| [present utilization situation of main fishery resources in zhoushan fishing ground]. | based on principal component analysis, the present utilization situation of main fishery resources in zhoushan fishing ground were studied. the results showed that the nine main fishery resources in zhoushan fishing ground could be divided into four categories, i.e., pseudosciaena crocea and sepiella maindroni rochebrune were at the degenerating stage; trichiurus haumela, pneumatophorus japonicus and decapterus maruadsi at the fully-utilized stage; muraenesox cinereus, pseudosciaena polyactis an ... | 2003 | 12920904 |
| outbreaks of an iridovirus disease in maricultured large yellow croaker, larimichthys crocea (richardson), in china. | | 2003 | 14653319 |
| a preliminary risk assessment of trace elements accumulated in fish to the indo-pacific humpback dolphin (sousa chinensis) in the northwestern waters of hong kong. | in order to assess the potential risks associated with consumption of contaminated prey items to the indo-pacific humpback dolphin (sousa chinensis), fish species (collichthys lucida, pseudosciaena crocea, johnius sp., thryssa sp., mugil sp. and trichiurus sp.) representing the main food items of the dolphin were collected from the northwestern waters of hong kong, including the sha chau and lung kwu chau marine park, which form the main habitat of the dolphin in hong kong. within these waters, ... | 2004 | 15234160 |
| nocardiosis in large yellow croaker, larimichthys crocea (richardson). | an epizootic in seawater-cage reared large yellow croaker, larimichthys crocea, in china was caused by a nocardia sp. from august to october 2003. the cumulative mortality rate was 15% and the diseased fish were 16 months old with individual length varying from 25 to 30 cm. multiple, white nodules, 0.1-0.2 cm in diameter, were scattered on the heart, spleen and kidney. the morphology of isolated bacteria from lowenstein-jensen medium and tryptic soy agar was bead-like or long, slender, filamento ... | 2005 | 15960657 |
| quantitative detection of a marine fish iridovirus isolated from large yellow croaker, pseudosciaena crocea, using a molecular beacon. | a real-time polymerase chain reaction (pcr) assay utilizing a molecular beacon for the quantitative detection of a marine fish iridovirus isolated from large yellow croaker, pseudosciaena crocea (lyciv), was developed, which involved the amplification of a 122bp dna fragment from a conserved region of lyciv atpase gene. the specific probe consisting of two short arm and a central loop sequences complementary to the target amplicon was characterized with respect to its efficiency of quenching (e( ... | 2006 | 16310867 |
| cloning and expression analysis of interferon-gamma-inducible-lysosomal thiol reductase gene in large yellow croaker (pseudosciaena crocea). | in mammals, interferon-gamma-inducible-lysosomal thiol reductase (gilt) has been demonstrated to play a key role in the processing and presentation of mhc class ii-restricted antigen (ag) by catalyzing disulfide bond reduction, thus unfolding native protein ag and facilitating subsequent cleavage by proteases. here, we reported the cloning of a gilt gene homologue from the spleen of large yellow croaker, a marine fish (lycgilt). the full-length cdna of lycgilt gene is 1033 nucleotides (nt) encod ... | 2006 | 16478632 |
| a preliminary risk assessment of organochlorines accumulated in fish to the indo-pacific humpback dolphin (sousa chinensis) in the northwestern waters of hong kong. | the indo-pacific humpback dolphin is considered threatened due to several factors including pollution in hong kong and the risks due to consumption of fish tainted with polychlorinated biphenyls (pcbs) and organochlorine pesticides were assessed. six species of fish collichthys lucida, pseudosciaena crocea, johnius sp., thryssa sp., mugil sp. and trichiurus sp., which comprise the main prey species of humpback dolphins were collected for analyses. risks due to total pcbs, total teqs, pcb 118 and ... | 2006 | 16516360 |
