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complete nucleotide sequence of a strawberry isolate of beet pseudoyellows virus.in our effort to identify the causal agent(s) of strawberry pallidosis we found a single pallidosis positive plant that did not give any amplicons after rt-pcr using primer sets representing multiple regions of strawberry pallidosis associated virus (spav) genome and failed to react with antibodies directed against the recombinant coat protein (cp) of spav. dsrna extracted from this plant showed a similar pattern to that of spav indicating that another crinivirus may infect strawberry. sequence ...200415266105
nucleotide sequence, genome organization and phylogenetic analysis of strawberry pallidosis associated virus, a new member of the genus crinivirus.the complete nucleotide sequence of strawberry pallidosis associated virus (spav), a newly identified member of the genus crinivirus, family closteroviridae has been determined. rna 1 is 8067 nucleotides long and encodes at least three open reading frames (orfs). the first orf (orf 1a) specifies a multifunctional protein that has papain-like proteinase, methyltransferase and rna helicase domains. the rna-dependent-rna polymerase is encoded in orf 1b and is probably expressed by a +1 ribosomal fr ...200515503221
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