrogen flow from endophytic bacteria to plants in a process that appears to involve oxidative degradation of bacteria. in our experiments we employed agave tequilana and its seed-transmitted endophyte bacillus tequilensis to elucidate organic nitrogen transfer from (15)n-label ... | 2014 | 25374146 |
| enhanced production and characterization of a solvent stable amylase from solvent tolerant bacillus tequilensis rg-01: thermostable and surfactant resistant. | ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. among them, culture rg-01 was adjudged as the best amylase producer and was identified as bacillus tequilensis from mtcc, chandigarh. the isolate showed maximum amylase production (8100 u/ml) in the presence of starch, peptone, and ca(2+) ions at 55°c ph 7.0 within 24 h of incubation. the enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n ... | 2014 | 25401163 |
| an extracellular thermo-alkali-stable laccase from bacillus tequilensis sn4, with a potential to biobleach softwood pulp. | degradation of residual lignin in kraft pulp by chemical bleaching is implicated in causing environmental pollution. the use of thermo- and alkali-tolerant bacterial laccases is considered to be important biological alternative to chemical processing. laccases from bacillus species have shown promise in this respect but their intracellular/spore bound presence make their industrial application economically unfeasible. we report here on a novel extracellular active thermo-alkali-stable laccase (s ... | 2015 | 28324575 |
| a novel keratinase from bacillus tequilensis strain q7 with promising potential for the leather bating process. | the present paper reports on the purification and characterization of an extracellular keratinase (kerq7) newly purified from bacillus tequilensis q7. pure protein was obtained after ammonium sulfate fractionation (30-60%), followed by mono s sepharose cation-exchange chromatography. maldi-tof/ms analysis revealed that the purified enzyme was a monomer with a molecular mass of 28,355.07-da. the sequence of the 21 n-terminal residues of kerq7 showed high homology with those of bacillus keratinase ... | 2015 | 26086793 |
| comparative assessment of the apoptotic potential of silver nanoparticles synthesized by bacillus tequilensis and calocybe indica in mda-mb-231 human breast cancer cells: targeting p53 for anticancer therapy. | recently, the use of nanotechnology has been expanding very rapidly in diverse areas of research, such as consumer products, energy, materials, and medicine. this is especially true in the area of nanomedicine, due to physicochemical properties, such as mechanical, chemical, magnetic, optical, and electrical properties, compared with bulk materials. the first goal of this study was to produce silver nanoparticles (agnps) using two different biological resources as reducing agents, bacillus tequi ... | 2015 | 26170659 |
| draft genome sequence of bacillus tequilensis strain fjat-14262a. | bacillus tequilensis fjat-14262a is a gram-positive rod-shaped bacterium. here, we report the 4,038,551-bp genome sequence of b. tequilensis fjat-14262a, which will provide useful information for genomic taxonomy and phylogenomics of bacillus. | 2015 | 26564040 |
| isolation and identification of a novel fibrinolytic bacillus tequilensis cwd-67 from dumping soils enriched with poultry wastes. | a newly isolated strain, cwd-67, which exhibited high fibrinolytic activity, was screened from dumping soils enriched with poultry wastes. the strain was identified as bacillus tequilensis (kf897935) by 16sr rna gene sequence analysis and biochemical characterization. a fibrinolytic enzyme was purified to homogeneity from the culture supernatant using ammonium sulfate precipitation, membrane concentration, dialysis, ion-exchange, and gel filtration chromatography. sds-page analysis showed that t ... | 2015 | 26782654 |
| novel serine keratinase from caldicoprobacter algeriensis exhibiting outstanding hide dehairing abilities. | the current paper reports on the purification of an extracellular thermostable keratinase (kerca) produced from caldicoprobacter algeriensis strain th7c1(t), a thermophilic, anaerobic bacterium isolated from a hydrothermal hot spring in algeria. the maximum keratinase activity recorded after 24-h of incubation at 50 °c was 21000 u/ml. the enzyme was purified by ammonium sulfate precipitation-dialysis and heat treatment (2h at 50 °c) followed by uno q-6 fplc anion exchange chromatography, and sub ... | 2016 | 26812107 |
| retting and degumming of natural fibers by pectinolytic enzymes produced from bacillus tequilensis sv11-uv37 using solid state fermentation. | the present study demonstrated the simultaneous production and optimization of pectinolytic enzymes (pectate lyase and polygalacturonase) under ssf from bacillus tequilensis sv11-uv37 using wheat bran as a substrate, which is commercially viable and cost-effective. optimization by one variable-at-a-time-approach showed a maximum yield of pectate lyase (1371.25 u/gds) and polygalacturonase (85.45 u/gds) with wheat bran using 80 % (v/w) moisture, 0.7 mm particle size, 20 % (v/w) inoculum, 1 % (w/w ... | 2016 | 27218009 |
| [isolation, identification and fermentation optimization of bacillus tequilensis pand37 producing l-aspartate α- decarboxylase]. | we screened bacteria producing l-aspartate α-decarboxylase from grapery soil and optimized the fermentation conditions. | 2016 | 27305779 |