| identification and characterization of a novel gene encoding an rgd-containing protein in large yellow croaker iridovirus. | many virus-encoded rgd-containing proteins have been reported to play important roles in virus attachment and entry. here we report the identification and functional characterization of a gene encoding an rgd-containing protein (037l) from large yellow croaker iridovirus (lyciv), a causative agent of epizootics among large yellow croaker, pseudosciaena crocea. the 037l gene is 1347 bp long and encodes a protein of 449 amino acids containing a biologically active rgd tri-peptide predicted with su ... | 2006 | 16916530 |
| effects of dietary beta-1, 3 glucan on innate immune response of large yellow croaker, pseudosciaena crocea. | the present study was conducted to investigate the effects of dietary beta-1, 3 glucan on the innate immune response and protection against vibrio harveyi infection in large yellow croaker, pseudosciaena crocea. a basal diet was supplemented with 0% (control), 0.09% (low) and 0.18% (high) beta-1, 3 glucan to formulate three experimental diets. each diet was randomly allocated to triplicate groups of fish in floating sea cages (1.5 x 1.5 x 2.0m), and each cage was stocked with 100 fish (initial a ... | 2007 | 16928452 |
| hepatic and muscle expression of thyroid hormone receptors in association with body and muscle growth in large yellow croaker, pseudosciaena crocea (richardson). | the role of thyroid hormone (th) and its receptors (trs) in the regulation of body growth and muscle accretion is well established in mammals and birds, whereas the involvement of ths and trs in fish growth, especially during the muscle accretion period of juvenile-adult transition, is unknown. this study describes the cloning of the partial cdna sequences of tralpha and trbeta in large yellow croaker, pseudosciaena crocea (richardson) and the patterns of tralpha and trbeta mrna expression in li ... | 2007 | 17324424 |
| cloning, expression and immunogenicty analysis of five outer membrane proteins of vibrio parahaemolyticus zj2003. | genes of five outer membrane proteins of vibrio parahaemolyticus zj2003, including ompw, ompv, ompk, ompu and tolc, were cloned and expressed as n-terminal his(6)-tagged proteins in escherichia coli. the recombinant fusion proteins were purified with nickel chelate affinity chromatography. to analyze the immunogenicity of these proteins, large yellow croaker (pseudosciaena crocea) were immunized by intraperitoneal injection. antibody response was assessed by method of enzyme-linked immunosorbent ... | 2007 | 17451968 |
| expression and immunogenicity analysis of two iron-regulated outer membrane proteins of vibrio parahaemolyticus. | genes of two iron-regulated outer membrane proteins of vibrio parahaemolyticus zj2003, a pathogenic strain isolated from large yellow croaker (pseudosciaena crocea), psua and pvua, were cloned and expressed as n-terminal his(6)-tagged proteins in escherichia coli bl(21)(de(3)). the recombinant fusion proteins were purified with nickel chelate affinity chromatography. to analyze the immunogenicity of the proteins, groups of large yellow croaker were immunized with the purified recombinant psua, p ... | 2007 | 17928925 |
| characterization of ompk, gapdh and their fusion ompk-gapdh derived from vibrio harveyi outer membrane proteins: their immunoprotective ability against vibriosis in large yellow croaker (pseudosciaena crocea). | to investigate the immunoprotection of three recombinant proteins derived from the vibrio harveyi outer membrane proteins (omps) ompk, glyceraldehyde-3-phosphate dehydrogenase (gapdh) and their fusion ompk-gapdh as vaccine candidates from vibriosis of large yellow croaker (pseudosciaena crocea). | 2007 | 17953570 |
| molecular cloning of leucocyte cell-derived chemotaxin-2 gene in croceine croaker (pseudosciaena crocea). | leucocyte cell-derived chemotaxin-2 (lect2) was originally demonstrated to have a chemotactic activity against human neutrophils in vitro. current evidence suggests that lect2 may be a multifunctional protein involved in cell growth, differentiation and autoimmune. a full-length cdna clone of the lect2 gene, 595bp in size, was isolated from the fish croceine croaker (pseudosciaena crocea). it's 3'-utr was much shorter (112nts) than that of trout lect2 gene (210nts). its deduced amino acid sequen ... | 2008 | 18155922 |
