| expression of the oligomerization domain of the replication-associated protein (rep) of tomato leaf curl new delhi virus interferes with dna accumulation of heterologous geminiviruses. | the minimal dna binding domain of the replication-associated protein (rep) of tomato leaf curl new delhi virus was determined by electrophoretic mobility gel shift analysis and co-purification assays. dna binding activity maps to amino acids 1-160 (rep-(1-160)) of the rep protein and overlaps with the protein oligomerization domain. transient expression of rep protein (rep-(1-160)) was found to inhibit homologous viral dna accumulation by 70-86% in tobacco protoplasts and in nicotiana benthamian ... | 2001 | 11342533 |
| the nuclear shuttle protein of tomato leaf curl new delhi virus is a pathogenicity determinant. | the role of the movement protein (mp) and nuclear shuttle protein (nsp) in the pathogenicity of tomato leaf curl new delhi virus (tolcndv), a bipartite begomovirus, was studied. both genes were expressed in nicotiana benthamiana, nicotiana tabacum, and lycopersicon esculentum plants with the potato virus x (pvx) expression vector or by stable transformation of gene constructs under the control of the 35s promoter in n. tabacum. no phenotypic changes were observed in any of the three species when ... | 2005 | 15767443 |
| silencing of the av2 gene by antisense rna protects transgenic plants against a bipartite begomovirus. | whitefly-transmitted geminiviruses (genus begomovirus) are phytopathogens that cause heavy losses to crops worldwide. efforts to engineer resistance against these viruses are focused mainly on silencing of complementary-sense virus genes involved in virus replication. here we have targeted a virion-sense gene (av2) to develop resistance against tomato leaf curl new delhi virus, a bipartite begomovirus prevalent throughout the indian subcontinent. we show that tobacco plants transformed with an a ... | 2007 | 17239233 |
| a monopartite begomovirus-associated dna beta satellite substitutes for the dna b of a bipartite begomovirus to permit systemic infection. | dna beta is a circular single-stranded satellite dna which co-infects with certain monopartite helper begomoviruses to cause economically important diseases, such as cotton leaf curl disease (clcud). dna beta encodes a single protein, betac1. tomato leaf curl new delhi virus (tolcndv) is a bipartite begomovirus in which both dna a and dna b are required for systemic infection. inoculation of tomato plants with tolcndv dna a alone induced local but not systemic infection, whereas co-inoculation w ... | 2007 | 17872543 |
| the hypersensitive response to tomato leaf curl new delhi virus nuclear shuttle protein is inhibited by transcriptional activator protein. | the hypersensitive response (hr) is a common feature of plant disease resistance reactions and a type of programmed cell death (pcd). many pathogens are able to modulate pathways involved in cell death. in contrast to animal viruses, inhibitors of pcd activity have not been identified for plant-infecting viruses. previously, we have reported that the nuclear shuttle protein (nsp) of tomato leaf curl new delhi virus (tolcndv) induces an hr in nicotiana tabacum and lycopersicon esculentum plants w ... | 2007 | 17990965 |
| complete nucleotide sequence of a new isolate of tomato leaf curl new delhi virus infecting cucumber, bottle gourd and muskmelon in thailand. | | 2008 | 18193155 |
| supervirulent pseudorecombination and asymmetric synergism between genomic components of two distinct species of begomovirus associated with severe tomato leaf curl disease in india. | isolates of two distinct begomovirus species, the severe strain of the species tomato leaf curl new delhi virus (tomato leaf curl new delhi virus-[india:new delhi:severe:1992]; tolcndv-[in:nd:svr:92], bipartite) and the varanasi strain of the species tomato leaf curl gujarat virus (tomato leaf curl gujarat virus-[india:varanasi:2001]; tolcgv-[in:var:01], mono/bipartite) infect tomato (lycopersicon esculentum) and cause severe yield losses in northern india. this study investigated the infectivit ... | 2008 | 18272774 |
