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forest health conditions in north america.some of the greatest forest health impacts in north america are caused by invasive forest insects and pathogens (e.g., emerald ash borer and sudden oak death in the us), by severe outbreaks of native pests (e.g., mountain pine beetle in canada), and fires exacerbated by changing climate. ozone and n and s pollutants continue to impact the health of forests in several regions of north america. long-term monitoring of forest health indicators has facilitated the assessment of forest health and sus ...200818479794
antennal transcriptome analysis of odorant reception genes in the red turpentine beetle (rtb), dendroctonus valens.the red turpentine beetle (rtb), dendroctonus valens leconte (coleoptera: curculionidae, scolytinae), is a destructive invasive pest of conifers which has become the second most important forest pest nationwide in china. dendroctonus valens is known to use host odors and aggregation pheromones, as well as non-host volatiles, in host location and mass-attack modulation, and thus antennal olfaction is of the utmost importance for the beetles' survival and fitness. however, information on the genes ...201525938508
genome of the asian longhorned beetle (anoplophora glabripennis), a globally significant invasive species, reveals key functional and evolutionary innovations at the beetle-plant interface.relatively little is known about the genomic basis and evolution of wood-feeding in beetles. we undertook genome sequencing and annotation, gene expression assays, studies of plant cell wall degrading enzymes, and other functional and comparative studies of the asian longhorned beetle, anoplophora glabripennis, a globally significant invasive species capable of inflicting severe feeding damage on many important tree species. complementary studies of genes encoding enzymes involved in digestion o ...201627832824
towards the development of an autocontamination trap system to manage populations of emerald ash borer (coleoptera: buprestidae) with the native entomopathogenic fungus, beauveria bassiana.emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae) is an invasive species from asia that was discovered in north america canada, in 2002. herein, we describe studies to develop an autocontamination trapping system to disseminate beauveria bassiana to control beetle populations. the standard trap for emerald ash borer in canada is a light green prism trap covered in an insect adhesive and baited with (z)-3-hexenol. we compared of green multifunnel traps, green intercept pa ...201223356056
antioxidant genes of the emerald ash borer (agrilus planipennis): gene characterization and expression profiles.phytophagous insects frequently encounter reactive oxygen species (ros) from exogenous and endogenous sources. to overcome the effect of ros, insects have evolved a suite of antioxidant defense genes such as superoxide dismutase (sod), catalase (cat), ascorbate peroxidase (apx) and glutathione peroxidase (gpx). the emerald ash borer (agrilus planipennis fairmaire), an exotic invasive insect pest from asia has killed millions of ash trees and continues to invade north america at a rapid pace. fro ...201121439289
morphology, molecular characteristics and prevalence of a cystosporogenes species (microsporidia) isolated from agrilus anxius (coleoptera: buprestidae).a microsporidium was isolated from the bronze birch borer, agrilus anxius gory (coleoptera: buprestidae), collected near sudbury and sault ste marie, canada. light and electron microscopic investigations showed that gross pathology and ultrastructure of the investigated cystosporogenes species was similar to those characterized and described for other cystosporogenes species. small subunit rrna gene sequence data and comparative phylogenetic analysis confirmed that the microsporidian species fro ...201021167169
effects of temperature and photoperiod on the reproductive biology and diapause of oobius agrili (hymenoptera: encyrtidae), an egg parasitoid of emerald ash borer (coleoptera: buprestidae).oobius agrili zhang and huang (hymenoptera: encyrtidae) is a solitary egg parasitoid of the invasive emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae), and has been introduced to the united states for classical biological control. we characterized the weekly survivorship, fecundity, and diapause patterns of both diapaused and nondiapaused populations of o. agrili under four different temperature-photophase combinations: 30°c (warm) and 20°c (cold) temperatures with both ...201627118110
incidence of bark- and wood-boring insects in firewood: a survey at michigan's mackinac bridge.firewood is a major pathway for the inadvertent movement of bark- and wood-infesting insects. after discovery of agrilus planipennis fairmaire (coleoptera: buprestidae) in southeastern michigan in 2002, quarantines were enacted including prohibition of transporting firewood across the mackinac bridge between michigan's lower and upper peninsulas. drivers are required to surrender firewood before crossing the bridge. we surveyed recently surrendered firewood in april, july, and september 2008 and ...201021061968
susceptibility of agrilus planipennis (coleoptera: buprestidae) to beauveria bassiana and metarhizium anisopliae.the susceptibility of agrilus planipennis fairmaire (coleoptera: buprestidae) to selected strains of the entomopathogenic fungi beauveria bassiana (balsamo) vuillemin and metarhizium anisopliae (metschnikoff) sorokin was evaluated through bioassays with direct immersion or foliar exposure under laboratory conditions. results showed that a. planipennis adults were susceptible to b. bassiana and m. anisoplae. significant time-mortality response was found for each isolates. isolate b. bassiana gha ...200616937660
interaction of visual and chemical cues in promoting attraction of agrilus planipennis.female emerald ash borers, agrilus planipennis (coleoptera: buprestidae), emit a macrocyclic lactone, (z)-3-dodecen-12-olide, that increases field trap captures on large-panel prism traps when co-emitted with the green leaf volatile (z)-3-hexenol. we assessed attraction to these compounds by using visual decoy-baited branch traps, which attract males by mimicking a living female resting upon a leaf. pairs of branch traps, with and without visual decoy beetles, were placed on green ash, fraxinus ...201627234006
plan or get slam'ed: optimal management of invasive species in the presence of indirect health externalities.this paper examines invasive species management when invasive species impact health outcomes indirectly through changes to environmental quality. for example, the emerald ash borer (eab) has destroyed millions of ash trees throughout north america and has the potential to impact rates of cardiorespiratory mortality and morbidity through ash trees' ability to capture airborne pollutants. optimal management inclusive of indirect health externalities may be different than status quo plans because t ...201627318589
efficacy of soil-applied neonicotinoid insecticides for long-term protection against emerald ash borer (coleoptera: buprestidae).protection of green ash trees (fraxinus pennsylvanica marshall) from the emerald ash borer (eab), agrilus planipennis fairmaire, by soil applications of neonicotinoids (imidacloprid, clothianidin, and dinotefuran) was tested at five locations between 2005 and 2013. application rate and spring versus fall application dates were evaluated in tests with neighborhood street trees and in one plantation of 65 ash trees. insecticide treatments of ash trees at all five sites were initiated as the leadin ...201526453723
