| use of the inno-lipa-mycobacteria assay (version 2) for identification of mycobacterium avium-mycobacterium intracellulare-mycobacterium scrofulaceum complex isolates. | using inno-lipa-mycobacteria (lipav1; innogenetics) and the accuprobe (gen-probe inc./biomérieux) techniques, 35 mycobacterium avium-mycobacterium intracellulare-mycobacterium scrofulaceum (mac/mais) complex strains were identified between january 2000 and december 2002. thirty-four of 35 isolates were positive only for the mais complex probe by lipav1 and were further analyzed by inno-lipa-mycobacteria version 2 (lipav2), hsp65 pcr restriction pattern analysis (pra), and ribosomal internal tran ... | 2005 | 15956365 |
| first detection of mycobacteria in african rodents and insectivores, using stratified pool screening. | with the rising number of patients with human immunodeficiency virus (hiv)/aids in developing countries, the control of mycobacteria is of growing importance. previous studies have shown that rodents and insectivores are carriers of mycobacteria. however, it is not clear how widespread mycobacteria are in these animals and what their role is in spreading them. therefore, the prevalence of mycobacteria in rodents and insectivores was studied in and around morogoro, tanzania. live rodents were tra ... | 2008 | 18065608 |
| occurrence and clinical relevance of mycobacterium chimaera sp. nov., germany. | retrospective molecular genetic analysis of 166 mycobacterium intracellulare isolates showed that 143 (86%) strains could be assigned to mycobacterium chimaera sp. nov. of 97 patients from whom m. chimaera sp. nov. was isolated, only 3.3% exhibited mycobacterial lung disease, whereas all m. intracellulare isolates caused severe pulmonary infections. | 2008 | 18760016 |
| molecular identification of mycobacterium chimaera as a cause of infection in a patient with chronic obstructive pulmonary disease. | this report describes a case of mycobacterium chimaera infection in a patient with a history of chronic obstructive pulmonary disease where the organism was identified by using molecular methods. m. chimaera was identified from fresh lung tissue and from an instrument-negative mycobacterial growth indicator tube broth culture. the utility of using sequence analysis of the internal transcribed spacer region for the rapid identification of a slow-growing nontuberculous mycobacterium spp. where con ... | 2009 | 19216940 |
| proposal to elevate mycobacterium avium complex its sequevar mac-q to mycobacterium vulneris sp. nov. | the mycobacterium avium complex (mac) consists of four recognized species, mycobacterium avium, mycobacterium colombiense, mycobacterium intracellulare and mycobacterium chimaera, and a variety of other strains that may be members of undescribed taxa. we report on two isolates of a scotochromogenic, slowly growing, non-tuberculous mycobacterium species within the m. avium complex from a lymph node and an infected wound after a dogbite of separate patients in the netherlands. the extrapulmonary i ... | 2009 | 19620376 |
| genotyping of mycobacterium avium complex organisms using multispacer sequence typing. | mycobacterium avium complex (mac) currently comprises eight species of environmental and animal-associated, slowly-growing mycobacteria: mycobacterium avium, mycobacterium intracellulare, mycobacterium chimaera, mycobacterium colombiense, mycobacterium arosiense , mycobacterium bouchedurhonense, mycobacterium marseillense and mycobacterium timonense. in humans, mac organisms are responsible for opportunistic infections whose unique epidemiology remains poorly understood, in part due to the lack ... | 2010 | 19926652 |
| surviving within the amoebal exocyst: the mycobacterium avium complex paradigm. | most of environmental mycobacteria have been previously demonstrated to resist free-living amoeba with subsequent increased virulence and resistance to antibiotics and biocides. the mycobacterium avium complex (mac) comprises of environmental organisms that inhabit a wide variety of ecological niches and exhibit a significant degree of genetic variability. we herein studied the intra-ameobal location of all members of the mac as model organisms for environmental mycobacteria. | 2010 | 20359345 |
| prosthetic valve endocarditis and bloodstream infection due to mycobacterium chimaera. | prosthetic valve endocarditis (pve) due to fast-growing nontuberculous mycobacteria (ntm) has been reported anecdotally. reports of pve with slowly growing ntm, however, are lacking. we present here one case of pve and one case of bloodstream infection caused by mycobacterium chimaera. randomly amplified polymorphic dna (rapd)-pcr indicated a relatedness of the two m. chimaera strains. both patients had heart surgery 2 years apart from each other. a nosocomial link was not detected. | 2013 | 23536407 |