| molecular characterization of a distinct bipartite begomovirus species infecting tomato in india. | a distinct bipartite begomovirus was found associated with tomato plants showing yellowing, curling, and crumpling of the leaves, in a sub-temperate region in india. the complete dna-a and dna-b components were amplified through rolling circle amplification (rca) using phi-29 dna polymerase and characterized. the dna-a of the isolate was comprised of 2,756 nucleotides, encoding six open reading frames (orfs) and dna-b that of 2,725 nucleotides, encoding two orfs. genome organization of the isola ... | 2008 | 18792773 |
| nucleotide sequencing, whitefly transmission, and screening tomato for resistance against two newly described begomoviruses in bangladesh. | abstract the molecular diversity of tomato leaf curl viruses (tolcvs), from the two main tomato growing areas of jessore and joydebpur, bangladesh, was investigated. the viral dna was amplified from tomato plants exhibiting mild and severe symptoms by polymerase chain reaction, and the complete genomes of the tolcvs were sequenced. an isolate of the bipartite tomato leaf curl new delhi virus-severe (tolcndv-svr) was associated with the severe symptom phenotype from jessore (tolcndv-svr[jes]). a ... | 2005 | 18943559 |
| tomato leaf curl gujarat virus, a new begomovirus species causing a severe leaf curl disease of tomato in varanasi, india. | abstract the biological and molecular properties of tomato leaf curl gujarat virus from varanasi, india (tolcgv-[var]) were characterized. tolcgv-[var] could be transmitted by grafting and through whitefly transmission in a persistent manner. the full-length genome of dna-a and dna-b of tolcgv-[var] was cloned in puc18. sequence analysis revealed that dna-a (ay190290) is 2,757 bp and dna-b (ay190291) is 2,688 bp in length. tolcgv-[var] could infect and cause symptoms in tomato, pepper, nicotiana ... | 2003 | 18943612 |
| tomato leaf curl karnataka virus from bangalore, india, appears to be a recombinant begomovirus. | abstract the genome of tomato leaf curl virus (tolcv) from bangalore, india, a whitefly-transmitted geminivirus, was cloned (pind9) and sequenced. the circular dna of 2,759 nucleotides (u38239) is organized similarly to that of other begomoviruses with monopartite genomes. comparison of the nucleotide sequence of pind9 with other tomato-associated begomoviruses from india (tomato leaf curl bangalore virus [tolcbv, z48182]) and tomato leaf curl new delhi virus-severe (tolcndv-svr, u15015) showed ... | 2002 | 18944261 |
| genetic diversity and distribution of tomato-infecting begomoviruses in iran. | the incidence and severity of tomato leaf curl disease (tlcd) is increasing worldwide. here we assess the diversity and distribution within tomato producing areas of iran of begomoviruses that cause this disease. tomato with typical tlcd symptoms and asymptomatic weeds were collected in 2005 and 2006 and tested for the presence of begomovirus dna using polymerase chain reaction (pcr). analysis of cloned and sequenced pcr products revealed that both mono- and bipartite begomoviruses are associate ... | 2009 | 19112612 |
| generation and characterization of a scfv against recombinant coat protein of the geminivirus tomato leaf curl new delhi virus. | we report the establishment of a hybridoma cell line secreting the monoclonal antibody (mab) hav, which recognizes the coat (av1) protein of tomato leaf curl new delhi virus (tolcndv), a begomovirus. the cell line was obtained following immunization of mice with purified recombinant av1 fused to glutathione s-transferase (gst). a single-chain variable fragment (scfv-sav) was assembled from hybridoma cdna, but sequence analysis revealed a single nucleotide deletion causing a frame shift that resu ... | 2010 | 20107847 |
| molecular characterization of a new species of begomovirus and betasatellite causing leaf curl disease of tomato in india. | a new tomato-infecting begomovirus and cognate betasatellite were characterized from the gangetic plain of northern india. genome organization of this virus was found to be similar to those of other old world begomoviruses. the dna-a molecule (2752nt) shared maximum (85.8%) identity with tomato leaf curl laos virus-[laos] (tolclv-[la]; af195782) from laos and betasatellite molecule (1349nt) shared maximum (75.8%) identity with tomato leaf curl joydebpur betasatellite (tolcjob-[bd:gaz:05]; aj9662 ... | 2010 | 20540978 |
