| synthesis of the compatible solutes glucosylglycerol and trehalose by salt-stressed cells of stenotrophomonas strains. | in this study, physiological processes were analysed, which are involved in salt acclimation of two stenotrophomonas species, stenotrophomonas maltophilia strain dsm 50170 and stenotrophomonas rhizophila strain dsm 14405. s. maltophilia accumulated trehalose as the only osmolyte, whereas s. rhizophila produced additionally to trehalose glucosylglycerol (gg). the different spectrum and amounts of compatible solutes in these two strains led to differences in terms of their salt tolerance. the huma ... | 2005 | 15668022 |
| a molecular biological protocol to distinguish potentially human pathogenic stenotrophomonas maltophilia from plant-associated stenotrophomonas rhizophila. | in recent years, the importance of the gram-negative bacterium stenotrophomonas as an opportunistic pathogen as well as in biotechnology has increased. the aim of the present study was to develop new methods for distinguishing between strains closely related to the potentially human pathogenic stenotrophomonas maltophilia and those closely related to the plant-associated stenotrophomonas rhizophila. to accomplish this, 58 strains were characterized by 16s rdna sequencing and amplified ribosomal ... | 2005 | 16232300 |
| volatiles of bacterial antagonists inhibit mycelial growth of the plant pathogen rhizoctonia solani. | bacterial antagonists are bacteria that negatively affect the growth of other organisms. many antagonists inhibit the growth of fungi by various mechanisms, e.g., secretion of lytic enzymes, siderophores and antibiotics. such inhibition of fungal growth may indirectly support plant growth. here, we demonstrate that small organic volatile compounds (vocs) emitted from bacterial antagonists negatively influence the mycelial growth of the soil-borne phytopathogenic fungus rhizoctonia solani kühn. s ... | 2007 | 17180381 |
| level of chemical and microbiological contaminations in chili bo (paste). | the objective of this study was to determine the level of preservatives and microbiological loads in various brands of commercially available chili bo (paste). fifteen different brands of chili bo obtained from the local market and hypermarkets were analyzed for ph, moisture and benzoic acid content, microbiological loads (aerobic, anaerobic, aerobic spores, and fungi), and thermophilic microorganisms. results showed that both moisture content and ph vary among samples. the concentrations of ben ... | 2010 | 20202342 |
| stenotrophomonas panacihumi sp. nov., isolated from soil of a ginseng field. | the study isolated a gram-negative, rod-shaped, non-motile bacterium from the soil of a ginseng field in daejeon, south korea and characterized it to determine its taxonomic position. phylogenetic analysis, based on the 16s rrna gene sequence, revealed that strain mk06(t) belongs to the family xanthomonadacea, and showed the highest degree of sequence similarity to stenotrophomonas rhizophila e-p10(t) (98.6%), xanthomonas campestris lmg 568t (98.0%), stenotrophomonas maltophilia atcc 1d3637(t) ( ... | 2010 | 20221726 |
| screening for endophytic nitrogen-fixing bacteria in brazilian sugar cane varieties used in organic farming and description of stenotrophomonas pavanii sp. nov. | a gram-negative, rod-shaped, non-spore-forming and nitrogen-fixing bacterium, designated icb 89(t), was isolated from stems of a brazilian sugar cane variety widely used in organic farming. 16s rrna gene sequence analysis revealed that strain icb 89(t) belonged to the genus stenotrophomonas and was most closely related to stenotrophomonas maltophilia lmg 958(t), stenotrophomonas rhizophila lmg 22075(t), stenotrophomonas nitritireducens l2(t), [pseudomonas] geniculata atcc 19374(t), [pseudomonas] ... | 2011 | 20495025 |
| identification of stenotrophomonas maltophilia strains isolated from environmental and clinical samples: a rapid and efficient procedure. | aims:ôçé aim of the study is to identify accurately stenotrophomonas maltophilia isolates recovered from environmental and clinical samples. methods and results:ôçé recovery of sten.-ámaltophilia-like isolates from soil samples using the vancomycin, imipenem, amphotericin b (via) selective agar medium enabled distinction of various morphotype colonies. a set of soil and clinical isolates was tested for species identification using different methods. 16s rdna analyses showed the dark green with a ... | 2011 | 21819497 |
