| prime-boost vaccination of mice and rhesus macaques with two novel adenovirus vectored covid-19 vaccine candidates. | abstractcovid-19 vaccines are being developed urgently worldwide. here, we constructed two adenovirus vectored covid-19 vaccine candidates of sad23l-ncov-s and ad49l-ncov-s carrying the full-length gene of sars-cov-2 spike protein. the immunogenicity of two vaccines was individually evaluated in mice. specific immune responses were observed by priming in a dose-dependent manner, and stronger responses were obtained by boosting. furthermore, five rhesus macaques were primed with 5 × 109 pfu sad23 ... | 2021 | 33993845 |
| generation of a novel replication-incompetent adenoviral vector derived from human adenovirus type 49: manufacture on per.c6 cells, tropism and immunogenicity. | recombinant adenoviral vectors based on type 5 (rad5) show great promise as a vaccine carrier. however, neutralizing activity against ad5 is prevalent and high-titred among human populations, and significantly dampens ad5-based vaccine modalities. the generation of alternative adenoviral vectors with low seroprevalence thus receives much research attention. here, it is shown that a member from human adenovirus subgroup d, i.e. ad49, does not cross-react with ad5 neutralizing activity, making it ... | 2006 | 16963747 |