| mechanism of allosteric transition of bacterial l-lactate dehydrogenase. | the allosteric behaviour of l-lactate dehydrogenase (l-lactate:nad+oxidoreductase, ec 1.1.1.27, ldh) from bifidobacterium longum am101-2 was studied by means of the subunit hybridization technique as well as x-ray crystallography. homotropic allosteric response of the ldh activity was found against the concentration of its substrate, pyruvate. heterotropic allosteric activation of the enzyme was induced by the addition of fructose 1,6-bisphosphate (fbp). two mutant enzymes, which had either alte ... | 1992 | 1290930 |
| characterization and molecular cloning of bifidobacterium longum cryptic plasmid pmb1. | the small cryptic plasmid pmb1 (1.9 kb), previously isolated from bifidobacterium longum, has been characterized by physical mapping. two cloning vectors, pmr3 and pdg7, carrying chloramphenicol and ampicillin resistances derived from pjh101, have been electroporated in escherichia coli. | 1990 | 1366860 |
| impact of bifidobacterium longum on human fecal microflora. | the effects of bifidobacterium longum feedings for five weeks on the fecal microflora, water contents, ph values, ammonia concentration, and beta-glucuronidase activity were investigated in five healthy human volunteers. although numbers of major bacterial groups of the fecal microflora were not changed by the bifidobacteria feedings, a remarkably decreasing number of lecithinase-negative clostridia was observed. the percentage of lecithinase-negative clostridia and bacteroides to the total bact ... | 1992 | 1406371 |
| transient colonization of the gut of newborn infants by orally administered bifidobacteria and lactobacilli. | we investigated if orally administered bifidobacteria and/or lactobacilli could be cultured from faeces of infants after antibiotic treatment, when these bacterial species are usually absent. lyophilized bifidobacterium longum, strain bb-536, b. breve, strain bb-576, or lactobacillus acidophilus, strain lac-343, were used. doses of 3 x 10(9) cells of one strain, or a mixture of all three strains 3 x 10(9) cells each were fed three times daily at mealtimes to 11 infants aged 0-8 weeks. treatment ... | 1992 | 1421883 |
| cloning and sequence analysis of the gene encoding l-lactate dehydrogenase from lactococcus lactis: evolutionary relationships between 21 different ldh enzymes. | lactate dehydrogenase (ldh; ec1.1.1.27) is a key enzyme in the fermentation of milk by lactic acid bacteria used in the dairy industry. an 800-bp dna fragment containing part of the gene (ldh) encoding ldh was amplified from lactococcus lactis in a polymerase chain reaction using primers designed from the partial amino acid sequence of a lactococcal ldh. this fragment was radioactively labelled and used to probe a phage lambda library of lc. lactis genomic dna. fragments containing ldh were subc ... | 1992 | 1452029 |
| relationship between oxygen sensitivity and oxygen metabolism of bifidobacterium species. | bifidobacteria, which are obligate anaerobes, were studied to determine the relationship between their sensitivity to oxygen and oxygen metabolism. among the four species tested, bifidobacterium infantis, bifidobacterium breve, and bifidobacterium longum differed from bifidobacterium adolescentis in sensitivity to oxygen. the former three species showed marked growth under conditions of partial aeration, whereas the growth of b. adolescentis was suppressed by low concentrations of oxygen. bifido ... | 1992 | 1474198 |
| comparison of carbohydrate substrate preferences in eight species of bifidobacteria. | eight species of bifidobacteria were tested for their abilities to grow on a range of monosaccharides (glucose, arabinose, xylose, galactose and mannose). in contrast to the other sugars, glucose and galactose were utilized by all species and, in general, specific growth rates were highest on these sugars. different substrate preferences were observed between species when the bacteria were grown in the presence of all five monosaccharides. for example, glucose and xylose were coutilized by bifid ... | 1991 | 1778437 |
| quantitative fluorometric assay for rapid enzymatic characterization of bifidobacterium longum and related bifidobacteria. | the quantitative, semi-automated assay described here is an alternative characterization method allowing for highly sensitive and specific detection of bifidobacterial enzymes. twenty strains of bifidobacterium longum, including the type strain atcc 15707, and type strains of 15 other bifidobacterium species were enzymatically characterized using 20 4-methylumbelliferyl conjugated substrates. enzyme activities were determined by directly measuring the intensity of fluorescence derived from 4-met ... | 1991 | 1808458 |
| [antibacterial effect in vitro of bifidobacterium longum on gram-negative bacilli of the fecal flora]. | | 1991 | 1835069 |
| characterization of hu-like protein from bifidobacterium longum. | a hu-like protein (hbl) of bifidobacterium longum was purified and characterized. hbl is heat-stable and acid-resistant, and has a molecular weight of about 9.1 kda as estimated by its mobility on electrophoresis. hbl is intermediate in basicity (pi 9.8) between the hu-1 and hu-2 proteins of escherichia coli, and is dissociated from a calf thymus dna-cellulose column at 300-400 mm nacl. its amino acid composition shows many similarities with that of e coli hu. the nh2-terminal amino acid sequenc ... | 1990 | 2116910 |
| structure of macroamphiphiles from several bifidobacterium strains. | lipoteichoic acid-like substances, macroamphiphiles, were isolated from cell homogenates of bifidobacterium bifidum yit 4007 and yit 4013, bifidobacterium breve yit 4010 and yit 4014, and bifidobacterium longum yit 4021 by phenol extraction followed by nuclease digestion, gel chromatography, ion-exchange chromatography, and hydrophobic interaction chromatography. the macroamphiphile preparations from these five strains contained d-glucose, d-galactose, glycerol, phosphorus, l-alanine, and fatty ... | 1990 | 2298702 |
| growth promotion of bifidobacterium species by whey and casein fractions from human and bovine milk. | an in vitro assay was used to study the growth-promotional activity of human milk (hm), cow's milk (cm), and whey and casein fractions of hm and cm for five strains of bifidobacterium species isolated originally from stools of human infants. whey- and casein-predominant cm-based infant formulas were studied as well. when compared on an equivalent protein basis, the growth promotion activity of hm was greater than that of cm for bifidobacterium bifidum serovar pennsylvanicus and bifidobacterium l ... | 1990 | 2312674 |
| uncoupling of growth and acids production in bifidobacterium ssp. | kinetics of batch cultivation of four species of bifidobacterium in milk were examined in detail. bifidobacteria could grow well in milk inoculated with cultures prepared in a synthetic medium. cessation of growth occurred, however, in ph-controlled batch cultures, although incomplete utilization of lactose was observed. lactate and acetate accumulation caused limitation on growth of bifidobacteria leading to an uncoupling of biomass and product formation. from 70 to 75% of both final lactate an ... | 1990 | 2384614 |
| intestinal microflora in cirrhotic patients and their changes after administration of latamoxef in combination with drug-resistant bifidobacterium longum. | | 1989 | 2519079 |