the history of attack and success of emerald ash borer (coleoptera: buprestidae) on white fringetree in southwestern ohio.emerald ash borer, agrilus planipennis fairmaire, is an invasive insect that has caused widespread mortality of ash species in north america. the ability of emerald ash borer to utilize white fringetree as an alternate host was reported recently. we aimed to determine how long white fringetree has been under attack from emerald ash borer, the degree of attack, and the overall success of this beetle on this novel host. stems from three of nine infested white fringetrees collected from the dayton ...201627325628
comparison of color attractiveness for agrilus ruficollis (coleoptera: buprestidae): potential for a simple trap.the rednecked cane borer, agrilus ruficollis (f.), is a pest of cultivated and wild blackberries in the midwestern and eastern parts of the united states. damage from this pest occurs from larvae girdling primocanes and tunneling in the pith, forming galls that can potentially reduce yields. there is only one registered insecticide and no trap available for monitoring. paints mimicking the spectral reflectance of blackberry leaves and canes of both primocane and floricane were applied to wooden ...201627346633
differences in spectral selectivity between stages of visually guided mating approaches in a buprestid beetle.spectral mating preferences were examined in male agrilus angustulus (buprestidae: coleoptera), a member of a taxon known for its high species diversity and striking metallic coloration. the spectral emission profile of a typical a. angustulus female displays low chroma, broadly overlapping that of the green oak leaves they feed and rest upon, while also including longer wavelengths. to pinpoint behaviorally significant spectral regions for a. angustulus males during mate selection, we observed ...201627401761
higher activities of defense-associated enzymes may contribute to greater resistance of manchurian ash to emerald ash borer than a closely related and susceptible congener.emerald ash borer (eab) is an invasive beetle native to asia that infests and kills ash (fraxinus spp.) in north america. previous experiments indicated that larvae feeding on co-evolved, resistant manchurian ash (f. mandshurica) have increased antioxidant and quinone-protective enzyme activities compared to larvae feeding on susceptible north american species. here, we examined mechanisms of host-generated oxidative and quinone-based stress and other putative defenses in manchurian ash and the ...201627484881
the green ash transcriptome and identification of genes responding to abiotic and biotic stresses.to develop a set of transcriptome sequences to support research on environmental stress responses in green ash (fraxinus pennsylvanica), we undertook deep rna sequencing of green ash tissues under various stress treatments. the treatments, including emerald ash borer (eab) feeding, heat, drought, cold and ozone, were selected to mimic the increasing threats of climate change and invasive pests faced by green ash across its native habitat.201627589953
genome sequence and genetic diversity of european ash trees.ash trees (genus fraxinus, family oleaceae) are widespread throughout the northern hemisphere, but are being devastated in europe by the fungus hymenoscyphus fraxineus, causing ash dieback, and in north america by the herbivorous beetle agrilus planipennis. here we sequence the genome of a low-heterozygosity fraxinus excelsior tree from gloucestershire, uk, annotating 38,852 protein-coding genes of which 25% appear ash specific when compared with the genomes of ten other plant species. analyses ...201728024298
distribution, predictors, and impacts of emerald ash borer (agrilus planipennis) (coleoptera: buprestidae) infestation of white fringetree (chionanthus virginicus).emerald ash borer, agrilus planipennis (fairmaire), is an invasive pest of ash trees (fraxinus spp.) in north america that was recently found infesting white fringetree (chionanthus virginicus l.). initial reports of the infestation of white fringetree by emerald ash borer occurred in southwestern ohio and chicago, il. we examined white fringetrees at additional sites in illinois, indiana, ohio, and pennsylvania in summer and fall 2015 and winter 2016 for emerald ash borer infestation. our aim w ...201728031427
larval survival and growth of emerald ash borer (coleoptera: buprestidae) on white ash and white fringetree saplings under well-watered and water-deficit conditions. 201628175243
behavioral responses to resource heterogeneity can accelerate biological invasions.the abundance and spatial distribution of resources in a landscape and the behavioral response of individuals determines whether and how fast an invasive species spreads in an environment. whether and how landscape manipulations can be used to slow invasive species is of great interest, in particular in forest ecosystems, where tree removal, thinning, and increasing tree diversity are discussed as management options. classically, the focus is on availability and accessibility of resources; more ...201728194758
optimizing use of girdled ash trees for management of low-density emerald ash borer (coleoptera: buprestidae) populations.effective survey methods to detect and monitor recently established, low-density infestations of emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae), remain a high priority because they provide land managers and property owners with time to implement tactics to slow emerald ash borer population growth and the progression of ash mortality. we evaluated options for using girdled ash (fraxinus spp.) trees for emerald ash borer detection and management in a low-density infesta ...201728379445
effects of introduced insects and diseases on forest ecosystems in the catskill mountains of new york.repeated invasions of non-native insects and pathogens have altered the structure and function of forest ecosystems in the catskill mountains of new york state, and will continue to do so in the future. gypsy moth, beech bark disease, and hemlock woolly adelgid are among the insects and diseases currently established in the catskills that are having significant effects on forests. many others, including emerald ash borer, asian long-horned beetle, phytophthora ramorum, and sirex wood wasp, are e ...201323844706
susceptibility of two hymenopteran parasitoids of agrilus planipennis (coleoptera: buprestidae) to the entomopathogenic fungus beauveria bassiana (ascomycota: hypocreales).emerald ash borer (eab), agrilus planipennis fairmaire, native to asia, is killing ash trees (fraxinus spp.) across 15 states and southeastern canada. integrated pest management using biological control is the only viable long-term approach for controlling the spread of eab outside of host resistance. three hymenopteran parasitoids, spathius agrili yang, tetrastichus planipennisi yang, and oobius agrili zhang and huang were discovered attacking eab in china and were approved for release in the u ...201222245471
characterization and virulence of beauveria spp. recovered from emerald ash borer in southwestern ontario, canada.the emerald ash borer (eab), agrilus planipennis (coleoptera: buprestidae), is an invasive wood boring beetle that is decimating north america's ash trees (fraxinus spp.). to find effective and safe indigenous biocontrol agents to manage eab, we conducted a survey in 2008-2009 of entomopathogenic fungi (epf) infecting eab in five outbreak sites in southwestern ontario, canada. a total of 78 beauveria spp. isolates were retrieved from dead and mycosed eab cadavers residing in the phloem tissues o ...201222664171
a molecular tool for detection and tracking of a potential indigenous beauveria bassiana strain for managing emerald ash borer populations in canada.emerald ash borer is an invasive species from asia. beauveria bassiana strain l49-1aa is being tested for the control of emerald ash borer in canada, using an autocontamination trapping system. we have developed a simplified allele discrimination polymerase chain reaction (pcr) assay to screen b. bassiana strain, l49-1aa from other beauveria species by targeting the inter-strain genetic differences in 5' end of ef1-α gene of the genus beauveria. a single nucleotide polymorphism (snp) site, t→c w ...201425110340
