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multiplication of vesicular stomatitis virus in the leafhopper peregrinus maidis (ashm.), a vector of a plant rhabdovirus.vesicular stomatitis virus (vsv) was found to multiply efficiently in whole peregrinus maidis (ashm.). the leafhopper vector of maize mosaic virus (mmv), a plant rhabdovirus. insects were inoculated with vsv by means of a microsyringe, collected at 1-day intervals and tested individually for the presence of virus. exponential virus multiplication occurred within the first 4 days, reaching titres of 10(6) p.f.u. per insect in days 5 to 10 after inoculation. these observations show that a common h ...1976182911
analysis of expressed sequence tags from maize mosaic rhabdovirus-infected gut tissues of peregrinus maidis reveals the presence of key components of insect innate immunity.the corn planthopper, peregrinus maidis, causes direct feeding damage to plants and transmits maize mosaic rhabdovirus (mmv) in a persistent-propagative manner. mmv must cross several insect tissue layers for successful transmission to occur, and the gut serves as an important barrier for rhabdovirus transmission. in order to facilitate the identification of proteins that may interact with mmv either by facilitating acquisition or responding to virus infection, we generated and analysed the gut ...201121199018
electron microscopic and physicochemical analysis of a reo-like virus of the planthopper peregrinus maidis.a virus, determined to be a member of the reoviridae, was found naturally infecting colonies of peregrinus maidis, the maize planthopper. the virus, referred to as peregrinus maidis-virus (pgmv), was found by electron microscopy throughout all p. maidis tissues examined, including salivary glands, testes, ovaries, and intestines. the virus particles were associated with fibrillar viroplasmic inclusions in the cytoplasm, of a type characteristic of the reoviridae. the particles, purified from p. ...20113182234
histopathology caused by the entomopathogenic fungi, beauveria bassiana and metarhizium anisopliae, in the adult planthopper, peregrinus maidis, a maize virus vector.the planthopper peregrinus maidis (ashmead) (hemiptera: delphacidae) is an important vector of maize viruses in tropical and subtropical areas. planthoppers are biologically controlled with several species of entomopathogenic fungi that have been isolated from these insect pests of rice in asia. beauveria bassiana (balsamo-crivelli) vuillemin and metarhizium anisopliae (metschnikoff) sorokin (hypocreales: clavicipitaceae) appear to be the most useful against planthoppers because of their ease of ...201020578956
evaluation of maize inbreds for maize stripe virus and maize mosaic virus resistance: disease progress in relation to time and the cumulative number of planthoppers.abstract five tropical maize lines were tested and compared with the susceptible control line b73 for resistance to maize stripe virus (mstv) and maize mosaic virus (mmv), both propagatively transmitted by the planthopper peregrinus maidis (homoptera: delphacidae). resistance to each virus was evaluated separately by artificial inoculations with planthoppers viruliferous for either one virus or the other. disease incidence and symptom severity progression were quantified in relation to time and ...200518943775
maize stripe virus: characteristics of a member of a new virus class.an unusual filamentous nucleoprotein about 3 nm in diameter was consistently associated with maize stripe-diseased maize. antiserum to purified nucleoprotein neutralized the infectivity of extracts from maize stripe-diseased plants suggesting that the nucleoprotein was the maize stripe virus (mstpv). the rate-zonal sedimentation pattern of the nucleoprotein on sucrose gradients was polydisperse between 51 and 70 s. cscl isopycnic centrifugation of combined nucleoprotein zones from sucrose gradie ...198118635064
a neurotropic route for maize mosaic virus (rhabdoviridae) in its planthopper vector peregrinus maidis.to investigate the dissemination route of maize mosaic virus (mmv, rhabdoviridae) in its planthopper vector peregrinus maidis (delphacidae, hemiptera), temporal and spatial distribution of mmv was studied by immunofluorescence confocal laser scanning microscopy following 1-week acquisition feeding of planthoppers on infected plants. mmv was detected 1-week post first access to diseased plants (padp) in the midgut and anterior diverticulum, 2-week padp in the esophagus, nerves, nerve ganglia and ...200817928084
pathogenicity of fungal isolates (ascomycota: hypocreales) against peregrinus maidis, delphacodes kuscheli (hemiptera: delphacidae), and dalbulus maidis (hemiptera: cicadellidae), vectors of corn diseases.preliminary screening assays were carried out on 17 isolates from five fungal species beauveria bassiana, lecanicillium muscarium, metarhizium anisopliae, isaria farinosa, and i. fumosorosea. the three most effective isolates against peregrinus maidis (hemiptera: delphacidae) were b. bassiana cep 147, cep 150, and cep 189. there were no consistent differences found in males and females regarding fungal susceptibility. however, more females than males were proportionally infected. there was not a ...200717407003
assembly and accumulation sites of maize mosaic virus in its planthopper vector.the morphology, assembly, and accumulation sites of rhabdovirus particles in peregrinus maidis planthoppers infected with a hawaiian isolate of maize mosaic virus (mmv) were studied. these particles were usually bullet-shaped, but were sometimes bacilliform, and averaged 234 and 247 nm, respectively, in length and 60 nm in width. they were found in most acini of the principal and accessory salivary glands and in brain, nerve ganglia, leg muscle, foregut, midgut, trachea, epidermis, and fat and c ...19854044200
ultrastructure of spermatozoa of peregrinus maidis (homoptera, delphacidae). 19674881298
