| alkanindiges illinoisensis gen. nov., sp. nov., an obligately hydrocarbonoclastic, aerobic squalane-degrading bacterium isolated from oilfield soils. | an alkane-degrading bacterium, designated gti mvab hex1(t), was isolated from chronically crude oil-contaminated soil from an oilfield in southern illinois. the isolate grew very weakly or not at all in minimal or rich media without hydrocarbons. straight-chain aliphatic hydrocarbons, such as hexadecane and heptadecane, greatly stimulated growth; shorter-chain (</=c(15)) hydrocarbons did not (with decane as the sole exception). growth was also greatly enhanced by the branched aliphatic hydrocarb ... | 2003 | 13130023 |
| alkanindiges hongkongensis sp. nov. a novel alkanindiges species isolated from a patient with parotid abscess. | a bacterium was isolated from the abscess pus of a 72-year-old patient with warthin's tumor and parotid abscess. the cells were aerobic, non-motile, gram-negative but difficult to be destained, non-sporulating, coccobacillus. the bacterium grew poorly on sheep blood agar and macconkey agar as non-hemolytic colonies of 0.5 mm in diameter after 24h of incubation at 37 degrees c in ambient air. growth was enhanced by tween 80. it produces catalase but not cytochrome oxidase. sequencing of the clone ... | 2005 | 15997704 |
| analysis of 525 samples to determine the usefulness of pcr amplification and sequencing of the 16s rrna gene for diagnosis of bone and joint infections. | the 16s rrna gene pcr in the diagnosis of bone and joint infections has not been systematically tested. five hundred twenty-five bone and joint samples collected from 525 patients were cultured and submitted to 16s rrna gene pcr detection of bacteria in parallel. the amplicons with mixed sequences were also cloned. when discordant results were observed, culture and pcr were performed once again. bacteria were detected in 139 of 525 samples. culture and 16s rrna gene pcr yielded identical documen ... | 2006 | 16517890 |
| populations related to alkanindiges, a novel genus containing obligate alkane degraders, are implicated in biological foaming in activated sludge systems. | activated sludge mixed liquor and biological foam samples were collected from five full-scale municipal wastewater treatment plants in illinois, all of which were exhibiting biological foaming at the time of sampling. oligonucleotide probe hybridization consistently measured higher levels of gammaproteobacteria rrna in the foam as compared with the mixed liquor for all treatment plants analysed. cloning and sequencing of 16s rrna gene amplicons led to the identification of populations which were ... | 2007 | 17635538 |
| perlucidibaca piscinae gen. nov., sp. nov., a freshwater bacterium belonging to the family moraxellaceae. | a freshwater bacterium, designated imcc1704(t), was isolated from a eutrophic pond. the strain was gram-negative, oxidase-positive, catalase-negative, chemoheterotrophic and facultatively aerobic with cells that were motile rods with a single polar flagellum. based on 16s rrna gene sequence similarity analyses, the novel strain was most closely related to the genera alkanindiges (91.7%), acinetobacter (89.0-91.2%), moraxella (87.9-90.1%), psychrobacter (87.2-89.5%) and enhydrobacter (87.8%). phy ... | 2008 | 18175691 |
| petroleum hydrocarbon biodegradation under seasonal freeze-thaw soil temperature regimes in contaminated soils from a sub-arctic site. | several studies have shown that biostimulation in ex situ systems such as landfarms and biopiles can facilitate remediation of petroleum hydrocarbon contaminated soils at sub-arctic sites during summers when temperatures are above freezing. in this study, we examine the biodegradation of semivolatile (f2: c10-c16) and nonvolatile (f3: c16-c34) petroleum hydrocarbons and microbial respiration and population dynamics at post- and presummer temperatures ranging from -5 to 14 °c. the studies were co ... | 2010 | 21194195 |