agrobacterium-mediated genetic transformation and plant regeneration of the hardwood tree species fraxinus profunda.this transformation and regeneration protocol provides an integral framework for the genetic improvement of fraxinus profunda (pumpkin ash) for future development of plants resistant to the emerald ash borer. using mature hypocotyls as the initial explants, an agrobacterium tumefaciens-mediated genetic transformation system was successfully developed for pumpkin ash (fraxinus profunda). this transformation protocol is an invaluable tool to combat the highly aggressive, non-native emerald ash bor ...201424493252
identification of odor-processing genes in the emerald ash borer, agrilus planipennis.insects rely on olfaction to locate food, mates, and suitable oviposition sites for successful completion of their life cycle. agrilus planipennis fairmaire (emerald ash borer) is a serious invasive insect pest that has killed tens of millions of north american ash (fraxinus spp) trees and threatens the very existence of the genus fraxinus. adult a. planipennis are attracted to host volatiles and conspecifics; however, to date no molecular knowledge exists on olfaction in a. planipennis. hence, ...201323424668
transcriptome analysis of the emerald ash borer (eab), agrilus planipennis: de novo assembly, functional annotation and comparative analysis.the emerald ash borer (eab), agrilus planipennis, is an invasive phloem-feeding insect pest of ash trees. since its initial discovery near the detroit, us- windsor, canada area in 2002, the spread of eab has had strong negative economic, social and environmental impacts in both countries. several transcriptomes from specific tissues including midgut, fat body and antenna have recently been generated. however, the relatively low sequence depth, gene coverage and completeness limited the usefulnes ...201526244979
tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (agrilus planipennis).the insect midgut and fat body represent major tissue interfaces that deal with several important physiological functions including digestion, detoxification and immune response. the emerald ash borer (agrilus planipennis), is an exotic invasive insect pest that has killed millions of ash trees (fraxinus spp.) primarily in the midwestern united states and ontario, canada. however, despite its high impact status little knowledge exists for a. planipennis at the molecular level.201021060843
decreased emergence of emerald ash borer from ash treated with methyl jasmonate is associated with induction of general defense traits and the toxic phenolic compound verbascoside.the emerald ash borer (eab; agrilus planipennis fairmaire) is causing widespread mortality of ash (fraxinus spp.) in north america. to date, no mechanisms of host resistance have been identified against this pest. methyl jasmonate was applied to susceptible north american and resistant asian ash species to determine if it can elicit induced responses in bark that enhance resistance to eab. in particular, phenolic compounds, lignin, and defense-related proteins were quantified, and compounds asso ...201425231373
a cure for the blues: opsin duplication and subfunctionalization for short-wavelength sensitivity in jewel beetles (coleoptera: buprestidae).arthropods have received much attention as a model for studying opsin evolution in invertebrates. yet, relatively few studies have investigated the diversity of opsin proteins that underlie spectral sensitivity of the visual pigments within the diverse beetles (insecta: coleoptera). previous work has demonstrated that beetles appear to lack the short-wavelength-sensitive (sws) opsin class that typically confers sensitivity to the "blue" region of the light spectrum. however, this is contrary to ...201627193495
molecular recognition of emerald ash borer infestation using leaf spray mass spectrometry.the introduction of the emerald ash borer (agrilus planipennis) (eab) from asia to michigan, usa, in the 1990s caused the widespread death of ash trees in two canadian provinces and 24 us states. the three current methods for the detection of emerald ash borer infestation, visual surveys, tree girdling and artificial traps, can be unreliable, and there is clearly a need for a rapid, dependable technique for the detection of emerald ash borer infestation.201627173112
short-term responses of ground beetles to forest changes caused by early stages of emerald ash borer (coleoptera: buprestidae)-induced ash mortality.emerald ash borer (agrilus planipennis fairmaire), an invasive wood-boring beetle native to asia, has killed hundreds of millions of ash trees since its accidental introduction into north america, resulting in widespread formation of canopy gaps and accumulations of coarse woody debris (cwd) in forests. the objective was to quantify effects of canopy gaps and cwd caused by early stages of emerald ash borer-induced ash mortality, and their interaction on ground beetle assemblages. the impact of c ...201627106817
laboratory evaluation of the toxicity of systemic insecticides to emerald ash borer larvae.emerald ash borer (agrilus planipennis fairmaire) (coleoptera: buprestidae), an invasive phloem-feeding insect native to asia, threatens at least 16 north american ash (fraxinus) species and has killed hundreds of millions of ash trees in landscapes and forests. we conducted laboratory bioassays to assess the relative efficacy of systemic insecticides to control emerald ash borer larvae in winter 2009 and 2010. second- and third-instar larvae were reared on artificial diet treated with varying d ...201626721288
white fringetree as a novel larval host for emerald ash borer.emerald ash borer is an invasive asian pest of ash species in north america. all north american species of ash tested so far are susceptible to it, but there are no published reports of this insect developing fully in non-ash hosts in the field in north america. i report here evidence that emerald ash borer can attack and complete development in white fringetree, chionanthus virginicus l., a species native to the southeastern united states that is also planted ornamentally. four of 20 mature orn ...201526470141
coupled human-environment dynamics of forest pest spread and control in a multi-patch, stochastic setting.the transportation of camp firewood infested by non-native forest pests such as asian long-horned beetle (alb) and emerald ash borer (eab) has severe impacts on north american forests. once invasive forest pests are established, it can be difficult to eradicate them. hence, preventing the long-distance transport of firewood by individuals is crucial.201526430902
is tree loss associated with cardiovascular-disease risk in the women's health initiative? a natural experiment.data from the women's health initiative were used to quantify the relationship between the loss of trees to an invasive forest pest-the emerald ash borer-and cardiovascular disease. we estimated a semi-parametric cox proportional hazards model of time to cardiovascular disease, adjusting for confounders. we defined the incidence of cardiovascular disease as acute myocardial infarction requiring overnight hospitalization, silent mi determined from serial electrocardiograms, ischemic or hemorrhagi ...201526335885
