| genetic variability of begomoviruses associated with cotton leaf curl disease originating from india. | cotton leaf curl disease (clcud) causing viruses belong to the begomovirus genus of the family geminiviridae. most begomoviruses are bipartite with two molecules of circular single stranded dna (a and b) encapsidated in icosahedral geminate particles. however, the begomoviruses associated with clcud have dna-beta instead of dna-b. in this communication we report the complete genomic sequence of dna-a component of two clcud-causing begomoviruses, cotton leaf curl kokhran virus-dabawali (clcukv-da ... | 2004 | 15669112 |
| nucleotide sequencing, whitefly transmission, and screening tomato for resistance against two newly described begomoviruses in bangladesh. | abstract the molecular diversity of tomato leaf curl viruses (tolcvs), from the two main tomato growing areas of jessore and joydebpur, bangladesh, was investigated. the viral dna was amplified from tomato plants exhibiting mild and severe symptoms by polymerase chain reaction, and the complete genomes of the tolcvs were sequenced. an isolate of the bipartite tomato leaf curl new delhi virus-severe (tolcndv-svr) was associated with the severe symptom phenotype from jessore (tolcndv-svr[jes]). a ... | 2005 | 18943559 |
| molecular characterization of a new species of begomovirus and betasatellite causing leaf curl disease of tomato in india. | a new tomato-infecting begomovirus and cognate betasatellite were characterized from the gangetic plain of northern india. genome organization of this virus was found to be similar to those of other old world begomoviruses. the dna-a molecule (2752nt) shared maximum (85.8%) identity with tomato leaf curl laos virus-[laos] (tolclv-[la]; af195782) from laos and betasatellite molecule (1349nt) shared maximum (75.8%) identity with tomato leaf curl joydebpur betasatellite (tolcjob-[bd:gaz:05]; aj9662 ... | 2010 | 20540978 |
| molecular characterization of tomato leaf curl china virus, infecting tomato plants in china, and functional analyses of its associated betasatellite. | a novel tomato-infecting begomovirus from guangxi province, china, was identified and characterized, for which the name tomato leaf curl china virus (tolccnv) was proposed. phylogenetic and recombination analyses of the virus genomic sequences suggested that tolccnv may have arisen by recombination among tomato leaf curl vietnam virus (tolcvv), tomato leaf curl gujarat virus (tolcgv), and an unknown virus. a betasatellite molecule was found to be associated with tolccnv (tolccnb), and its comple ... | 2011 | 21378048 |
| xanthium strumarium: a weed host of components of begomovirus-betasatellite complexes affecting crops. | xanthium strumarium is a common weed that often shows symptoms typical of begomovirus infection, such as leaf curling and vein thickening. the virus complex isolated from the weed consisted of two begomoviruses along with a betasatellite and an alphasatellite. the first begomovirus was shown to be an isolate of cotton leaf curl burewala virus, a new recombinant begomovirus species that is associated with resistance breaking in previously resistant cotton varieties in pakistan, whereas the second ... | 2011 | 21969121 |
| synergistic interaction among begomoviruses leads to the suppression of host defense-related gene expression and breakdown of resistance in chilli. | chilli (capsicum sp.) is one of the economically important spice and vegetable crops grown in india and suffers great losses due to the infection of begomoviruses. conventional breeding approaches have resulted in development of a few cultivars of chilli resistant to begomoviruses. a severe leaf curl disease was observed on one such resistant chilli cultivar (capsicum annuum cv. kalyanpur chanchal) grown in the experimental field of the jawaharlal nehru university, new delhi. four different vira ... | 2016 | 26780359 |
| biology and interactions of two distinct monopartite begomoviruses and betasatellites associated with radish leaf curl disease in india. | emerging whitefly transmitted begomoviruses are major pathogens of vegetable and fibre crops throughout the world, particularly in tropical and sub-tropical regions. mutation, pseudorecombination and recombination are driving forces for the emergence and evolution of new crop-infecting begomoviruses. leaf curl disease of field grown radish plants was noticed in varanasi and pataudi region of northern india. we have identified and characterized two distinct monopartite begomoviruses and associate ... | 2012 | 22339942 |
