polystoma cuvieri n. sp. (monogenea: polystomatidae), a parasite of the urinary bladder of the leptodactylid frog physalaemus cuvieri in paraguay.a new species of polystomatid monogenean, polystoma cuvieri, is described from the urinary bladder of the leptodactylid frog physalaemus cuvieri fitzinger in paraguay. this new species possesses a reticulated intestine and hamuli of about 350 microns long. the closest species is polystoma napoensis vaucher, 1987, described from osteocephalus spp. in ecuador. the paraguayan material is distinguished by the blade size of the hamuli. the hamuli blades appear to be useful in polystoma systematics.19902380858
helminth parasite communities of two physalaemus cuvieri fitzinger, 1826 (anura: leiuperidae) populations under different conditions of habitat integrity in the atlantic rain forest of brazil.abstractadults of physalaemus cuvieri were collected and necropsied between november 2009 and january 2010. this was carried out in order to report and compare the helminth fauna associated with two populations of this anuran species from the brazilian atlantic rain forest under different conditions of habitat integrity. the hosts from the disturbed area were parasitized with five helminth taxa: cosmocerca parva, aplectanasp., physaloptera sp., rhabdias sp., oswaldocruzia subauricularis (nematod ...201526560662
helminth parasite communities of two physalaemus cuvieri fitzinger, 1826 (anura: leiuperidae) populations under different conditions of habitat integrity in the atlantic rain forest of brazil.adults of physalaemus cuvieri were collected and necropsied between november 2009 and january 2010. this was carried out in order to report and compare the helminth fauna associated with two populations of this anuran species from the brazilian atlantic rain forest under different conditions of habitat integrity. the hosts from the disturbed area were parasitized with five helminth taxa: cosmocerca parva, aplectana sp., physaloptera sp., rhabdias sp., oswaldocruzia subauricularis (nematoda) and ...201526675914
rapd variation and population genetic structure of physalaemus cuvieri (anura: leptodactylidae) in central brazil.studies about the organization of the genetic variability and population structure in natural populations are used either to understand microevolutionary processes or the effects of isolation by human-inducted landscape modifications. in this paper, we analyzed patterns of genetic population structure using 126 rapd loci scored for 214 individuals of physalaemus cuvieri, sampled from 18 local populations. around 97% of these loci were polymorphic. the among-population variation component (phi(st ...200617028961
diet of the colubrid snake, thamnodynastes strigatus (günther, 1858) from paraná state, brazil, with field notes on anuran predation.dissection of 44 specimens of the colubrid snake thamnodynastes strigatus (serpentes: colubridae) provided data on food habits. prey items include frogs (71.4% of the sample), rodents (14.3%), fishes (10.7%), and lizards (3.6%). the most of the anuran species recorded (bufo sp., leptodactylus sp., physalaemus cuvieri and scinax fuscovarius) are ground dwellers or call at the water level. field observations provided information on the feeding behavior.200011241971
monitoring the morphological integrity of neotropical anurans.amphibians are considered to be excellent bioindicators to their morphophysiological characteristics and life cycle. in this context, the present study investigated the morphological integrity of anuran larvae collected in preserved environments in the emas national park, in the municipality of mineiros (goiás state, brazil), and in environments representative of the agricultural matrix of the rio verde region, also in goiás, where there is a long history of the use of agricultural pesticides. s ...201930474816
unraveling the variability and genetic structure of barker frog physalaemus cuvieri (leiuperinae) populations from different regions of brazil.the barker frog physalaemus cuvieri is widely distributed in south america and is found in all regions of brazil. significant intraspecific morphological variation in this species has been reported. to determine the genetic structure of the natural brazilian populations of p. cuvieri, 10 different populations geographically separated by 99.41 to 2936.75 km were evaluated using 10 polymorphic microsatellite loci. in addition, mitochondrial dna data were analyzed to determine genetic distance betw ...201424634290
acoustic niche partitioning in an anuran community from the municipality of floriano, piauí, brazil.vocalizations are an important trait for the identification of cryptic and/or closely related amphibian species. different vocalizations also contribute to partitioning of the acoustic space by sympatric species. this study aimed to describe the advertisement calls of anurans in a pond of the municipality of floriano, state of piauí, brazil, and infer the acoustic niche partitioning of amphibians. euclidean distance was used in a cluster analysis approach to infer the acoustic similarities among ...201930365635
