| preliminary identification and coat protein gene phylogenetic relationships of begomoviruses associated with native flora and cultivated plants from the yucatan peninsula of mexico. | a number of native and cultivated eudicots in the yucatan peninsula of mexico (ypm) exhibit symptoms associated with virus infection. symptomatic leaves were collected and assessed for begomoviral detection using polymerase chain reaction (pcr), and universal primers that amplify a fragment of the coat protein gene (core cp). begomovirus were detected in nine native and seven cultivated species, representing seven eudicot families. dna extracts from the 16 hosts were used for pcr amplification a ... | 2007 | 17682933 |
| functional analysis of bipartite begomovirus coat protein promoter sequences. | we demonstrate that the al2 gene of cabbage leaf curl virus (calcuv) activates the cp promoter in mesophyll and acts to derepress the promoter in vascular tissue, similar to that observed for tomato golden mosaic virus (tgmv). binding studies indicate that sequences mediating repression and activation of the tgmv and calcuv cp promoter specifically bind different nuclear factors common to nicotiana benthamiana, spinach and tomato. however, chromatin immunoprecipitation demonstrates that tgmv al2 ... | 2008 | 18430450 |
| symptom remission and specific resistance of pepper plants after infection by pepper golden mosaic virus. | abstract pepper golden mosaic virus (pepgmv) is an important begomovirus infecting solanaceous crops in mexico and central america. under controlled conditions for growth and inoculation with a low-pressure biolistic device, pepgmv-infected pepper plants consistently showed symptom remission or host recovery 12 to 15 days postinoculation (dpi). inoculated plants initially developed the characteristic pepgmv symptoms; however, newer leaves presented a significant decrease or disappearance of symp ... | 2007 | 18942936 |
| genetic and phenotypic variation of the pepper golden mosaic virus complex. | abstract three isolates of the bipartite begomovirus pepper golden mosaic virus (pepgmv) were characterized for genomic and biological properties. the complete nucleotide sequences of the dna-a and dna-b components were determined from infectious clones of pepgmv-serrano (pepgmv-ser), pepgmv-mosaic (pepgmv-mo), and pepgmv-distortion (pepgmv-d). nucleotide sequence identity among pepgmv components ranged from 91 to 96% for dna-a and from 84 to 99% for dna-b, with each pepgmv component most closel ... | 2005 | 18943475 |
| interactions between geminiviruses in a naturally occurring mixture: pepper huasteco virus and pepper golden mosaic virus. | abstract pepper huasteco virus (phv) and pepper golden mosaic virus (pepgmv) are found in mixtures in many horticultural crops in mexico. this combination constitutes an interesting, naturally occurring model system to study several aspects of virus-virus interactions. possible interactions between phv and pepgmv were studied at four levels: symptom expression, gene expression, replication, and movement. in terms of symptom expression, the interaction was shown to be host-dependent because antag ... | 2003 | 18944336 |
| rna silencing against geminivirus: complementary action of posttranscriptional gene silencing and transcriptional gene silencing in host recovery. | rna silencing in plants is a natural defense system mechanism against invading nucleic acids such as viruses. geminiviruses, a family of plant viruses characterized by a circular, single-stranded dna genome, are thought to be both inducers and targets of rna silencing. some natural geminivirus-host interactions lead to symptom remission or host recovery, a process commonly associated with rna silencing-mediated defense. pepper golden mosaic virus (pepgmv)-infected pepper plants show a recovery p ... | 2009 | 19019951 |
| identification of begomoviruses infecting crops and weeds in belize. | plants including pepper, red kidney bean, squash, string bean and tomato, as well as weeds with viral symptoms were collected from five districts in belize over a three year period with the aim of determining the diversity of the begomoviruses present. sixty five percent of the samples screened via dna hybridization produced signals indicative of begomovirus infection. subsequent pcr amplifications and nucleotide sequence analyses revealed the presence of four begomoviruses in belize. pepper gol ... | 2008 | 20596296 |
| characterization of geminivirus resistance in an accession of capsicum chinense jacq. | pepper golden mosaic virus (pepgmv) and pepper huasteco yellow vein virus (phyvv), members of the geminiviridae family, are important pathogens of pepper (capsicum annuum l.) and other solanaceous crops. accession bg-3821 of c. chinense jacq. was reported earlier as resistant to mixed infection with pepgmv and phyvv. in this work, we characterized the geminivirus resistance trait present in bg-3821. segregation analysis suggested that resistance depends on two genes. our data showed that pepgmv ... | 2011 | 20923365 |
| a germin-like protein gene (cchglp) of capsicum chinense jacq. is induced during incompatible interactions and displays mn-superoxide dismutase activity. | a germin-like gene (cchglp) cloned from geminivirus-resistant pepper (capsicum chinense jacq. line bg-3821) was characterized and the enzymatic activity of the expressed protein analyzed. the predicted protein consists of 203 amino acids, similar to other germin-like proteins. a highly conserved cupin domain and typical germin boxes, one of them containing three histidines and one glutamate, are also present in cchglp. a signal peptide was predicted in the first 18 n-terminal amino acids, as wel ... | 2011 | 22174599 |
