| [characterization of the biological activities of the 'yellow' and 'white' venoms from crotalus durissus ruruima compared with the crotalus durissus terrificus venom. neutralizing activity of crotalus durissus ruruima antivenins]. | the biological activities of 'yellow' and 'white' venom of a rattlesnake crotalus durissus ruruima hoge, 1965, found in the savanna-like vegetation (cerrado) of northern brazil (roraima) and venezuela have been studied, and compared to the reference crotalus durissus terrificus venom. the lethal activity of venoms depended on the inoculation route. the most toxic venom was the white one. the venoms of c. d. terrificus and the yellow of c. d. ruruima had similar lethalities. the yellow venom of c ... | 1993 | 8310446 |
| specific identification of lachesis muta muta snake venom using antibodies against the plasminogen activator enzyme, lv-pa. | sandwich-type enzyme linked immunosorbent assays (elisa) were developed to detect lachesis muta muta (bushmaster) snake venom using antibodies against the plasminogen activator enzyme (lv-pa). antibodies to lv-pa were obtained by immunization of one rabbit with the purified enzyme. the igg fraction was purified from rabbit blood in a single step on a column of sepharose-l. m. muta venom and used to coat the microtiter plates. the specificity of the assay was demonstrated by its capacity to corre ... | 2005 | 15804530 |
| enzymatic and structural characterization of new pla2 isoform isolated from white venom of crotalus durissus ruruima. | this work reports the structural and enzymatic characterization of a new spla2 from the white venom of crotalus durissus ruruima, nominated pla2a. the homogeneity of the pla2a fraction and its molecular mass were initially evaluated by sds-page and confirmed by maldi-tof spectrometry, indicating a molecular mass of 14,299.34da. structural investigation, through circular dichroism spectroscopy, revealed that pla2a has a high content of alpha helix and beta-turn structures, 45.7% and 35.6% respect ... | 2009 | 19013478 |
| neuromuscular effects of venoms and crotoxin-like proteins from crotalus durissus ruruima and crotalus durissus cumanensis. | a myographic study was performed to compare the neuromuscular effects of venoms and crotoxin-like proteins from crotalus durissus ruruima and crotalus durissus cumanensis in mice phrenic-diaphragm preparation. it was concluded that both venoms present neurotoxic activity as a consequence of their crotoxin content. furthermore, crotoxin from c.d. cumanensis is more potent than that from c.d. ruruima venom. at the concentration range in which both venoms express neurotoxic activity, only c.d. cuma ... | 2015 | 25598498 |
| biochemical, pharmacological and structural characterization of two pla2 isoforms cdr-12 and cdr-13 from crotalus durissus ruruima snake venom. | cdr-12 and cdr-13 isoforms of pla2, a d49 protein, were purified from crotalus durissus ruruima venom after one chromatographic step, reverse phase hplc on micro-bondapack c-18. the molecular mass by sds-page of cdr-12 and cdr-13 isoforms of pla2 was 14333.49 da and 14296.42 da, respectively and confirmed by maldi-tof mass spectrometry. the amino acid composition showed that both isoforms cdr-12 and cdr-13 have a high content of lys, tyr, gly, arg, and 14 half-cys residues, typical of a basic pl ... | 2007 | 17203396 |
| individual venom variability in crotalus durissus ruruima snakes, a subspecies of crotalus durissus from the amazonian region. | venoms of six specimens of crotalus durissus ruruima snakes from the same geographical site in the brazilian state of roraima, were individually assayed for their main pharmacological properties. quantitative and qualitative differences were found and the presence of crotoxin-like isoforms in these venoms was indicated. our findings corroborate the existence of a considerable intrapopulational variability in c. d. ruruima venoms, and the importance of using a pool of venoms for antivenom product ... | 2005 | 16269162 |
| comparison of the effect of crotalus simus and crotalus durissus ruruima venoms on the equine antibody response towards bothrops asper venom: implications for the production of polyspecific snake antivenoms. | antivenoms are preparations of immunoglobulins purified from the plasma of animals immunized with snake venoms. depending on the number of venoms used during the immunization, antivenoms can be monospecific (if venom from a single species is used) or polyspecific (if venoms from several species are used). in turn, polyspecific antivenoms can be prepared by purifying antibodies from the plasma of animals immunized with a mixture of venoms, or by mixing antibodies purified from the plasma of anima ... | 2010 | 21130107 |
| effect of the synthetic coumarin, ethyl 2-oxo-2h-chromene-3-carboxylate, on activity of crotalus durissus ruruima spla2 as well as on edema and platelet aggregation induced by this factor. | we show that ethyl 2-oxo-2h-chromene-3-carboxylate (eocc), a synthetic coumarin, irreversibly inhibits phospholipase a(2) (spla2) from crotalus durissus ruruima venom (spla2r) with an ic(50) of 3.1 +/- 0.06 nmol. eocc strongly decreased the v(max) and k(m), and it virtually abolished the enzyme activity of spla2r as well as spla2s from other sources. the edema induced by spla2r + eocc was less than that induced by spla2r treated with p-bromophenacyl bromide, which was more efficient at neutraliz ... | 2010 | 20223257 |
| biochemical and biological characterization of a pla2 from crotoxin complex of crotalus durissus cumanensis. | a new pla2 (cdcum6) from crotoxin complex of colombian crotalus durissus cumanensis rattlesnake was purified using molecular exclusion chromatography and rp-hplc. the molecular mass of cdcum6 was determined by sds-page approximately 14 kda and confirmed by maldi-tof (14321.98 da). the enzyme showed km 6.0 mm, vmax 3.44 nmol/min, optimum ph was 8.0 and temperature was between 30 and 45 degrees c, and it had a strict requirement of ca2+ for its activity. the n-terminal sequence of pla2 was slvqf e ... | 2009 | 19673100 |