| culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients. | renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. in this study, a culture-independent 16s rrna-based approach was established to identify unusual, fastidious, or anaerobic bacteria and to investigate bacterial diversity in urinary tract specimens. similarly sized amplicons encompassing the v6 to v8 region of the 16s rrna were analyzed with denaturing ... | 2003 | 14662931 |
| uncultivated phylotypes and newly named species associated with primary and persistent endodontic infections. | endodontic infections have been traditionally studied by culture methods, but recent reports showing that over 50% of the oral microbiota is still uncultivable (b. j. paster et al., j. bacteriol. 183:3770-3783, 2001) raise the possibility that many endodontic pathogens remain unknown. this study intended to investigate the prevalence of several uncultivated oral phylotypes, as well as newly named species in primary or persistent endodontic infections associated with chronic periradicular disease ... | 2005 | 16000454 |
| characterization of dialister species in infected root canals. | members of the dialister genus are asaccharolytic obligately anaerobic gram-negative coccobacilli that are culture-difficult or remain uncultivated. their participation in endodontic infections has been only consistently demonstrated after advent of molecular biology approaches. this study was undertaken to characterize dialister species in samples from primary endodontic infections using a devised 16s rrna gene-based group-specific heminested pcr assay followed by sequencing of pcr products. ge ... | 2006 | 17055906 |
| prevalence of selected bacterial named species and uncultivated phylotypes in endodontic abscesses from two geographic locations. | evidence is mounting that the prevalence of some putative endodontic pathogens can significantly vary according to the geographic location in which samples were taken. this study aimed to provide additional knowledge on this subject by comparing the frequencies of 10 selected candidate endodontic pathogens in samples of acute apical abscesses obtained from two distinct geographic locations; portland, or, and rio de janeiro, brazil. dna was extracted from aspirates and used as template in nested ... | 2006 | 17174667 |
| bacterial examination of endodontic infections by clonal analysis in concert with denaturing high-performance liquid chromatography. | the aim of this study was to examine the diversity of bacterial species in the infected root canals of teeth associated with endodontic abscesses by cloning and sequencing techniques in concert with denaturing high-performance liquid chromatography. | 2007 | 17949344 |
| microorganisms in root canal-treated teeth from a german population. | posttreatment apical periodontitis is usually associated with persistent or secondary intraradicular infection. this study evaluated the presence and relative levels of 28 bacterial taxa in treated root canals of teeth with posttreatment apical periodontitis from german patients using 16s ribosomal rna (rrna) gene probes in a reverse-capture checkerboard hybridization assay. species-specific polymerase chain reaction (pcr) was also performed to detect enterococcus faecalis and candida albicans. ... | 2008 | 18634922 |
| new bacterial compositions in root-filled teeth with periradicular lesions. | the aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. specimens were sampled from patients undergoing root canal retreatment. the bacteria were characterized by morphologic and biochemical analysis and by 16s rrna gene sequencing. microorganisms were detected in 10 of 18 teeth. the majority of positive samples revealed a mixed culture of 2-8 species. in 2 teeth enterococcus faecalis was the only detected species. for the first ... | 2009 | 19166766 |
| dysbiosis of the faecal microbiota in patients with crohn's disease and their unaffected relatives. | a general dysbiosis of the intestinal microbiota has been established in patients with crohn's disease (cd), but a systematic characterisation of this dysbiosis is lacking. therefore the composition of the predominant faecal microbiota of patients with cd was studied in comparison with the predominant composition in unaffected controls. whether dysbiosis is present in relatives of patients cd was also examined. | 2011 | 21209126 |
| viral-bacterial associations in acute apical abscesses. | objective: viral-bacterial and bacterial synergism have been suggested to contribute to the pathogenesis of several human diseases. this study sought to investigate the possible associations between 9 candidate endodontic bacterial pathogens and 9 human viruses in samples from acute apical abscesses. study design: dna extracts from purulent exudate aspirates of 33 cases of acute apical abscess were surveyed for the presence of 9 selected bacterial species using a 16s ribosomal rna gene-based nes ... | 2011 | 21507684 |