| prevention of aflatoxin contamination by a soil bacterium of stenotrophomonas sp. that produces aflatoxin production inhibitors. | a soil bacterium, designated strain no. 27, was found to produce aflatoxin-production inhibitors. the strain was identified as a species of the genus stenotrophomonas, and was found to be closely related to stenotrophomonas rhizophila. two diketopiperazines, cyclo(l-ala-l-pro) and cyclo(l-val-l-pro), were isolated from the bacterial culture filtrate as main active components. these compounds inhibited aflatoxin production of aspergillus parasiticus and aspergillus flavus in liquid medium at conc ... | 2013 | 23449921 |
| a metaproteomic approach dissecting major bacterial functions in the rhizosphere of plants living in serpentine soil. | a metaproteomic approach, based on liquid chromatography-high resolution mass spectrometry (lc-hrms) analysis, was followed to map the major bacterial metabolic functions associated with the rhizosphere of metal-tolerant and metal hyperaccumulator plants, growing in a serpentine soil naturally contaminated by heavy metals such as ni, co and cr. in particular, an "in-house" bacterial protein database was built based on the genera recognised by 16s rdna profiling, then it was used for protein iden ... | 2017 | 28083663 |
| quantitative analysis of volatile metabolites released in vitro by bacteria of the genus stenotrophomonas for identification of breath biomarkers of respiratory infection in cystic fibrosis. | the aim of the present study was to characterize the volatile metabolites produced by genotypically diverse strains of the stenotrophomonas genus in order to evaluate their potential as biomarkers of lung infection by non-invasive breath analysis. volatile organic compounds (vocs) emitted from 15 clinical and five environmental strains belonging to different genogroups of stenotrophomonas maltophilia (n = 18) and stenotrophomonas rhizophila (n = 2) cultured in mueller-hinton broth (mhb) liquid m ... | 2015 | 25830686 |
| using phenotype microarrays in the assessment of the antibiotic susceptibility profile of bacteria isolated from wastewater in on-site treatment facilities. | the scope of the study was to apply phenotype biolog microarray (pm) technology to test the antibiotic sensitivity of the bacterial strains isolated from on-site wastewater treatment facilities. in the first step of the study, the percentage values of resistant bacteria from total heterotrophic bacteria growing on solid media supplemented with various antibiotics were determined. in the untreated wastewater, the average shares of kanamycin-, streptomycin-, and tetracycline-resistant bacteria wer ... | 2017 | 28451946 |
| stenotrophomonas bentonitica sp. nov., isolated from bentonite formations. | a gram-stain negative, rod-shaped, aerobic bacterial strain, bii-r7t, was isolated during a study targeting the culture-dependent microbial diversity occurring in bentonite formations from southern spain. comparative 16s rrna gene sequence analysis showed that bii-r7t represented a member of the genus stenotrophomonas (class gammaproteobacteria), and was related most closely to stenotrophomonas rhizophila e-p10t (99.2 % sequence similarity), followed by stenotrophomonas pavanii icb 89t (98.5 %), ... | 2017 | 28820086 |
| interactions of freshwater cyanobacteria with bacterial antagonists. | cyanobacterial and algal mass development, or blooms, have severe effects on freshwater and marine systems around the world. many of these phototrophs produce a variety of potent toxins, contribute to oxygen depletion, and affect water quality in several ways. coexisting antagonists, such as cyanolytic bacteria, hold the potential to suppress, or even terminate, such blooms, yet the nature of this interaction is not well studied. we isolated 31 cyanolytic bacteria affiliated with the genera pseu ... | 2017 | 28115385 |
| optimization of crude oil and pahs degradation by stenotrophomonas rhizophila kx082814 strain through response surface methodology using box-behnken design. | the present paper describes the process optimization study for crude oil degradation which is a continuation of our earlier work on hydrocarbon degradation study of the isolate stenotrophomonas rhizophila (pm-1) with genbank accession number kx082814. response surface methodology with box-behnken design was used to optimize the process wherein temperature, ph, salinity, and inoculum size (at three levels) were used as independent variables and total petroleum hydrocarbon, biological oxygen deman ... | 2016 | 28116165 |
| the impact of bacteria of circulating water on apatite-nepheline ore flotation. | a new phenomenon has been identified and studied-the impact of bacteria on the benefication process of non-sulphide ores using circulating water supply-a case study of apatite-nepheline ore. it is shown that bacteria deteriorate the floatability of apatite due to their interaction with active centres of calcium-containing minerals and intense flocculation, resulting in a decrease of the flotation process selectivity thus deteriorating the quality of concentrate. based on the comparative analysis ... | 2012 | 22320692 |