| tomato cultivar tolerant to tomato leaf curl new delhi virus infection induces virus-specific short interfering rna accumulation and defence-associated host gene expression. | tomato leaf curl new delhi virus (tolcndv) infection causes significant yield loss in tomato. the availability of a conventional tolerance source against this virus is limited in tomato. to understand the molecular mechanism of virus tolerance in tomato, the abundance of viral genomic replicative intermediate molecules and virus-directed short interfering rnas (sirnas) by the host plant in a naturally tolerant cultivar h-88-78-1 and a susceptible cultivar punjab chhuhara at different time points ... | 2010 | 20618710 |
| complete nucleotide sequences of a distinct bipartite begomovirus, bitter gourd yellow vein virus, infecting momordica charantia. | momordica charantia (cucurbitaceae), a vegetable crop commonly cultivated throughout pakistan, and begomoviruses, a serious threat to crop plants, are natives of tropical and subtropical regions of the world. leaf samples of m. charantia with yellow vein symptoms typical of begomovirus infections and samples from apparently healthy plants were collected from areas around lahore in 2004. full-length clones of a bipartite begomovirus were isolated from symptomatic samples. the complete nucleotide ... | 2010 | 20924621 |
| microrna profiling of tomato leaf curl new delhi virus (tolcndv) infected tomato leaves indicates that deregulation of mir159/319 and mir172 might be linked with leaf curl disease. | tomato leaf curl virus (tolcv), a constituent of the genus begomovirus, infects tomato and other plants with a hallmark disease symptom of upward leaf curling. since micrornas (mirs) are known to control plants developmental processes, we evaluated the roles of mirnas in tomato leaf curl new delhi virus (tolcndv) induced leaf curling. | 2010 | 20973960 |
| pepper leaf curl lahore virus requires the dna b component of tomato leaf curl new delhi virus to cause leaf curl symptoms. | begomoviruses are whitefly-transmitted geminiviruses with genomes that consist of either two components (known as dna a and dna b) or a single component (homologous to the dna a component of bipartite begomoviruses). monopartite begomoviruses are often associated with a symptom-modulating dna satellite (collectively known as betasatellites). both bipartite and monopartite begomoviruses with associated satellites have previously been identified in chillies showing leaf curl symptoms in pakistan. | 2010 | 21144019 |
| tomato leaf curl new delhi virus dna a component and cotton leaf curl multan betasatellite can cause mild transient symptoms in cotton. | | 2010 | 21175259 |
| a novel recombinant tomato-infecting begomovirus capable of transcomplementing heterologous dna-b components. | the genome of a tomato-infecting begomovirus from ranchi, india, was cloned, sequenced and analysed. the viral genome shared 88.3% sequence identity with an isolate belonging to the species tobacco curly shoot virus (tbcsv), and this virus should therefore be considered a member of a new species, tentatively named tomato leaf curl ranchi virus (tolcrnv). the dna-ß molecule, which had 74.5% sequence identity with tomato leaf curl bangladesh betasatellite (tolcbdb), is named tomato leaf curl ranch ... | 2011 | 21311922 |
| infectious clones of tomato leaf curl palampur virus with a defective dna b and their pseudo-recombination with tomato leaf curl new delhi virus. | abstract: background: tomato leaf curl palampur virus (tolcpmv) is a bipartite begomovirus which has been reported from india and iran but infectious clones have not been obtained. we have previously shown the association of zucchini yellow mosaic virus (zymv), a potyvirus, with severe leaf curl disease of muskmelon in pakistan. however, the severity of symptoms in the field and yield losses led us to believe that some other agent, such as a begomovirus, could be associated with the disease. res ... | 2011 | 21496256 |
| differential expression analyses of host genes involved in systemic infection of tomato leaf curl new delhi virus (tolcndv). | tomato leaf curl viruses (tolcv) infect tomato plants and eventually cause several phenotypic defects, notably in the leaves in the form of upward curling. the entry of virus triggers plants' basal defense responses which eventually introduce temporal changes in the transcriptome to evade the pathogen attack. in this study, we have identified about 20 tomato ests using subtractive hybridization that were induced in tomato leaves upon agro-infection with the constructs bearing the dimers of tomat ... | 2011 | 21600246 |