| [effect of milk fermented with bifidobacterium longum on colonic fermentation]. | | 1989 | 2522631 |
| crystallization of and preliminary crystallographic data for allosteric l-lactate dehydrogenase from bifidobacterium longum. | l-lactate dehydrogenase from bifidobacterium longum am101-2 was overexpressed in escherichia coli and then purified. the enzyme was crystallized from a polyethylene glycol 6000 solution by the hanging drop vapor diffusion method. crystals grown in the presence of nadh (type ii), both nadh and oxamate (type iii), and nadh, oxamate, and fbp (type iv) were analyzed. all three crystal forms belong to the orthorhombic system, space group p2(1)2(1)2. the cell dimensions of the type ii crystals were a ... | 1989 | 2691505 |
| sequence and characteristics of the bifidobacterium longum gene encoding l-lactate dehydrogenase and the primary structure of the enzyme: a new feature of the allosteric site. | the gene ldh, encoding l-lactate dehydrogenase (ldh; ec 1.1.1.27) of bifidobacterium longum am101-2, was cloned in escherichia coli using an oligodeoxyribonucleotide hybridization probe. the amino acid (aa) sequence, deduced from the sequence of the cloned dna, was consistent with the results of protein chemical analysis of b. longum ldh. the transcription start points (tsp) in b. longum were identified by s1 nuclease mapping. a sequence, gtagcaa-(14 bp)-ttataga, which is located a few bp upstre ... | 1989 | 2695396 |
| yoghurt with bifidobacterium longum reduces erythromycin-induced gastrointestinal effects. | | 1987 | 2885529 |
| comparison of populations of human faecal bacteria before and after in vitro incubation with plant cell wall substrates. | human faecal slurries were incubated anaerobically with larchwood xylan, oat spelt xylan, wheat bran, apple cell walls or sugar beet pulp as sole carbon sources. the populations which developed during incubation were different from the inoculum, the most marked changes being an increase in the number of bacteroides species and a decrease in the number of fusobacterium species for all carbon sources tested. with a water-soluble preparation of larchwood xylan the population was dominated by specie ... | 1987 | 3036754 |
| immune responses to mono-associated bifidobacterium longum in mice. | | 1985 | 3161090 |
| menaquinone production and utilization in germ-free rats after inoculation with specific organisms. | although menaquinones are biologically active forms of vitamin k, factors that influence their production by bacteria or their absorption from the gut are not well understood. germ-free rats were inoculated with four different strains of organisms and fecal and tissue menaquinone concentrations were determined. no menaquinones were detected in the tissues or feces of rats colonized with bifidobacterium longum or clostridium ramosum, two organisms that have not been reported to produce menaquinon ... | 1987 | 3298580 |
| deconjugation of bile acids by human intestinal bacteria implanted in germ-free rats. | fecal bile acids in germ-free rats were analyzed after inoculation with bacteroides vulgatus, bifidobacterium longum, escherichia coli or clostridium ramosum. b. vulgatus preferentially deconjugated tauro-beta-muricholic acid and b. longum taurocholic acid. c. ramosum deconjugated both bile acids, but e. coli deconjugated neither. 7 alpha-dehydroxylation of bile acids was negligible even after 18 days of inoculation, but a small amount of 7-oxo-bile acids, less than 5%, was formed. fecal excreti ... | 1987 | 3312906 |
| preparation of the bifidus milk powder. | malt extract and skim milk were used as the culture medium for bifidobacteria. bifidobacterium longum e194b (atcc 15707) selected from among four strains grew well in a nitrogen atmosphere regardless of the initial dissolved oxygen level of the culture medium. the optimum temperature range for growth was 34 to 37 degrees c. the viable cell numbers of bifidobacterium in freeze-dried powder from neutralized bifidus milk were 20 times those found in powder from nonneutralized milk after storage for ... | 1988 | 3410989 |
| related structures in the plasmid profiles of bifidobacterium longum. | the plasmid profiles of 123 strains of b. longum were examined with the southern blot-hybridization technique to reveal the existence of related structures. b. longum is the apparently unique species among those commonly found in man, which harbours extrachromosomal elements. seven different structures were found and their frequency and distribution given. twelve strains were used a source of probes in the hybridization experiments. | 1986 | 3773784 |
| immunological responses to monoassociated bifidobacterium longum and their relation to prevention of bacterial invasion. | after monoassociation of bifidobacterium longum with germ-free balb/c mice (nu/+, heterozygous to nu gene), b. longum was recovered (10(2)-10(4) viable units per organ) from the mesenteric lymph nodes, liver and kidneys for 2 weeks post intragastric inoculation, but not after 4 weeks and later. cessation of bacterial translocation was not observed in monoassociated nude (nu/nu) mice. anti-b. longum iga antibody was detected by elisa using b. longum cell wall antigen in bile from week 1 and in il ... | 1985 | 4043995 |
| barrier effect of bifidobacterium longum on a pathogenic escherichia coli strain by gut colonization in the germ-free rat. | the predominance of bifidobacteria in normal breast-fed babies is well established. even under unfavourable hygienic conditions of delivery and during the breast-feeding period bifidobacteria develop and colonize the intestinal tract at high concentrations. in the present study we investigated the interaction between bifidobacterium longum and a pathogenic e. coli strain in the germ-free rat. sequential counts of the two bacterial strains allowed their proliferation to be followed. electron micr ... | 1984 | 6372273 |
| [use of the gnotobiotic rat for determination of the in vivo activity of bifidobacterium beta-galactosidase]. | under the conditions of in vitro cultivation, the height of the beta-galactosidase activity of bifidobacterium spec. is essentially influenced by the composition of the culture medium. the use of gnotobiotic (germ-free and monoassociated with bifidobacterium longum) rats permitted to differentiate in the chyme between beta-galactosidase activities of mucosal and microbial origin. in germ-free animals, the chyme in the small intestine and the colon contains nearly 10-20% of the activity measured ... | 1984 | 6433203 |
| inhibitory effect of some intestinal bacteria on liver tumorigenesis in gnotobiotic c3h/he male mice. | liver tumorigenesis in gnotobiotic c3h/he male mice was markedly promoted by association with a bacterial combination of escherichia coli, streptococcus faecalis, and clostridium paraputrificum. this study demonstrated that this promoting effect was suppressed by addition of certain intestinal bacteria, such as bifidobacterium longum, lactobacillus acidophilus, and eubacterium rectale. | 1980 | 6780184 |
