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real-time fluorescent polymerase chain reaction detection of phytophthora ramorum and phytophthora pseudosyringae using mitochondrial gene regions.abstract a real-time fluorescent polymerase chain reaction (pcr) detection method for the sudden oak death pathogen phytophthora ramorum was developed based on mitochondrial dna sequence with an abi prism 7700 (taqman) sequence detection system. primers and probes were also developed for detecting p. pseudosyringae, a newly described species that causes symptoms similar to p. ramorum on certain hosts. the species-specific primer-probe systems were combined in a multiplex assay with a plant prime ...200618943415
phytophthora pseudosyringae sp. nov., a new species causing root and collar rot of deciduous tree species in europe.in several studies of oak decline in europe, a semi-papillate homothallic phytophthora taxon was consistently isolated, together with other phytophthora species, from rhizosphere soil samples. it was also found associated with necrotic fine roots and stem necroses of fagus sylvatica and alnus glutinosa. due to morphological and physiological similarities, the semi-papillate isolates were previously identified as p. syringae by various authors. the morphology, physiology and pathogenicity against ...200312967204
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