| molecular characterization and infectivity of a tomato leaf curl new delhi virus variant associated with newly emerging yellow mosaic disease of eggplant in india. | begomoviruses have emerged as serious problem for vegetable and fiber crops in the recent past, frequently in tropical and subtropical region of the world. the association of begomovirus with eggplant yellow mosaic disease is hitherto unknown apart from one report from thailand. a survey in nagpur, central india, in 2009-2010 showed severe incidence of eggplant yellow mosaic disease. here, we have identified and characterized a begomovirus responsible for the newly emerging yellow mosaic disease ... | 2011 | 21676270 |
| dynamics of defense-related components in two contrasting genotypes of tomato upon infection with tomato leaf curl new delhi virus. | tomato leaf curl virus (tolcv) disease is a serious threat for tomato cultivation in the tropics and subtropics. despite serious efforts no immune commercial varieties or f(1) hybrids are available till date. in this study, the interaction between solanum lycopersicum and tolcv was characterized on molecular and biochemical basis. rna silencing mediated by short interfering rna (sirna) and reactive oxygen species (ros) has been proposed as central components of plant adaptation to several stress ... | 2011 | 22161255 |
| reactions of nicotiana species to inoculation with monopartite and bipartite begomoviruses. | some nicotiana species are widely used as experimental hosts for plant viruses. nicotiana species differ in ploidy levels, chromosome numbers and have diverse geographical origins. thus, these species are useful model systems to investigate virus-host interactions, co-evolution of pathogens and hosts and the effects of ploidy level on virus resistance/susceptibility. | 2011 | 22011413 |
| tomato leaf curl new delhi virus is associated with pumpkin leaf curl: a new disease in northern india. | during 2006, pumpkin leaf curl-a new disease was observed in the experimental field at indian agricultural research institute. the disease was characterized by upward leaf curl with chlorotic patches and stunting of plant. polymerase chain reaction (pcr) with coat protein specific primers to tomato leaf curl new delhi virus (tolcndv) indicated association of a begomovirus with the disease. the sequence comparison and phylogenetic analysis of the complete dna genome further revealed the identity ... | 2011 | 23730002 |
| molecular characterization of tomato leaf curl palampur virus and pepper leaf curl betasatellite naturally infecting pumpkin (cucurbita moschata) in india. | pumpkin cultivation in india is affected by severe incidence of a yellow vein mosaic disease. tomato leaf curl new delhi virus and squash leaf curl china virus are known to be associated with this disease in india. we were able to identify a third begomovirus-tomato leaf curl palampur virus (tolcpmv), from pumpkin showing typical symptoms of the disease at varanasi based on the sequence of complete dna-a genome of the virus. the complete dna-a sequence of the virus shared more than 99% sequence ... | 2011 | 23637491 |
| transgenic tomato plants expressing artificial micrornas for silencing the pre-coat and coat proteins of a begomovirus, tomato leaf curl new delhi virus, show tolerance to virus infection. | designing artificial micrornas (amirs) targeting the genes responsible for viral replication, transmission and symptom development after viral infection offers a promising strategy to contain the multiplication and spread of geminiviruses in host plants. here, we report the design of two amirs targeting the middle region of the av1 (coat protein) transcript (amir-av1-3) and the overlapping region of the av1 and av2 (pre-coat protein) transcripts (amir-av1-1) of a model geminivirus, tomato leaf c ... | 2012 | 23276684 |
| association of tomato leaf curl new delhi virus dna-b with bhendi yellow vein mosaic virus in okra showing yellow vein mosaic disease symptoms. | okra samples showing yellow vein mosaic, vein twisting and bushy appearance were collected from different locations of india during the surveys conducted between years 2005-2009. the dot blot and pcr detection revealed that 75.14% of the samples were associated with monopartite begomovirus and remaining samples with bipartite virus. whitefly transmission was established for three samples representing widely separated geographical locations which are negative to betasatellites and associated with ... | 2015 | 26104329 |