| plasmids and phages in bifidobacterium longum. | fourteen strains of bifidobacterium longum were tested for phage production with uv and mitomycin c as inducing agents. only four strains released phage-like particles; of these four strains, two harbour plasmids, while two are apparently plasmid free. the induced phages have heads of dimensions ranging from 49 to 56 nm and tails from 76 to 268 nm long. no correlation is evident between any of the large variety of plasmids of b. longum and induced phages. | 1983 | 6865796 |
| purification and certain properties of a bacteriocin from streptococcus mutans. | an inhibition factor from streptococcus mutans strain c3603 (serotype c) was purified and isolated, and its properties indicated that it was a bacteriocin. bacteriocin c3603 is a basic protein with a pi value of 10 and a molecular weight of 4,800. the activity of this bacteriocin was not affected by ph over a range of 1.0 to 12.0 or by storage at 100 degrees c for 10 min at ph 2.0 to 7.0 or storage at 121 degrees c for 15 min at ph 4.0. pronase; papain, phospholipase c, trypsin, and alpha-amylas ... | 1982 | 7068219 |
| influence of breast-feeding on the bifid flora of the newborn intestine. | because of the predominance of bifidobacterium bifidum in the intestine of the breast-fed infant, growth promoting factors were sought in human milk. in vitro, studies showed the presence of specific growth factors for b. bifidum in human milk. other milks, including cow's milk, sheep's milk, pig's milk, and infant formulas did not promote the growth of this species, but did show activity on bifidobacterium infantis and bifidobacterium longum. | 1980 | 7192050 |
| effect of oral administration of lysozyme or digested bacterial cell walls on immunostimulation in guinea pigs. | the effect of oral administration of bacteriolytic enzymes and enzymatically digested bacterial cell walls on immunostimulation in guinea pigs was studied. guinea pigs were given lysozyme or pronase or both orally for a period of 8 days, and on day 7 they were primed with hepatitis b surface antigen. circulating antibody titers to the antigen in the enzyme-treated group were significantly higher (four to six times, p less than 0.05) than those in nontreated control groups on day 16 after immuniz ... | 1981 | 7216461 |
| assessment of bifidobacteria as indicators of human fecal pollution. | the distribution of bifidobacteria in the environment has been examined by using yn-6 medium. although feces of humans, chickens, cows, dogs, pigs, horses, cats, sheep, beavers, goats, and turkeys were examined, bifidobacteria were isolated only from the feces of humans and swine. the frequency and distribution of component species of human fecal isolates were as in isolates from raw sewage. bifidobacterium longum and b. adolescentis were most often isolated and in the highest densities. the lev ... | 1981 | 7294781 |
| streptococcus faecium-derived antibacterial substance antagonistic to bifidobacteria. | an antagonistic strain of streptococcus faecium was isolated from human feces, and it displayed a marked inhibition of bifidobacteria on agar plates. in liquid culture this isolate produced an antibacterial substance that can be partially purified by ammonium sulfate precipitation followed by gel filtration and ion-exchange chromatography. its activity was assayed by the inhibition of growth of bifidobacterium longum. the substance was sensitive to digestion by proteolytic enzymes and alpha-amyl ... | 1980 | 7416752 |
| structural studies on a cell wall polysaccharide from bifidobacterium longum yit4028. | the major fraction of rhamnogalactan was isolated from the cell wall of bifidobacterium longum yit4028 by treatment with n-acetylmuramidase. this polysaccharide was composed of rhamnose and galactose in a molar ratio of about 2:3. the 13c nmr spectrum indicated that it contained a pentasaccharides repeating unit. this observation, and the results of smith degradation, partial acid hydrolysis and methylation analysis led to the conclusion that its structure is [formula: see text] | 1995 | 7585708 |
| t and r states in the crystals of bacterial l-lactate dehydrogenase reveal the mechanism for allosteric control. | the crystal structure of l-lactate dehydrogenase from bifidobacterium longum, determined to 2.5 a resolution, contains a regular 1:1 complex of t- and r-state tetramers. a comparison of these two structures within the same crystal lattice and kinetical characterization of the t-r transition in solution provide an explanation for the molecular mechanism of allosteric activation. substrate affinity is controlled by helix sliding between subunits which is triggered by the binding of the activator, ... | 1994 | 7656036 |
| viability and enzymatic activity of bifidobacteria in milk. | bifidobacterium breve ncfb 2258, bifidobacterium bifidum ncfb 2715, bifidobacterium longum atcc 15707, bifidobacterium angulatum atcc 27535, and lactobacillus acidophilus n2 were evaluated for viability and beta-galactosidase and alpha-galactosidase activities under conditions of refrigerated and frozen storage in reconstituted ndm. beta- and alpha-galactosidase activities were variable. bifidobacterium angulatum 27535 exhibited much greater enzymatic activities than those of the other strains. ... | 1995 | 7745146 |
| selection of species-specific dna probes which detect strain restriction polymorphism in four bifidobacterium species. | randomly cloned fragments (in a size range 1 to 2.5 kb) of dna from bifidobacterium longum atcc 15707, b. adolescentis cip 64.59t, b. bifidum cip 64.65 and b. animalis atcc 25527 were used as hybridization probes to characterize strains of these species and distinguish them from closely related bifidobacterium species. the fragments were screened for hybridization with native dna from 41 different bifidobacterium strains. for each species, a fragment hybridizing specifically with dna from strain ... | 1995 | 7754230 |
| effect of chelatants on gellan gel rheological properties and setting temperature for immobilization of living bifidobacteria. | the effect of various concentrations of sequestrants (sodium citrate, sodium metaphosphate, and edta) was studied on gellan gel (1.5-2.5% (w/v)) setting temperature and rheological properties. addition of edta between 0 and 0.8% (w/v) led to a progressive decrease of setting temperature. citrate and metaphosphate decreased this parameter when added up to 0.4 or 0.6%, depending on gellan gum concentration, eventually resulting in the absence of gel formation at room temperature for the 1.5% gella ... | 1993 | 7763698 |
| effect of bifidobacteria on nitrites and nitrosamines. | the effects of six different bifidobacteria strains were studied on two procarcinogens: nitrite and nitrosamines. growth of bifidobacteria was not affected by nitrite concentrations below 50 mumol l-1. at nitrite concentrations greater than 2000 mumol l-1, total growth inhibition was observed. nitrite elimination by a non-enzymic mechanism was noted for six strains of bifidobacteria. acids produced by the bacteria seem to be involved in nitrite elimination. nitrosamines tested had no effect on g ... | 1995 | 7766234 |
| bifidobacteria and probiotic effects: action of bifidobacterium species on conjugated bile salts. | the effect of six different conjugated bile salts (two trihydroxyconjugated bile salts: tauro and glycocholic acids; and four dihydroxyconjugated bile salts: tauro- and glycochenodeoxycholic, tauro- and glycodeoxycholic acids) on eight bifidobacteria strains were studied. a strong growth-inhibitory effect was observed (80% at 0.95 mm) for each bile salt and strain. this phenomenon was explained by the production of deconjugated bile salt during bifidobacteria growth. the deconjugation phenomenon ... | 1995 | 7767225 |
