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molecular assay for the identification of setaria tundra.we report the molecular characterization of the amplification products obtained by specific pcr experiments aimed to identify the 5s ribosomal spacer of setaria tundra specimens isolated from roe deer (capreolus capreolus) in north italy, which represent the first record of the parasite in this country. three different fragments of approximately 400, 800, and 1200 bp (base pairs) are produced. sequence analyses showed that all three fragments share a very high level of similarity to 5s spacer se ...200314597287
the morphology of the filaroid nematode setaria tundra, the cause of peritonitis in reindeer rangifer tarandus.in finland during 2003-2005, large numbers of nematodes, tentatively identified as setaria sp., were observed in the peritoneal cavity of reindeer rangifer tarandus during a peritonitis outbreak associated with poor body condition. the morphology of worms recovered from slaughtered reindeer is described based on light and scanning electron microscopy. worms were confirmed as belonging to the superfamily filarioidea by the presence of microfilariae-producing females and the size and the shape of ...200717381867
outbreak of parasitic peritonitis in reindeer in finland.in 2003, there was an outbreak of peritonitis in reindeer in the southern and middle part of the finnish reindeer herding area caused by the filarioid nematode setaria species. in the province of oulu, the proportion of reindeer viscera condemned owing to parasitic lesions increased from 4.9 per cent in 2001 to 40.1 per cent in 2003. in 2004, the focus of the outbreak moved approximately 100 km north. a total of 260 adult and pre-adult setaria species nematodes were collected for morphological a ...200717575247
setaria tundra microfilariae in reindeer and other cervids in finland.harmful parasites of the wild northern boreal mammals are still surprisingly poorly studied. in 2003-2006, a peritonitis outbreak caused by the filarioid nematode, setaria tundra, emerged in finland's reindeer population. in order to gain knowledge about the basic biology, epidemiology, and transmission dynamics of this parasite, samples for s. tundra were collected from reindeer and other cervids during the follow-up period 2004-2006. using morphology and molecular biology methods, we describe ...200918810493
efficacy of different treatment regimes against setariosis (setaria tundra, nematoda: filarioidea) and associated peritonitis in reindeer.when a severe peritonitis outbreak in semi-domesticated reindeer was noticed in 2003 in finland, the concerned industry urged immediate preventive actions in order to avoid detrimental effects of s. tundra and further economical losses. a research programme was swiftly initiated to study s. tundra and its impact on the health and wellbeing of reindeer.200819087262
vectors and transmission dynamics for setaria tundra (filarioidea; onchocercidae), a parasite of reindeer in finland.abstract:200919126197
climate change promotes the emergence of serious disease outbreaks of filarioid nematodes.filarioid parasites represent major health hazards with important medical, veterinary, and economic implications, and considerable potential to affect the everyday lives of tens of millions of people globally (world health organization, 2007). scenarios for climate change vary latitudinally and regionally and involve direct and indirect linkages for increasing temperature and the dissemination, amplification, and invasiveness of vector-borne parasites. high latitude regions are especially influe ...201020422252
phylogenetic study of setaria cervi based on mitochondrial cox1 gene sequences.the objective of the present study was to examine the phylogenetic position of setaria cervi based on sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. a fragment of the cox1 gene from two morphologically identified s. cervi collected from red deer (cervus elaphus) from italy were amplified, sequenced, and compared with corresponding sequences of other filarioid nematode species. phylogenetic studies using bayesian analysis revealed s. cervi as monophyletic with other setari ...201121695569
molecular detection of dirofilaria immitis, dirofilaria repens and setaria tundra in mosquitoes from germany.as a result of globalization and climate change, dirofilaria immitis and dirofilaria repens, the causative agents of dirofilariosis in europe, continue to spread from endemic areas in the mediterranean to northern and northeastern regions of europe where autochthonous cases of dirofilarial infections have increasingly been observed in dogs and humans. whilst d. repens was recently reported from mosquitoes in putatively non-endemic areas, d. immitis has never been demonstrated in mosquitoes from ...201424433279
molecular detection of setaria tundra (nematoda: filarioidea) and an unidentified filarial species in mosquitoes in germany.knowledge of the potential vector role of culicidae mosquitoes in germany is very scanty, and until recently it was generally assumed that they are not involved in the transmission of anthroponotic or zoonotic pathogens in this country. however, anticipated changes in the course of global warming and globalization may alter their status.201222236560
screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria.both dirofilaria repens and recently d. immitis are known to be endemic in hungary. as one of several recent cases, the fatal case of a dog infested with d. immitis in szeged, southern hungary, received attention from the media. hence it was decided to catch mosquitoes in the garden where the dog lived to screen for filarioid helminths and plasmodium spp. using molecular tools.201525582219
first report of setaria tundra in roe deer (capreolus capreolus) from the iberian peninsula inferred from molecular data: epidemiological implications.filarioid nematode parasites are major health hazards with important medical, veterinary and economic implications. recently, they have been considered as indicators of climate change.201627682456
circulation of dirofilaria repens, setaria tundra, and onchocercidae species in hungary during the period 2011-2013.dirofilaria repens and recently dirofilaria immitis are known to be endemic in hungary. since there is no related research on dirofilaria carrier mosquito species from hungary, we conducted a three-year mosquito surveillance study between 2011 and 2013. during the study period we examined 23,139 female mosquitoes with a generic filaria-specific taqman pcr assay, and characterized them by sequencing a 500 bp segment of 12s rrna. an important result of our study was the detection of setaria tundra ...201526412142
is molecular xenomonitoring of mosquitoes for dirofilaria repens suitable for dirofilariosis surveillance in endemic regions?dirofilaria repens is a parasite of animals and humans, transferred by mosquitoes. the assessment of the presence of d. repens-infected vertebrate hosts in the investigated area can be performed by xenomonitoring—detection of the parasite in blood-feeding arthropods. our study aimed to evaluate pcr xenomonitoring of mosquitoes as a tool for dirofilariosis surveillance in poland. we were also interested whether inter-study comparisons at the international level would be possible. mosquitoes were ...201626490684
detection and molecular characterization of the mosquito-borne filarial nematode setaria tundra in danish roe deer (capreolus capreolus).setaria tundra is a mosquito-borne filarial nematode of cervids in europe. it has recently been associated with an emerging epidemic disease causing severe morbidity and mortality in reindeer and moose in finland. here, we present the first report of s. tundra in six roe deer (capreolus capreolus) collected between october 2010 and march 2014 in denmark. the deer originated from various localities across the country: the eastern part of the jutland peninsular and four locations on the island zea ...201728229043
molecular mass screening of mosquitoes for filarial parasites in germany--re-interpretation of pcr xenomonitoring results would be required.comments concerning interpretation of the pcr xenomonitoring results in the article "molecular detection of setaria tundra (nematoda: filarioidea) and an unidentified filarial species in mosquitoes in germany" parasites & vectors 2012, 5:14.201526646774
a lymphatic dwelling filarioid nematode, rumenfilaria andersoni (filarioidea; splendidofilariinae), is an emerging parasite in finnish cervids.recent studies revealed expansion of filarioid nematodes into northern finland. in addition to setaria tundra, an abundant filarioid, rumenfilaria andersoni, was found inhabiting the lymphatic vessels of reindeer. our study explores the dynamics of the rapid geographic expansion of r. andersoni, defining prevalence and density of microfilariae among 4 new cervid host species in finland while developing a context for host-parasite ecology in fennoscandia and more broadly in the arctic and boreal ...201525884201
setaria tundra in roe deer (capreolus capreolus)--new findings in poland.setaria tundra is a filarioid nematode transmitted by mosquitoes, which is sporadically observed in poland. some ecological factors, such as the synurbisation of some wild ungulates and the climate changes observed in recent years, could affect the possibility of infection, especially with parasites transmitted by arthropod vectors. the study was conducted to evaluate the presence of setaria sp. in roe deer populations in different environments. parasitological dissections of 53 roe deer were pe ...201324791344
defining parasite biodiversity at high latitudes of north america: new host and geographic records for onchocerca cervipedis (nematoda: onchocercidae) in moose and caribou.onchocerca cervipedis is a filarioid nematode of cervids reported from central america to boreal regions of north america. it is found primarily in subcutaneous tissues of the legs, and is more commonly known as 'legworm'. blackflies are intermediate hosts and transmit larvae to ungulates when they blood-feed. in this article we report the first records of o. cervipedis from high latitudes of north america and its occurrence in previously unrecognized host subspecies including the yukon-alaska m ...201223110962
the impact of global climate change on the spread of parasitic nematodesclimate changes may influence the frequency, intensity and geographical distribution of parasites, directly affecting their dispersive stages in the environment (eggs, larvae) and, indirectly, the larvae living mainly in invertebrate intermediate hosts. in biologically diverse nematodes climate warming contributes to the increase in the range of distribution, colonization of new hosts and modification of their development cycles. this is particularly acute in the arctic and pertains, for instanc ...201728432859
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