| synergistic interaction among begomoviruses leads to the suppression of host defense-related gene expression and breakdown of resistance in chilli. | chilli (capsicum sp.) is one of the economically important spice and vegetable crops grown in india and suffers great losses due to the infection of begomoviruses. conventional breeding approaches have resulted in development of a few cultivars of chilli resistant to begomoviruses. a severe leaf curl disease was observed on one such resistant chilli cultivar (capsicum annuum cv. kalyanpur chanchal) grown in the experimental field of the jawaharlal nehru university, new delhi. four different vira ... | 2016 | 26780359 |
| rnai mediated broad-spectrum transgenic resistance in nicotiana benthamiana to chilli-infecting begomoviruses. | two rnai constructs were designed targeting chilli-infecting begomoviruses and associated betasatellites. broad-spectrum resistance was achieved against multiple begomoviruses associated with leaf curl disease of chillies in india. chilli leaf curl disease (chilcd) caused by begomoviruses (family: geminiviridae) has emerged as one of the most devastating viral diseases of chilli, especially in the indian sub-continent. the severity of disease incidence is expanding at an alarming rate due to the ... | 2015 | 25916177 |
| spatial and temporal diversity of begomoviral complexes in papayas with leaf curl disease. | old world, monopartite begomoviruses associated with satellite dna β were observed in papaya showing symptoms of leaf curl disease sampled randomly over five years from within a radius of 250 km in north-central india. three groups of dna a sequences were evident. one group resembled chili leaf curl virus infecting tomatoes (chilcuv). another group resembled tomato leaf curl new delhi virus (tolcundv). the third group was novel (tentatively named papaya leaf crumple virus, palcrv), with less tha ... | 2012 | 22437254 |
| frequent occurrence of tomato leaf curl new delhi virus in cotton leaf curl disease affected cotton in pakistan. | cotton leaf curl disease (clcud) is the major biotic constraint to cotton production on the indian subcontinent, and is caused by monopartite begomoviruses accompanied by a specific dna satellite, cotton leaf curl multan betasatellite (clcumb). since the breakdown of resistance against clcud in 2001/2002, only one virus, the "burewala" strain of cotton leaf curl kokhran virus (clcukov-bur), and a recombinant form of clcumb have consistently been identified in cotton across the major cotton growi ... | 2016 | 27213535 |
| biology and interactions of two distinct monopartite begomoviruses and betasatellites associated with radish leaf curl disease in india. | emerging whitefly transmitted begomoviruses are major pathogens of vegetable and fibre crops throughout the world, particularly in tropical and sub-tropical regions. mutation, pseudorecombination and recombination are driving forces for the emergence and evolution of new crop-infecting begomoviruses. leaf curl disease of field grown radish plants was noticed in varanasi and pataudi region of northern india. we have identified and characterized two distinct monopartite begomoviruses and associate ... | 2012 | 22339942 |
| molecular characterization of a distinct bipartite begomovirus species infecting ivy gourd (coccinia grandis l.) in tamil nadu, india. | a distinct bipartite begomovirus was found to be associated with the mosaic disease on ivy gourd (coccinia grandis l.) in tamil nadu, india. the complete dna a and dna b components were cloned by rolling circle amplification. genome organization of this virus is found to be typical of old world bipartite begomovirus. the association of betasatellite component with this virus is absent. the closest nucleotide identity of 73.4 % was seen with the loofa yellow mosaic virus (lymv-[vn]-af509739) sugg ... | 2016 | 26739457 |
| a bemisia tabaci midgut protein interacts with begomoviruses and plays a role in virus transmission. | begomoviruses are a major group of plant viruses, transmitted exclusively by bemisia tabaci (gennadius) in a persistent circulative non-propagative manner. the information regarding molecular and cellular basis underlying begomovirus - whitefly interaction is very scarce. evidences have suggested that the insect gut possesses some crucial protein receptors that allow specific entry of virus into the insect haemolymph. we have performed yeast two hybrid gut cdna expression library screening again ... | 2016 | 26488331 |