| evaluation of the new genotype ntm-dr kit for the molecular detection of antimicrobial resistance in non-tuberculous mycobacteria. | non-tuberculous mycobacteria (ntm) are emerging pathogens causing difficult-to-treat infections. we tested a new assay (genotype ntm-dr) that detects natural and acquired resistance mechanisms to macrolides and aminoglycosides in frequently isolated ntm species. | 2017 | 28333340 |
| clinical characteristics and treatment outcomes of pulmonary disease caused by mycobacterium chimaera. | mycobacterium chimaera is a recently described species distinct from m. intracellulare. m. chimaera is regarded as less virulent than m. intracellulare. using multi-locus sequence-based identification, m. chimaera lung disease was diagnosed in 11 patients. clinical characteristics and outcomes of m. chimaera lung disease were comparable to m. intracellulare lung disease. | 2016 | 27720208 |
| inverse correlation between salt tolerance and host-adaptation in mycobacteria. | the genus mycobacterium includes host-adapted organisms regarded as obligate and opportunistic pathogens and environmental organisms. factors contributing to this wide range of adaptations are poorly known. | 2016 | 27129386 |
| bacteriological and virulence study of a mycobacterium chimaera isolate from a patient in china. | a clinical isolate from a patient was identified as mycobacterium chimaera, a recently identified species of nontuberculous mycobacteria. the biochemical and molecular identity, drug sensitivity and virulence of this isolated strain were investigated. 16s rrna, the 16s-23s its, hsp65 and rpob were amplified, and their sequence similarities with other mycobacteria were analyzed. the minimum inhibitory concentrations of 22 anti-microbial agents against this isolate were established, and the virule ... | 2015 | 25763937 |
| synergistic activity of rifampicin and ethambutol against slow-growing nontuberculous mycobacteria is currently of questionable clinical significance. | a key issue in the treatment of disease caused by slow-growing nontuberculous mycobacteria is the limited association between in vitro minimum inhibitory concentrations (mics) of rifampicin and ethambutol alone and the in vivo outcome of treatment with these drugs. combined susceptibility testing to rifampicin and ethambutol could provide a more realistic view of the efficacy of these drugs. in this study, mycobacterium avium (n = 5), mycobacterium chimaera (n = 6), mycobacterium intracellulare ... | 2013 | 23664674 |
| insidious risk of severe mycobacterium chimaera infection in cardiac surgery patients. | an urgent uk investigation was launched to assess risk of invasive mycobacterium chimaera infection in cardiothoracic surgery and a possible association with cardiopulmonary bypass heater-cooler units following alerts in switzerland and the netherlands. | 2017 | 27927870 |
| healthcare-associated mycobacterium chimaera transmission and infection prevention challenges: role of heater-cooler units as a water source in cardiac surgery. | | 2017 | 27927869 |
| authors' correction for euro surveill. 2016;21(46). | | 2016 | 27922449 |
| mycobacterium chimaera colonisation of heater-cooler units (hcu) in western australia, 2015: investigation of possible iatrogenic infection using whole genome sequencing. | following the reported link between heater-cooler unit (hcu) colonisation with mycobacterium chimaera and endocarditis, mycobacterial sampling of all hcus in use in western australia was initiated from august 2015, revealing m. chimaera colonisation in 10 of 15 hcus. after m. chimaera was isolated from a pleural biopsy from a cardiothoracic patient who may have been exposed to a colonised hcu, a whole genome sequencing investigation was performed involving 65 specimens from 15 hcus across five h ... | 2016 | 27918263 |
| mycobacterium chimaera infections associated with heater-cooler units (hcu): closing another loophole in patient safety. | | 2016 | 27918256 |
| clinical implications of mycobacterium chimaera detection in thermoregulatory devices used for extracorporeal membrane oxygenation (ecmo), germany, 2015 to 2016. | mycobacterium chimaera, a non-tuberculous mycobacterium, was recently identified as causative agent of deep-seated infections in patients who had previously undergone open-chest cardiac surgery. outbreak investigations suggested an aerosol-borne pathogen transmission originating from water contained in heater-cooler units (hcus) used during cardiac surgery. similar thermoregulatory devices are used for extracorporeal membrane oxygenation (ecmo) and m. chimaera might also be detectable in ecmo tr ... | 2016 | 27918254 |
| environmental nontuberculous mycobacteria in the hawaiian islands. | lung disease caused by nontuberculous mycobacteria (ntm) is an emerging infectious disease of global significance. epidemiologic studies have shown the hawaiian islands have the highest prevalence of ntm lung infections in the united states. however, potential environmental reservoirs and species diversity have not been characterized. in this cross-sectional study, we describe molecular and phylogenetic comparisons of ntm isolated from 172 household plumbing biofilms and soil samples from 62 non ... | 2016 | 27780201 |