| characterization of fructose 6 phosphate phosphoketolases purified from bifidobacterium species. | fructose 6 phosphate phosphoketolases (f6ppks) were purified from bifidobacterium longum bb536, b. dentium atcc 27534, b. globosum atcc 25864, and bifidobacterium animalis atcc 25527. concerning ions (cu++, zn++, ca++, mg++, fe++, co++, mn++) and common enzyme inhibitors (fructose, ammonium sulfate, iodoacetate, and parachloromercuribenzoic acid), no difference appeared between the enzymes. cu++, parachloromercuribenzoic acid (pcmb), and mercuric acetate induced high enzymatic inhibition. the st ... | 1995 | 7767228 |
| inhibitory effect of bifidobacterium longum cultures on the azoxymethane-induced aberrant crypt foci formation and fecal bacterial beta-glucuronidase. | epidemiologic and experimental studies suggest that consumption of fermented milk products and lactic bacterial cultures that are used to ferment the dairy products, decrease the incidence of certain types of cancer. the present study was designed to determine the effect of lyophilized cultures of bifidobacterium longum (b. longum), a lactic bacteria, on the azoxymethane (aom)-induced preneoplastic lesions such as aberrant crypt foci (acf) formation in the colon and on fecal bacterial beta-glucu ... | 1994 | 7800683 |
| absence of cholic acid 7 alpha-dehydroxylase activity in the strains of lactobacillus and bifidobacterium. | to investigate the presence of 7 alpha-dehydroxylase activity on bile acids in the bacterial strains of fermented milk products, 46 strains of lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus acidophilus, lactobacillus gasseri, bifidobacterium adolescentis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, lactococcus lactis spp. lactis, and streptococcus salivarius spp. thermophilus were tested for their ability ... | 1994 | 7814703 |
| transformation of bifidobacterium longum with prm2, a constructed escherichia coli-b. longum shuttle vector. | an escherichia coli-bifidobacterium longum shuttle vector, designated prm2, was constructed by cloning a b. longum plasmid and an enterococcal spectinomycin resistance gene into a commercial e. coli vector. the plasmid was successfully introduced into b. longum cells by electroporation and into e. coli cells by both electroporation and chemical transformation. | 1994 | 7846144 |
| origin of d-alanine present in urine of mutant mice lacking d-amino-acid oxidase activity. | urine of mutant ddy/dao- mice lacking d-amino-acid oxidase contained 13 times more d-alanine than that of normal ddy/dao+ mice. because d-alanine is a component of bacterial cell walls, the possibility that the urinary d-alanine came from intestinal bacteria was examined. in ddy/dao- mice that were made germ free at birth and reared in a germ-free environment, the quantity of urinary d-alanine was found to be at a low level comparable to that of the normal mice. when these germ-free mice were ma ... | 1993 | 7901999 |
| microbiological evaluation of jejunal aspirates and faecal samples after oral administration of bifidobacteria and lactic acid bacteria. | a double-blind placebo controlled investigation was carried out to study the effect of peroral colonization. human volunteers were given mixtures of bifidobacteria and lactic acid bacteria. measurements were made over a 1 week treatment period and for another week after the end of the treatment. two different bacteriological preparations were used, one consisted of enterococcus faecium and bifidobacterium longum (a total of 6.4 x 10(8) cfu d-1); the other consisted of lactobacillus acidophilus, ... | 1994 | 8005834 |
| a regular 1:1 complex of two allosteric states in the single crystal of l-lactate dehydrogenase from bifidobacterium longum. | single crystals of the regular 1:1 complex of t and r-state tetramers of l-lactate dehydrogenase from bifidobacterium longum have been grown from polyethylene glycol 6000 solution in the presence of nadh, fructose 1,6-bisphosphate and oxamate. the crystals belong to space group f222 with unit cell dimensions of a = 148.4 a, b = 295.9 a and c = 71.0 a. the crystals are suitable for x-ray analysis and diffract to beyond 2.5 a spacing. a crystallographic study showed that the asymmetric unit contai ... | 1994 | 8114106 |
| allosteric activation in bacillus stearothermophilus lactate dehydrogenase investigated by an x-ray crystallographic analysis of a mutant designed to prevent tetramerization of the enzyme. | the crystal structure of a mutant bacillus stearothermophilus lactate dehydrogenase, into which an additional loop has been engineered in order to prevent tetramerization of the enzyme, has been solved and refined at 2.4 a. the minimal repeat unit in the crystal is a dimer and the tetramer cannot be generated by any of the crystallographic symmetry operations in p2(1). the loop protrudes out into the solvent, stabilized by a good hydrogen bonding arrangement, and clearly sterically hinders tetra ... | 1994 | 8176749 |
| delta 22-beta-muricholic acid in monoassociated rats and conventional rats. | bile acids were analyzed in the bile, small and large intestines, and feces of germ-free rats after a single inoculation with one of six intestinal bacteria that had been originally isolated from human feces. bacteroides vulgatus and bifidobacterium longum preferentially deconjugated tauro-beta-muricholic acid and taurocholic acid, respectively. clostridium ramosum, peptostreptococcus productus and lactobacillus gasseri deconjugated both bile acids, but escherichia coli did not deconjugate eithe ... | 1994 | 8177022 |
| [isolation and identification of an oxygen-tolerant strain of bifidobacterium from human feces]. | this paper reports 136 strains suspected to be species of the genes bifidobacterium isolated from adult human faeces. through detection of the ability to tolerate molecular oxygen in cultural environments, we obtained an oxygen-resistant strain tq21-2-2 according to the bergy's manual. it was identified as bifidobacterium longum. a limited number of drinking the beverage, the experiments also using this strain in milk as a starter revealed that this strain has been found useful for treating case ... | 1994 | 8209550 |
| augmentation of macrophage phagocytic activity by cell-free extracts of selected lactic acid-producing bacteria. | oral and intraperitoneal administration of lactic acid-producing bacteria can significantly augment the immune response in murine models; however, the immunopotentiating effects in these studies differ significantly. murine macrophagelike cell line j774 was cultured in the presence of cell-free extracts of lactobacillus acidophilus and bifidobacterium longum, and the effect on macrophage function was evaluated by measurement of synthesis of selected enzymes and their ability to take up either ac ... | 1993 | 8227651 |
| does yogurt enriched with bifidobacterium longum affect colonic microbiology and fecal metabolites in health subjects? | diet-induced changes in the colonic microflora seem to play a role in colon carcinogenesis. in this study the effects of a yogurt (500 ml/d for 3 wk) enriched with bifidobacterium longum and 5 g lactulose/l (a) on the fecal bacterial flora and various risk indexes for colon carcinogenesis were tested in 12 healthy volunteers and compared with a conventional yogurt (b). increased excretion of bifidobacteria (p < 0.017) was found after consumption of both yogurts compared with the prestudy periods ... | 1994 | 8310997 |