| chlorotic curly stunt: a severe begomovirus disease of bottle gourd in northern india. | bottle gourd (lagenaria siceraria) an important vegetable crop in india was observed to be affected by a chlorotic curly stunt disease (ccsd) during 2003-2006 in the vegetable growing areas of delhi and adjoining state of haryana. the affected plants are severely stunted and bear very small chlorotic and mildly curled leaves. incidence of the disease varied from 4.7 to 36%. the disease could be easily transmitted by whitefly, bemisia tabaci but not by sap. the causal virus was found to be a bego ... | 2010 | 23637479 |
| development of plants resistant to tomato geminiviruses using artificial trans-acting small interfering rna. | rna interference (rnai), a conserved rna-mediated gene regulatory mechanism in eukaryotes, plays an important role in plant growth and development, and as an antiviral defence system in plants. as a counter-strategy, plant viruses encode rnai suppressors to suppress the rnai pathways and consequently down-regulate plant defence. in geminiviruses, the proteins ac2, ac4 and av2 are known to act as rnai suppressors. in this study, we have designed a gene silencing vector using the features of trans ... | 2015 | 25512230 |
| an insight into differentially regulated genes in resistant and susceptible genotypes of potato in response to tomato leaf curl new delhi virus-[potato] infection. | apical leaf curl disease, caused by tomato leaf curl new delhi virus-[potato] (tolcndv-[potato]) is one of the most important viral diseases of potato in india. genetic resistance source for tolcndv in potato is not identified so far. however, the cultivar kufri bahar is known to show lowest seed degeneration even under high vector levels. hence, microarray analysis was performed to identify differentially regulated genes during tolcndv-[potato] infection in a resistant (kufri bahar) and a susce ... | 2017 | 28115198 |
| a novel strain of tomato leaf curl new delhi virus has spread to the mediterranean basin. | tomato leaf curl new delhi virus (tolcndv) is a whitefly-transmitted bipartite begomovirus (genus begomovirus, family geminiviridae) that causes damage to multiple cultivated plant species mainly belonging to the solanaceae and cucurbitaceae families. tolcndv was limited to asian countries until 2012, when it was first reported in spain, causing severe epidemics in cucurbit crops. here, we show that a genetically-uniform tolcndv population is present in spain, compatible with a recent introducti ... | 2016 | 27834936 |
| molecular characterization of a first begomovirus associated with lentil (lens culinaris) from india. | a disease of lentil with symptoms of distortion, mottling and chlorosis in the leaves, shortening of internodes and excessive branching was noticed in lentil at kanpur, india, during 2012-2014. results of polymerase chain reaction and reverse transcriptase polymerase chain reaction employed to detect suspected rna and dna viruses indicated involvement of a geminivirus, which was further characterized by sequencing of full genome amplified by rolling circle amplification. analysis of full length ... | 2017 | 27640431 |
| tomato leaf curl new delhi virus: a widespread bipartite begomovirus in the territory of monopartite begomoviruses. | tomato leaf curl new delhi virus (tolcndv) is an exceptional old world bipartite begomovirus. on the indian subcontinent, a region in which monopartite dna satellite-associated begomoviruses with mostly narrow geographical ranges predominate, it is widespread, with a geographical range also including the far east, middle east, north africa and europe. the success of tolcndv probably derives from its broad host range and highly flexible genomic configuration: its dna-a component is capable of pro ... | 2016 | 27553982 |
| natural occurrence of tomato leaf curl new delhi virus in iranian cucurbit crops. | the main areas for field-grown vegetable production in iran were surveyed during the years of 2012-2014 to determine the occurrence of begomoviruses infecting these crops. a total of 787 leaf samples were collected from vegetables and some other host plants showing virus-like symptoms and tested by an enzyme-linked immunosorbent assay (elisa) using polyclonal antibodies produced against tomato yellow leaf curl virus (tylcv). according to the elisa results, 81 samples (10.3%) positively reacted w ... | 2016 | 27298595 |