| draft genome sequence of mycobacterium chimaera type strain fl-0169. | we report here the draft genome sequence of the type strain mycobacterium chimaera fl-0169, a member of the mycobacterium avium complex (mac). m. chimaera fl-0169(t) was isolated from a patient in italy and is highly similar to strains of m. chimaera isolated in ireland, although fl-0169(t) possesses unique virulence genes. | 2017 | 28232435 |
| evaluation of the genotype ntm-dr assay for identification of mycobacterium chimaera. | identification of species within the mycobacterium avium complex (mac) is difficult and most current diagnostic laboratory tests cannot distinguish between species included in the complex. differentiation of species within the mac is important as mycobacterium chimaera has recently emerged as major cause of invasive cardiovascular infections following open heart surgery. a new commercial diagnostic assay, genotype ntm-dr ver. 1.0, is intended to differentiate between three species within the mac ... | 2017 | 28356415 |
| in vitro activity of amikacin against isolates of mycobacterium avium complex with proposed mic breakpoints and finding of a 16s rrna gene mutation in treated isolates. | amikacin is a major drug used for the treatment of mycobacterium avium complex (mac) disease, but standard laboratory guidelines for susceptibility testing are not available. this study presents in vitro amikacin mics for 462 consecutive clinical isolates of the mac using a broth microdilution assay. approximately 50% of isolates had amikacin mics of 8 μg/ml, and 86% had mics of ≤16 μg/ml. of the eight isolates (1.7%) with mics of 64 μg/ml, five had an mic of 32 μg/ml on repeat testing. ten isol ... | 2013 | 23946523 |
| persistent immune thrombocytopenia heralds the diagnosis of mycobacterium chimaera prosthetic valve endocarditis. | a 63 year old female was admitted for investigation of worsening renal insufficiency. during hospitalization she developed persistent immune thrombocytopenia refractory to supportive or immunosuppressive treatment. she was diagnosed with mycobacterium chimaera prosthetic valve endocarditis and thrombocytopenia resolved with anti-mycobacterial therapy. | 2017 | 27909685 |
| mycobacterium chimaera pulmonary infection complicating cystic fibrosis: a case report. | abstract: | 2011 | 21939536 |
| mycobacterium chimaera causes tuberculosis-like infection in a male patient with anorexia nervosa. | here we present a 27-year-old male patient-with a known prolonged history of anorexia nervosa (an) -suffering from tuberculosis like infection. at the time he was admitted to clinical treatment, he had developed fever up to 40°c and survived on a body mass index of 11.8. in this case, mycobacterium chimaera, generally recognized for low pathogenicity, was identified as the causative agent. remission from lung infection was achieved after antibiotic treatment according to laboratory susceptibilit ... | 2011 | 21656541 |
| mycobacterium marseillense sp. nov., mycobacterium timonense sp. nov. and mycobacterium bouchedurhonense sp. nov., members of the mycobacterium avium complex. | an rpob sequence-based evaluation of 100 mycobacterium avium complex (mac) clinical isolates led to the identification of five respiratory tract isolates that were potential representatives of three novel mac species. distinctive phenotypic features of isolates 62863 and 5356591(t) included a pseudomycelium morphology and both esterase and acid phosphatase activities. these two isolates exhibited sequence similarities of 99.8 % for the 16s rrna gene, 86.3 and 86.1 % for 16s-23s rrna gene interna ... | 2009 | 19628609 |
| mycobacterium mantenii sp. nov., a pathogenic, slowly growing, scotochromogenic species. | slowly growing, scotochromogenic bacteria of a novel mycobacterium species were isolated from lymph node samples in two children and pulmonary samples in two elderly patients from different regions in the netherlands as well as from a surface water sample in zambia. its 16s rrna gene, 16s-23s internal transcribed spacer (its), hsp65 and rpob gene sequences are unique in comparison with other mycobacteria. phylogenetic analysis based on the 16s rrna gene sequence revealed that these micro-organis ... | 2009 | 19625425 |
| rpob sequence-based identification of mycobacterium avium complex species. | the mycobacterium avium complex (mac) comprises slowly growing mycobacteria responsible for opportunistic infections and zoonoses. the ability to speciate mac isolates in the clinical microbiology laboratory is critical for determining the organism implicated in clinical disease and for epidemiological investigation of the source of infection. investigation of a 711 bp variable fragment of rpob flanked by the myco-f/myco-r primers found a 0.7-5.1 % divergence among mac reference strains, with my ... | 2008 | 19047739 |