| analysis of symptomatic and asymptomatic primary root canal infections in adult norwegian patients. | this molecular study analyzed the microbiota of primary root canal infections from adult norwegian patients. | 2011 | 21846535 |
| new bacterial composition in primary and persistent/secondary endodontic infections with respect to clinical and radiographic findings. | the aim of the present study was to analyze the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings. | 2014 | 24767562 |
| bacteria in the apical root canals of teeth with apical periodontitis. | bacteria in the tooth root canal may cause apical periodontitis. this study examined the bacterial species present in the apical root canal of teeth with apical periodontitis. antibiotic sensitivity tests were performed to evaluate whether these identified bacterial species were susceptible to specific kinds of antibiotics. | 2016 | 27745799 |
| association between tobacco use and the upper gastrointestinal microbiome among chinese men. | tobacco causes many adverse health conditions and may alter the upper gastrointestinal (ugi) microbiome. however, the few studies that studied the association between tobacco use and the microbiome were small and underpowered. therefore, we investigated the association between tobacco use and the ugi microbiome in chinese men. | 2015 | 25701246 |
| antibiotic resistance genes in anaerobic bacteria isolated from primary dental root canal infections. | fourty-one bacterial strains isolated from infected dental root canals and identified by 16s rrna gene sequence were screened for the presence of 14 genes encoding resistance to beta-lactams, tetracycline and macrolides. thirteen isolates (32%) were positive for at least one of the target antibiotic resistance genes. these strains carrying at least one antibiotic resistance gene belonged to 11 of the 26 (42%) infected root canals sampled. two of these positive cases had two strains carrying resi ... | 2012 | 23108290 |
| microbial diversity in failed endodontic root-filled teeth. | persistent/secondary infections of human root canals play an important role in the failure of endodontic treatment. this study used 16s rrna sequencing to assess microbial diversity in root-filled teeth associated with failed endodontic treatment. | 2012 | 22613548 |
| molecular analysis of 16s rrna genes identifies potentially periodontal pathogenic bacteria and archaea in the plaque of partially erupted third molars. | small subunit rrna sequencing and phylogenetic analysis were used to identify cultivable and uncultivable microorganisms present in the dental plaque of symptomatic and asymptomatic partially erupted third molars to determine the prevalence of putative periodontal pathogens in pericoronal sites. | 2012 | 22326171 |
| advanced caries microbiota in teeth with irreversible pulpitis. | bacterial taxa in the forefront of caries biofilms are candidate pathogens for irreversible pulpitis and are possibly the first ones to invade the pulp and initiate endodontic infection. this study examined the microbiota of the most advanced layers of dentinal caries in teeth with irreversible pulpitis. | 2015 | 26187422 |
| molecular identification of cultivable bacteria from infected root canals associated with acute apical abscess. | the objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification (16s rrna) of cultivable bacteria. two hundred and twenty strains isolated by culture from 20 root canals were subjected to dna extraction and amplification of the 16s rrna gene (pcr), followed by sequencing. the resulting nucleotide sequences were compared to the genbank database from the national center of biotechnology inform ... | 2017 | 27224567 |
| bacterial diversity of symptomatic primary endodontic infection by clonal analysis. | the aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. samples were collected from 10 patients and submitted to bacterial dna isolation, 16s rrna gene amplification, cloning, and sequencing. a bacterial genomic library was constructed and bacterial diversity was estimated. the mean number of taxa per canal was 15, ranging from 11 to 21. a total of 689 clones were analyzed and 76 phylotypes identified, of which 47 (61.84%) w ... | 2016 | 27737357 |
| association between intestinal permeability and faecal microbiota composition in italian children with beta cell autoimmunity at risk for type 1 diabetes. | pancreatic organ-specific autoimmunity in subjects at risk for type 1 diabetes (t1d) is associated with increased intestinal permeability and an aberrant gut microbiota, but these factors have not yet been simultaneously investigated in the same subjects. thus, the aim of this study was to assess both intestinal permeability and gut microbiota composition in an italian sample of children at risk for t1d. | 2016 | 26891226 |
