Publications

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molecular diversity of skin mucus lectins in fish.among lectins in the skin mucus of fish, primary structures of four different types of lectin have been determined. congerin from the conger eel conger myriaster and ajl-1 from the japanese eel anguilla japonica were identified as galectin, characterized by its specific binding to beta-galactoside. eel has additionally a unique lectin, ajl-2, which has a highly conserved sequence of c-type lectins but displays ca(2+)-independent activity. this is rational because the lectin exerts its function o ...200314662297
developmental and microbiological analysis of the inception of bioluminescent symbiosis in the marine fish nuchequula nuchalis (perciformes: leiognathidae).many marine fish harbor luminous bacteria as bioluminescent symbionts. despite the diversity, abundance, and ecological importance of these fish and their apparent dependence on luminous bacteria for survival and reproduction, little is known about developmental and microbiological events surrounding the inception of their symbioses. to gain insight on these issues, we examined wild-caught larvae of the leiognathid fish nuchequula nuchalis, a species that harbors photobacterium leiognathi as its ...200818978090
tandem repeat l-rhamnose-binding lectin from the skin mucus of ponyfish, leiognathus nuchalis.two lactose-binding lectins (pfl-1 and -2) were identified in the skin mucus of ponyfish, leiognathus nuchalis. the molecular masses of pfl-1 and -2 were estimated to be 24 and 30kda, respectively. cloning of the pfl-1 cdna demonstrated its unique tandem repeat structure composed of two homologous domains with 41.7% internal identity. furthermore, pfl-1 exhibited homology with l-rhamnose-binding lectins previously purified from the eggs of fish and sea urchins. the n-terminal amino acid sequence ...200515950177
development of genotoxicity assay systems that use aquatic organisms.our aim is to develop and evaluate monitoring systems that use aquatic organisms to assess the genotoxicity of water in the field and in the laboratory. in a field study, we have shown that the micronucleus assay is applicable to freshwater and marine fishes and that gill cells are more sensitive than hematopoietic cells to micronucleus-inducing agents. gill cells from carassius sp. (funa) and zacco platypus (oikawa) collected upstream on the tomio river (nara, japan), tended to have lower micro ...19989672655
vibrio sp. strain nm 10, isolated from the intestine of a japanese coastal fish, has an inhibitory effect against pasteurella piscicida.vibrio sp. strain nm 10 with an inhibitory activity against pasteurella piscicida k-iii was isolated from the intestine of a spotnape ponyfish (leiognathus nuchalis). this bacterium efficiently produced an antibacterial substance after growth at 20 degrees c for 24 h on 1/5 pybg agar prepared with 50% seawater at phs of 7.5 to 9.0. the antibacterial substance was heat labile and proteinaceous, with a molecular mass of less than 5 kda, possibly a bacteriocin or a bacteriocin-like substance.19979406423
osmotic control of luminescence and growth in photobacterium leiognathi from ponyfish light organs.osmolarity was found to control the luminescence and growth of photobacterium leiognathi strain ln-1a isolated from the light organ of the ponyfish leiognathus nuchalis (family leiognathidae). low osmolarity (ca. 400 mosm) stimulated luminescence per cell 80 to 100-fold to a level (ca. 2.0 x 10(4) quanta . s-1 . cell-1) equal to that of bacteria taken directly from the light organ and increased the level of luciferase per cell 8 to 10-fold compared to high osmolarity (ca. 800 mosm). conversely, ...19853994483
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