growth of larval agrilus planipennis (coleoptera: buprestidae) and fitness of tetrastichus planipennisi (hymenoptera: eulophidae) in blue ash (fraxinus quadrangulata) and green ash (f. pennsylvanica).emerald ash borer (agrilus planipennis fairmaire) is an invasive primary pest of north american ash (fraxinus spp.) trees. blue ash (f. quadrangulata) is less susceptible to emerald ash borer infestations in the forest than other species of north american ash. whereas other studies have examined adult host preferences, we compared the capacity of emerald ash borer larvae reared from emerald ash borer eggs in the field and in the laboratory to survive and grow in blue ash and the more susceptible ...201526314024
incidence of infestation and larval success of emerald ash borer (agrilus planipennis) on white fringetree (chionanthus virginicus), chinese fringetree (chionanthus retusus), and devilwood (osmanthus americanus).we compared the incidence of infestation by emerald ash borer (eab) and lilac borer on white fringetree to that of its asian congener, chinese fringetree, chionanthus retusus, and a north american relative, devilwood, osmanthus americanus. we also conducted laboratory bioassays to determine the suitability of these hosts for eab larvae. at spring grove cemetery and arboretum in cincinnati, ohio, 9 of 28 white fringetrees examined were infested by eab. most of the white fringetrees had lilac bore ...201526314014
effects of pheromone release rate and trap placement on trapping of agrilus planipennis (coleoptera: buprestidae) in canada.the emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae), is a devastating insect pest in its introduced range. a trapping system that increases trap catches or detection rates in low-density populations would be beneficial for survey programs. five trapping experiments were conducted to investigate factors influencing capture rates of male beetles on dark green traps baited with the a. planipennis pheromone, (3z)-dodecen-12-olide ((3z)-lactone), plus the green leaf volatil ...201526313980
host resistance of five fraxinus species to agrilus planipennis (coleoptera: buprestidae) and effects of paclobutrazol and fertilization.agrilus planipennis fairmaire (coleoptera: buprestidae) has killed millions of fraxinus spp. trees in north america. while all fraxinus species assessed to date can be colonized, a. planipennis attraction to host trees varies among species and with tree health. we established a plantation of 105 trees (21 trees each of four north american species fraxinus americana l., fraxinus nigra marshall, fraxinus pennsylvanica marshall, fraxinus quadrangulata michaux, and the asian species, fraxinus mandsh ...201526313182
comparison of trap types and colors for capturing emerald ash borer adults at different population densities.results of numerous trials to evaluate artificial trap designs and lures for detection of agrilus planipennis fairmaire, the emerald ash borer, have yielded inconsistent results, possibly because of different a. planipennis population densities in the field sites. in 2010 and 2011, we compared 1) green canopy traps, 2) purple canopy traps, 3) green double-decker traps, and 4) purple double-decker traps in sites representing a range of a. planipennis infestation levels. traps were baited with cis ...201424398125
influence of mortality factors and host resistance on the population dynamics of emerald ash borer (coleoptera: buprestidae) in urban forests.the success of emerald ash borer (agrilus planipennis fairmaire) in north america is hypothesized to be due to both the lack of significant natural enemies permitting easy establishment and a population of trees that lack the ability to defend themselves, which allows populations to grow unchecked. since its discovery in 2002, a number of studies have examined mortality factors of the insect in forests, but none have examined the role of natural enemies and other mortality agents in the urban fo ...201526308819
progress and gaps in understanding mechanisms of ash tree resistance to emerald ash borer, a model for wood-boring insects that kill angiosperms.we review the literature on host resistance of ash to emerald ash borer (eab, agrilus planipennis), an invasive species that causes widespread mortality of ash. manchurian ash (fraxinus mandshurica), which coevolved with eab, is more resistant than evolutionarily naïve north american and european congeners. manchurian ash was less preferred for adult feeding and oviposition than susceptible hosts, more resistant to larval feeding, had higher constitutive concentrations of bark lignans, coumarins ...201626268949
emerald ash borer and the urban forest: changes in landslide potential due to canopy loss scenarios in the city of pittsburgh, pa.emerald ash borer is expected to kill thousands of ash trees in the eastern u.s. this research develops tools to predict the effect of ash tree loss from the urban canopy on landslide susceptibility in pittsburgh, pa. a spatial model was built using the sinmap (stability index mapping) model coupled with spatially explicit scenarios of tree loss (0%, 25%, 50%, and 75% loss of ash trees from the canopy). ash spatial distributions were estimated via monte carlo methods and available vegetation plo ...201526245535
lethal trap trees: a potential option for emerald ash borer (agrilus planipennis fairmaire) management.economic and ecological impacts of ash (fraxinus spp.) mortality resulting from emerald ash borer (eab) (agrilus planipennis fairmaire) invasion are severe in forested, residential and urban areas. management options include girdling ash trees to attract ovipositing adult beetles and then destroying infested trees before larvae develop or protecting ash with a highly effective, systemic emamectin benzoate insecticide. injecting this insecticide and then girdling injected trees a few weeks later ...201626194342
biotic mortality factors affecting emerald ash borer (agrilus planipennis) are highly dependent on life stage and host tree crown condition.emerald ash borer (eab), agrilus planipennis, is a serious invasive forest pest in north america responsible for killing tens to hundreds of millions of ash trees since it was accidentally introduced in the 1990 s. although host-plant resistance and natural enemies are known to be important sources of mortality for eab in asia, less is known about the importance of different sources of mortality at recently colonized sites in the invaded range of eab, and how these relate to host tree crown cond ...201526072908
physiological responses of emerald ash borer larvae to feeding on different ash species reveal putative resistance mechanisms and insect counter-adaptations.emerald ash borer, agrilus planipennis fairmaire, an asian wood-boring beetle, has devastated ash (fraxinus spp.) trees in north american forests and landscapes since its discovery there in 2002. in this study, we collected living larvae from eab-resistant manchurian ash (fraxinus mandschurica), and susceptible white (fraxinus americana) and green (fraxinus pennsylvanica) ash hosts, and quantified the activity and production of selected detoxification, digestive, and antioxidant enzymes. we hypo ...201525956198
two new species of oobius trjapitzin (hymenoptera, encyrtidae) egg parasitoids of agrilus spp. (coleoptera, buprestidae) from the usa, including a key and taxonomic notes on other congeneric nearctic taxa.oobius trjapitzin (hymenoptera, encyrtidae) species are egg parasitoids that are important for the biological control of some buprestidae and cerambycidae (coleoptera). two species, oobiusagrili zhang & huang and oobiuslongoi (siscaro), were introduced into north america for classical biocontrol and have successfully established. two new native north american species that parasitize eggs of agrilus spp. (buprestidae) are described and illustrated from the usa: oobiusminusculus triapitsyn & petri ...201525931963