| microbiological diversity of peri-implantitis biofilm by sanger sequencing. | to examine the microbial diversity associated with implants with or without peri-implantitis and to identify differences between the bacterial communities in these clinical conditions. | 2014 | 23845046 |
| Microbial community analysis and identification of alternative host-specific fecal indicators in fecal and river water samples using pyrosequencing. | It is important to know the comprehensive microbial communities of fecal pollution sources and receiving water bodies for microbial source tracking. Pyrosequencing targeting the V1-V3 hypervariable regions of the 16S rRNA gene was used to investigate the characteristics of bacterial and Bacteroidales communities in major fecal sources and river waters. Diversity analysis indicated that cow feces had the highest diversities in the bacterial and Bacteroidales group followed by the pig sample, with ... | 2011 | 21887641 |
| exploring bacterial diversity of endodontic microbiota by cloning and sequencing 16s rrna. | the characterization of microbial communities infecting the endodontic system in each clinical condition may help on the establishment of a correct prognosis and distinct strategies of treatment. the purpose of this study was to determine the bacterial diversity in primary endodontic infections by 16s ribosomal-rna (rrna) sequence analysis. | 2011 | 21689545 |
| comparison of the in vivo antimicrobial effectiveness of sodium hypochlorite and chlorhexidine used as root canal irrigants: a molecular microbiology study. | the purpose of this clinical study was to compare the antimicrobial effects of 2.5% sodium hypochlorite (naocl) and 0.12% chlorhexidine digluconate (chx) when used as irrigants during treatment of teeth with apical periodontitis. | 2011 | 21238793 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| the microbiota of acute apical abscesses. | as the breadth of bacterial diversity in the oral cavity has been deciphered by molecular studies, several newly identified species/phylotypes have emerged as potential pathogens. we hypothesized that many of these species/phylotypes could also be involved with the etiology of endodontic abscesses. abscess aspirates from 42 persons were analyzed for the presence of 81 species/phylotypes by means of a reverse-capture checkerboard hybridization assay. associations between the most frequently detec ... | 2009 | 19131319 |
| asaccharolytic anaerobic gram-negative coccobacilli (aagnc) isolated from infected root canals and periodontal pockets. | culture-difficult bacteria, including asaccharolytic anaerobic gram-negative coccobacilli (aagnc), may constitute a predominant group of organisms in oral sites. this study aimed to characterize phylogenetically 10 aagnc isolated from endodontic lesions and periodontal pockets. | 2006 | 16390338 |
| dialister micraerophilus sp. nov. and dialister propionicifaciens sp. nov., isolated from human clinical samples. | seventeen anaerobic, gram-negative, tiny coccobacilli were collected in france from various human clinical samples. biochemical analyses as well as molecular studies, including 16s rrna and dnak gene sequencing, affiliated all the isolates to the genus dialister. however, 16s rrna and dnak gene sequence similarities were below 95.2 and 79.7 %, respectively, when comparisons were performed with the currently described species dialister pneumosintes and dialister invisus. two clusters consisting o ... | 2005 | 16280512 |
| detection of novel oral species and phylotypes in symptomatic endodontic infections including abscesses. | this study was undertaken to investigate the occurrence of several uncultivated phylotypes and newly named bacterial species in symptomatic endodontic infections. samples taken from cases clinically diagnosed as acute periradicular abscesses or acute periradicular periodontitis were surveyed for the presence of 12 taxa by means of a 16s rrna-gene-based nested or hemi-nested pcr assay. the most prevalent of the target taxa were dialister invisus, olsenella uli, granulicatella adiacens, and synerg ... | 2005 | 16099112 |
| dialister invisus sp. nov., isolated from the human oral cavity. | six strains of anaerobic, gram-negative coccobacilli isolated from the root canals of patients with endodontic infections (five strains) and from a deep periodontal pocket (one strain) were subjected to a comprehensive range of phenotypic and genetic tests and were found to comprise a homogeneous group. following 16s rrna gene sequence analysis, they were found to be most closely related to dialister pneumosintes, with 93 % sequence similarity between the two taxa. a novel species, dialister inv ... | 2003 | 14657126 |