| transcriptome analysis of symptomatic and recovered leaves of geminivirus-infected pepper (capsicum annuum). | geminiviruses are a large and important family of plant viruses that infect a wide range of crops throughout the world. the begomovirus genus contains species that are transmitted by whiteflies and are distributed worldwide causing disease on an array of horticultural crops. symptom remission, in which newly developed leaves of systemically infected plants exhibit a reduction in symptom severity (recovery), has been observed on pepper (capsicum annuum) plants infected with pepper golden mosaic v ... | 2012 | 23185982 |
| colonization by phloem-feeding herbivore overrides effects of plant virus on amino acid composition in phloem of chili plants. | the 'adaptive host manipulation' hypothesis predicts that parasites can enhance their transmission rates via manipulation of their host's phenotype. for example, many plant pathogens alter the nutritional quality of their host for herbivores that serve as their vectors. however, herbivores, including non-vectors, might cause additional alterations in the plant phenotype. here, we studied changes in the amino acid (aa) content in the phloem of chilli (capsicum annuum) plants infected with pepper ... | 2016 | 27558811 |
| two populations of viral minichromosomes are present in a geminivirus-infected plant showing symptom remission (recovery). | geminiviruses are important plant pathogens characterized by circular, single-stranded dna (ssdna) genomes. however, in the nuclei of infected cells, viral double-stranded dna (dsdna) associates with host histones to form a minichromosome. in phloem-limited geminiviruses, the characterization of viral minichromosomes is hindered by the low concentration of recovered complexes due to the small number of infected cells. nevertheless, geminiviruses are both inducers and targets of the host posttran ... | 2016 | 26792752 |
| the effect of ecosystem biodiversity on virus genetic diversity depends on virus species: a study of chiltepin-infecting begomoviruses in mexico. | current declines in biodiversity put at risk ecosystem services that are fundamental for human welfare. increasing evidence indicates that one such service is the ability to reduce virus emergence. it has been proposed that the reduction of virus emergence occurs at two levels: through a reduction of virus prevalence/transmission and, as a result of these epidemiological changes, through a limitation of virus genetic diversity. although the former mechanism has been studied in a few host-virus i ... | 2015 | 27774278 |
| microarray analysis of tomato plants exposed to the nonviruliferous or viruliferous whitefly vector harboring pepper golden mosaic virus. | plants are routinely exposed to biotic and abiotic stresses to which they have evolved by synthesizing constitutive and induced defense compounds. induced defense compounds are usually made, initially, at low levels; however, following further stimulation by specific kinds of biotic and abiotic stresses, they can be synthesized in relatively large amounts to abate the particular stress. cdna microarray hybridization was used to identify an array of genes that were differentially expressed in tom ... | 2014 | 25525099 |
| occurrence of squash yellow mild mottle virus and pepper golden mosaic virus in potential new hosts in costa rica. | leaf samples of solanum lycopersicum, capsicum annuum, cucurbita moschata, cucurbita pepo, sechium edule and erythrina spp. were collected. all samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. a sequence of ∼1,100 bp was obtained from the genomic component dna-a of 14 samples. in addition, one sequence of ∼580 bp corresponding to the coat protein (av1) was obtained from a chayote (s. edule) leaf sample. the presence of squash yellow mild mottle viru ... | 2013 | 25288955 |
| benzothiadiazole (bth) induces resistance to pepper golden mosaic virus (pepgmv) in pepper (capsicum annuum l.). | pepper is an economically important crop in many countries around the world but it is susceptible to many diseases. in mexico, diseases caused by bipartite begomoviruses have emerged as important problems in pepper. several control strategies have been explored wiht little success; most of them are based on the avoidance of virus transmission and the breeding for resistance. abiotic inducers can act at various points in the signaling pathways involved in disease resistance, providing long-lastin ... | 2013 | 24510135 |
| effects and effectiveness of two rnai constructs for resistance to pepper golden mosaic virus in nicotiana benthamiana plants. | tochlpv and pepgmv are begomoviruses that have adapted to a wide host range and are able to cause major diseases in agronomic crops. we analyzed the efficacy of induced resistance to pepgmv in nicotiana benthamiana plants with two constructs: one construct with homologous sequences derived from pepgmv, and the other construct with heterologous sequences derived from tochlpv. plants protected with the heterologous construct showed an efficacy to decrease the severity of symptoms of 45%, while pla ... | 2013 | 24287597 |
| pepgmv rep-protein expression in mammalian cells. | the geminiviruses genome is a small, single strand dna that replicates in the plant cell nucleus. analogous to animal dna viruses, geminiviruses depend on the host replication machinery to amplify their genomes and only supply the factors required to initiate their replication. consequently, geminiviruses remove the cell-cycle arrest and induce the host replication machinery using an endocycle process. they encode proteins, such as the conserved replication-associated proteins (rep) that interac ... | 2012 | 23170183 |