| isolation and characterization of a convulxin-like protein from crotalus durissus collilineatus venom. | a convulxin (cvx)-like protein was isolated from crotalus durissus collilineatus venom by a combination of molecular exclusion and reversed-phase hplc chromatographies. the molecular mass of the cvx-like protein in the absence and presence of dtt was 78 kda and 12-13 kda, respectively. the cvx-like protein consisted of two nonidentical polypeptide chains (alpha and beta). the n-terminal amino-acid sequences of the alpha and beta subunits were glhcpsdwyaydghcykifneemnwed and gfccpshwssysrycykffsq ... | 2001 | 11838547 |
| isolation and preliminary enzymatic characterization of a novel pla2 from crotalus durissus collilineatus venom. | a crotoxin homolog was purified from the crotalus durissus collilineatus venom using molecular exclusion and reverse-phase hplc. this crotoxin contained one pla2 (cdcolli iii f6) and four crotapotin isoforms, whereas crotoxin from crotalus durissus terrificus venom had three pla2 iso forms and two crotapotin isoforms. sds-page showed that the c. d. collilineatus pla2 and crotapotin had relative molecular mass of 15 and 9 kda, respectively. neither the pla2 (cdcolli iii f6) nor the crotapotins (c ... | 2002 | 12018613 |
| isolation and biochemical characterization of a galactoside binding lectin from bauhinia variegata candida (bvcl) seeds. | a new lectin (bvcl) from seeds of a primitive brazilian caesalpinoideae, the bauhinia variegata candida was purified and biochemical characterized. bvcl was isolated by gel filtration chromatography on sephadex g75 and affinity chromatography on immobilized d: -lactose column. sds-page showed that bvcl under non-reducing condition presents two bands of 68 and 32 kda and a single band of 32 kda in reducing condition. however, only one band was seen in native page. the hemagglutination activity of ... | 2007 | 17203390 |
| isolation of a crotoxin-like protein from the venom of a south american rattlesnake (crotalus durissus collilineatus). | 1. a crotoxin-like protein was isolated from the venom of a south american rattlesnake crotalus durissus collilineatus. 2. many of its properties are similar to those of crotoxin, including its non-covalent heterodimeric structure, electrophoretic mobility on sds-page, isoelectric focusing properties, toxicity in mice, immunological reactivity, multiple isoforms, phospholipase activity, peptide map, and instability on an anion-exchange column. 3. results indicate that "collilineatus toxin" is st ... | 1990 | 2085953 |