abundance of volatile organic compounds in white ash phloem and emerald ash borer larval frass does not attract tetrastichus planipennisi in a y-tube olfactometer.many natural enemies employ plant- and/or herbivore-derived signals for host/prey location. the larval parasitoid tetrastichus planipennisi yang (hymenoptera: eulophidae) is 1 of 3 biocontrol agents currently being released in an effort to control the emerald ash borer (eab), agrilus planipennis fairmaire (coloeptera: burprestidae) in north america. to enhance its efficiency, allelochemicals that attract it need to be assessed. in this study, ash phloem volatile organic compounds (vocs) of black ...201625879864
a biologically active analog of the sex pheromone of the emerald ash borer, agrilus planipennis.the emerald ash borer, agrilus planipennis (coleoptera: buprestidae) (eab), is an invasive species causing unprecedented levels of mortality to ash trees in its introduced range. the female-produced sex pheromone of eab has been shown to contain the macrocyclic lactone (3z)-dodecen-12-olide. this compound and its geometrical isomer, (3e)-dodecen-12-olide, have been demonstrated previously to be eag active and, in combination with a host-derived green leaf volatile, (3z)-hexenol, to be attractive ...201525786893
managing outbreaks of invasive species--a new method to prioritize preemptive quarantine efforts across large geographic regions.in pest risk assessment it is frequently necessary to make time-critical decisions regarding management of expanding pest populations. when an invasive pest outbreak is expanding rapidly, preemptive quarantine of areas that are under imminent threat of infestation is one of only a few available management tools that can be implemented quickly to help control the expansion. the preemptive quarantine of locations that surround an infested area also acts as a safeguard to counteract the risk of fai ...201525560653
the relationship between trees and human health: evidence from the spread of the emerald ash borer.several recent studies have identified a relationship between the natural environment and improved health outcomes. however, for practical reasons, most have been observational, cross-sectional studies.201323332329
core rnai machinery and gene knockdown in the emerald ash borer (agrilus planipennis).the rna interference (rnai) technology has been widely used in insect functional genomics research and provides an alternative approach for insect pest management. to understand whether the emerald ash borer (agrilus planipennis), an invasive and destructive coleopteran insect pest of ash tree (fraxinus spp.), possesses a strong rnai machinery that is capable of degrading target mrna as a response to exogenous double-stranded rna (dsrna) induction, we identified three rnai pathway core component ...201525541004
nutritional attributes of ash (fraxinus spp.) outer bark and phloem and their relationships to resistance against the emerald ash borer.the emerald ash borer (agrilus planipennis fairmaire, eab) is an alien, invasive wood-boring insect that is responsible for killing millions of ash trees since its discovery in north america in 2002. all north american ash species (fraxinus spp.) that eab has encountered have shown various degrees of susceptibility, while manchurian ash (fraxinus mandshurica ruprecht), which shares a co-evolutionary history with this insect, is resistant. recent studies have looked into constitutive resistance m ...201223143945
motivation for compliance with environmental regulations related to forest health.this study extends previous research on motivations for compliance with environmental regulations. it addresses contexts where regulatees have primarily sporadic short term interests, where costs of compliance are modest, and where costs of non-compliance are low. the behavior studied is the movement of firewood for camping, a principal cause for the spread of the emerald ash borer (agrilus planipennis), one of several invasive species plaguing the eastern united states. based on a three-wave ma ...201222890000
factors affecting the flight capacity of tetrastichus planipennisi (hymenoptera: eulophidae), a classical biological control agent of agrilus planipennis (coleoptera: buprestidae).the dispersal characteristics of a biological control agent can have direct implications on the ability of that agent to control populations of a target host. tetrastichus planipennisi yang (hymenoptera: eulophidae) is a parasitic wasp native to eastern asia that has been introduced into the united states as part of a classical biological control program against the emerald ash borer agrilus planipennis fairmaire (coleoptera: buprestidae). we used computer-monitored flight mills to investigate t ...201425479199
bioreplicated visual features of nanofabricated buprestid beetle decoys evoke stereotypical male mating flights.recent advances in nanoscale bioreplication processes present the potential for novel basic and applied research into organismal behavioral processes. insect behavior potentially could be affected by physical features existing at the nanoscale level. we used nano-bioreplicated visual decoys of female emerald ash borer beetles (agrilus planipennis) to evoke stereotypical mate-finding behavior, whereby males fly to and alight on the decoys as they would on real females. using an industrially scala ...201425225359
characterization of horizontally transferred β-fructofuranosidase (scrb) genes in agrilus planipennis.the emerald ash borer (agrilus planipennis) is an important invasive insect pest of fraxinus spp. that feeds on host tissues containing high levels of sucrose. however, little is known about how it digests sucrose. here, using larval midgut transcriptome data and preliminary genome sequence efforts, two β-fructofuranosidase-encoding scrb genes, aplascrb-1 and aplascrb-2, were identified, and proved to reside within the a. planipennis genome. homology and phylogenetic analysis revealed that they ...201425224649
effect of parasitoid: host ratio and group size on fitness of spathius galinae (hymenoptera: braconidae): implications for mass-rearing.producing insect natural enemies in laboratories or insectaries for biological pest control is often expensive, and developing cost-effective rearing techniques is a goal of many biological control programs. spathius galinae belokobylskij and strazenac (hymenoptera: braconidae), a newly described ectoparasitoid of emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae), is currently being evaluated for environmental introduction in the united states to provide biological contr ...201526470215
modelling interactions between forest pest invasions and human decisions regarding firewood transport restrictions.the invasion of nonnative, wood-boring insects such as the asian longhorned beetle (a. glabripennis) and the emerald ash borer (a. planipennis) is a serious ecological and economic threat to canadian deciduous and mixed-wood forests. humans act as a major vector for the spread of these pests via firewood transport, although existing models do not explicitly capture human decision-making regarding firewood transport. in this paper we present a two-patch coupled human-environment system model that ...201424736497
indirect effects of emerald ash borer-induced ash mortality and canopy gap formation on epigaeic beetles.exotic herbivorous insects have drastically and irreversibly altered forest structure and composition of north american forests. for example, emerald ash borer (agrilus planipennis fairmaire) from asia has caused wide-scale mortality of ash trees (fraxinus spp.) in eastern united states and canada. we studied the effects of forest changes resulting from emerald ash borer invasion on epigaeic or ground beetles (coleoptera: carabidae) along a gradient of ash dieback and gap sizes in southeastern m ...201424690169