| tomato 26s proteasome subunit rpt4a regulates tolcndv transcription and activates hypersensitive response in tomato. | involvement of 26s proteasomal subunits in plant pathogen-interactions, and the roles of each subunit in independently modulating the activity of many intra- and inter-cellular regulators controlling physiological and defense responses of a plant were well reported. in this regard, we aimed to functionally characterize a solanum lycopersicum 26s proteasomal subunit rpt4a (slrpt4) gene, which was differentially expressed after tomato leaf curl new delhi virus (tolcndv) infection in tolerant culti ... | 2016 | 27252084 |
| mechanism of regulation of tomato trn1 gene expression in late infection with tomato leaf curl new delhi virus (tolcndv). | tomato leaf curl disease caused by geminiviruses is manifested by curling and puckering of leaves and thickening of veins, resembling developmental defects. this is probably due to the long-term altered regulation of expression of development related gene(s). our results show that in the infected leaves the transcript level of tornado1 (sltrn1), a gene important for cell expansion and vein formation, increased significantly. sltrn1 is transcribed from two start sites. the preferential usage of o ... | 2015 | 26706073 |
| differential response of diverse solanaceous hosts to tomato leaf curl new delhi virus infection indicates coordinated action of nbs-lrr and rnai-mediated host defense. | tomato leaf curl new delhi virus (tolcndv) is a bipartite begomovirus (family geminiviridae) that infects a wide range of plants. tolcndv has emerged as an important pathogen and a serious threat to tomato production in india. a comparative and molecular analysis of tolcndv pathogenesis was performed on diverse solanaceous hosts (capsicum annuum, nicotiana benthamiana, n. tabacum, and solanum lycopersicum). n. benthamiana was found to be the most susceptible host, whereas c. annuum showed resist ... | 2015 | 25894479 |
| prediction and characterization of tomato leaf curl new delhi virus (tolcndv) responsive novel micrornas in solanum lycopersicum. | tomato leaf curl new delhi virus (tolcndv) infects tomato (solanum lycopersicum) plants and causes severe crop losses. as the micrornas (mirnas) are deregulated during stressful events, such as biotic stress, we wanted to study the effect of tolcndv infection on tomato mirnas. we constructed two libraries, isolating small rnas (srnas) from healthy (ht) and tolcndv infected (it) tomato leaves, and sequenced the library-specific srnas using the next generation sequencing (ngs) approach. these data ... | 2015 | 25218481 |
| molecular detection and partial characterization of a begomovirus causing leaf curl disease of potato in sub-himalayan west bengal, india. | the characteristic disease symptoms of apical leaf curl, crinkled leaves and conspicuous mosaic were observed in potato plants grown in areas of coochbehar, one of the distinct agroclimatic zones of sub-himalayan west bengal. whitefly (bemisia tabaci) population was also observed in and around the infected plants. the characteristic disease symptoms and presence of whitefly (bemisia tabaci) population indicated the possibility of begomovirus infection. total dna was extracted from infected sampl ... | 2014 | 24813019 |
| molecular evidence for occurrence of tomato leaf curl new delhi virus in ash gourd (benincasa hispida) germplasm showing a severe yellow stunt disease in india. | an evaluation of 70 accessions of ash gourd germplasm grown at national bureau of plant genetic resources, new delhi, india during kharif season (2010) showed natural occurrence of a yellow stunt disease in three accessions (ic554690, ic036330 and pusa ujjwal). a set of begomovirus specific primers used in pcr gave expected amplicon from all the symptomatic plants; however no betasatellite was detected. complete genome of the begomovirus (dna-a and dna-b), amplified through rolling circle amplif ... | 2013 | 24426262 |