| proposal to elevate the genetic variant mac-a, included in the mycobacterium avium complex, to species rank as mycobacterium chimaera sp. nov. | the possibility that the strains included within the mycobacterium avium complex (mac), but not belonging either to m. avium or to mycobacterium intracellulare, may be members of undescribed taxa, has already been questioned by several taxonomists. a very homogeneous cluster of 12 strains characterized by identical nucleotide sequences both in the 16s rdna and in the 16s-23s internal transcribed spacer was investigated. similar strains, previously reported in the literature, had been assigned ei ... | 2004 | 15280303 |
| mycobacterium chimaera spread from heating and cooling units in heart surgery. | | 2017 | 28177865 |
| mycobacterium chimaera and cardiac surgery. | there is an ongoing investigation into infections with non-tuberculous mycobacteria associated with contaminated heater-cooler units used in cardiac surgery. the overall risk is low, but surgical site and disseminated infections have been reported, including one possible case in australia, mainly with surgery involving implantation of prosthetic material. mycobacterium chimaera infection should be considered in patients who have previously undergone surgery with cardiopulmonary bypass and who pr ... | 2017 | 28208046 |
| mycobacterium chimaera: the ethical duty to disclose the minimal risk of infection to exposed patients. | | 2017 | 28351477 |
| [one year of infectious diseases : a review]. | several outbreaks have made the news in 2016 : ebola has come at an end, zika is booming and a resurgence of yellow fever takes place in africa. in switzerland, two hospital outbreaks have been reported, caused by mycobacterium chimerae and burkholderia cepacia. a major new article has consolidated the notion that prolonged antibiotic therapy is unnecessary in lyme disease. as multiresistant bacteria are increasing in frequency, innovative therapeutic approaches are under development. for lung i ... | 2017 | 28703540 |
| a target-specific assay for rapid and quantitative detection of mycobacterium chimaera dna. | mycobacterium chimaera is an opportunistic environmental mycobacterium, belonging to the mycobacterium intracellulare complex. although most commonly associated with pulmonary disease, there has been growing awareness of invasive m. chimaera infections following cardiac surgery. investigations suggest world-wide spread of a specific m. chimaera clone, associated with contaminated hospital heater-cooler units used during the surgery. given the global dissemination of this clone, its potential to ... | 2017 | 28381604 |
| accurate differentiation of mycobacterium chimaera from mycobacterium intracellulare by maldi-tof ms analysis. | the increasing number of infections caused by nontuberculous mycobacteria (ntm) has prompted the need for rapid and precise identification methods of these pathogens. several studies report the applicability of maldi-tof mass spectrometry (ms) for identification of ntm. however, some closely related species have very similar spectral mass fingerprints, and until recently, mycobacterium chimaera and m. intracellulare could not be separated from each other by maldi-tof ms. | 2017 | 28504926 |
| characterization of a novel variant of mycobacterium chimaera. | in this study, nonchromogenic mycobacteria were isolated from pulmonary samples of three patients in the netherlands. all isolates had identical, unique 16s rrna gene and 16s-23s its sequences, which were closely related to those of mycobacterium chimaera and mycobacterium marseillense. the biochemical features of the isolates differed slightly from those of m. chimaera, suggesting that the isolates may represent a possible separate species within the mycobacterium avium complex (mac). however, ... | 2012 | 22700551 |
| absence of mycobacterium intracellulare and presence of mycobacterium chimaera in household water and biofilm samples of patients in the united states with mycobacterium avium complex respiratory disease. | recent studies have shown that respiratory isolates from pulmonary disease patients and household water/biofilm isolates of mycobacterium avium could be matched by dna fingerprinting. to determine if this is true for mycobacterium intracellulare, household water sources for 36 patients with mycobacterium avium complex (mac) lung disease were evaluated. mac household water isolates from three published studies that included 37 additional mac respiratory disease patients were also evaluated. speci ... | 2013 | 23536397 |
| evaluation of vitek ms for rapid classification of clinical isolates belonging to mycobacterium avium complex. | we evaluated the ability of the vitek ms system to classify clinical pulmonary mycobacterium avium complex isolates compared to multilocus sequence analysis. vitek ms accurately identified 55% of the isolates as m. avium and 18% as m. intracellulare, but misidentified 24 (27%) mycobacterium chimaera isolates as mycobacterium intracellulare. | 2015 | 25445119 |