motivations for rule compliance in support of forest health: replication and extension.this study replicates and extends research conducted in 2008. based on a random sample of 800 campers who used wisconsin state parks and forests in 2010, it confirms that calculated, normative, and social motivations are all important determinants of firewood movement rule compliance, a context where regulatees have primarily sporadic short-term interests, and where costs of compliance and non-compliance are both low. the study uses bi-variate statistical tests and recursive partitioning (standa ...201424681652
efficacy of heat treatment for the thousand cankers disease vector and pathogen in small black walnut logs.thousand cankers disease, caused by the walnut twig beetle (pityophthorus juglandis blackman) and an associated fungal pathogen (geosmithia morbida m. kolarík, e. freeland, c. utley, and n. tisserat), threatens the health and commercial use of eastern black walnut (juglans nigra l.), one of the most economically valuable tree species in the united states. effective phytosanitary measures are needed to reduce the possibility of spreading this insect and pathogen through wood movement. this study ...201424665700
optimization of multifunnel traps for emerald ash borer (coleoptera: buprestidae): influence of size, trap coating, and color.field assays were conducted in southeastern and south-central michigan in 2011 and 2012 to optimize green and purple multifunnel (lindgren funnel) traps for use as a survey tool for the emerald ash borer, agrilus planipennis fairmaire. larger sized (12- and 16-unit) multifunnel traps caught more beetles than their smaller-sized (4- and 8-unit) counterparts. green traps coated with untinted (white) fluon caught almost four times as many adult a. planipennis as rain-x and tinted (green) fluon-coat ...201324498742
improving detection tools for the emerald ash borer (coleoptera: buprestidae): comparison of prism and multifunnel traps at varying population densities.the current emerald ash borer survey trap used in the united states is a prism trap constructed from a stock purple corrugated plastic. in recent years, several colors (particularly shades of green and purple) have been shown to be more attractive to the emerald ash borer than this stock color. our goal was to determine if plastics produced with these colors and incorporated into prism traps can improve and serve as a new alternative to plastics already in use for the emerald ash borer survey. t ...201324498741
quantifying the impact of woodpecker predation on population dynamics of the emerald ash borer (agrilus planipennis).the emerald ash borer (eab), agrilus planipennis, is an invasive beetle that has killed millions of ash trees (fraxinus spp.) since it was accidentally introduced to north america in the 1990s. understanding how predators such as woodpeckers (picidae) affect the population dynamics of eab should enable us to more effectively manage the spread of this beetle, and toward this end we combined two experimental approaches to elucidate the relative importance of woodpecker predation on eab populations ...201324349520
a comparison of girdled ash detection trees and baited artificial traps for agrilus planipennis (coleoptera: buprestidae) detection.detection of newly established populations of agrilus planipennis fairmaire, the most destructive forest insect to invade the united states, remains challenging. regulatory agencies currently rely on artificial traps, consisting of baited three-sided panels suspended in the canopy of ash (fraxinus spp.) trees. detection trees represent another survey option. ash trees are girdled in spring to attract ovipositing a. planipennis females then debarked in fall to assess larval presence and density. ...201324331612
effects of host plant and larval density on intraspecific competition in larvae of the emerald ash borer (coleoptera: buprestidae).competition for food, mates, and space among different individuals of the same insect species can affect density-dependent regulation of insect abundance or population dynamics. the emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae), is a serious invasive pest of north american ash (fraxinus spp.) trees, with its larvae feeding in serpentine galleries between the interface of sapwood and phloem tissues of ash trees. using artificial infestation of freshly cut logs of gree ...201324280666
effects of ambient temperature on egg and larval development of the invasive emerald ash borer (coleoptera: buprestidae): implications for laboratory rearing.the emerald ash borer, agrilus planipennis fairmaire, an invasive beetle from asia causing large scale ash (fraxinus) mortality in north america, has been extremely difficult to rear in the laboratory because of its long life cycle and cryptic nature of immature stages. this lack of effective laboratory-rearing methods has not only hindered research into its biology and ecology, but also mass production of natural enemies for biological control of this invasive pest. using sticks from the altern ...201324224252
effects of water availability on emerald ash borer larval performance and phloem phenolics of manchurian and black ash.the invasive emerald ash borer (eab) beetle is a significant threat to the survival of north american ash. in previous work, we identified putative biochemical and molecular markers of constitutive eab resistance in manchurian ash, an asian species co-evolved with eab. here, we employed high-throughput high-performance liquid chromatography with photodiode array detection and mass spectrometry (hplc-pda-ms) to characterize the induced response of soluble phloem phenolics to eab attack in resista ...201424125060
emerald ash borer invasion of north america: history, biology, ecology, impacts, and management.since its accidental introduction from asia, emerald ash borer (eab), agrilus planipennis fairmaire (coleoptera: buprestidae), has killed millions of ash trees in north america. as it continues to spread, it could functionally extirpate ash with devastating economic and ecological impacts. little was known about eab when it was first discovered in north america in 2002, but substantial advances in understanding of eab biology, ecology, and management have occurred since. ash species indigenous t ...201424112110
establishment and abundance of tetrastichus planipennisi (hymenoptera: eulophidae) in michigan: potential for success in classical biocontrol of the invasive emerald ash borer (coleoptera: buprestidae).tetrastichus planipennisi yang is a gregarious larval endoparasitoid native to china and has been introduced to the united states since 2007 for classical biological control of the invasive emerald ash borer, agrilus planipennis fairmaire, an exotic beetle responsible for widespread ash mortality. between 2007-2010, t. planipennisi adults (3,311-4,597 females and approximately 1,500 males per site) were released into each of six forest sites in three counties (ingham, gratiot, and shiawassee) of ...201323865178
modeling agrilus planipennis (coleoptera: buprestidae) within-tree colonization patterns and development of a subsampling technique.emerald ash borer, agrilus planipennis fairmaire, an insect native to central asia, was first detected in southeast michigan in 2002, and has since killed millions of ash trees (fraxinus spp.) in north america. the objectives of this study were to 1) determine an optimal sampling location on girdled ash trap trees for detection of a. planipennis larvae based on measurements of tree characteristics, and 2) develop a whole-tree estimation method for extrapolating larval densities from subsampled h ...201323726062
water conservation features of ova of agrilus planipennis (coleoptera: buprestidae).the emerald ash borer, agrilus planipennis fairmaire, has destroyed millions of ash trees (fraxinus spp.) in north america since first identified in detroit in 2002. with species of ash distributed throughout north america, it is easy to speculate the extinction of all susceptible species of ash on the continent given a lack of physical, environmental, or climactic barrier for dispersal of the insect. we investigated water balance characteristics of emerald ash borer ova by using gravimetric met ...201323575027