| phenotypic and genomic analyses of human strains belonging or related to bifidobacterium longum, bifidobacterium infantis, and bifidobacterium breve. | a numerical analysis based on phenotypic characteristics (89 enzymatic tests and 49 carbohydrate acidification tests), in which experimental strips from biomerieux-api, la balme les grottes, france, were used, was performed to characterize 82 new isolates belonging or related to bifidobacterium longum, bifidobacterium infantis, and bifidobacterium breve. a total of 72 strains were isolated from child or adult feces, and the other strains were obtained from human vaginas and bronchi. in this stud ... | 1993 | 8347514 |
| inhibitory effect of bifidobacterium longum on colon, mammary, and liver carcinogenesis induced by 2-amino-3-methylimidazo[4,5-f]quinoline, a food mutagen. | the inhibitory effect of lyophilized cultures of bifidobacterium longum on 2-amino-3-methylimidazo[4,5-f]quinoline (iq)-induced carcinogenesis was investigated in male and female f344 rats. beginning at 5 weeks of age, male and female rats were divided into various experimental groups and fed one of the high-fat, semipurified diets containing 0 and 0.5% lyophilized cultures of b. longum with or without 125 ppm iq in the diet. all animals were continued on this regimen until the termination of th ... | 1993 | 8358717 |
| analysis of intestinal flora of a patient with congenital absence of the portal vein. | a 14-year-old female patient, admitted for a closer examination of liver tumour (hepatocellular adenoma), was diagnosed as having a congenital absence of the portal vein. the blood ammonia level (approximately 120 micrograms dl-1) in the superior mesenteric vein was markedly low compared to the normal value of 300-350 micrograms dl-1 in the portal vein. the decreased ammonia concentration and urease activity of the patient's faeces were demonstrated. the dominant intestinal flora in the faeces o ... | 1993 | 8364525 |
| molecular basis of allosteric activation of bacterial l-lactate dehydrogenase. | the three-dimensional structure of allosteric l-lactate dehydrogenase from bifidobacterium longum, the first example of a t-state structure of l-lactate dehydrogenase, has been determined to 2.0 a. a comparative study of this structure with the previously reported r-state structure from bacillus stearothermophilus has revealed the allosteric activation mechanism of the bacterial l-lactate dehydrogenase. the fructose 1,6-bisphosphate-induced conformational change at the effector site and the subs ... | 1993 | 8450537 |
| growth characteristics of bifidobacteria in ultrafiltered milk. | five replicates of raw skim milk were ultrafiltered at 54 degrees c to total protein concentration ratios of 2:1, 3:1, 4:1, and 5:1. bifidobacterium bifidum and bifidobacterium longum were inoculated at 5% into skim milk that was not ultrafiltered (1:1) and into ultrafiltered skim milks followed by incubation at 37 degrees c. the mean maximum bacterial count (colony-forming units per milliliter) range and protein concentration ratio were from 6.25 x 10(8) to 1.69 x 10(9), skim milk; 4.42 x 10(8) ... | 1993 | 8486847 |
| therapeutic use of an antibiotic-resistant bifidobacterium preparation in men exposed to high-dose gamma-irradiation. | five men received high-dose, uneven, whole-body gamma-irradiation by accidental exposure to an unshielded 137cs source. analysis of the faeces 9-12 days post-irradiation showed low numbers of anaerobes and high counts of enterobacteria and staphylococci in four of the patients and total viable counts of < 10(3)/g in one. all five were treated with systemic ampicillin and gentamicin and oral nystatin commencing 4-7 days after irradiation. three of the patients were also treated orally with a susp ... | 1996 | 8544215 |
| isolation and characterization of exocellular polysaccharides produced by bifidobacterium longum. | when grown anaerobically at ph values above 5.0, on ultrafiltered complex media containing excess lactose, bifidobacterium longum formed up to 140 mg l-1 (glucose equiv.) exopolysaccharides. the highest yield was obtained when the cells were cultivated in a peptone/yeast extract medium with ph controlled by additions of nh4oh. whatever the conditions under study, exopolysaccharides represented about 30% of the polysaccharides produced by b. longum after 48 h of culture. crude pronase-treated exo ... | 1995 | 8590666 |
| biotherapeutic agents. a neglected modality for the treatment and prevention of selected intestinal and vaginal infections. | to evaluate the potential of biotherapeutic agents (microorganisms with therapeutic properties) for the prevention and/or treatment of selected intestinal and vaginal infections. | 1996 | 8596226 |
| characterization of the plasmid pmb1 from bifidobacterium longum and its use for shuttle vector construction. | the nucleotide sequence of the 1847-bp bifidobacterium longum b2577 cryptic plasmid pmb1 was determined. the plasmid had a g+c content of 62.0%, and contained two open reading frames, orf1 and orf2, likely arranged in an operon. the proteins encoded by orf1 and orf2 show the highest degree of similarity with similarly arranged peptide sequences translated from corynebacterium glutamicum pxz10142 and mycobacterium fortuitum pal5000 plasmids. recombinant plasmids containing the pmb1 replicon were ... | 1996 | 8761732 |
| improvement of lactose digestion in humans by ingestion of unfermented milk containing bifidobacterium longum. | fifteen lactose malabsorbers were studied to evaluate the effects of consumption of milk containing different strains of bifidobacterium longum on lactose digestion. influences of different growth substrates, bile sensitivity, and lactose transport on lactose digestion by bifidobacteria were also investigated. lactose malabsorption was determined by measuring breath hydrogen excretion of subjects fed four different test milks (three of which contained 5 x 10(8) cfu/ml of b. longum) on 4 differen ... | 1996 | 8792277 |
| allosteric activation of l-lactate dehydrogenase analyzed by hybrid enzymes with effector-sensitive and -insensitive subunits. | subunit-hybrid enzymes of mutant tetrameric l-lactate dehydrogenases from bifidobacterium longum were studied in an examination of the mechanism of allosteric activation by fructose 1,6-bisphosphate. we earlier developed an in vivo method for subunit hybridization in escherichia coli and the hybrids formed were a mixture with different subunit compositions. the b. longum hybrids were separated by anion-exchange chromatography with a mutational tag. hybrids formed between fructose 1,6-bisphosphat ... | 1996 | 8810336 |
| growth characteristics of bifidobacteria in infant formulas. | four species of bifidobacteria, bifidobacterium bifidum (atcc 15696), bifidobacterium breve (atcc 15700), bifidobacterium infantis (atcc 15697), and bifidobacterium longum (atcc 15708), were grown anaerobically at 37 degrees c in lactobacilli mrs broth with 0.5% cysteine-hc1 and inoculated at 2.5% into three types of infant formula (based on soy, or milk, or casein hydrolysate) and in nonfat milk followed by incubation at 37 degrees c for 24 h. in most cases, the logarithmic phase of growth for ... | 1996 | 8872711 |
