| molecular evolution in bacterial endosymbionts of fungi. | the prediction that progressive coupling of host and symbiont metabolic and reproductive interests leads to reduced mixing of symbiont lineages has been verified extensively in maternally transmitted bacterial endosymbionts of insects. to test whether this prediction is also applicable to associations of bacteria with fungi, we explored patterns of molecular evolution in two lineages of mutualistic endosymbionts of fungi: the burkholderia endosymbionts of rhizopus microsporus (mucormycotina) and ... | 2010 | 19923192 |
| endobacteria affect the metabolic profile of their host gigaspora margarita, an arbuscular mycorrhizal fungus. | the aim of this paper was to understand whether the endobacterium identified as candidatus glomeribacter gigasporarum has an effect on the biology of its host, the arbuscular mycorrhizal fungus gigaspora margarita, through the study of the modifications induced on the fungal proteome and lipid profile. the availability of g. margarita cured spores (i.e. spores that do not contain bacteria), represented a crucial tool to enable the comparison between two fungal homogeneous populations in the pres ... | 2010 | 21966904 |
| The genome of the obligate endobacterium of an AM fungus reveals an interphylum network of nutritional interactions. | As obligate symbionts of most land plants, arbuscular mycorrhizal fungi (AMF) have a crucial role in ecosystems, but to date, in the absence of genomic data, their adaptive biology remains elusive. In addition, endobacteria are found in their cytoplasm, the role of which is unknown. In order to investigate the function of the Gram-negative Candidatus Glomeribacter gigasporarum, an endobacterium of the AMF Gigaspora margarita, we sequenced its genome, leading to an ~1.72-Mb assembly. Phylogenetic ... | 2012 | 21866182 |
| detection of betaproteobacteria inside the mycelium of the fungus mortierella elongata. | microscopic and molecular analyses showed the presence of endobacteria inside the mycelia of four out of twelve nitrous oxide (n(2)o)-producing fungal isolates identified as mortierella elongata. the 16s rrna gene was successfully amplified with dna extracted directly from the endobacterium-containing fungal strains and all sequences were related to that of candidatus glomeribacter gigasporarum in the family burkholderiaceae. bacterial endotoxin was detected in the endobacterium-positive fungal ... | 2010 | 21576890 |
| endobacteria affect the metabolic profile of their host gigaspora margarita, an arbuscular mycorrhizal fungus. | summary the aim of this paper was to understand whether the endobacterium identified as candidatus glomeribacter gigasporarum has an effect on the biology of its host, the arbuscular mycorrhizal fungus gigaspora margarita, through the study of the modifications induced on the fungal proteome and lipid profile. the availability of g. margarita cured spores (i.e. spores that do not contain bacteria), represented a crucial tool to enable the comparison between two fungal homogeneous populations in ... | 2010 | 20545745 |
| detection of a novel bacterium associated with spores of the arbuscular mycorrhizal fungus gigaspora margarita. | with pcr-denaturing gradient gel electrophoresis analysis, two bacterial 16s rrna gene v3 region sequences, 7a and 7b, were detected in association with the crushed spores of the arbuscular mycorrhizal fungus gigaspora margarita w.n. becker & i.r. hall 1976 maff520054. dna sequencing and phylogenetic analysis revealed that 7b was mostly related to the documented cytoplasm endosymbiotic bacterium candidatus glomeribacter gigasporarum of g. margarita, but 7a could not be confidently assigned to a ... | 2009 | 19767848 |
| the ftsz gene of the endocellular bacterium 'candidatus glomeribacter gigasporarum' is preferentially expressed during the symbiotic phases of its host mycorrhizal fungus. | the arbuscular mycorrhizal fungus (am) gigaspora margarita consistently hosts bacteria, named 'candidatus glomeribacter gigasporarum,' inside its cytoplasm. endobacteria have a positive impact on fungal fitness during the presymbiotic phase, prior to plant roots colonization. we tested the hypothesis that the endobacterium and its cell divisions depend on fungal metabolism, mirroring also the events of the fungal life cycle which are influenced by plant signals. we first cloned a fragment of fts ... | 2009 | 19245324 |
| simultaneous detection and quantification of the unculturable microbe candidatus glomeribacter gigasporarum inside its fungal host gigaspora margarita. | a combined approach based on quantitative and nested polymerase chain reaction (qpcr and npcr, respectively) has been set up to detect and quantify the unculturable endobacterium candidatus glomeribacter gigasporarum inside the spores of its fungal host gigaspora margarita. four genes were targeted, two of bacterial origin (23s rrna gene and rpob) and two from the fungus (18s rrna gene and ef1-alpha). the sensitivity of the qpcr protocol has proved to be comparable to that of npcr, both for the ... | 2008 | 18627497 |
| presymbiotic growth and sporal morphology are affected in the arbuscular mycorrhizal fungus gigaspora margarita cured of its endobacteria. | some arbuscular mycorrhizal fungi contain endocellular bacteria. in gigaspora margarita beg 34, a homogenous population of beta-proteobacteria is hosted inside the fungal spore. the bacteria, named candidatus glomeribacter gigasporarum, are vertically transmitted through fungal spore generations. here we report how a protocol based on repeated passages through single-spore inocula caused dilution of the initial bacterial population eventually leading to cured spores. spores of this line had a di ... | 2007 | 17331157 |
| vertical transmission of endobacteria in the arbuscular mycorrhizal fungus gigaspora margarita through generation of vegetative spores. | arbuscular mycorrhizal (am) fungi living in symbiotic association with the roots of vascular plants have also been shown to host endocellular rod-shaped bacteria. based on their ribosomal sequences, these endobacteria have recently been identified as a new taxon, candidatus glomeribacter gigasporarum. in order to investigate the cytoplasmic stability of the endobacteria in their fungal host and their transmission during am fungal reproduction (asexual), a system based on transformed carrot roots ... | 2004 | 15184163 |
| 'candidatus glomeribacter gigasporarum' gen. nov., sp. nov., an endosymbiont of arbuscular mycorrhizal fungi. | arbuscular mycorrhizal fungi are obligate endosymbionts that colonize the roots of almost 80 % of land plants. the present paper describes morphological and molecular data on a bacterial endosymbiont living in the cytoplasm of dormant or germinating spores and symbiotic mycelia of the fungal species gigaspora margarita, scutellospora persica and scutellospora castanea. pcr amplification of almost the entire 16s rrna gene of the gigaspora margarita beg 34 endosymbiont, using universal bacterial p ... | 2003 | 12656162 |
| isolation, free-living capacities, and genome structure of "candidatus glomeribacter gigasporarum," the endocellular bacterium of the mycorrhizal fungus gigaspora margarita. | "candidatus glomeribacter gigasporarum" is an endocellular beta-proteobacterium present in the arbuscular mycorrhizal (am) fungus gigaspora margarita. we established a protocol to isolate "ca. glomeribacter gigasporarum" from its host which allowed us to carry out morphological, physiological, and genomic investigations on purified bacteria. they are rod shaped, with a cell wall typical of gram-negative bacteria and a cytoplasm rich in ribosomes, and they present no flagella or pili. isolated ba ... | 2004 | 15466041 |