virus particles in apparently healthy peregrinus maidis.virus-like particles were found in apparently healthy peregrinus maidis (ashm.) in venezuela. the particles were observed in the salivary gland, intestine, mycetome, adipose tissue, ovary, and hemolymph. in the cells, the particles occurred in the cytoplasm singly, in groups, free or within vesicles, and in hexagonally arranged crystals. in p. maidis from hawaii such particles were not found. however, in these insects, single particles or crystals were observed after injecting suspensions of int ...196716789083
development of rnai methods for peregrinus maidis, the corn planthopper.the corn planthopper, peregrinus maidis, is a major pest of agronomically-important crops. peregrinus maidis has a large geographical distribution and transmits maize mosaic rhabdovirus (mmv) and maize stripe tenuivirus (mspv). the objective of this study was to develop effective rnai methods for p. maidis. vacuolar-atpase (v-atpase) is an essential enzyme for hydrolysis of atp and for transport of protons out of cells thereby maintaining membrane ion balance, and it has been demonstrated to be ...201323950915
mesoionic pyrido[1,2-a]pyrimidinone insecticides: from discovery to triflumezopyrim and dicloromezotiaz.one of the greatest global challenges is to feed the ever-increasing world population. the agrochemical tools growers currently utilize are also under continuous pressure, due to a number of factors that contribute to the loss of existing products. mesoionic pyrido[1,2-a]pyrimidinones are an unusual yet very intriguing class of compounds. known for several decades, this class of compounds had not been systemically studied until we started our insecticide discovery program. this account provides ...201728825462
electron microscopic demonstration of viruslike particles in peregrinus maidis following acquisition of maize mosaic virus. 196514328611
transcriptomic response of the insect vector, peregrinus maidis, to maize mosaic rhabdovirus and identification of conserved responses to propagative viruses in hopper vectors.maize mosaic virus (mmv) is a plant-pathogenic rhabdovirus that is transmitted by the corn planthopper, peregrinus maidis, in a propagative manner. p. maidis supports long-term mmv infections with no negative effects on insect performance. to elucidate whole-body transcriptome responses to virus infection, rna-seq was used to examine differential gene expression of virus-infected adult insects, and libraries were prepared from replicated groups of virus-exposed insects and non-exposed insects. f ...201728618303
influence of a propagative plant virus on the fitness and wing dimorphism of infected and exposed insect vectors.to maximize fitness, plant pathogenic viruses may manipulate their arthropod vectors through direct and indirect (via the host plant) interactions. for many virus-vector-plant associations, insect feeding does not always lead to virus acquisition. in fact, many plant viruses, especially those that propagate into their vectors, are acquired at low rates. although the majority of insects colonizing an infected plant escape from viral infection, they are still exposed to the indirect effects (i.e. ...201323247686
influence of the corn resistance gene mv on the fitness of peregrinus maidis (hemiptera: delphacidae) and on the transmission of maize mosaic virus (rhabdoviridae: nucleorhabdovirus).crops that are resistant to pests and pathogens are cost-effective for the management of pests and diseases. a corn (zea mays l.) breeding program conducted in hawaii has identified a source of heritable resistance to maize mosaic virus (mmv) (rhabdoviridae: nucleorhabdovirus). this resistance is controlled by the gene mv, which has been shown to have a codominant action. to date, no studies have examined whether the resistance associated with this gene affects only mmv or whether it also affect ...201324020306
infection rates and comparative population dynamics of peregrinus maidis (hemiptera: delphacidae) on corn plants with and without symptoms of maize mosaic virus (rhabdoviridae: nucleorhabdovirus) infection.we examined the population dynamics of the corn planthopper peregrinus maidis (ashmead) (hemiptera: delphacidae) throughout a cycle of corn (zea mays l.) production on plants with or without symptoms of maize mosaic virus (mmv) (rhabdoviridae: nucleorhabdovirus) infection. our results indicate that the timing of mmv plant infection greatly influenced the planthopper's host plant colonization patterns. corn plants that expressed symptoms of mmv infection early in the crop cycle (28 d after planti ...201324331606
analysis of acquisition and titer of maize mosaic rhabdovirus in its vector, peregrinus maidis (hemiptera: delphacidae).the corn planthopper, peregrinus maidis (ashmead) (hemiptera: delphacidae), transmits maize mosaic rhabdovirus (mmv), an important pathogen of maize and sorghum, in a persistent propagative manner. to better understand the vectorial capacity of p. maidis, we determined the efficiency of mmv acquisition by nymphal and adult stages, and characterized mmv titer through development. acquisition efficiency, i.e., proportion of insects that acquired the virus, was determined by reverse transcriptase p ...201628076276
diversity of planthoppers associated with the winter rice agroecosystems in southern yunnan, china.a field survey of the overwintering planthoppers (hemiptera: delphacidae) associated with the rice agroecosystems in southern yunnan was carried out during january-february in 2010 and 2011. 22 species of planthoppers were collected and identified, with one species representing the subfamily stenocraninae and the other 21 species in delphacinae. nycheuma cognatum (muir), peregrinus maidis (ashmead), and pseudosogata vatrenus (fennah) were new provincial records for yunnan. the pest species, soga ...201222958347
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