| first report of the complete sequence of sida golden yellow vein virus from jamaica. | begomoviruses are phytopathogens that threaten food security [18]. sida spp. are ubiquitous weed species found in jamaica. sida samples were collected island-wide, dna was extracted via a modified dellaporta method, and the viral genome was amplified using degenerate and sequence-specific primers [2, 11]. the amplicons were cloned and sequenced. sequence analysis revealed that a dna-a molecule isolated from a plant in liguanea, st. andrew, was 90.9% similar to sida golden yellow vein virus-[unit ... | 2011 | 21625977 |
| mixed infection of sida jamaicensis in jamaica reveals the presence of three recombinant begomovirus dna a components. | begomoviruses impose serious constraints on agriculture throughout the temperate, tropical and subtropical regions. previously, we characterised a sida golden yellow vein virus isolate, sigyvv-[jm:lig2:08] (hq009519-20) from a symptomatic sida jamaicensis plant. with the aim of establishing whether it was hosting a mixed infection that could facilitate recombination, pcr-rflp was done on dna extracted from this plant, and the results suggested the presence of two additional genetically distinct ... | 2014 | 24687859 |