sub-lethal effects induced by a mixture of different pharmaceutical drugs in predicted environmentally relevant concentrations on lithobates catesbeianus (shaw, 1802) (anura, ranidae) tadpoles.the increasing consumption of medications by humans has negative effects such as the increased disposal of these compounds in the environment. little is known about how the disposal of a "drug mix" (dm) in aquatic ecosystems can affect their biota. thus, we evaluated whether the exposure of lithobates casteibeianus tadpoles to a dm composed of different medication classes (antibiotic, anti-inflammatory, antidepressant, anxiolytic, analgesic, and antacid drugs)-at environmentally relevant concent ...201930411290
study of closely related species within the physalaemus cuvieri group (anura): contribution of microsatellite markers.various species of the physalaemus cuvieri group of frogs are difficult to distinguish morphologically, making molecular analysis an attractive alternative for indentifying members of this group, which is considered to be at risk because of loss of habitat. the genetic structure of natural populations of p. ephippifer and p. albonotatus species was investigated and analyzed, together with that of five previously studied populations of p. cuvieri. nine microsatellite loci were used in the analyse ...201121823093
phylogeny of frogs from the genus physalaemus (anura, leptodactylidae) inferred from mitochondrial and nuclear gene sequences.although some species groups have been recognized in the leiuperine genus physalaemus, no phylogenetic analysis has previously been performed. here, we provide a phylogenetic study based on mitochondrial and nuclear dna sequences from 41 of the 46 species of physalaemus. we employed the parsimony criterion using the software tnt and poy and the bayesian criterion using the software mrbayes. two major clades were recovered inside the monophyletic physalaemus: (i) the highly supported physalaemus ...201526143292
chromosomal analysis of physalaemus kroyeri and physalaemus cicada (anura, leptodactylidae).all the species of physalaemus fitzinger, 1826 karyotyped up until now have been classified as 2n = 22. the species of the physalaemus cuvieri group analyzed by c-banding present a block of heterochromatin in the interstitial region of the short arm of pair 5. physalaemus cicada bokermann, 1966 has been considered to be a member of the physalaemus cuvieri species group, although its interspecific phylogenetic relationships remain unknown. the pcp190 satellite dna has been mapped on the chromosom ...201627551351
impacts of tannery effluent on development and morphological characters in a neotropical tadpole.previous studies have shown the contamination of rivers and streams caused by tannery effluents (te). however, the effects of te on amphibians are still unknown. thus, we evaluated whether the te, even at low concentrations (0.2%), could cause asymmetric development (inferred through the fluctuating asymmetry indices (fa)) and changes in morphological characteristics of physalaemus cuvieri larvae. accordingly, the tadpoles were distributed in groups non-exposed (n=160) and exposed (n=160) to thi ...028648371
are oral deformities in tadpoles accurate indicators of anuran chytridiomycosis?we evaluated the use of oral deformities as reliable proxies for determining batrachochytrium dendrobatidis (bd) infection in tadpoles of six anuran species of the atlantic forest in southeastern brazil. we examined oral discs of 2156 tadpoles of six species of anurans collected in 2016: aplastodiscus albosignatus, boana albopunctata, boana faber, scinax hayii, crossodactylus caramaschii, and physalaemus cuvieri. three oral deformities were recognized: lack of keratinization only in upper and/or ...029320551
polytypic and polymorphic cytogenetic variations in the widespread anuran physalaemus cuvieri (anura, leiuperidae) with emphasis on nucleolar organizing regions.we investigated the nor distribution in ten populations of physalaemus cuvieri from different regions of brazil and argentina. a high variability in nor pattern was observed and provided a useful tool in grouping several populations. the specimens from the state of tocantins, northern brazil, could easily be distinguished from all the other analyzed populations, since its karyotype presented nors in the chromosome pairs 1,3,4 and 10 (and sometimes also in chromosome 5), and several pericentromer ...200919621135
the pioneering use of issr (inter simple sequence repeat) in neotropical anurans: preliminary assessment of genetic diversity in populations of physalaemus cuvieri (amphibia, leiuperidae).the greatest diversity of anurans in the world is in brazil and one of the major challenges is to reconcile the accelerated economic development with strategies that aim to maintain this diversity in forest fragments, often representing esus of some biomes. this study aimed to obtain data that will support conservation projects through the pioneering use of issr analysis in neotropical anurans, estimating the intra- and interpopulation genetic diversity of four populations of p. cuvieri (paraná ...201323760415
the tadpole of physalaemus orophilus from the atlantic rainforest of southeastern brazil (amphibia, anura, leptodactylidae).the neotropical genus physalaemus fitzinger is currently composed by 48 species (cardozo pereyra 2018). recently, a phylogenetic analysis aiming to investigate the internal relationships of the genus recovered two major clades, the physalaemus cuvieri and p. signifer clades (lourenço et al. 2015). the following species groups were retrieved in the first clade: p. biligonigerus, p. cuvieri, p. gracilis, p. henselii, and p. olfersii groups (lourenço et al. 2015). this proposal redefined the p. olf ...201931712540
