| cotton leaf curl gezira virus-satellite dnas represent a divergent, geographically isolated nile basin lineage: predictive identification of a satdna rep-binding motif. | cotton leaf curl gezira virus (clcugv), a species of the genus begomovirus (family geminiviridae), was recently cloned from cotton, okra, and sida alba plants exhibiting leaf-curling and vein-thickening symptoms in sudan. here, we describe a previously unknown lineage of single-stranded dna satellite (satdna) molecules, which are associated with clcugv, and are required for development of characteristic disease symptoms. co-inoculation of cotton and nicotiana benthamiana plants with satdnas clon ... | 2004 | 15826909 |
| complete nucleotide sequences of okra isolates of cotton leaf curl gezira virus and their associated dna-beta from niger. | okra (abelmoschus esculentus) is a major crop in niger. in the fall of 2007, okra leaf curl disease was observed in niger and the begomovirus and dna-beta satellite were found associated with the disease. the complete nucleotide sequences of dna-a (fj469626 and fj469627) and associated dna-beta satellites (fj469628 and fj469629) were determined from two samples. this is the first report of molecular characterization of okra-infecting begomovirus and their associated dna-beta from niger. the bego ... | 2009 | 19156351 |
| roles and interactions of begomoviruses and satellite dnas associated with okra leaf curl disease in mali, west africa. | okra leaf curl disease (olcd) is a major constraint on okra (abelmoschus esculentus) production in west africa. two monopartite begomoviruses (okra virus-1 and okra virus-2), a betasatellite and a dna1 satellite are associated with olcd in mali. okra virus-1 is an isolate of okra yellow crinkle virus (oycrv), okra virus-2 is a recombinant isolate of cotton leaf curl gezira virus (clcugv) and the betasatellite is a variant of cotton leaf curl gezira betasatellite (clcugb). cloned dna of oycrv and ... | 2009 | 19264648 |
| molecular diversity of cotton leaf curl gezira virus isolates and their satellite dnas associated with okra leaf curl disease in burkina faso. | okra leaf curl disease (olcd) is a major constraint on okra (abelmoschus esculentus) production and is widespread in africa. using a large number of samples representative of the major growing regions in burkina faso (bf), we show that the disease is associated with a monopartite begomovirus and satellite dna complexes. twenty-three complete genomic sequences of cotton leaf curl gezira virus (clcugv) isolates associated with olcd, sharing 95 to 99% nucleotide identity, were cloned and sequenced. ... | 2010 | 20178575 |
| the merging of two dynasties-identification of an african cotton leaf curl disease-associated begomovirus with cotton in pakistan. | cotton leaf curl disease (clcud) is a severe disease of cotton that occurs in africa and pakistan/northwestern india. the disease is caused by begomoviruses in association with specific betasatellites that differ between africa and asia. during survey of symptomatic cotton in sindh (southern pakistan) cotton leaf curl gezira virus (clcugv), the begomovirus associated with clcud in africa, was identified. however, the cognate african betasatellite (cotton leaf curl gezira betasatellite) was not f ... | 2011 | 21637815 |
| two genetically related begomoviruses causing tomato leaf curl disease in togo and nigeria differ in virulence and host range but do not require a betasatellite for induction of disease symptoms. | tomato leaf curl disease (tolcd) has emerged as a major constraint on tomato production in some parts of west africa. in this study, begomoviruses associated with tolcd in togo and nigeria were characterized, as well as a betasatellite associated with the disease in togo. the genome organization of both viruses is typical of old world monopartite begomoviruses. sequence analysis revealed that the begomovirus from togo is a variant of tomato leaf curl kumasi virus (tolckuv) from ghana, and it is ... | 2011 | 22057578 |
| evolution of african cassava mosaic virus by recombination between bipartite and monopartite begomoviruses. | cassava mosaic disease (cmd) is a major constraint on cassava cultivation in africa. the disease is endemic and is caused by seven distinct cassava mosaic geminiviruses (cmgs), some of them including several variants. | 2012 | 22416906 |
| identification of a disease complex involving a novel monopartite begomovirus with beta- and alphasatellites associated with okra leaf curl disease in oman. | okra leaf curl disease (olcd) is an important viral disease of okra in tropical and subtropical areas. the disease is caused by begomovirus-satellite complexes. a begomovirus and associated betasatellite and alphasatellite were identified in symptomatic okra plants from barka, in the al-batinah region of oman. analysis of the begomovirus sequences showed them to represent a new begomovirus most closely related to cotton leaf curl gezira virus (clcugev), a begomovirus of african origin. the seque ... | 2014 | 24287711 |
| molecular characterization of begomoviruses and dna satellites associated with okra leaf curl disease in cameroon. | okra leaf curl disease (olcd) is the most important viral disease of okra in west africa. in this study, a complex of begomoviruses and associated dna satellites were identified in symptomatic okra plants from southwestern cameroon. sequence analyses showed that two of the plants (lik1 and njo5) were infected with a begomovirus being a recombinant of cotton leaf curl gezira virus (clcugev) and okra yellow crinkle virus (oycrv). the recombinant genome shared highest nucleotide identity with isola ... | 2013 | 23535770 |
| the prediction of a new clcud epidemic in the old world. | cotton leaf curl disease (clcud), the most complex disease of cotton, is a major limiting biotic factor to worldwide cotton productivity. several whitefly-transmitted monopartite begomoviruses causing clcud have been characterized and designated as clcud-associated begomoviruses. despite of being reported over 100 years ago in africa, clcud became economically pandemic causing massive losses to cotton production in pakistan and india during past couple of decades. in asia, cotton has faced two m ... | 2017 | 28469604 |
| molecular diagnostic development for begomovirus-betasatellite complexes undergoing diversification: a case study. | at least five begomoviral species that cause leaf curl disease of cotton have emerged recently in asia and africa, reducing fiber quality and yield. the potential for the spread of these viruses to other cotton-vegetable growing regions throughout the world is extensive, owing to routine, global transport of alternative hosts of the leaf curl viruses, especially ornamentals. the research reported here describes the design and validation of polymerase chain reaction (pcr) primers undertaken to fa ... | 2017 | 28438632 |