| notes from the field: mycobacterium chimaera contamination of heater-cooler devices used in cardiac surgery - united states. | in the spring of 2015, investigators in switzerland reported a cluster of six patients with invasive infection with mycobacterium chimaera, a species of nontuberculous mycobacterium ubiquitous in soil and water. the infected patients had undergone open-heart surgery that used contaminated heater-cooler devices during extracorporeal circulation (1). in july 2015, a pennsylvania hospital also identified a cluster of invasive nontuberculous mycobacterial infections among open-heart surgery patients ... | 2016 | 27740609 |
| mycobacterium chimaera in heater-cooler units in denmark related to isolates from the united states and united kingdom. | mycobacterium chimaera was present at high rates (>80%) in heater-cooler units (hcus) from all 5 thoracic surgery departments in denmark. isolates were clonal to hcu-associated isolates from the united states (including some from patients) and united kingdom. however, m. chimaera from 2 brands of hcu were genetically distinct. | 2017 | 28035898 |
| clinical and histopathologic ocular findings in disseminated mycobacterium chimaera infection after cardiothoracic surgery. | to investigate and characterize clinical and histopathologic ocular findings in patients with disseminated infection with mycobacterium chimaera, a slow-growing nontuberculous mycobacterium (ntm), subsequent to cardiothoracic surgery. | 2017 | 27871762 |
| complete genome sequence of mycobacterium chimaera strain ah16. | mycobacterium chimaera is a nontuberculous mycobacterial species that causes cardiovascular, pulmonary, and postsurgical infections. here, we report the first complete genome sequence of m. chimaera this genome is 6.33 mbp, with a g+c content of 67.56%, and encodes 4,926 protein-coding genes, as well as 74 trnas, one ncrna, and three rrna genes. | 2016 | 27881537 |
| frequency of mycobacterium chimaera among belgian patients, 2015. | mycobacterium chimaera arouses an increasing public health concern, as this non-tuberculous mycobacterium (ntm) has recently been associated with life-threatening cardiac infections. m. chimaera and mycobacteriumintracellulare are genetically very close but recently appeared to present different epidemiological and clinical significance. therefore, it has become important for laboratories to use adequate techniques allowing precise species identification. to date, most commercially available lab ... | 2016 | 27902393 |
| long-range dhps mutations unexpectedly increase mycobacterium chimaera susceptibility to sulfonamides. | the two closely related mycobacteria, mycobacterium intracellulare and mycobacterium chimaera, exhibit a more than two-fold difference in their in vitro susceptibility to sulfonamides. sulfonamides are antibiotics targeting the 6-hydroxymethyl-7,8-dihydropteroate synthase (dhps) enzyme involved in the folate synthesis pathway. comparing the dhps gene sequence in six m. intracellulare and m. chimaera types trains and clinical isolates yielded only four amino acid changes. in silico structural mod ... | 2013 | 27873610 |
| mycobacterium paraintracellulare sp. nov., for the genotype int-1 of mycobacterium intracellulare. | three mycobacterial strains, isolated from independent korean patients with pulmonary infections, belonging to the mycobacterium intracellulare genotype 1 (int-1) were characterized using a polyphasic approach. the sequences of the 16s rrna gene and internal transcribed spacer 1 (its1) of the int-1 strains were identical to those of mycobacterium intracellulare atcc 13950t. however, multilocus sequence typing (mlst) analysis targeting five housekeeping genes (hsp65, rpob, argg, gnd and pgm) reve ... | 2016 | 27189351 |
| mycobacterium chimaera outbreak associated with heater-cooler devices: piecing the puzzle together. | an outbreak of invasive mycobacterium chimaera infections associated with heater-cooler devices (hcds) has now affected patients in several countries on different continents. clinical infections are characterized by delayed diagnosis, inadequate treatment response to antimicrobial agents, and poor prognosis. outbreak investigators found m. chimaera in hcd water circuits and air samples while hcds were running, suggesting that transmission from the hcd to the surgical site occurs via the airborne ... | 2017 | 27839530 |
| dose response models and a quantitative microbial risk assessment framework for the mycobacterium avium complex that account for recent developments in molecular biology, taxonomy, and epidemiology. | mycobacterium avium complex (mac) is a group of environmentally-transmitted pathogens of great public health importance. this group is known to be harbored, amplified, and selected for more human-virulent characteristics by amoeba species in aquatic biofilms. however, a quantitative microbial risk assessment (qmra) has not been performed due to the lack of dose response models resulting from significant heterogeneity within even a single species or subspecies of mac, as well as the range of huma ... | 2017 | 27915187 |