| molecular cloning and expression analysis of interferon-inducible transmembrane protein 1 in large yellow croaker pseudosciaena crocea. | in human cells, interferon-inducible transmembrane protein 1 (ifitm1) is a component of protein complexes involved in homotypic adhesion and the transduction of antiproliferative signals. here, we reported the cloning of an ifitm1 homologue from the spleen of large yellow croaker pseudosciaena crocea (lycifitm1). the complete cdna of lycifitm1 is 734 nucleotides (nt) encoding a protein of 124 amino acids (aa), with a putative molecular weight of 13.6 kda. the deduced lycifitm1 protein is signifi ... | 2008 | 18281101 |
| cloning and expression of vibrio harveyi ompk* and gapdh* genes and their potential application as vaccines in large yellow croakers pseudosciaena crocea. | vibrio harveyi is a causative agent of vibriosis in the large yellow croaker pseudosciaena crocea and causes severe losses to the aquaculture industry in china. the vaccines based on the outer membrane proteins (omps) of the pathogens are considered to be the optimum intervention for this disease. in this study, two v. harveyi omp genes, ompk* and glyceraldehyde-3-phosphate dehydrogenase (gapdh*), were cloned, sequenced, and characterized. the recombinant proteins (r-ompk and r-gapdh) were expre ... | 2008 | 18536497 |
| influence of dietary conjugated linoleic acid on growth, fatty acid composition and hepatic lipogenesis in large yellow croaker (pseudosciaena crocea r.). | we examined the effects of conjugated linoleic acid (cla) on growth, fatty acid composition and enzyme activity of fatty acid oxidation in the liver of large yellow croaker. we divided 1600 fish (average initial weight 150 g) into 4 groups and reared them in 8 cages. four dietary treatments were formulated to contain 0%, 1%, 2% and 4% (w/w) cla, respectively. the fish were fed for 10 weeks ad libitum twice daily. we found that the dietary cla had no effect on growth, biometric parameters and who ... | 2008 | 18763301 |
| portal of entry for pathogenic vibrio alginolyticus into large yellow croaker pseudosciaena crocea, and characteristics of bacterial adhesion to mucus. | the portal of entry for pathogenic vibrio alginolyticus into large yellow croaker pseudosciaena crocea is via the intestinal tract rather than gill or skin according to the kinetics of the bacterial adhesion to different mucus. the different effects on adhesion caused by proteolytic enzymes and heat treatment might be due to the different chemical compositions of mucus. adhesion of v. alginolyticus to mucus depends on concerted action of bacterial surface structures such as cell-surface proteins ... | 2008 | 18814543 |
| cloning and expression analysis of two alternative splicing toll-like receptor 9 isoforms a and b in large yellow croaker, pseudosciaena crocea. | toll-like receptors (tlrs) are the archetypal pattern-recognition receptors in sensing foreign pathogens. in this report, two alternative splicing isoforms of tlr9 (named pctlr9a and pctlr9b) cdna were cloned from the large yellow croaker, pseudosciaena crocea. the full-length cdna of pctlr9a was of 3637bp, including a 5'-terminal untranslated region (utr) of 111bp, 3'-terminal utr of 355bp and an open reading frame (orf) of 3171bp encoding a polypeptide of 1056 amino acids. however, the full-le ... | 2008 | 18824108 |
| molecular and functional characterization of a novel cc chemokine in large yellow croaker (pseudosciaena crocea). | a full-length cc chemokine cdna was isolated from large yellow croaker (pseudosciaena crocea) by expressed sequence tag (est) analysis (lyccc2). its open reading frame (orf) is 282 nucleotides (nt), encoding a polypeptide of 93 amino acids (aa). the deduced lyccc2 contains a 20-aa signal peptide and a 73-aa mature polypeptide, which possesses the typical arrangement of four cysteines as found in other known cc chemokines (c(34), c(35), c(61) and c(75)). it shares 17.4-40.4% and 10.0-24.5% aa seq ... | 2008 | 18835451 |