the tadpole of physalaemus nanus (boulenger, 1888) (anura, leptodactylidae) from southern brazil.the genus physalaemus fitzinger, 1826 currently comprises 47 described species distributed from central america (guianas) to uruguay (frost 2016). the genus has been recently organized in two main clades based on molecular data, physalaemus signifer clade and physalaemus cuvieri clade (lourenço et al. 2015). the p. signifer clade, with 15 species, is composed by the p. deimaticus and p. signifier groups, plus p. nattereri (steindachner, 1863) and p. maculiventris (lutz, 1925) (nascimento et al. ...201730308653
helminths associated with three physalaemus species (anura: leptodactylidae) from caatinga biome, brazil.knowledge of which and how many species are part of an ecosystem is fundamental to understanding the diversity of organisms, including parasitic organisms that vary widely among host populations. this study describes the composition, infection patterns, and similarity in composition of helminths associated with p. albifrons, p. cicada, and p. cuvieri.201930644061
atlantic amphibians: a data set of amphibian communities from the atlantic forests of south america.amphibians are among the most threatened vertebrates in the world and this is also true for those inhabiting the atlantic forest hotspot, living in ecosystems that are highly degraded and threatened by anthropogenic activities. we present a data set containing information about amphibian communities sampled throughout the atlantic forest biome in south america. the data were extracted from 389 bibliographic references (articles, books, theses, and dissertations) representing inventories of amphi ...201829953585
idiosyncratic liver pigment alterations of five frog species in response to contrasting land use patterns in the brazilian cerrado.changes in land use trigger environmental changes that can lead to decreased biodiversity and species loss. the liver is an essential detoxification organ that reflects systemic physiological responses to environmental changes. here, we tested whether contrasting land use patterns influence the amount of substances from the hepatic cellular catabolism and melanomacrophages (mms) of five anuran species in the brazilian cerrado.202032913675
morphological responses of three persistent native anuran species after forest conversion into monoculture pine plantations: tolerance or prosperity?species loss by habitat replacement operating as an ecological filter is a well-known consequence of modern human activities. in contrast, the ecological and evolutionary response of species overcoming those filters in converted habitats has not been thoroughly explored. species that persist are subject to novel and potentially stressful conditions that may induce certain morphological changes. we evaluated changes in the functional morphology of three anuran species persisting after the convers ...202032297462
acute toxicity of inorganic nitrogen (ammonium, nitrate and nitrite) to tadpoles of five tropical amphibian species.despite the higher diversity of amphibians and the increasing use of agrochemicals in tropical countries, knowledge on the ecotoxicity of such compounds to tropical amphibians remains very limited. the aim of this study was, therefore, to assess the acute lethal toxicity of three nitrogen salts (ammonium sulphate, sodium nitrate and sodium nitrite) to tadpoles of five tropical frog species: rhinella ornata, boana faber, b. pardalis, physalaemus cuvieri, and p. olfersii. the order of sensitivity ...202032638180
microplastic ingestion induces behavioral disorders in mice: a preliminary study on the trophic transfer effects via tadpoles and this study, the hypothesis that polyethylene microplastics (mps) can accumulate in animals, reach the upper trophic level and trigger behavioral changes was tested. physalaemus cuvieri tadpoles were exposed to mps (for 7 days) and fed on tambatinga fish for the same period. subsequently, these fish were given as food to swiss mice. the mp amount in animals' liver was quantified and results have evidenced its accumulation at all assessed trophic levels [tadpole: 18,201.9 particles/g; fish: 1.2 ...202132629346
nucleolus organizer regions in physalaemus cuvievi (anura, leptodactylidae), with evidence of a unique case of ag-nor variability.we studied ten specimens of physalaemus cuvieri collected at different localities in brazil using conventional staining and banding techniques. all specimens had 2n = 22. there were karyotypic variants: distinct patterns in the number and chromosome localization of ag-nors as well as in the corresponding secondary constrictions. preliminary c-banding patterns obtained for specimens from two localities are also suggestive of karyotypic differentiation in p. cuvieri.199910712096