glutathione-s-transferase profiles in the emerald ash borer, agrilus planipennis.the emerald ash borer, agrilus planipennis fairmaire is a recently discovered invasive insect pest of ash, fraxinus spp. in north america. glutathione-s-transferases (gst) are a multifunctional superfamily of enzymes which function in conjugating toxic compounds to less toxic and excretable forms. in this study, we report the molecular characterization and expression patterns of different classes of gst genes in different tissues and developmental stages plus their specific activity. multiple se ...201323499941
six new species of agrilus curtis, 1825 (coleoptera, buprestidae, agrilinae) from the oriental region related to the emerald ash borer, a. planipennis fairmaire, 1888 and synonymy of sarawakita obenberger, 1924.six new species of agrilus curtis, 1825 with affinities to the emerald ash borer, agrilus planipennis fairmaire, 1888, are described from the oriental region: agrilus crepuscularissp. n. (malaysia); agrilus pseudolubopetrisp. n. (laos); agrilus sapphirinussp. n.(laos); agrilus seramensissp. n.(indonesia); agrilus spineussp. n. (malaysia); and agrilus tomentipennissp. n. (laos). the genus sarawakita obenberger, 1924 syn. nov. is considered a junior synonym of agrilus.201223226712
critical rearing parameters of tetrastichus planipennisi (hymenoptera: eulophidae) as affected by host plant substrate and host-parasitoid group structure.in laboratory assays, we evaluated the potential impact of host plant substrate types, host-parasitoid group sizes (densities), and parasitoid-to-host ratios on select fitness parameters of the larval endoparasitoid tetrastichus planipennisi yang (hymenoptera: eulophidae), newly introduced for biological control of emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae), in the united states. results from our study showed that offspring production and critical fitness paramete ...201222812114
foliar residue dynamics of azadirachtins following direct stem injection into white and green ash trees for control of emerald ash borer.azadirachtins are natural insecticides derived from the neem tree. the emerald ash borer (eab) is an exotic invasive insect pest that infests various ash tree species and has the potential for significant economic, aesthetic and ecological impacts throughout north america. the initial translocation and foliar residue dynamics of azadirachtins were examined following direct injection into white and green ash trees growing in urban scenarios as a potential control for eab.201121567888
attraction of agrilus planipennis (coleoptera: buprestidae) to a volatile pheromone: effects of release rate, host volatile, and trap placement.attraction of emerald ash borer, agrilus planipennis fairmaire, to a volatile pheromone was demonstrated in three field experiments using baited green sticky traps. a dose-response curve was generated for male a. planipennis to increasing release rates of (3z)-dodecen-12-olide ((3z)-lactone) in combination with the green leaf volatile, (3z)-hexenol. only the lowest release rate (<2.50 μg/d) of (3z)-lactone significantly increased captures of male a. planipennis, as compared with traps baited wit ...201222732623
improving detection tools for emerald ash borer (coleoptera: buprestidae): comparison of multifunnel traps, prism traps, and lure types at varying population densities.the emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae), is a serious invasive pest of north american ash (fraxinus spp.) that has caused devastating mortality since it was first identified in north america in 2002. in 2012, we conducted field trapping assays that tested the efficacy of purple prism and fluon-coated green multifunnel (lindgren funnel) traps. traps were baited with combinations of several lures that were previously shown to be attractive to a. planipennis: ...201425195441
influence of host age on critical fitness parameters of spathius galinae (hymenoptera: braconidae), a new parasitoid of the emerald ash borer (coleoptera: buprestidae).spathius galinae belokobylskij and strazenac (hymenoptera: braconidae) is a recently discovered gregarious idiobiont larval ectoparasitoid currently being evaluated for biological control against the invasive emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae) in the united states. to aid in the development of laboratory rearing protocols, we assessed the influence of various emerald ash borer stages on critical fitness parameters of s. galinae. we exposed gravid s. galina ...201425195418
biology, life history, and laboratory rearing of spathius galinae (hymenoptera: braconidae), a larval parasitoid of the invasive emerald ash borer (coleoptera: buprestidae).spathius galinae belokobylskij & strazanac is a recently described parasitoid of the emerald ash borer, agrilus planipennis fairmaire, in the russian far east, and is currently being considered for biocontrol introduction in the united states. using a. planipennis larvae reared with freshly cut ash (fraxinus spp.) sticks, we investigated the biology, life cycle, and rearing of s. galinae in the laboratory under normal rearing conditions (25 +/- 1 degrees c, 65 +/- 10% relative humidity, and a ph ...201425026651
male-produced pheromone of spathius agrili, a parasitoid introduced for the biological control of the invasive emerald ash borer, agrilus planipennis.the braconid wasp, spathius agrili, has been released in the u.s. as a biocontrol agent for the invasive emerald ash borer (coleoptera: buprestidae: agrilus planipennis), a destructive pest of ash trees (fraxinus spp.). we identified and synthesized seven male-specific volatile compounds. three of these, dodecanal, (4r,11e)-tetradecen-4-olide, and (z)-10-heptadecen-2-one, were the key behaviorally active components in flight tunnel bioassays. male specificity was demonstrated by gas chromatograp ...201222456948
preimaginal stages of the emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae): an invasive pest on ash trees (fraxinus).this study provides the most detailed description of the immature stages of agrilus planipennis fairmaire to date and illustrates suites of larval characters useful in distinguishing among agrilus curtis species and instars. immature stages of eight species of agrilus were examined and imaged using light and scanning electron microscopy. for a. planipennis all preimaginal stages (egg, instars i-iv, prepupa and pupa) were described. a combination of 14 character states were identified that serve ...201222438898
estimating the influence of population density and dispersal behavior on the ability to detect and monitor agrilus planipennis (coleoptera: buprestidae) populations.emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae), a phloem-feeding pest of ash (fraxinus spp.) trees native to asia, was first discovered in north america in 2002. since then, a. planipennis has been found in 15 states and two canadian provinces and has killed tens of millions of ash trees. understanding the probability of detecting and accurately delineating low density populations of a. planipennis is a key component of effective management strategies. here we approac ...201222420280
economic analysis of emerald ash borer (coleoptera: buprestidae) management options.emerald ash borer, agrilus planipennis (fairmaire) (coleoptera: buprestidae), plays a significant role in the health and extent of management of native north american ash species in urban forests. an economic analysis of management options was performed to aid decision makers in preparing for likely future infestations. separate ash tree population valuations were derived from the i-tree streets program and the council of tree and landscape appraisers (ctla) methodology. a relative economic anal ...201222420272