| effect of bifidogenic factors on growth characteristics of bifidobacteria in infant formulas. | a bifidogenic factor, lactulose or fructooligosaccharides, was added (0.5%) into infant formula based on soy or infant formula with hydrolyzed casein. the infant formulas were then inoculated (2.5%) with bifidobacterium bifidum (atcc 15696), bifidobacterium breve (atcc 15700), bifidobacterium infantis (atcc 15697), or bifidobacterium longum (atcc 15708) or their mixture (mixed culture) and incubated at 37 degrees c for 24 h. lactulose did not influence maximal counts or generation times in eithe ... | 1996 | 8872712 |
| lactobacillus- and bifidobacterium-mediated antigenotoxicity in the colon of rats. | lactic acid bacteria (lab) are proposed to have several beneficial effects, including the inactivation of carcinogens. we have studied the potential of lactobacillus acidophilus (from a commercially available yogurt), lactobacillus gasseri (p79), lactobacillus confusus (dsm20196), streptococcus thermophilus (ncim 50083), bifidobacterium breve and bifidobacterium longum (from human infant stool) to prevent the induction of dna damage by n-methyl-n'-nitro-n-nitrosoguanidine (mnng, 7.5 mg/kg body w ... | 1996 | 8910918 |
| pcr detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples. | pcr procedures based on 16s rrna gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mouse, cat, dog, monkey, and rabbit) feces. fusobacterium prausnitzii, peptostreptococcus productus, and clostridium clostridiiforme had high pcr titers (the maximum dilutions for positive pcr results ranged from 10(-3) to 10(-8)) in all of the human an ... | 1996 | 8919784 |
| proteinaceous factor(s) in culture supernatant fluids of bifidobacteria which prevents the binding of enterotoxigenic escherichia coli to gangliotetraosylceramide. | we have examined the competitive binding of several species of bifidobacterium and escherichia coli pb176, an enterotoxigenic e. coli (etec) strain, to gangliotetraosylceramide (asialo gm1 or ga1), a common bacterium-binding structure, and identified a factor(s) in the bifidobacterium culture supernatant fluid that inhibits the binding of e. coli pb176 to ga1. the etec strain we used expresses colonization factor antigen (cfa) ii, which consists of coli surface-associated antigens cs1 and cs3. c ... | 1997 | 9023929 |
| bifidobacterium longum and lactulose suppress azoxymethane-induced colonic aberrant crypt foci in rats. | bifidobacterium longum has been shown to afford protection against colon tumorigenesis. lactulose, a keto analog of lactose, serves as a substrate for preferential growth of bifidobacterium. it is not known whether feeding lactulose along with b. longum will have any advantage over feeding of b. longum alone. to test this combination effect, 61 male fisher 344 weanling rats were divided into four groups of 15 rats each (16 in the control group) and assigned to one of the following four diets for ... | 1997 | 9067551 |
| bifidobacterium longum, a lactic acid-producing intestinal bacterium inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis. | the human colon can be described as a complex microbial ecosystem, comprising several hundred bacterial species. some of these enteric bacteria are beneficial to the host and have been shown to exert antimutagenic and anticarcinogenic properties. we have investigated the colon tumor inhibitory activity of bifidobacterium longum, a lactic acid-producing enterobacterium. the modifying effects of this lactic culture on colonic mucosal and/or tumor cell proliferation, odc activity and ras-p21 oncopr ... | 1997 | 9111222 |
| investigations of bifidobacterial ecology and oligosaccharide metabolism in a three-stage compound continuous culture system. | several different types of in vitro fermentation systems are currently employed to investigate pro- and prebiotic activities in the human large intestinal microbiota, ranging from simple batch cultures, with or without stirring and ph control, to more complex models involving ph controlled single and multiple-component continuous culture systems. | 1997 | 9145444 |
| influence of carcinogen binding by lactic acid-producing bacteria on tissue distribution and in vivo mutagenicity of dietary carcinogens. | the aims of this investigation were to determine whether viable cultures of lactic acid-producing organisms (lab) can bind dietary carcinogens and to assess the consequences of binding for the absorption from the gut, distribution in the body and in vivo genotoxicity of ingested carcinogens. the carcinogens used in this study were ones known to be present in the human diet, namely benzo[a]pyrene (b(a)p, aflatoxin b1 (afb1) and the cooked food carcinogens 2-amino-3-methyl-3h-imidazo[4,5-f]quinoli ... | 1997 | 9225011 |
| digoxigenin-labeled deoxyribonucleic acid probes for the enumeration of bifidobacteria in fecal samples. | the numbers of bifidobacteria in fecal samples were specifically determined by colony hybridization with the mixture of digoxigenin-labeled dna probes that were prepared from whole chromosomal dna of bifidobacterium longum 6001 and bifidobacterium adolescentis 6003. these dna probes strongly hybridized with dna of b. longum, b. adolescentis, bifidobacterium breve, bifidobacterium suis, bifidobacterium infantis, bifidobacterium bifidum, bifidobacterium angulatum, and bifidobacterium animalis. det ... | 1997 | 9241587 |
| isolation and characterization of two plasmids from bifidobacterium longum. | in order to develop a cloning vector system which can be used in bifidobacterium sp., we screened about 100 bifidobacteria from the faeces of adults and children. among them, only one strain, identified as b. longum kj, was shown to contain extrachromosomal dnas. bifidobacterium longum kj showed multiple plasmid dna bands which were resolved to be multimers of two plasmids designated pkj36 and pkj50. these plasmids were cloned into the escherichia coli vector puc19 as pms36 and pms50, respective ... | 1997 | 9248072 |
| construction of escherichia coli-bifidobacterium longum shuttle vector transforming b. longum 105-a and 108-a. | a shuttle vector, pbles100, was constructed by cloning a bifidobacterium longum plasmid and a gene encoding spectinomycin adenyltransferase aad(9) from enterococcus faecalis into the escherichia coli vector pbr322. stable transformants with this plasmid were obtained with an efficiency of 2.2 x 10(4) transformants/microgram dna or 6.9 x 10(-5) transformants/cell/microgram dna under the optimal conditions of 10.0 kv/cm, 200 omega, and 25 microf, using b. longum 105-a harvested at late log phase o ... | 1997 | 9255988 |
| fecal numbers of bifidobacteria are higher in pigs fed bifidobacterium longum with a high amylose cornstarch than with a low amylose cornstarch. | twelve young male pigs consumed a purified diet containing wheat bran as fiber source. starch provided 50% of total daily energy either as a low amylose cornstarch or as a high amylose (amylomaize) starch. the pigs were given a supplement of a freeze-dried probiotic organism (bifidobacterium longum cscc 1941). a block crossover design was used so that at any one time two groups of three pigs consumed either the high or low amylose cornstarch without probiotic and a further two groups of three pi ... | 1997 | 9278566 |