satellite dna derived from 5s rdna in physalaemus cuvieri (anura, leiuperidae).in the present study, we describe for the first time a family of 190-bp satellite dna related to 5s rdna in anurans and the existence of 2 forms of 5s rdna, type i (201 bp) and type ii (690 bp). the sequences were obtained from genomic dna of physalaemus cuvieri from palmeiras, state of bahia, brazil. analysis of the nucleotide sequence revealed that the satellite dna obtained by digestion with ecori, called pcp190ecori, is 70% similar to the coding region of type i 5s rdna and 66% similar to th ...201121464559
helminth fauna of two species of physalaemus (anura: leiuperidae) from an undisturbed fragment of the atlantic rainforest, southeastern brazil.two amphibian species, physalaemus cuvieri and physalaemus olfersii, from serra do mar state park, which is an old-growth environment of the atlantic rainforest in southeastern brazil, were surveyed for endoparasites. hosts were sampled in 2 ponds; each was colonized by only 1 physalaemus species. the overall prevalence of helminths was high and similar in both amphibian species. the mean intensity of infection in p. olfersii did not differ statistically from that in p. cuvieri . nine helminth s ...201323409941
a persistent organic pollutant related with unusual high frequency of hermaphroditism in the neotropical anuran physalaemus cuvieri fitzinger, 1826.representing a reflection of anthropic activity, the level of xenobiotic compounds in aquatic ecosystems has increased in recent years, bringing severe damage to the environment. the present work reports the occurrence of malformation in gonads of physalaemus cuvieri individuals from a population of atlantic forest in southern brazil. twenty male specimens were collected, which had their testicles removed, immersed in karnovsky fixative solution, included in historesin for 2 μm cuts and stained ...201424742721
comparative cytogenetics of physalaemus albifrons and physalaemus cuvieri species groups (anura, leptodactylidae).recently, physalaemus albifrons (spix, 1824) was relocated from the physalaemus cuvieri group to the same group as physalaemus biligonigerus (cope, 1861), physalaemus marmoratus (reinhardt & lütken, 1862) and physalaemus santafecinus barrio, 1965. to contribute to the analysis of this proposition, we studied the karyotypes of physalaemus albifrons, physalaemus santafecinus and three species of the physalaemus cuvieri group. the karyotype of physalaemus santafecinus was found to be very similar t ...201425147623
long-time evolution and highly dynamic satellite dna in leptodactylid and hylodid frogs.satellite dna sequences are the most abundant components of heterochromatin and are repeated in tandem hundreds to thousands of times in the genome. however, the number of repeats of a specific satellite family can vary even between the genomes of related species or populations. the pcp190 satellite dna family was identified in the genome of the leptodactylid frog physalaemus cuvieri, which showed to be derived most likely from the 5s rdna in an ancestral species. in this study, we investigate t ...201425316286
can short exposure to polyethylene microplastics change tadpoles' behavior? a study conducted with neotropical tadpole species belonging to order anura (physalaemus cuvieri).the scientific knowledge about toxicological impacts of polyethylene microplastics (pe mps) on different organisms has significantly improved in recent years. however, the effects of these pollutants on animal species such as amphibians remain poorly known. thus, the aim of the current study is to investigate whether the short exposure (7 days) of physalaemus cuvieri tadpoles to pe mps (60 mg/l) can change their behavior. collected data have shown that pe mp accumulation in tadpoles was associat ...202032044637
hepatotoxicity of pristine polyethylene microplastics in neotropical physalaemus cuvieri tadpoles (fitzinger, 1826).plastic waste disposal in the environment is a major issue worldwide, whose effects on different biotas are the object of several investigations. the toxicity caused by microplastics (mps) in organisms living in freshwater environments remains little explored. little is known about the consequences of the exposure to these pollutants on the health of amphibians. thus, we tested the hypothesis that the exposure of physalaemus cuvieri tadpoles to microplastic polyethylene (pe mp) causes histopatho ...202031901713
how much are microplastics harmful to the health of amphibians? a study with pristine polyethylene microplastics and physalaemus cuvieri.microplastics (mps) are critical emerging pollutants found in the environment worldwide; however, its toxicity in aquatic in amphibians, is poorly known. thus, the aim of the present study is to assess the toxicological potential of polyethylene microplastics (pe mps) in physalaemus cuvieri tadpoles. according to the results, tadpoles' exposure to mp pe at concentration 60 mg/l for 7 days led to mutagenic effects, which were evidenced by the increased number of abnormalities observed in nuclear ...202031473515
lethal toxicity of the herbicides acetochlor, ametryn, glyphosate and metribuzin to tropical frog larvae.despite the high amphibian biodiversity and increasing pesticide use in tropical countries, knowledge on the sensitivity of tropical amphibians to pesticides remains limited. the aim of this study was to evaluate the acute toxicity of the active ingredients of four of the main herbicides used in brazilian sugarcane production to tadpoles of two tropical frog species: physalaemus cuvieri and hypsiboas pardalis. the calculated 96 h-lc50 (median lethal concentration; in mg a.s./l) values for p. cuv ...201931250286