| molecular characterization and expression analysis of beta2-microglobulin in large yellow croaker pseudosciaena crocea. | beta(2)-microglobulin (beta(2)m), a protein necessary for proper folding, peptide binding, and surface display of class i antigens plays an important role in immune response. the full-length cdna containing beta(2)m was cloned from the spleen cdna library of large yellow croaker pseudosciaena crocea (pscr-beta ( 2 ) m) by expressed sequence tag (est) analysis. the pscr-beta ( 2 ) m is 926 nucleotides (nt) long, including an open reading frame (orf) of 348 nt encoding a polypeptide of 116 amino a ... | 2009 | 18853280 |
| characterization of the bpi-like gene from a subtracted cdna library of large yellow croaker (pseudosciaena crocea) and induced expression by formalin-inactivated vibrio alginolyticus and nocardia seriolae vaccine challenges. | one expressed sequence tag (est 64lf004 clone), which is from the subtracted cdna library of the head kidney of large yellow croaker (pseudosciaena crocea) stimulated with peptidoglycan (pg) by suppression subtractive hybridization (ssh) method, was cloned using race-pcr. the full length cdna, which possesses typical structural features of a signal peptide, a conserved lps binding domain and two bactericidal permeability-increasing (bpi) motifs as in higher vertebrates, was identified as a novel ... | 2008 | 18952461 |
| molecular cloning and expression of myd88 in large yellow croaker, pseudosciaena crocea. | myeloid differentiation factor 88 (myd88) is an adaptor protein involved in the interleukin-1 receptor and toll-like receptor-induced activation of nuclear factor-kappab (nf-kappab). in this report, the full-length cdna of myd88 was cloned from the large yellow croaker, pseudosciaena crocea. it was of 1574 bp, including a 5'-terminal untranslated region (utr) of 89 bp, a 3'-terminal utr of 621bp and an open reading frame (orf) of 864 bp encoding a polypeptide of 287 amino acids. it contained a t ... | 2009 | 19049879 |
| cloning and expression of a hepcidin gene from a marine fish (pseudosciaena crocea) and the antimicrobial activity of its synthetic peptide. | hepcidin gene is widely expressed in various fish, suggesting that this antimicrobial peptide is a very important component in the innate immune system. large yellow croaker (pseudosciaena crocea) is one of the important economic species of marine-cultured fish but knowledge of its innate immune mechanism is lacking. in this study, we characterize a p. crocea hepcidin gene named as pc-hepc. it consists of an open reading frame of 258 bases encoding 85 amino acids and has a conserved sequence in ... | 2009 | 19150638 |
| an interaction between leukocyte cell-derived chemotaxin 2 and transferrin of ayu, plecoglossus altivelis. | yeast two-hybrid (y2h) screens were used to test for interactions between leukocyte cell-derived chemotaxin 2 (lect2) and a liver cdna expression library of ayu, plecoglossus altivelis. of the 9 independent interacting clones identified, 7 were identical and closely related to transferrin (tf) genes of fish, while the other two were related to c-type lectin genes. the interaction between ayu tf (atf) and ayu lect2 (alect2) was confirmed by in vitro co-immunoprecipitation of the two proteins. y2h ... | 2009 | 19223015 |
| isolation and characterization of a hepcidin peptide from the head kidney of large yellow croaker, pseudosciaena crocea. | large yellow croaker (pseudosciaena crocea) is one of the most important marine cultured fish in china. acidic extracts of five tissues of large yellow croaker showed strong anti-vibrio alginolyticus activity. acidic extract of head kidney tissue was subjected to heat-treatment in boiling water, and solid-phase extraction on sep-pak c(18) cartridge. it was found that the antibacterial substances were heat stable, and 20% acetonitrile effluent exhibited strong antibacterial activity. active extra ... | 2009 | 19344770 |