| inhibition of bacterial translocation from the gastrointestinal tract of mice by oral administration of a culture condensate of bifidobacterium longum. | bacterial translocation occurs when viable bacteria pass through the mucosa of the gastrointestinal tract to the mesenteric lymph nodes (mln) and other organs. the ability of a diet containing 1% culture condensate of bifidobacterium longum (mb) to inhibit bacterial translocation from the gastrointestinal tract was tested using antibiotic-decontaminated specific-pathogen-free (spf) mice and germ-free mice, both of which were monoassociated with escherichia coli c25. feeding of mb diet decreased ... | 1997 | 9300362 |
| homotropic activation via the subunit interaction and allosteric symmetry revealed on analysis of hybrid enzymes of l-lactate dehydrogenase. | l-lactate dehydrogenase from bifidobacterium longum shows homotropic activation by pyruvate as well as heterotropic activation by fructose 1,6-bisphosphate. hybrid enzymes were produced from the wild-type subunit and a mutant subunit, whose substrate specificity was altered to that of malate dehydrogenase, and separated to analyze the substrate-induced homotropic activation mechanism. oxamate, a competitive inhibitor of l-lactate dehydrogenase, was used to mimic the substrate-induced activation ... | 1998 | 9446610 |
| effect of bifidobacterium longum and inulin on gut bacterial metabolism and carcinogen-induced aberrant crypt foci in rats. | the effect of bifidobacterium longum (4 x 10(8) viable cells/g diet) and a derivative of inulin ('raftiline hp'; 5% w/w in diet) on colonic aberrant crypt foci (acf) induced by the colon carcinogen azoxymethane (aom) has been studied. the concentration of ammonia, a putative tumour promoter produced by bacterial degradation of protein and urea, and the activities of certain bacterial enzymes thought to play a role in colon carcinogenesis, beta-glucuronidase and beta-glucosidase were also assayed ... | 1998 | 9498277 |
| effects of orally ingested bifidobacterium longum on the mucosal iga response of mice to dietary antigens. | to study the effects of lactic acid bacteria on the mucosal defence against dietary protein antigens, we compared the mucosal iga responses to beta-lactoglobulin (beta-lg) of two groups of mice fed a whey protein diet with and without a culture condensate of bifidobacterium longum. both total iga and anti-beta-lg iga levels in tissue extracts of the small intestinal wall were significantly higher in mice fed the b. longum diet for 2 weeks than in control ones. peyer's patch (pp) cells from b. lo ... | 1998 | 9501512 |
| growth, viability, and proteolytic activity of bifidobacteria in whole camel milk. | four species of bifidobacteria were evaluated for growth, viability, and proteolytic activity in whole camel milk, and comparison was made with whole cow milk. growth of all species in either whole milk was characterized by the appearance of two logarithmic phases following anaerobic incubation at 37 degrees c for 36 h. the growth rate of bifidobacterium longum 15,707 was higher in camel milk than in bovine milk and was higher for bifidobacterium angulatum 27,535 in bovine milk than in camel mil ... | 1998 | 9532489 |
| improved cloning vectors for bifidobacterium spp. | the recombinant plasmids pdli41, pdga7 and pdco7 were constructed by cloning in pdg7, a vector based on bifidobacterium longum replicon pmb1, the following heterologous genes: pseudomonas fluorescens lipase, bacillus licheniformis alpha-amylase and streptomyces sp. cholesterol oxidase. the hybrid plasmids efficiently transformed bifidobacterium belonging to five different species. a novel escherichia coli-bifidobacterium set of shuttle vectors based on the replicon pmb1 (plf5, pclj15, pspec1) fe ... | 1998 | 9569689 |
| inhibition of initiation and early stage development of aberrant crypt foci and enhanced natural killer activity in male rats administered bovine lactoferrin concomitantly with azoxymethane. | the influence of concomitant administration of bovine lactoferrin (blf) on induction of aberrant crypt foci (acf) by azoxymethane was investigated in male f344 rats. two percent blf and 3% bifidobacterium longum (b. longum), as a positive control, significantly decreased the numbers of acf as well as the total numbers of aberrant crypts reproducibly in three independent studies (2% blf, p < 0.01; 3% b. longum, p < 0.05). most importantly large size foci composed of four or more crypts were alway ... | 1997 | 9570361 |
| supplementation of bifidobacterium longum to a high-fat, low-calcium diet lowers cytolytic activity of fecal water in rats injected with 1,2-dimethylhydrazine dihydrochloride. | the effects of supplementing bifidobacterium longum sbt 2928 and lactobacillus acidophilus sbt 2062 to a high-fat, low-calcium diet on bile acid concentration, fatty acid concentration, cytolytic activity and intestinal alkaline phosphatase (alp) activity of fecal water in rats injected with and without 1,2-dimethylhydrazine dihydrochloride (dmh) were examined. male wistar rats at 8 weeks of age were fed a diet containing 18% coconut oil, 2% corn oil and 0.1% calcium for 15 d. lyophilized cultur ... | 1998 | 9591245 |
| antimutagenicity and the influence of physical factors in binding lactobacillus gasseri and bifidobacterium longum cells to amino acid pyrolysates. | antimutagenic and binding properties of 28 strains of lactobacillus gasseri and 2 strains of bifidobacterium longum on the mutagenicity of amino acid pyrolysates were investigated in vitro using a streptomycin-dependent (sd510) strain of salmonella typhimurium ta 98. four strains of l. acidophilus (sbt0274, sbt1703, sbt10239, and sbt10241) and 1 strain of b. longum (sbt 2928) exhibited the highest percentage of antimutagenicity and binding. these 5 strains were further optimized for other physic ... | 1998 | 9684159 |
| incorporation of cholesterol into the cellular membrane of bifidobacterium longum. | four strains of bifidobacterium longum were isolated from feces of human intestinal sources and were tested along with four commercially available strains of b. longum for bile tolerance, the ability to deconjugate sodium taurocholate, and the ability to remove cholesterol from the culture medium. the cultures varied with respect to each test. there was no relationship between bile tolerance and the ability to remove cholesterol. relative deconjugation of sodium taurocholate also was not related ... | 1998 | 9710749 |
| growth of a human intestinal desulfovibrio desulfuricans in continuous cultures containing defined populations of saccharolytic and amino acid fermenting bacteria. | ecological and physiological effects of the sulphate-reducing bacterium (srb) desulfovibrio desulfuricans on other intestinal organisms were investigated in anaerobic chemostats (dilution rate approximately 0.2 h-1). reproducible defined bacterial communities were used in these experiments, comprising 14 different saccharolytic and amino acid fermenting species: bifidobacterium longum, bif. adolescentis, bif. pseudolongum, bif. infantis, bacteroides thetaiotaomicron, bact. vulgatus, lactobacillu ... | 1998 | 9750310 |