| disseminated mycobacterium chimaera infection after cardiothoracic surgery. | ten case reports of disseminated mycobacterium chimaera infections associated with cardiovascular surgery were published from europe. we report 3 cases of disseminated m chimaera infections with histories of aortic graft and/or valvular surgery within the united states. two of 3 patients demonstrated ocular involvement, a potentially important clinical finding. | 2016 | 27703994 |
| reemergence of mycobacterium chimaera in heater-cooler units despite intensified cleaning and disinfection protocol. | invasive mycobacterium chimaera infections after open-heart surgery have been reported internationally. these devastating infections result from aerosols generated by contaminated heater-cooler units used with extracorporeal circulation during surgery. despite intensified cleaning and disinfection, surveillance samples from factory-new units acquired during 2014 grew nontuberculous mycobacteria after a median of 174 days. | 2016 | 27649345 |
| contamination during production of heater-cooler units by mycobacterium chimaera potential cause for invasive cardiovascular infections: results of an outbreak investigation in germany, april 2015 to february 2016. | invasive infections with mycobacterium chimaera were reported in patients with previous open chest surgery and exposure to contaminated heater-cooler units (hcus). we present results of the surveillance of clinical cases and of contaminated hcus as well as environmental investigations in germany up until february 2016. clinical infections occurred in five male german cases over 50 years of age (range 53-80). cases had been exposed to hcus from one single manufacturer during open chest surgery up ... | 2016 | 27168588 |
| decontamination of heater-cooler units associated with contamination by atypical mycobacteria. | non-tuberculosis mycobacteria such as mycobacterium chimaera are found widely in hospital water systems. invasive m. chimaera infections have recently been attributed to heater-cooler units (hcus) of cardiopulmonary bypass equipment. | 2016 | 27112044 |
| heater-cooler units: contamination of crucial devices in cardiothoracic surgery. | several cases of mycobacterium chimaera infection have recently been reported in cardiosurgical patients. so-called heater-cooler units (hcus) used in cardiosurgical procedures are suspected to be the reservoir for pathogen growth and dissemination. | 2016 | 27101883 |
| transmission of mycobacterium chimaera from heater-cooler units during cardiac surgery despite an ultraclean air ventilation system. | heater-cooler units (hcus) were recently identified as a source of mycobacterium chimaera causing surgical site infections. we investigated transmission of this bacterium from hcus to the surgical field by using a thermic anemometer and particle counter, videotape of an operating room equipped with an ultraclean laminar airflow ventilation system, and bacterial culture sedimentation plates in a nonventilated room. smoke from the hcu reached the surgical field in 23 s by merging with ultraclean a ... | 2016 | 27070958 |
| healthcare outbreaks associated with a water reservoir and infection prevention strategies. | hospital water may serve as a reservoir of healthcare-associated pathogens, and contaminated water can lead to outbreaks and severe infections. the clinical features of waterborne outbreaks and infections as well as prevention strategies and control measures are reviewed. the common waterborne pathogens were bacteria, including legionella and other gram-negative bacteria, and nontuberculous mycobacteria, although fungi and viruses were occasionally described. these pathogens caused a variety of ... | 2016 | 26936670 |
| draft genome sequences of three mycobacterium chimaera respiratory isolates. | mycobacterium chimaera is an opportunistic human pathogen implicated in both pulmonary and cardiovascular infections. here, we report the draft genome sequences of three strains isolated from human respiratory specimens. | 2015 | 26634757 |
| healthcare-associated prosthetic heart valve, aortic vascular graft, and disseminated mycobacterium chimaera infections subsequent to open heart surgery. | we identified 10 patients with disseminated mycobacterium chimaera infections subsequent to open-heart surgery at three european hospitals. infections originated from the heater-cooler unit of the heart-lung machine. here we describe clinical aspects and treatment course of this novel clinical entity. | 2015 | 26188001 |
| prolonged outbreak of mycobacterium chimaera infection after open-chest heart surgery. | invasive mycobacterium chimaera infections were diagnosed in 2012 in 2 heart surgery patients on extracorporeal circulation. we launched an outbreak investigation to identify the source and extent of the potential outbreak and to implement preventive measures. | 2015 | 25761866 |