| characterization of aerobic oil and grease-degrading bacteria in wastewater. | a bacterial consortium that degrades cooking oil (co) has been isolated in wastewater (ww) samples, by enrichment in olive co. this consortium could degrade 90% of co within 7-9 days (from an initial 1% [w/v]), and it is more active at alkaline conditions. the 16s ribonucleic acid (rna) gene analysis showed that it contains five bacterium species: stenotrophomonas rhizophila, sphingobacterium sp., pseudomonas libanensis, pseudomonas poae and pseudomonas aeruginosa. this consortium can degrade th ... | 2017 | 27426954 |
| high prevalence of biofilm synergy among bacterial soil isolates in cocultures indicates bacterial interspecific cooperation. | biofilms that form on roots, litter and soil particles typically contain multiple bacterial species. currently, little is known about multispecies biofilm interactions and few studies have been based on environmental isolates. here, the prevalence of synergistic effects in biofilm formation among seven different soil isolates, cocultured in combinations of four species, was investigated. we observed greater biofilm biomass production in 63% of the four-species culture combinations tested than in ... | 2015 | 24936766 |
| distinct gene expression profile of xanthomonas retroflexus engaged in synergistic multispecies biofilm formation. | it is well known that bacteria often exist in naturally formed multispecies biofilms. within these biofilms, interspecies interactions seem to have an important role in ecological processes. little is known about the effects of interspecies interactions on gene expression in these multispecies biofilms. this study presents a comparative gene expression analysis of the xanthomonas retroflexus transcriptome when grown in a single-species biofilm and in dual- and four-species consortia with stenotr ... | 2017 | 27505346 |
| the ability of soil bacteria to receive the conjugative incp1 plasmid, pkjk10, is different in a mixed community compared to single strains. | horizontal gene transfer by conjugation is common among bacterial populations in soil. it is well known that the host range of plasmids depends on several factors, including the identity of the plasmid host cell. in the present study, however, we demonstrate that the composition of the recipient community is also determining for the dissemination of a conjugative plasmid. we isolated 15 different bacterial strains from soil and assessed the conjugation frequencies of the incp1 plasmid, pkjk10, b ... | 2013 | 23106414 |
| effects of illuminance and nutrients on bacterial photo-physiology of hydrocarbon degradation. | bacterial photophysiology was previously limited to photoautotrophs. the discovery of bacteriophytochromes in non-photoautotrophs raised a question whether these non-photoautotrophs are affected by the presence or absence of light? in this research work for the first time, bacterial hydrocarbon degradation and biomass production was studied under the influence of nutrients, illuminance (light flux) and time. an experimental model was designed, with six isolated bacterial strains (pseudomonas poa ... | 2016 | 27039061 |
| root-microbe systems: the effect and mode of interaction of stress protecting agent (spa) stenotrophomonas rhizophila dsm14405(t.). | stenotrophomonas rhizophila has great potential for applications in biotechnology and biological control due to its ability to both promote plant growth and protect roots against biotic and a-biotic stresses, yet little is known about the mode of interactions in the root-environment system. we studied mechanisms associated with osmotic stress using transcriptomic and microscopic approaches. in response to salt or root extracts, the transcriptome of s. rhizophila dsm14405(t) changed drastically. ... | 2013 | 23717321 |
| soil-derived microbial consortia enriched with different plant biomass reveal distinct players acting in lignocellulose degradation. | here, we investigated how different plant biomass, and-for one substrate-ph, drive the composition of degrader microbial consortia. we bred such consortia from forest soil, incubated along nine aerobic sequential - batch enrichments with wheat straw (ws1, ph 7.2; ws2, ph 9.0), switchgrass (sg, ph 7.2), and corn stover (cs, ph 7.2) as carbon sources. lignocellulosic compounds (lignin, cellulose and xylan) were best degraded in treatment sg, followed by cs, ws1 and ws2. in terms of composition, th ... | 2015 | 26487437 |