| production of a mixture of antimicrobial organic acids from lactose by co-culture of bifidobacterium longum and propionibacterium freudenreichii. | the antimicrobial activities of standard solutions of three organic acids (lactic, acetic, and propionic acids) were compared using micrococcus luteus, pseudomonas sp. and staphylococcus aureus as test microorganisms. at the same concentrations of the undissociated form, the antimicrobial activities of acetic and propionic acids were higher than that of lactic acid, irrespective of test microorganisms. in a single cultivation of bifidobacterium longum, a mixture of lactic (17 g/l) and acetic (20 ... | 1998 | 9757558 |
| inhibition of azoxymethane initiated colon tumor and aberrant crypt foci development by bovine lactoferrin administration in f344 rats. | the influence of bovine lactoferrin (blf) on colon carcinogenesis was investigated in male f344 rats treated with azoxymethane (aom). in experiment i, 2% and 0.2% blf, and bifidobacterium longum (b. longum) as a positive control at 3% were given in the diet for 4 weeks, along with two s.c. 15 mg/kg injections of aom on days 1 and 8. the numbers of aberrant crypt foci (acf) were decreased by both treatments. similar results were obtained in experiment ii of 13 weeks duration. in experiment iii, a ... | 1998 | 9781370 |
| increased growth of bifidobacterium and eubacterium by germinated barley foodstuff, accompanied by enhanced butyrate production in healthy volunteers. | germinated barley foodstuff (gbf) derived from the aleurone and scutellum fractions of germinated barley mainly consists of low-lignified hemicellulose and glutamine-rich protein. gbf improves the proliferation of intestinal epithelial cells and defecation, through the bacterial production of short chain fatty acids (scfa), especially butyrate. in this study we investigated the mechanism of production of butyrate by microflora in humans and in vitro. daily administration of 9 g gbf for 14 succes ... | 1999 | 9917526 |
| prevention of colon cancer by pre- and probiotics: evidence from laboratory studies. | oligofructose and inulin, selective fermentable chicory fructans, have been shown to stimulate the growth of bifidobacteria which are regarded as beneficial strains in the colon. studies were designed to evaluate inulin (raftiline) and oligofructose (raftilose), for their potential inhibitory properties against aberrant crypt foci (acf) formation in the colon of rats. acf are putative preneoplastic lesions from which adenomas and carcinomas may develop. the results of this study demonstrate that ... | 1998 | 9924288 |
| the antimutagenic properties of a polysaccharide produced by bifidobacterium longum and its cultured milk against some heterocyclic amines. | the antimutagenicity and fermentation pattern of three bifidobacterium longum strains (b. longum, b. longum ps+, and b. longum ps-) in skim milk were studied. the increase in fermentation time significantly increased antimutagenicity with all strains tested against the mutagenicity of both 3-amino-1,4-dimethyl-5h-pyrido-[4,3-b]indole (trp-p-1) and 3-amino-1-methyl-5h-pyrido[4,3-b]indole (trp-p-2) in an ames-like test using streptomycin-dependent strain sd510 of salmonella typhimurium ta98. bifid ... | 1998 | 10029998 |
| note: evaluation of selective media for the enumeration of bifidobacterium sp. in milk. | pure cultures of three species of bifidobacteria (bifidobacterium longum, bif. adolescentis and bif. bifidum), lactobacillus acidophilus and a mixed culture of lact. delbrueckii subsp. bulgaricus and streptococcus salivarius subsp. thermophilus were each enumerated on two differential media and six selective media for the enumeration of bifidobacteria. the appearance of the colonies on the differential media was as expected but when mixed cultures were present, it proved extremely difficult to d ... | 1999 | 10063635 |
| case of sepsis caused by bifidobacterium longum. | we report a case of sepsis caused by bifidobacterium longum in a 19-year-old male who had developed high fever, jaundice, and hepatomegaly after acupuncture therapy with small gold needles. anaerobic, non-spore-forming, gram-positive bacilli were isolated from his blood and finally identified as b. longum. he recovered completely after treatment with ticarcillin and metronidazole. to our knowledge, this is the first report of incidental sepsis caused by b. longum. | 1999 | 10074561 |
| purification and characterization of a novel protein produced by bifidobacterium longum sbt2928 that inhibits the binding of enterotoxigenic escherichia coli pb176 (cfa/ii) to gangliotetraosylceramide. | a novel protein (bif) which shows inhibitory activity on the binding of enterotoxigenic escherichia coli pb176 (etec with colonization factor antigen (cfa) ii, which consists of coli surface-associated antigens cs1 and cs3) to gangliotetraosylceramide (asialo gm1 or ga1) was isolated from the culture supernatant fluid of bifidobacterium longum sbt2928 (bl2928) at its stationary phase. the homogeneity of the final preparation of bif was demonstrated by sds-page, polyacrylamide gel electrofocusing ... | 1999 | 10212407 |
| sequence analysis of plasmid pkj50 from bifidobacterium longum. | the complete nucleotide sequence of a plasmid, pkj50, isolated from an intestinal bacterium, bifidobacterium longum kj, has been determined. the plasmid was analysed and found to be 4960 bp in size with a g+c content of 61.7 mol%. computer analysis of sequence data revealed three major orfs encoding putative proteins of 31.5 (orfi), 24.5 (orfii) and 38.6 kda (orfiii). orfi encodes a protein with a pi of 10.18 and shows relatively high amino acid sequence similarity (more than 60%) with several p ... | 1999 | 10217492 |
| strain dependency of the immunopotentiating activity of lactobacillus delbrueckii subsp. bulgaricus. | to obtain strains of lactobacillus delbrueckii subsp. bulgaricus with high immunopotentiating activity, we screened 90 strains of this bacterial species for the proliferative response of murine spleen and beta-lactoglobulin-primed lymph node cells. in this screening, certain strains showed strong immunopotentiating activity. among them, strain 1023 had the strongest mitogenic activity for murine peyer's patch (pp) cells. furthermore, strain 1023 induced iga antibody production by pp cells as str ... | 1999 | 10227134 |
| possible mechanisms by which pro- and prebiotics influence colon carcinogenesis and tumor growth. | oligofructose and inulin, selective fermentable chicory fructans, have been shown to stimulate the growth of bifidobacteria, which are regarded as beneficial strains in the colon. studies were designed to evaluate inulin (raftiline) and oligofructose (raftilose) for their potential inhibitory properties against the development of colonic aberrant crypt foci (acf) in rats. acf are putative preneoplastic lesions from which adenomas and carcinomas may develop in the colon. the results of this study ... | 1999 | 10395625 |
| starter culture activity in refrigerated fermented soymilk. | the refrigerated shelf life of soymilk fermented with single cultures of lactobacillus fermentum, l. casei, streptococcus salivarius subsp. thermophilus, and bifidobacterium longum was evaluated. during storage at 4 degrees c for 28 days, the stability of the microflora differed markedly among the starter cultures. after 28 days, the average numbers of s. salivarius subsp. thermophilus decreased by two log cycles to 6.0 x 10(7) cfu/ml, whereas those of l. casei increased gradually by more than t ... | 1999 | 10419278 |