| uniplex and duplex pcr detection of geminivirus associated with potato apical leaf curl disease in india. | apical leaf curl disease has emerged as a new disease in potato during the last decade in india due to a change in planting date and an increased whitefly population. its incidence is on the rise threatening the cultivation of potato across the country. hence, a pcr assay was developed for the detection of tomato leaf curl new delhi virus-potato (tolcndv-potato) which is the causal agent of apical leaf curl disease in potato. primers specific to the coat protein (av1) and replicase (ac1) gene re ... | 2013 | 23711886 |
| asymmetric synergism and heteroencapsidation between two bipartite begomoviruses, tomato leaf curl new delhi virus and tomato leaf curl palampur virus. | | 2013 | 23578824 |
| infection of tomato leaf curl new delhi virus (tolcndv), a bipartite begomovirus with betasatellites, results in enhanced level of helper virus components and antagonistic interaction between dna b and betasatellites. | tomato leaf curl new delhi virus (tolcndv) (geminiviridae) is an important pathogen that severely affects tomato production. an extensive survey was carried out during 2003-2010 to study the diversity of begomoviruses found in tomato, potato, and cucurbits that showed symptoms of leaf puckering, distortion, curling, vein clearing, and yellow mosaic in various fields in different regions of india. ten begomovirus isolates were cloned from infected samples and identified as belonging to the specie ... | 2013 | 23306645 |
| in vivo expression and binding activity of scfv-rwav, which recognizes the coat protein of tomato leaf curl new delhi virus (family geminiviridae). | recombinant antibodies expressed in plants have the potential to interrupt virus infections by blocking essential stages of the infection cycle. here, we show that the expression of a recombinant single-chain variable fragment (scfv) that recognizes the coat protein of tomato leaf curl new delhi virus (tolcndv) in vitro can also bind to a recombinant coat protein in vivo in the reducing environment of the plant cytosol. the scfv and its target were both expressed as fluorescent protein fusions, ... | 2012 | 22491815 |
| role of betasatellite in the pathogenesis of a bipartite begomovirus affecting tomato in india. | betasatellites are commonly associated with tomato leaf curl disease caused by begomoviruses in india. this study demonstrates the role of a betasatellite in the pathogenesis of tomato leaf curl new delhi virus (tolcndv), a bipartite begomovirus affecting tomato in india. for infection, accumulation, systemic movement and disease induction by tolcndv, co-infection by the associated betasatellite was not essential, as the dna a alone of tolcndv could infect tomato and nicotiana benthamiana and in ... | 2012 | 22418785 |
| development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against tomato yellow leaf curl thailand virus. | tomato yellow leaf curl thailand virus, tylcthv, is a begomovirus that causes severe losses of tomato crops in thailand as well as several countries in southeast and east asia. the development of monoclonal antibodies (mabs) and serological methods for detecting tylcthv is essential for epidemiological studies and screening for virus-resistant cultivars. | 2017 | 28558726 |
| survey, symptomatology, transmission, host range and characterization of begomovirus associated with yellow mosaic disease of ridge gourd in southern india. | ridge gourd is an important vegetable crop and is affected by several biotic and abiotic factors. among the different biotic factors, ridge gourd yellow mosaic disease (rgymd) is new emerging threat for the production of ridge gourd. the incidence of the rgymd varied from 30 to 100% in southern india with highest disease incidence of 100% observed in belagavi district of karnataka state. the infected plants showed chlorosis, mosaic, cupping of leaves, blistering, reduction in leaf size and stunt ... | 2017 | 28770240 |
| resistance to tomato leaf curl new delhi virus in melon is controlled by a major qtl located in chromosome 11. | identification of three genomic regions and underlying candidate genes controlling the high level of resistance to tolcndv derived from a wild melon. snp markers appropriated for mas management of resistance. tomato leaf curl new delhi virus (tolcndv) is a bipartite begomovirus that severely affects melon crop (cucumis melo) in the main production areas of spain since 2012. in this work, we evaluated the degree of resistance of four accessions (two belonging to the subsp. agrestis var. momordica ... | 2017 | 28710536 |