| predominance of tomato leaf curl gujarat virus as a monopartite begomovirus: association with tomato yellow leaf curl thailand betasatellite. | tomato leaf curl is a serious malady in the state of maharashtra, india, causing nearly 100 % yield loss. an extensive survey was done in the affected fields of tomato in the year 2008, and members of three species of begomoviruses were identified as causing the disease. more than 60 % of the samples from diseased plants were infected with tomato leaf curl gujarat virus (tolcguv). isolates collected from these fields differed from the varanasi isolate of tolcguv in not having a dna b component. ... | 2013 | 22983111 |
| differential pathogenicity among tomato leaf curl gujarat virus isolates from india. | tomato leaf curl gujarat virus (tolcgv) has been identified as one of the most destructive pathogens causing tomato leaf curl disease (tolcd) in india. in the tomato growing regions of dhanbad and ramgarh, plants bearing severe symptoms of tolcd such as leaf curling, leaf crinkling, yellowing and leaf rolling was observed in the farmer fields. the association of begomovirus in these samples was confirmed by pcr and the causal viruses were identified as the isolates of tolcgv. however, associatio ... | 2013 | 24026875 |
| host-specific adaptation of diverse betasatellites associated with distinct indian tomato-infecting begomoviruses. | tomato leaf curl viruses cause major crop loss hindering tomato cultivation worldwide. the 'old world' begomoviruses are often associated with circular ssdna satellite molecules called betasatellites. in the present study, replication compatibility of five different betasatellites with three distinct indian tomato-infecting begomoviruses representing each of a monopartite, a mono-bipartite and a bipartite begomoviruses was studied. all the betasatellites could be trans-replicated by the begomovi ... | 2014 | 24384936 |
| development of plants resistant to tomato geminiviruses using artificial trans-acting small interfering rna. | rna interference (rnai), a conserved rna-mediated gene regulatory mechanism in eukaryotes, plays an important role in plant growth and development, and as an antiviral defence system in plants. as a counter-strategy, plant viruses encode rnai suppressors to suppress the rnai pathways and consequently down-regulate plant defence. in geminiviruses, the proteins ac2, ac4 and av2 are known to act as rnai suppressors. in this study, we have designed a gene silencing vector using the features of trans ... | 2015 | 25512230 |
| a novel recombinant tomato-infecting begomovirus capable of transcomplementing heterologous dna-b components. | the genome of a tomato-infecting begomovirus from ranchi, india, was cloned, sequenced and analysed. the viral genome shared 88.3% sequence identity with an isolate belonging to the species tobacco curly shoot virus (tbcsv), and this virus should therefore be considered a member of a new species, tentatively named tomato leaf curl ranchi virus (tolcrnv). the dna-ß molecule, which had 74.5% sequence identity with tomato leaf curl bangladesh betasatellite (tolcbdb), is named tomato leaf curl ranch ... | 2011 | 21311922 |
| tomato leaf curl gujarat virus, a new begomovirus species causing a severe leaf curl disease of tomato in varanasi, india. | abstract the biological and molecular properties of tomato leaf curl gujarat virus from varanasi, india (tolcgv-[var]) were characterized. tolcgv-[var] could be transmitted by grafting and through whitefly transmission in a persistent manner. the full-length genome of dna-a and dna-b of tolcgv-[var] was cloned in puc18. sequence analysis revealed that dna-a (ay190290) is 2,757 bp and dna-b (ay190291) is 2,688 bp in length. tolcgv-[var] could infect and cause symptoms in tomato, pepper, nicotiana ... | 2003 | 18943612 |
| supervirulent pseudorecombination and asymmetric synergism between genomic components of two distinct species of begomovirus associated with severe tomato leaf curl disease in india. | isolates of two distinct begomovirus species, the severe strain of the species tomato leaf curl new delhi virus (tomato leaf curl new delhi virus-[india:new delhi:severe:1992]; tolcndv-[in:nd:svr:92], bipartite) and the varanasi strain of the species tomato leaf curl gujarat virus (tomato leaf curl gujarat virus-[india:varanasi:2001]; tolcgv-[in:var:01], mono/bipartite) infect tomato (lycopersicon esculentum) and cause severe yield losses in northern india. this study investigated the infectivit ... | 2008 | 18272774 |