| the complete nucleotide sequence and its organization of the genome of barley yellow dwarf virus-gav. | the complete nucleotide sequence of genomic rna of bydv-gav was determined. it comprised 5685 nucleotides and contained six open reading frames and four un-translated regions. the size and organization of bydv-gav genome were similar to those of bydv pav-aus. the nucleotide and deduced amino acid sequences of the six orfs were aligned and compared with those of other luteoviruses. the results showed that there was a high degree of identity between bydv-gav and mav-ps1 in all orfs except orf5 and ... | 2004 | 15379250 |
| expression of the barley yellow dwarf virus-gav movement protein and its detection in the infected and transgenic plants. | movement proteins (mps) that facilitate virus movement in the plants were identified in a number of plant viruses. in this study, full-length mp gene of the chinese isolate barley yellow dwarf virus-gav (bydv-gav) was cloned and expressed in escherichia coli. about 32% of the expressed mp was soluble providing the concentration of isopropyl-beta-d-galactopyranoside (iptg), time of the induction, temperature and shaking speed were optimized. the soluble mp was purified using nickel-affinity colum ... | 2007 | 18076305 |
| [expression and subcellular localization of the orf4 gene of barley yellow dwarf virus gav strain in baculovirus-insect cell expression system]. | according to published nucleotide sequences, orf4 gene of barley yellow dwarf virus gav (bydv-gav) was synthesized by reverse transcription-polymerase chain reaction (rt-pcr). the bydv-gav orf4 gene was expressed in baculovirus -insect cell expression system efficiently, and western bolt analysis confirmed its expression product. confocal laser scanning microscopy showed that gfp: orf4 fusion protein was associated with the nuclear envelope of insect cells. by expressing the n- and c-terminal re ... | 2007 | 18257245 |