| identification of immune responsible fibrinogen beta chain in the liver of large yellow croaker using a modified annealing control primer system. | in this article, we used a modified acp system (macp) developed in our laboratory to analyze differentially expressed genes in the liver of large yellow croaker, pseudosciaena crocea (richardson). by using 20 pairs of macps, 7 differentially expressed genes were obtained. one of the genes we identified encodes for a fibrinogen beta chain (fgb). the full-length cdna of fgb was 1645 bp, including 5 bp of 5' untranslated region (5'-utr), 1479 bp of open reading frame (orf), and 161 bp of 3'-utr. th ... | 2009 | 19422918 |
| cloning and molecular characterization of two complement bf/c2 genes in large yellow croaker (pseudosciaena crocea). | complement components factor b and c2 are two crucial proteases in the alternative pathway (ap) and classical pathway (cp). two bf/c2 cdnas, lycbf/c2a and lycbf/c2b were isolated from the large yellow croaker (pseudosciaena crocea) by suppression subtractive hybridization (ssh) and rapid amplification of cdna ends (race). through sequence alignment and computer 3d modeling analysis, we found that both of the deduced proteins contain three complement control protein (ccp) modules, a von willebran ... | 2009 | 19490942 |
| cloning and molecular characterization of lycc1inh genes in large yellow croaker (pseudosciaena crocea). | complement component 1 inhibitor (c1inh) is a crucial protein in controlling activation of many plasma mediator pathways and can directly interact with gram negative bacteria. the full-length cdna of lycc1inh gene was identified from the large yellow croaker. it is of 2046 nucleotides (nt) encoding a protein of 599 amino acids, with a 5'-untranslated region of 99 nt and a 3'-untranslated region of 147 nt including the poly (a) tail. the deduced protein contains a c-terminal serpin (serine protea ... | 2010 | 19761847 |
| gene cloning and characterization of ferritin h and m subunits from large yellow croaker (pseudosciaena crocea). | ferritin, an iron-binding protein, plays a role in host immune response to invading stimulus. two ferritin subunit cdnas, lycfh and lycfm, were identified from large yellow croaker, pseudosciaena crocea. analysis of the lycfh and lycfm 5'-utr showed that both had a putative iron responsive element (ire). amino acid sequence alignment and phylogenetic analysis indicated that lycfh and lycfm had high identity to h and m type subunit of vertebrate ferritins respectively, while both of them showed t ... | 2010 | 20034577 |
| peroxiredoxin iv regulates pro-inflammatory responses in large yellow croaker (pseudosciaena crocea) and protects against bacterial challenge. | in this study, we applied a comparative proteomic approach to the analysis of differentially expressed proteins in the spleens of large yellow croaker following treatment with an inactivated trivalent bacterial vaccine. twenty-four altered proteins were identified by maldi-tof or maldi-tof-tof, including immune-related proteins, antioxidant proteins, signal transducers, protein biosynthesis and catabolism modulators, and carbonic anhydrases. three prx family members, namely, prx i, prx ii, and p ... | 2010 | 20099887 |
| cloning, mrna expression, and recombinant expression of peptidoglycan recognition protein ii gene from large yellow croaker (pseudosciaena crocea). | peptidoglycan recognition protein ii (pglyrp2) is a type of pattern recognition receptor (prr) that has amidase activity and is structurally conserved through evolution. however, their contributions in immune defense are different between mammal pglyrp2 and its counterpart in insects. hitherto, fish pglyrp2 was poorly known in its structure, expression pattern and its contribution in immune defense. in present study, the pglyrp2 gene of the large yellow croaker (pseudosciaena crocea) was cloned ... | 2010 | 20349278 |