diversification of the widespread neotropical frog physalaemus cuvieri in response to neogene-quaternary geological events and climate we reconstructed the demographical history and the dispersal dynamics of physalemus cuvieri through the neogene-quaternary periods by coupling dna regions with different mutation rates, ecological niche modelling, reconstruction of spatio-temporal lineage dispersal and coalescent simulations. still, to test alternative diversification scenarios we used approximate bayesian computation. molecular phylogenetic analysis recovered four deep and strongly supported clades, which we interpret as p ...201930508632
can environmental concentrations of glyphosate affect survival and cause malformation in amphibians? effects from a glyphosate-based herbicide on physalaemus cuvieri and p. gracilis (anura: leptodactylidae).herbicides are the most common agrochemicals used in crops. among them, glyphosate is the most widely applied in the world. herbicides, especially organophosphates, have been shown to be hazardous to non-target species, including amphibians. the present study evaluated the acute and chronic effects of glyphosate-based herbicide (gbh), roundup original® di on tadpoles from two south american native species, physalaemus cuvieri and p. gracilis. spawnings were collected in the natural environment a ...202032319061
effects of benzo[a]pyrene on the blood and liver of physalaemus cuvieri and leptodactylus fuscus (anura: leptodactylidae).benzo[a]pyrene (bap) is a bio-accumulative toxic compound found in the atmosphere, water, and soil that may affect the life cycle of amphibians. in this study, a few contamination biomarkers, such as hepatic melanomacrophages (mms), mast cells, erythrocyte micronuclei (mn) and white blood cells were used to determine how bap acts in these cells in the anurans physalaemus cuvieri and leptodactylus fuscus. animals of both species were divided into three treatment groups: 1 day, 7 days and 13 days, ...201829477119
genotoxic effects of glyphosate on physalaemus tadpoles.genotoxicity studies have revealed that pesticides bind to genetic material in non-target vertebrates, thereby impairing the genetic integrity of these animals. the main objective of this study was to determine the genotoxic damage in erythrocytes of two native south american amphibian physalaemus cuvieri and physalaemus gracilis, both species exposed to a glyphosate-based herbicide. we evaluated the presence of micronuclei (mn) and erythrocyte nuclear abnormalities (ena) as biomarkers for poten ...202033080355
acute and chronic toxicity of pesticides on tadpoles of physalaemus cuvieri (anura, leptodactylidae).brazil is the largest consumer of pesticides in the world. however, knowledge on how these pesticides affect wildlife is scarce. among the vertebrates, amphibians are particularly important in research to assess the impact of pesticides because of the correlation between pesticide and the decline of these species. this study aimed to evaluate the acute and chronic toxicity of commercial formulations of pesticides, i.e., atrazine (herbicide), cypermethrin (insecticide), and tebuconazole (fungicid ...201829397473
assessing genotoxicity and mutagenicity of three common amphibian species inhabiting agroecosystem environment.amphibians are constantly exposed to pollutants and the stress of agricultural activities. we selected three anuran amphibian species dendropsophus minutus, boana albopunctata, and physalaemus cuvieri, totaling 309 individuals. we collected tadpoles in 15 permanent ponds: 5 soybean crops, 3 corn crops, and 7 nonagricultural lands. our study provides the first comparative data on the genotoxicity and mutagenicity of three common amphibian anurans. dendropsophus minutus was the most vulnerable spe ...201931236619
genotoxic evaluation in tadpoles associated with agriculture in the central cerrado, brazil.many agricultural practices cause environmental degradation that affects the cellular integrity of anurans. in the present study, we provided in situ data of dendropsophus minutus, physalaemus cuvieri, and scinax fuscovarius collected in soybean/corn and conservation units in the brazilian cerrado. the in situ data showed no significant variation in the micronucleus frequency between the sites, only the reniform cells had a higher rate for the agricultural environment. a combined analysis of all ...201930949743
extensive cryptic diversity within the physalaemus cuvieri-physalaemus ephippifer species complex (amphibia, anura) revealed by cytogenetic, mitochondrial, and genomic markers.previous cytogenetic and phylogenetic analyses showed a high variability in the frog taxa physalaemus cuvieri and physalaemus ephippifer and suggested the presence of undescribed diversity in this species complex. here, by 1) adding specimens from the brazilian amazon region, 2) employing sequence-based species delimitation approaches, and 3) including radseq-style markers, we demonstrate that the diversity in the p. cuvieri-p. ephippifer species complex is even greater than previously suspected ...201931475033
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