| performance of trichlorfon degradation by a novel bacillus tequilensis strain pa f-3 and its proposed biodegradation pathway. | the novel trichlorfon (tcf)-degrading bacterium pa f-3, identified as bacillus tequilensis, was isolated from pesticide-polluted soils by using an effective screening and domesticating procedure. the tcf biodegradation pathways of pa f-3 were also systematically elucidated. as revealed by high-performance liquid chromatography, the tcf residues in the mineral salt medium demonstrated that pa f-3 can utilize tcf as its sole carbon source and reach the highest degradation of 71.1 % at an initial t ... | 2016 | 27632165 |
| physiochemical and biological characterization of novel exopolysaccharide produced by bacillus tequilensis fr9 isolated from chicken. | hetero-exopolysaccharide secreted by bacillus tequilensis fr9 (btqeps), was produced and fractioned on deae-sepharose and phenyl sepharose cl-6b column. hplc analysis revealed the existence of five monosaccharides including glucose, galactose, mannose, arabinose and xylose. ft-ir spectroscopy confirmed the presence of carboxyl and hydroxyl groups. (1)h nmr and (13)c nmr spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages. the fibrous, porous nature and ... | 2017 | 27979726 |
| purification of antilisterial peptide (subtilosin a) from novel bacillus tequilensis fr9 and demonstrate their pathogen invasion protection ability using human carcinoma cell line. | this study focuses on isolation, screening, and characterization of novel probiotics from gastrointestinal tract of free-range chicken (gallus gallus domesticus). fifty seven colonies were isolated and three isolates (fr4, fr9, and fr12) were selected and identified as lactobacillus gasseri fr4, bacillus tequilensis fr9, and l. animalis fr12 by 16s rrna sequencing. three strains were able to survive in stimulated acidic and bile conditions and inhibit the growth of pathogens. especially, fr9 exh ... | 2016 | 27990138 |
| degradation of n-alkanes and pahs from the heavy crude oil using salt-tolerant bacterial consortia and analysis of their catabolic genes. | in the present study, salt-tolerant strains, dietzia sp. hrj2, corynebacterium variabile hrj4, dietzia cinnamea hrj5 and bacillus tequilensis hrj6 were isolated from the dagang oil field, china. these strains degraded n-alkanes and polycyclic aromatic hydrocarbons (pahs) aerobically from heavy crude oil (hco) in an experiment at 37 °c and 140 rpm. the gc/ms investigation for degradation of different chain lengths of n-alkanes (c8-c40) by individual strains showed the highest degradation of c8-c1 ... | 2017 | 28315056 |
| a novel thermostable, alkaline pectate lyase from bacillus tequilensis sv11 with potential in textile industry. | an extracellular pectate lyase was purified and characterized from a uv mutant of bacillus tequilensis sv11. purification resulted in a 16.2-fold improvement in the enzyme specific activity, with approximately 40.2% yield. sds-page showed that the enzyme had two subunits with molecular masses of 135 ± 2 and 43 ± 2 kda. further, maldi-tof ms experiments revealed that the mass spectrum of the second peptide significantly (91% score) matched with the unsaturated rhamnogalacturonyl hydrolase yter os ... | 2014 | 25037351 |
| evaluation of bacillus spp. as dough starters for adhirasam - a traditional rice based fermented food of southern india. | adhirasam is a cereal based, doughnut shaped, deep fried dessert consumed in the southern regions of india. the dough used to prepare adhirasam is fermented and contains rice flour and jaggery. the aim of the present study was to characterize the cultivable bacteria associated with this fermented dough and to identify a suitable starter culture for the production of quality adhirasam. in total, one hundred and seventy bacterial isolates were recovered from de man rogosa sharp (mrs) agar, nutrien ... | 2017 | 26691480 |
| dynamics of bacterial class bacilli in the deepest valley lake of kashmir-the manasbal lake. | in recognition of the importance of bacteria as ecological indicators of the aquatic systems a comprehensive and systematic analysis was carried out on manasbal lake, the deepest spring fed valley lake of kashmir. the main objective envisaged was to analyze bacterial community composition (bcc) and for this purpose systematic and regular sampling of waters from ten different sampling stations, predetermined in the lake according to differences in degree of human interference and also as zones of ... | 2017 | 28087491 |
| diversity, antimicrobial and antioxidant activities of culturable bacterial endophyte communities in aloe vera. | twenty-nine culturable bacterial endophytes were isolated from surface-sterilized tissues (root, stem and leaf) of aloe vera and molecularly characterized to 13 genera: pseudomonas, bacillus, enterobacter, pantoea, chryseobacterium, sphingobacterium, aeromonas, providencia, cedecea, klebsiella, cronobacter, macrococcus and shigella. the dominant genera include bacillus (20.7%), pseudomonas (20.7%) and enterobacter (13.8%). the crude and ethyl acetate fractions of the metabolites of six isolates, ... | 2015 | 26454221 |
| isolation of endophytic plant growth-promoting bacteria associated with the halophyte salicornia europaea and evaluation of their promoting activity under salt stress. | several reports have highlighted that many plant growth-promoting endophytic bacteria (pgpe) can assist their host plants in coping with various biotic and abiotic stresses. however, information about the pgpe colonizing in the halophytes is still scarce. this study was designed to isolate and characterize pgpe from salt-accumulating halophyte salicornia europaea grown under extreme salinity and to evaluate in vitro the bacterial mechanisms related to plant growth promotion. a total of 105 isola ... | 2016 | 27447799 |
