| formosa algae gen. nov., sp. nov., a novel member of the family flavobacteriaceae. | four light-yellow-pigmented, gram-negative, short-rod-shaped, non-motile isolates were obtained from enrichment culture during degradation of the thallus of the brown alga fucus evanescens. the isolates studied were chemo-organotrophic, alkalitolerant and mesophilic. polar lipids were analysed and phosphatidylethanolamine was the only phospholipid identified. the predominant cellular fatty acids were 15 : 0, i15 : 0, ai15 : 0, i15 : 1 and 15 : 1(n-6). the dna g+c contents of the four strains wer ... | 2004 | 15143012 |
| bizionia paragorgiae gen. nov., sp. nov., a novel member of the family flavobacteriaceae isolated from the soft coral paragorgia arborea. | a novel marine bacterium isolated from the soft coral paragorgia arborea in the sea of okhotsk was studied by using a polyphasic taxonomic approach. the strain, kmm 6029t, was strictly aerobic, heterotrophic, yellow-pigmented, non-motile by gliding, gram-negative and oxidase-, catalase- and alkaline phosphatase-positive. from results of 16s rrna gene sequence analysis, strain kmm 6029t occupied a distinct lineage within the family flavobacteriaceae and showed 95.5 % similarity to its closest rel ... | 2005 | 15653903 |
| formosa agariphila sp. nov., a budding bacterium of the family flavobacteriaceae isolated from marine environments, and emended description of the genus formosa. | two marine, heterotrophic, aerobic, yellow-pigmented, agarolytic bacterial strains that are motile by means of gliding were isolated from the green alga acrosiphonia sonderi and from sea water. comparative 16s rrna gene sequence analysis revealed an affiliation between the strains studied and the genus formosa, a member of the family flavobacteriaceae. the level of sequence similarity between strain kmm 3901t and formosa algae kmm 3553t was 99.1 %. the results of dna-dna hybridization experiment ... | 2006 | 16403882 |
| yeosuana aromativorans gen. nov., sp. nov., a mesophilic marine bacterium belonging to the family flavobacteriaceae, isolated from estuarine sediment of the south sea, korea. | a marine bacterium, gw1-1t, capable of degrading benzo[a]pyrene (bap), was isolated from estuarine sediments of the south sea (the korea strait), korea, after an enrichment culture maintained for 2 years in a medium supplemented with a mixture of bap and pyrene. the strain formed yellowish-brown colonies on marine agar 2216. cells were strictly aerobic, non-motile, gram-negative rods and produced non-diffusible carotenoid pigments. optimal growth occurred in the presence of 1 % (w/v) nacl and at ... | 2006 | 16585684 |
| predominance of roseobacter, sulfitobacter, glaciecola and psychrobacter in seawater collected off ushuaia, argentina, sub-antarctica. | bacterial diversity in sub-antarctic seawater, collected off ushuaia, argentina, was examined using a culture independent approach. the composition of the 16s rrna gene libraries from seawater and seawater contaminated with the water soluble fraction of crude oil was statistically different (p value 0.001). in both libraries, clones representing the alphaproteobacteria, gammaproteobacteria, the cytophaga-flavobacterium-bacteroidetes group and unculturable bacteria were dominant. clones associate ... | 2007 | 17026513 |
| formosa spongicola sp. nov., isolated from the marine sponge hymeniacidon flavia. | a gram-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-flagellated, oxidase- and catalase-positive, marine bacterium, designated a2(t), was isolated from a marine sponge, hymeniacidon flavia, collected from the coast of jeju island, south korea. phylogenetic analysis based on nearly complete 16s rrna gene sequences revealed that strain a2(t) was a member of the family flavobacteriaceae. its closest relatives were formosa agariphila kmm 3901(t) and formosa algae kmm 3553(t) (96.99 a ... | 2011 | 20228211 |
| a new recombinant endo-1,3-β-d-glucanase from the marine bacterium formosa algae kmm 3553: enzyme characteristics and transglycosylation products analysis. | a specific endo-1,3-β-d-glucanase (gfa) gene was found in genome of marine bacterium formosa algae kmm 3553. for today this is the only characterized endo-1,3-β-d-glucanase (ec 3.2.1.39) in formosa genus and the only bacterial ec 3.2.1.39 gh16 endo-1,3-β-d-glucanase with described transglycosylation activity. it was expressed in e. coli and isolated in homogeneous state. investigating the products of polysaccharides digestion with gfa allowed to establish it's substrate specificity and classify ... | 2017 | 28120311 |
| expression and biochemical characterization and substrate specificity of the fucoidanase from formosa algae. | a gene that encodes fucoidanase ffa2 in the marine bacterium formosa algae strain kmm 3553(t) was cloned, and the protein (ffa2) was produced in escherichia coli recombinant fucoidanase ffa2 was purified, and the biochemical properties of this enzyme were studied. the amino acid sequence of ffa2 showed 57% identity with known fucoidanase fcna from mariniflexile fucanivorans. the mass of the gene product ffa2 is 101.2 kda (918 amino acid residues). sequence analysis has revealed that fucoidanase ... | 2016 | 28031251 |
| formosa arctica sp. nov., isolated from arctic seawater. | a gram-staining-negative, chemoheterotrophic, yellow-pigmented, gliding, catalase- and oxidase-positive, flexirubin-negative, strictly aerobic bacterium, designated strain imcc9485(t), was isolated from a seawater sample collected from the arctic ocean. optimal growth of strain imcc9485(t) was observed at 25 °c, ph 7-8 and in the presence of 1.5-2.5 % nacl. phylogenetic analysis based on 16s rrna gene sequences showed that strain imcc9485(t) belonged to the genus formosa and was closely related ... | 2014 | 24021725 |
| formosa undariae sp. nov., isolated from a reservoir containing the brown algae undaria pinnatifida. | a strain of gram-staining-negative, aerobic, non-flagellated, non-gliding and rod-shaped bacteria, designated ws-my3(t), was isolated from a brown algae reservoir in south korea. strain ws-my3(t) grew optimally at 25 °c, at ph 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) nacl. phylogenetic trees based on 16s rrna gene sequences showed that strain ws-my3(t) fell within the cluster comprising the type strains of species of the genus formosa, clustering coherently with the type strains of formosa ... | 2013 | 23749280 |
| formosa haliotis sp. nov., a brown-alga-degrading bacterium isolated from the gut of the abalone haliotis gigantea. | four brown-alga-degrading, gram-stain-negative, aerobic, non-flagellated, gliding and rod-shaped bacteria, designated lmg 28520t, lmg 28521, lmg 28522 and lmg 28523, were isolated from the gut of the abalone haliotis gigantea obtained in japan. the four isolates had identical random amplified polymorphic dna patterns and grew optimally at 25 °c, at ph 6.0-9.0 and in the presence of 1.0-4.0 % (w/v) nacl. phylogenetic trees based on 16s rrna gene sequences placed the isolates in the genus formosa ... | 2015 | 26354496 |
| hydrolysis of fucoidan by fucoidanase isolated from the marine bacterium, formosa algae. | intracellular fucoidanase was isolated from the marine bacterium, formosa algae strain kmm 3553. the first appearance of fucoidan enzymatic hydrolysis products in a cell-free extract was detected after 4 h of bacterial growth, and maximal fucoidanase activity was observed after 12 h of growth. the fucoidanase displayed maximal activity in a wide range of ph values, from 6.5 to 9.1. the presence of mg2+, ca2+ and ba2+ cations strongly activated the enzyme; however, cu2+ and zn2+ cations had inhib ... | 2013 | 23852092 |