| differences between the rhizosphere microbiome of beta vulgaris ssp. maritima-ancestor of all beet crops-and modern sugar beets. | the structure and function of the plant microbiome is driven by plant species and prevailing environmental conditions. effectuated by breeding efforts, modern crops diverge genetically and phenotypically from their wild relatives but little is known about consequences for the associated microbiota. therefore, we studied bacterial rhizosphere communities associated with the wild beet b. vulgaris ssp. maritima grown in their natural habitat soil from coastal drift lines (cs) and modern sugar beets ... | 2014 | 25206350 |
| effects of bacterial inoculants on the indigenous microbiome and secondary metabolites of chamomile plants. | plant-associated bacteria fulfill important functions for plant growth and health. however, our knowledge about the impact of bacterial treatments on the host's microbiome and physiology is limited. the present study was conducted to assess the impact of bacterial inoculants on the microbiome of chamomile plants chamomilla recutita (l.) rauschert grown in a field under organic management in egypt. chamomile seedlings were inoculated with three indigenous gram-positive strains (streptomyces subru ... | 2014 | 24600444 |
| the plant-associated bacterium stenotrophomonas rhizophila expresses a new enzyme for the synthesis of the compatible solute glucosylglycerol. | the rhizobacterium stenotrophomonas rhizophila accumulates the compatible solutes glucosylglycerol (gg) and trehalose under salt stress conditions. the complete gene for the gg synthesis enzyme was cloned and sequenced. this enzyme from s. rhizophila represented a novel fusion protein composed of a putative c-terminal gg-phosphate synthase domain and an n-terminal putative gg-phosphate phosphatase domain, which was named ggpps. a similar gene was cloned from pseudomonas sp. strain oa146. the ggp ... | 2008 | 18586931 |
| highly sensitive determination of ectoine and other compatible solutes by anion-exchange chromatography and pulsed amperometric detection. | in saline media prokaryotes compensate for the osmotic pressure of the surrounding medium by producing osmolytes. although these osmolytes or osmoprotectors have quite diverse structures, most of them can be determined by anion-exchange chromatography combined with integrated pulsed amperometric detection. this technique offers the advantages of very high sensitivity and new opportunities to determine ectoine and 5-hydroxyectoine-two important osmolytes -after hydrolytic cleavage of the pyrimidi ... | 2003 | 12851735 |
| stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties. | a polyphasic taxonomic study was performed on 16 stenotrophomonas strains from environmental and clinical sources. a group of three plant-associated isolates were shown to be phenotypically different from the other strains. this group formed a separate physiological cluster (b1) with 42% heterogeneity to the other isolates. the defining characteristics of the new species were as follows: growth at 4 degrees c and the absence of growth at 40 degrees c; the utilization of xylose as a carbon source ... | 2002 | 12508851 |
| low-abundant species facilitates specific spatial organization that promotes multispecies biofilm formation. | microorganisms frequently co-exist in matrix-embedded multispecies biofilms. within biofilms, interspecies interactions influence the spatial organization of member species, which likely play an important role in shaping the development, structure and function of these communities. here, a reproducible four-species biofilm, composed of stenotrophomonas rhizophila, xanthomonas retroflexus, microbacterium oxydans and paenibacillus amylolyticus, was established to study the importance of individual ... | 2017 | 28618083 |
| [isolation, identification and characterization of 68 protease-producing bacterial strains from the arctic]. | we screened and identified protease-producing bacterial strains from the arctic, the results would help find cold-adapted protease. | 2013 | 24195377 |
| [identification of bacterial strain ge15 and its controlling effect on ginseng diseases]. | based on previous results of 16s rdna sequence homologuous and results of physic-biochemical indexes and morphological characteristics in the present work, bacterial strain ge15 isolated from roots of ginseng plants was identified as stenotrophomonas rhizophila. confronting incubation results showed that, strain ge15 inhibited the growth of alternaria panax, phytophthora cactorum, and cylindrocapon destructans significantly, and the width of inhibition zone was 13.3, 24.0, 12.0 mm, respectively. ... | 2014 | 25898572 |