| development of the micromonospora carbonacea var. africana atcc 39149 bacteriophage pmlp1 integrase for site-specific integration in micromonospora spp. | micromonospora carbonacea var. africana atcc 39149 contains a temperate bacteriophage, pmlp1, that is present both as a replicative element and integrated into the chromosome. sequence analysis of a 4.4 kb kpni fragment revealed pmlp1 att/int functions consisting of an integrase, an excisionase and the phage attachment site (attp). plasmids psprh840 and psprh910, containing the pmlp1 att/int region, were introduced into micromonospora spp. by conjugation from escherichia coli. sequence analysis ... | 2003 | 12949170 |