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analysis of serpentinophytes from north-east of portugal for trace metal accumulation--relevance to the management of mine environment.in north-east of portugal, the serpentinized area is about 8000 ha with a characteristic geology and flora. the serpentine plant community and respective soils were analyzed to examine the trace metal budget in different tissues of the plants exhibiting resistance to trace metals. one hundred and thirty five plant species belonging to 39 families and respective soils have been analyzed for total co, cr, cu, fe, mn, ni, pb and zn. substantial amounts of ni, cr, co and mn were detected in plant ti ...200414675842
isolation and characterization of ni mobilizing pgpb from serpentine soils and their potential in promoting plant growth and ni accumulation by brassica spp.the study was undertaken to assess the effects of ni mobilizing bacteria on the plant growth and the uptake of ni by brassica juncea and brassica oxyrrhina. among a collection of ni resistant bacterial strains isolated from the non-rhizosphere and rhizosphere soils of alyssum serpyllifolium and astragalus incanus at a serpentine site in bragança, north-east of portugal, nine strains were selected based on their ability to solubilize ni in soil. further assessment on plant growth-promoting parame ...200919232424
Inoculation of endophytic bacteria on host and non-host plants--effects on plant growth and Ni uptake.Among a collection of Ni resistant endophytes isolated from the tissues of Alyssum serpyllifolium, four plant growth promoting endophytic bacteria (PGPE) were selected based on their ability to promote seedling growth in roll towel assay. Further, the PGPE screened showed the potential to produce plant growth promoting (PGP) substances and plant polymer hydrolyzing enzymes. These isolates were further screened for their PGP activity on A. serpyllifolium and Brassica juncea under Ni stress using ...201121872991
nickel solubilizing capacity and characterization of rhizobacteria isolated from hyperaccumulating and non-hyperaccumulating subspecies of alyssum serpyllifolium.bacterial strains were isolated from the rhizosphere of three populations of the ni-hyperaccumulator alyssum serpyllifolium subsp. lusitanicum (a. pintodasilvae; m, s, and l), one population of ni-hyperaccumulator a. serpyllifolium subsp. malacitanum (a. malacitanum; sb), and one population of the non-hyperaccumulator a. serpyllifolium subsp. serpyllifolium (a. serpyllifolium; sn). isolates were characterized genotypically by box-pcr genomic dna fingerprinting and comparative sequence analysis o ...201122046762
waste or substrate for metal hyperaccumulating plants - the potential of phytomining on waste incineration bottom ash.phytomining could represent an innovative low-cost technology for the selective recovery of valuable trace elements from secondary resources. in this context the potential of phytomining from waste incineration bottom ash was tested in a pot experiment. fresh bottom ash was acidified, leached to reduce salinity and amended with organic material to obtain a suitable substrate for plant growth. two hyperaccumulator species, alyssum serpyllifolium subsp. lusitanicum and sedum plumbizincicola as wel ...201727726916
organic amendments for improving biomass production and metal yield of ni-hyperaccumulating plants.ni phytomining is a promising technology for ni recovery from low-grade ores such as ultramafic soils. metal-hyperaccumulators are good candidates for phytomining due to their extraordinary capacity for ni accumulation. however, many of these plants produce a low biomass, which makes the use of agronomic techniques for improving their growth necessary. in this study, the ni hyperaccumulators alyssum serpyllifolium ssp. lusitanicum, a. serpyllifolium ssp. malacitanum, alyssum bertolonii and nocca ...201626803735
evolution of nickel hyperaccumulation and serpentine adaptation in the alyssum serpyllifolium species complex.metal hyperaccumulation is an uncommon but highly distinctive adaptation found in certain plants that can grow on metalliferous soils. here we review what is known about evolution of metal hyperaccumulation in plants and describe a population-genetic analysis of the alyssum serpyllifolium (brassicaceae) species complex that includes populations of nickel-hyperaccumulating as well as non-accumulating plants growing on serpentine (s) and non-serpentine (ns) soils, respectively. to test whether the ...201727782119
nickel speciation in the xylem sap of the hyperaccumulator alyssum serpyllifolium ssp. lusitanicum growing on serpentine soils of northeast portugal.nickel speciation was studied in the xylem sap of alyssum serpyllifolium ssp. lusitanicum, a ni-hyperaccumulator endemic to the serpentine soils of northeast portugal. the xylem sap was collected from plants growing in its native habitat and characterized in terms of carboxylic and amino acids content. the speciation of nickel was studied in model and real solutions of xylem sap by voltammetric titrations using square wave voltammetry (swv). the results showed that ni transport in the xylem sap ...201121664714
rhizosphere microbial densities and trace metal tolerance of the nickel hyperaccumulator alyssum serpyllifolium subsp. lusitanicum.in this study we determine culturable microbial densities (total heterotrophs, ammonifiers, amylolytics and cellulolytics) and bacterial resistance to co, cr, and ni in bulk and rhizosphere soils of three populations of the ni-hyperaccumulator alyssum serpyllifolium subsp. lusitanicum and the excluder dactylis glomerata from ultramafic sites (two populations in northeast (ne) portugal (samil (s), morais (m)) and one population in northwest (nw) spain (melide (l)). the relationship between bioava ...200919810353
improvement of plant growth and nickel uptake by nickel resistant-plant-growth promoting bacteria.in this study, among a collection of ni-resistant bacterial strains isolated from the rhizosphere of alyssum serpyllifolium and phleum phleoides grown on serpentine soil, five plant growth-promoting bacteria (pgpb) were selected based on their ability to utilize 1-aminocyclopropane-1-carboxylate (acc) as the sole n source and promote seedling growth. all of the strains tested positive for indole-3-acetic acid (iaa) production and phosphate solubilization. in addition, four of the strains exhibit ...200919147283
a phytogeochemical study of the trás-os-montes region (ne portugal): possible species for plant-based soil remediation technologies.phytoremediation techniques are now considered to be promising alternatives to conventional techniques for the remediation of diffused or moderately contaminated soils. despite their growing acceptance relatively few plant species have been studied for phytoremediation purposes. further geobotanical surveys and plant screenings are necessary since these could lead to the identification of additional species with potential value for such applications. serpentine areas could prove valuable sources ...200616399000
metal extraction by alyssum serpyllifolium ssp. lusitanicum on mine-spoil soils from spain.the efficiency of alyssum serpyllifolium ssp. lusitanicum (brassicaceae) for use in phytoextraction of polymetallic contaminated soils was evaluated. a. serpyllifolium was grown on two mine-spoil soils (ms1 and ms2): ms1 is contaminated with cr (283 mg kg(-1)) and ms2 is moderately contaminated with cr (263 mg kg(-1)), cu (264 mg kg(-1)), pb (1433 mg kg(-1)) and zn (377 mg kg(-1)). soils were limed to about ph 6.0 (ms1/ca and ms2/ca) or limed and amended with npk fertilisers (ms1/npk and ms2/npk ...200515589245
bacterially induced weathering of ultramafic rock and its implications for phytoextraction.the bioavailability of metals in soil is often cited as a limiting factor of phytoextraction (or phytomining). bacterial metabolites, such as organic acids, siderophores, or biosurfactants, have been shown to mobilize metals, and their use to improve metal extraction has been proposed. in this study, the weathering capacities of, and ni mobilization by, bacterial strains were evaluated. minimal medium containing ground ultramafic rock was inoculated with either of two arthrobacter strains: la44 ...201323793627
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