| vasodilatory activity in horsefly and deerfly salivary glands. | salivary gland extract (sge) of four horsefly species (hybomitra bimaculata macquart, hybomitra ciureai séguy, tabanus bromius l., tabanus glaucopis meigen) and one deerfly species (chrysops relictus meigen) (diptera: tabanidae) were shown to contain vasodilatory activity. aliquots equivalent to 1, 5 and 10 pairs of salivary glands (sg) relaxed rat femoral artery (with intact endothelium) pre-constricted with phenylephrine. vasodilatory activity was dose-dependent. sge of one horsefly species (h ... | 2003 | 14651653 |
| vasotab, a vasoactive peptide from horse fly hybomitra bimaculata (diptera, tabanidae) salivary glands. | horse flies feed from superficial haematomas and probably rely heavily on the pharmacological properties of their saliva to find blood. here we describe the first evidence of vasodilators in horse fly hybomitra bimaculata (diptera, tabanidae) salivary gland extract and clone and express one of the active peptides (termed vasotab). physiological tests using crude salivary gland extracts and reverse-phase hplc fractions demonstrated positive inotropism in isolated rat hearts, vasodilatation of cor ... | 2006 | 16391356 |
| response of tabanidae (diptera) to different natural attractants. | the response of female tabanids to natural attractants was studied in the monjoros forest along the nature park kopacki rit in eastern croatia. tabanids were caught in canopy traps baited with either aged cow, horse, sheep, or pig urine and also in unbaited traps. tabanids were collected in a significantly higher numbers in traps baited with natural attractants compared to unbaited traps. the number of females of tabanus bromius, tabanus maculicornis, tabanus tergestinus, and hybomitra bimaculat ... | 2006 | 17249343 |
| effects of horsefly (tabanidae) salivary gland extracts on isolated perfused rat heart. | the speed with which horseflies (diptera: tabanidae) obtain a bloodmeal suggests they have potent vasodilators. we used isolated perfused rat heart to examine the vasoactivity of salivary gland extracts (sges) of three horsefly species, hybomitra bimaculata macquart, tabanus bromius linnaeus and tabanus glaucopis meigen. administration of horsefly sges to the heart produced biphasic coronary responses: a decrease and subsequent increase in coronary flow (cf), characterized by initial vasoconstri ... | 2007 | 18092977 |
| effect of saliva from horse fly hybomitra bimaculata on kinetic properties of na,k-atpase: possible role in regulation of relaxation. | the possible involvement of salivary gland extract (sge) from horse flies in modifying hyperpolarization and relaxation via alterations in functional properties of sarcolemmal na,k-atpase in the host tissue was tested in vitro by application of various amounts of sge from hybomitra bimaculata.sge in the amount of 3 µg proteins representing approximately the equivalent of one salivary gland of hybomitra bimaculata induced a stimulatory effect on na,k-atpase at all atp concentrations applied. this ... | 2011 | 22058657 |
| toward an understanding of the molecular mechanism for successful blood feeding by coupling proteomics analysis with pharmacological testing of horsefly salivary glands. | horseflies are economically important blood-feeding arthropods and also a nuisance for humans and vectors for filariasis. they rely heavily on the pharmacological properties of their saliva to get a blood meal and suppress immune reactions of hosts. little information is available on antihemostatic substances in horsefly salivary glands; especially no horsefly immune suppressants have been reported. by proteomics or peptidomics and coupling transcriptome analysis with pharmacological testing, se ... | 2008 | 18087067 |
| [investigation of the sensory organs on antennae of the horseflies hybomitra bimaculata and tabanus bovinus (diptera: tabanidae) by scanning electron microscope]. | sensory organs on the antennae of the horseflies hybomitra bimaculata macq. and tabanus bovinus loew are represented by the same morphological types of sensilla. never differences in the topographical distribution of the sensilla on antennae have been also found, which can be explained by the similarity of ecological and behavioural adaptations of these insects. first and second antennal segments are found to be supplied with tactile hairs and proprioceptors. other antennal segments bear sensory ... | 2008 | 18052004 |
| leucine transport in brush border membrane vesicles from freshwater insect larvae. | leucine transport across brush border membrane vesicles prepared from four insect species common to european freshwater streams has been characterized. the species studied were: ephemera danica (ephemeroptera: ephemeridae), isoperla grammatica (plecoptera: perlodidae), hydropsyche pellucidula (trichoptera: hydropsychidae), and hybomitra bimaculata (diptera: tabanidae). the transport differed among the studied taxa for several features, including ph and sodium dependence, substrate affinity and s ... | 2006 | 17048243 |