| disseminated mycobacterium chimaera presenting as vertebral osteomyelitis. | mycobacterium chimaera, a member of the mycobacterium avium complex, is a slow-growing, nontuberculous mycobacterium associated with outbreaks in cardiac-surgery patients supported on heart-lung machines. we report a case of an elderly woman on chronic prednisone who presented with a six-month history of worsening chronic back pain, recurrent low-grade fevers, and weight loss. imaging identified multilevel vertebral osteomyelitis and lumbar soft-tissue abscess. abscess culture identified m. chim ... | 2017 | 28523197 |
| mycobacterium yongonense sp. nov., a slow-growing non-chromogenic species closely related to mycobacterium intracellulare. | a slow-growing non-chromogenic mycobacterium was isolated from a patient with pulmonary disease. phenotypically, strain 05-1390(t) was similar to mycobacterium intracellulare atcc 13950(t). the 16s rrna gene sequence (1385 bp) of strain 05-1390(t) showed a high degree of similarity to those of the m. intracellulare complex, namely mycobacterium marseillense 5351974(t) (100 %), m. intracellulare atcc 13950(t) (99.8 %) and mycobacterium chimaera dsm 44623(t) (99.9 %). phylogenetic analysis based o ... | 2013 | 22427442 |
| healthcare-associated mycobacterium chimaera infection subsequent to heater-cooler device exposure during cardiac surgery. | a series of reports in the united states and europe have linked mycobacterium chimaera infections to contaminated heater-cooler devices used during cardiac surgery. heater-cooler devices commonly are used for cardiopulmonary bypass during cardiac surgery. m. chimaera is a slow-growing nontuberculous mycobacterium that has been shown to cause cardiac complications that can lead to fatal disease following cardiac surgery. given that more than 250,000 cardiothoracic surgical procedures requiring ca ... | 2017 | 28838729 |
| elimination of mycobacterium chimaera in a heater cooler device used during on-pump cardiothoracic surgery. | since 2014, several infections with non-tuberculous mycobacteria (ntm) belonging to the species mycobacterium ( m.) chimaera have been associated with the use of heater-cooler devices during on-pump cardiothoracic surgery both in european countries and the united states of america. infections have been detected after surgery, with a delay of a few months and up to five years. bacterial contamination of heater-cooler devices has also been described without any associated infections. in many centr ... | 2017 | 28820031 |
| decontamination of an extracorporeal membrane oxygenator contaminated with mycobacterium chimaera. | water samples taken from extracorporeal membrane oxygenator (ecmo) devices used at university hospitals birmingham yielded high total viable counts (tvcs) containing a variety of microorganisms, including m. chimaera. disinfection resulted in the reduction of tvcs and eradication of mycobacterium chimaera. weekly disinfection and water sampling are required to manage the water quality in these devices. infect control hosp epidemiol 2017;1-3. | 2017 | 28803570 |
| comparison of methods available for the identification of mycobacterium chimaera. | mycobacterium chimaera is a recently described nontuberculous mycobacterium belonging to the m. avium complex (mac). because this species is implicated in a worldwide outbreak due to contaminated heater-cooler unit water tanks during open-heart surgery, it becomes mandatory for clinical microbiology laboratories to be able to differentiate m. chimaera from the other mac species, especially m. intracellulare. such identification was so far restricted to specialized laboratories, since it required ... | 2017 | 28782649 |
| complete genome sequence of mycobacteriumchimaera strain cdc2015-22-71. | mycobacterium chimaera is a nontuberculous mycobacterium species commonly found in the environment. here, we report the first complete genome sequence of a strain from the investigation of invasive infections following open-heart surgeries that used contaminated livanova sorin stockert 3t heater-cooler devices. | 2017 | 28774973 |
| mycobacterium chimaera: unraveling a mystery through genomics. | | 2017 | 28711587 |
| global outbreak of severe mycobacterium chimaera disease after cardiac surgery: a molecular epidemiological study. | since 2013, over 100 cases of mycobacterium chimaera prosthetic valve endocarditis and disseminated disease were notified in europe and the usa, linked to contaminated heater-cooler units (hcus) used during cardiac surgery. we did a molecular epidemiological investigation to establish the source of these patients' disease. | 2017 | 28711585 |