spatial and temporal distribution of trunk-injected (14) c-imidacloprid in fraxinus trees.since the discovery of agrilus planipennis fairmaire (emerald ash borer) in 2002, researchers have tested several methods of chemical control. soil drench or trunk injection products containing imidacloprid are commonly used to control adults. however, efficacy can be highly variable and may be due to uneven translocation of systemic insecticides. the purpose of this study was to determine whether sectored xylem anatomy might influence imidacloprid distribution in tree crowns.201222290795
effects of trap type, placement and ash distribution on emerald ash borer captures in a low density site.effective methods for early detection of newly established, low density emerald ash borer (agrilus planipennis fairmaire) infestations are critically needed in north america. we assessed adult a. planipennis captures on four types of traps in a 16-ha site in central michigan. the site was divided into 16 blocks, each comprised of four 50- by 50-m cells. green ash trees (fraxinus pennsylvanica marshall) were inventoried by diameter class and ash phloem area was estimated for each cell. one trap t ...201122251735
evidence for a volatile pheromone in agrilus planipennis fairmaire (coleoptera: buprestidae) that increases attraction to a host foliar volatile.analysis by gas chromatography/mass spectrometry (gc/ms) of volatiles from virgin female emerald ash borer, agrilus planipennis fairmaire confirmed the emission of (3z)-lactone [(3z)-dodecen-12-olide] but not its geometric isomer, (3e)-lactone [(3e)-dodecen-12-olide]. gas chromatographic/electroantennographic (gc/ead) analysis of synthetic (3z)-lactone, which contained 10% (3e)-lactone, showed a strong response of male and female antennae to both isomers. eag analysis with 0.01- to 100-μg dosage ...201122251692
detectability of the emerald ash borer (coleoptera: buprestidae) in asymptomatic urban trees by using branch samples.the emerald ash borer, agrilus planipennis fairmaire, is an exotic invasive insect causing extensive mortality to ash trees, fraxinus spp., in canada and the united states. detection of incipient populations of this pest is difficult because of its cryptic life stages and a multiyear time lag between initial attack and the appearance of signs or symptoms of infestation. we sampled branches from open-grown urban ash trees to develop a sample unit suitable for detecting low density a. planipennis ...201122251647
the biology and ecology of the emerald ash borer, agrilus planipennis, in china.the biology, ecology, and life cycle of the emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae), were studied using regular inspection in the forest and observations in the laboratory. results indicated that a. planipennis are mostly univoltine in tianjin, china. they overwintered individually as mature larvae in shallow chambers excavated in the outer sapwood. in late july, some full-grown larvae began to build overwintering chambers, and all larvae entered the sapwood fo ...201020879922
biology and life history of balcha indica, an ectoparasitoid attacking the emerald ash borer, agrilus planipennis, in north america.balcha indica mani and kaul (hymenoptera: eupelmidae) is a solitary ectoparasitoid attacking larvae, prepupae, and pupae of the emerald ash borer, agrilus planipennis fairmaire (hymenoptera: eupelmidae). its fecundity, oviposition rate, longevity, and development time were determined in the laboratory under standard rearing conditions (25 ± 2° c, 65 ± 10% relative humidity, and 14:10 l:d). adults lived a mean of 59 days with a maximum of 117 days. lifetime adult fecundity averaged 36 eggs with a ...201122233385
field-cage methodology for evaluating climatic suitability for introduced wood-borer parasitoids: preliminary results from the emerald ash borer system.field-cage methods were developed to evaluate the abilities of tetrastichus planipennisi yang (hymenoptera: eulophidae) and spathius agrili yang (hymenoptera: braconidae), biocontrol agents of agrilus planipennis fairmaire (coleoptera: buprestidae), to parasitize, develop and overwinter following three late-season releases at both a northern (michigan) and a southern (maryland) location within the current north american range of a. planipennis. in august, september and october of 2009, five youn ...201122233133
evaluation of agrilus planipennis (coleoptera: buprestidae) control provided by emamectin benzoate and two neonicotinoid insecticides, one and two seasons after treatment.effective methods are needed to protect ash trees (fraxinus spp.) from emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae), an invasive buprestid that has killed millions of north american ash (fraxinus spp.) trees. we randomly assigned 175 ash trees (11.5-48.1 cm in diameter) in 25 blocks located in three study sites in michigan to one of seven insecticide treatments in may 2007. treatments included 1) trunk-injected emamectin benzoate; 2) trunk-injected imidacloprid; 3) ...201122066190
interspecific proteomic comparisons reveal ash phloem genes potentially involved in constitutive resistance to the emerald ash borer.the emerald ash borer (agrilus planipennis) is an invasive wood-boring beetle that has killed millions of ash trees since its accidental introduction to north america. all north american ash species (fraxinus spp.) that emerald ash borer has encountered so far are susceptible, while an asian species, manchurian ash (f. mandshurica), which shares an evolutionary history with emerald ash borer, is resistant. phylogenetic evidence places north american black ash (f. nigra) and manchurian ash in the ...201121949771
feeding by emerald ash borer larvae induces systemic changes in black ash foliar chemistry.the exotic wood-boring pest, emerald ash borer (eab), agrilus planipennis fairmaire (coleoptera: buprestidae), has been threatening north american ash (fraxinus spp.) resources, this being recognized since its first detection in michigan, usa and ontario, canada in 2002. ash trees are killed by larval feeding in the cambial region, which results in disruption of photosynthate and nutrient translocation. in this study, changes in volatile and non-volatile foliar phytochemicals of potted 2-yr-old ...201121802697
efficacy of multifunnel traps for capturing emerald ash borer (coleoptera: buprestidae): effect of color, glue, and other trap coatings.tens of thousands of adhesive-coated purple prism traps are deployed annually in the united states to survey for the invasive emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae). a reusable, more user-friendly trap is desired by program managers, surveyors, and researchers. field assays were conducted in southeastern michigan to ascertain the feasibility of using nonsticky traps as survey and detection tools for emerald ash borer. three nonsticky trap designs, including mu ...201121735910
analytical study of azadirachtin and 3-tigloylazadirachtol residues in foliage and phloem of hardwood tree species by liquid chromatography-electrospray mass spectrometry.a rapid and sensitive lc-esi-ms method has been developed and validated for the quantitation of azadirachtin and 3-tigloylazadirachtol in deciduous tree matrices. the method involves automated extraction and simultaneous cleanup using an accelerated solvent technique with the matrix dispersed in solid phase over a layer of primary-secondary amine silica. the limits of quantification were 0.02 mg/kg for all matrices with the exception of norway maple foliage (0.05 mg/kg). validation at three leve ...201121726086
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