| bacillus oryzicola sp. nov., an endophytic bacterium isolated from the roots of rice with antimicrobial, plant growth promoting, and systemic resistance inducing activities in rice. | biological control of major rice diseases has been attempted in several rice-growing countries in asia during the last few decades and its application using antagonistic bacteria has proved to be somewhat successful for controlling various fungal diseases in field trials. two novel endophytic bacillus species, designated strains yc7007 and yc7010(t), with anti-microbial, plant growth-promoting, and systemic resistance-inducing activities were isolated from the roots of rice in paddy fields at ji ... | 2015 | 26060434 |
| purification and characterization of an extracellular, thermo-alkali-stable, metal tolerant laccase from bacillus tequilensis sn4. | a novel extracellular thermo-alkali-stable laccase from bacillus tequilensis sn4 (sn4lac) was purified to homogeneity. the laccase was a monomeric protein of molecular weight 32 kda. uv-visible spectrum and peptide mass fingerprinting results showed that sn4lac is a multicopper oxidase. laccase was active in broad range of phenolic and non-phenolic substrates. catalytic efficiency (kcat/km) showed that 2, 6-dimethoxyphenol was most efficiently oxidized by the enzyme. the enzyme was inhibited by ... | 2014 | 24871763 |
| bacillus tequilensis sp. nov., isolated from a 2000-year-old mexican shaft-tomb, is closely related to bacillus subtilis. | a gram-positive, spore-forming bacillus was isolated from a sample taken from an approximately 2000-year-old shaft-tomb located in the mexican state of jalisco, near the city of tequila. tentative identification using conventional biochemical analysis consistently identified the isolate as bacillus subtilis. dna isolated from the tomb isolate, strain 10b(t), and closely related species was used to amplify a bacillus-specific portion of the highly conserved 16s rrna gene and an internal region of ... | 2006 | 16825615 |
| application of lipopeptide biosurfactant isolated from a halophile: bacillus tequilensis ch for inhibition of biofilm. | biosurfactants are amphiphilic molecules having hydrophobic and hydrophilic moieties produced by various microorganisms. these molecules trigger the reduction of surface tension or interfacial tension in liquids. a biosurfactant-producing halophile was isolated from lake chilika, a brackish water lake of odisha, india (19°41'39″n, 85°18'24″e). the halophile was identified as bacillus tequilensis ch by biochemical tests and 16s rrna gene sequencing and assigned accession no. kc851857 by genbank. ... | 2013 | 23955294 |
| pcr detection of ansa from marine bacteria and its sequence characteristics from bacillus tequilensis nios4. | as many as 71 marine bacterial dna extracts were pcr screened for l-asparaginase (ansa), a key gene in anti-cancer molecular-searches. over 62% (44) of them were positive for ansa gene. the positive cultures were from genera bacillus and staphylococcus. the ansa gene cloned from isolate nios4 belonging to recently described bacillus tequilensis is 1099 bp in length with a 990 bp orf coding for 329 amino acids. blastx analysis revealed this sequence to be 98% similar to earlier reported ansa sequ ... | 2014 | 23553590 |
| an assessment on the role of endophytic microbes in the therapeutic potential of fagonia indica. | natural products of animals, plants and microbes are potential source of important chemical compounds, with diverse applications including therapeutics. endophytic bacteria that are especially associated with medicinal plants presents a reservoir of therapeutic compounds. fagonia indica has been recently investigated by numerous researchers because of its striking therapeutic potential especially in cancer. it is also reported that endophytes play a vital role in the biosynthesis of various meta ... | 2017 | 28764775 |
| novel multifunctional plant growth-promoting bacteria in co-compost of palm oil industry waste. | previously, a unique co-compost produced by composting empty fruit bunch with anaerobic sludge from palm oil mill effluent, which contributed to establishing a zero-emission industry in malaysia. little was known about the bacterial functions during the composting process and fertilization capacity of this co-compost. we isolated 100 strains from the co-compost on 7 types of enumeration media and screened 25 strains using in vitro tests for 12 traits, grouping them according to three functions: ... | 2017 | 28736147 |
| highly effective bacterial agents against cimbex quadrimaculatus (hymenoptera: cimbicidae): isolation of bacteria and their insecticidal activities. | cimbex quadrimaculatus (hymenoptera: cimbicidae) is one of the serious pests of almonds in turkey and worldwide. since there is no effective control application against this pest, it has been a serious problem up to now. therefore, we aimed to find an effective bacterium that can be utilized as a biocontrol agent against c. quadrimaculatus in pest management. we isolated seven bacteria from dead and live c. quadrimaculatus larvae, and evaluated the larvicidal potency of all isolates on the respe ... | 2015 | 25358421 |