| molecular characterization of ran gene up-regulated in large yellow croaker (pseudosciaena crocea) immunity. | rangtpase, one family of small g protein superfamily, has been widely demonstrated to be involved in transport system between cytoplasm and nucleus. however the knowledge about the function of rangtpase in immunity remains limited. in this report, ran gene (named lycran) cdna was cloned from the large yellow croaker, pseudosciaena crocea, a marine fish. the full-length cdna of lycran was of 1033 bp, including a 5'-terminal untranslated region (utr) of 43 bp, 3'-terminal utr of 338 bp and an open ... | 2010 | 20447461 |
| transcriptome and expression profiling analysis revealed changes of multiple signaling pathways involved in immunity in the large yellow croaker during aeromonas hydrophila infection. | the large yellow croaker (pseudosciaena crocea) is an economically important marine fish in china suffering from severe outbreaks of infectious disease caused by marine bacteria such as aeromonas hydrophila (a. hydrophila), resulting in great economic losses. however, the mechanisms involved in the immune response of this fish to bacterial infection are not fully understood. to understand the molecular mechanisms underlying the immune response to such pathogenic bacteria, we used high-throughput ... | 2010 | 20858287 |
| molecular cloning and functional characterization of a rabgtpase in large yellow croaker (pseudosciaena crocea). | the molecular mechanisms of the immune system against pathogens in large yellow croaker (pseudosciaena crocea) are not well known, despite its economic importance as an aquaculture species. in this investigation, a rab gene (named as lycrab gene) was obtained from this fish, which exhibited high homology with rab8 of other species. it was expressed in escherichia coli, and the specific antibody was raised using the purified fusion protein (gst-lycrab). the lycrab protein, containing characterist ... | 2010 | 21112380 |
| characterization of a racgtpase up-regulated in the large yellow croaker pseudosciaena crocea immunity. | the rac proteins are members of the rho family of small g proteins and are implicated in the regulation of several pathways, including those leading to cytoskeleton reorganization, gene expression, cell proliferation, cell adhesion and cell migration and survival. in this investigation, a rac gene (named as lycrac gene) was obtained from the large yellow croaker and it was expressed in escherichia coli and purified. subsequently the specific antibody was raised using the purified fusion protein ... | 2010 | 21130170 |
| molecular cloning and characterization of caspase-3 in large yellow croaker (pseudosciaena crocea). | caspases-3, a member of the cysteine-aspartic acid protease (caspase) family, plays critical roles in the execution of apoptotic pathway. in this study, a caspase-3 homologue was cloned and characterized from large yellow croaker (pseudosciaena crocea). the full-length cdna of large yellow croaker caspase-3 (lyccasp3) is 2222bp with an open reading frame of 858 bp encoding a polypeptide of 285 amino acids (aa). lyccasp3 exhibited a conserved caspase-3 architecture including a prodomain, a large ... | 2011 | 21288491 |
| characterization of toll-like receptor 3 gene in large yellow croaker, pseudosciaena crocea. | toll-like receptor 3 (tlr3) plays an important role in innate immune responses. in this report, the full-length cdna sequence and genomic structure of pseudosciaena crocea tlr3 (pctlr3) were identified and characterized. the full-length cdna of pctlr3 was of 3384bp, including a 5'-terminal untranslated region (utr) of 65bp, a 3'-terminal utr of 589bp and an open reading frame (orf) of 2730bp encoding a polypeptide of 909 amino acid residues. the full-length genome sequence of pctlr3 was composed ... | 2011 | 21549197 |
| a new leukocyte cell-derived chemotaxin-2 from marine fish grouper, epinephelus coioides: molecular cloning and expression analysis. | leukocyte cell-derived chemotaxin-2 (lect2) is a multifunctional protein involved in cell growth, differentiation and autoimmunity. in this study, a new leukocyte cell-derived chemotaxin-2 (eclect2) gene was cloned from grouper, epinephelus coioides, by rapid amplification of cdna ends (race) pcr. the full-length cdna sequence of eclect2 was 595 bp in size, containing a 5'-untranslated region (utr) of 44 bp and a 3'-utr of 83 bp. the deduced protein sequence of the open reading frame (465 bp) sh ... | 2011 | 21763428 |