| epidemiologic review of veterans health administration patients with isolation of nontuberculous mycobacteria after cardiopulmonary bypass procedures. | we evaluated the isolation of postoperative nontuberculous mycobacteria (ntm) associated with heater-cooler devices (hcds) used during cardiopulmonary bypass (cpb) surgery in the veterans health administration from january 1, 2010, to december 31, 2016. in more than 38,000 cpb procedures, ntm was isolated in 111 patients; 1 mycobacterium chimaera mediastinitis case and 1 respiratory isolate were found. infect control hosp epidemiol 2017;38:1103-1106. | 2017 | 28693623 |
| mycobacterium chimaera infection after cardiac surgery: first canadian outbreak. | recently reported in europe and united states, disseminated mycobacterium chimaera infection is a novel clinical entity linked to point contamination of stockert 3t heater-cooler units used for cardiopulmonary bypass. we present here the first two cases in canada. both patients presented with nonspecific extracardiac symptoms 1 year after undergoing minimally invasive mitral surgical repair. before the right diagnosis was established, the patients were initially treated with prednisone for suspe ... | 2017 | 28633259 |
| mycobacterium chimaera isolates from heater-cooler units, united kingdom. | | 2017 | 28628453 |
| mycobacterium chimaera: an under-diagnosed pathogen in developing countries? | | 2017 | 28606857 |
| global mycobacterium chimaera outbreak: challenges for the near future. | | 2017 | 28545830 |
| mycobacterium chimaera infections associated with heater-cooler units in cardiac surgery. | mycobacterium chimaera infections following cardiac surgery have been reported from an increasing number of countries. these infections are characterized by a poor prognosis with a case fatality rate around 50% despite treatment. since the first description in 2013, our understanding has grown steadily. several outbreak investigations, case series, and experiments with heater-cooler units (hcus) have been published. this review summarizes the current knowledge. | 2017 | 28538044 |
| microbiological problems and biofilms associated with mycobacterium chimaera in heater-cooler units used for cardiopulmonary bypass. | the role of heater-cooler units (hcus) in the transmission of mycobacterium chimaera during open heart surgery has been recognized since 2013. subsequent investigations uncovered a remarkable global outbreak reflecting the wide distribution of implicated devices. hcus are an essential component of cardiopulmonary bypass operations and their withdrawal would severely affect capacity for life-saving cardiac surgery. however, studies have demonstrated that many hcus are contaminated with a wide ran ... | 2017 | 28532976 |
| mycobacterium chimaera - a new threat for cardiac surgical patients? | an outbreak of invasive mycobacterium chimaera infections associated with "heater-cooler" devices in patients treated with cardiac surgery has been described worldwide. the authors summarize the current state of knowledge regarding the epidemiology, diagnostics, treatment, and prevention of mycobacterium chimaera infections in patients after cardiothoracic surgery. | 2017 | 28515744 |
| mycobacterium chimaera left ventricular assist device infections. | a global outbreak of invasive mycobacterium chimaera infections after cardiac surgery has recently been linked to bioaerosols from contaminated heater-cooler units. the majority of cases have occurred after valvular surgery or aortic graft surgery and nearly half have resulted in death. to date, infections in patients with left ventricular assist devices (lvads) have not been characterized in the literature. we report two cases of device-associated m. chimaera infection in patients with continuo ... | 2017 | 28508409 |
| a year in the life of a contaminated heater-cooler unit with mycobacterium chimaera? | objective heater-cooler units (hcus) have been shown to be a source of mycobacterium chimaera infections. for the past year, weekly water samples have been taken from hcus used at university hospitals birmingham (uhb) nhs foundation trust. we report the microbial contamination of the hcus over a year detailing the decontamination regimes applied at uhb to reduce the microbial load. design observational study setting uhb is a tertiary referral teaching hospital in birmingham, united kingdom, that ... | 2017 | 28473009 |
| mycobacterium chimaera infections associated with contaminated heater-cooler devices for cardiac surgery: outbreak management. | this paper focuses on assisting hospitals in establishing a rapid response for identification, notification, and evaluation of exposed patients, and management of hcds with regards to placement and containment, environmental culturing, and disinfection.the global outbreak of mycobacteriurm chimaera infections associated with heater-cooler devices (hcds) presents several important, unique challenges for the infection prevention community. the primary focus of this paper is to assist hospitals in ... | 2017 | 28430899 |
| heater-cooler unit contamination with mycobacterium chimaera - potential harmful risks for cardiac surgery patients. | | 2017 | 28421583 |