| Molecular characterization and tissue distribution of ferritin M in kelp grouper, Epinephelus bruneus. | This study, reports the identification and analysis of ferritin M chain, from kelp grouper, Epinephelus bruneus (EbFerM); it comprises 1004 base pair (bp), including 528bp open reading frame (ORF) which encodes 176 amino acid (aa) residues; the calculated molecular weight is 20kDa. The 5'-untranslated region (UTR) possesses 476bp proceeded by a putative Iron Regulatory Element (IRE). Pair wise alignments showed that EbFerM shared 94% identity with that of Larimichthys crocea and Sciaenops. It is ... | 2011 | 21885330 |
| [selection of cross-protective antigens from outer membrane proteins of three pathogenic vibrios isolated from infected large yellow croaker (pseudosciaena crocea)]. | vibrios are universal conditioned-pathogenic bacteria in marine culture environment, and the outbreak of vibrio disease resulted in a serious damage to aquaculture. considering that vibrio disease in aquatic species, especially fishes, usually originated from mixed infection of different species (serotypes or subspecies) of vibrios, it is important to select the potential cross-protective protein antigens as candidates of polyvalent or combined vaccines. in present research, several strains of v ... | 2012 | 23593870 |
| the effect of an adding histidine on biological activity and stability of pc-pis from pseudosciaena crocea. | pc-pis is a novel piscidin-like antimicrobial polypeptide that was identified in pseudosciaena crocea. although active against most bacteria tested, pc-pis was inactive against aeromonas hydrophila and pseudomonas aeruginosa. the pc-pis analogue pc-pis-his was designed by adding a histidine residue at the carboxyl terminal. pc-pis-his demonstrated a more broad-spectrum and stronger antimicrobial activity against a representative set of microorganisms and more potent antiparasitic activity agains ... | 2013 | 24349477 |
| in vivo effects of pichia pastoris-expressed antimicrobial peptide hepcidin on the community composition and metabolism gut microbiota of rats. | hepcidin, one kind of antimicrobial peptides, is one of the promising alternatives to antibiotics with broad spectrum of antimicrobial activity. hepcidins cloned from different kinds of fishes have been produced using exogenous expression systems, and their in vitro antimicrobial effects have been verified. however their in vivo effects on gut microbiota and gut health of hosts remain unclear. here we performed a safety study of hepcidin so that it can be used to reduce microbial contaminations ... | 2016 | 27768776 |
| transcriptome analysis reveals comprehensive insights into the early immune response of large yellow croaker (larimichthys crocea) induced by trivalent bacterial vaccine. | vaccination is an effective and safe strategy for combating bacterial diseases in fish, but the mechanisms underlying the early immune response after vaccination remain to be elucidated. in the present study, we used rna-seq technology to perform transcriptome analysis of spleens from large yellow croaker (larimichthys crocea) induced by inactivated trivalent bacterial vaccine (vibrio parahaemolyticus, vibrio alginolyticus and aeromonas hydrophila). a total of 2,789 or 1,511 differentially expre ... | 2017 | 28135311 |
| induction of type i interferons in response to bacterial stimuli in large yellow croaker larimichthys crocea. | in addition to the crucial roles in coordinating antiviral immune responses, type i interferons (ifns) also play a role in the host immunity against bacterial pathogens. our previous study identified two type i ifns from large yellow croaker larimichthys croaea(lc), lcifnd and lcifnh, and showed their strong induction by poly(i:c) and antiviral activities. in the present study, both lcifnd and lcifnh were found to be rapidly induced in head kidney and spleen by mixed bacteria of vibrio alginolyt ... | 2017 | 28119146 |
| molecular characterization and functional analysis of a piscidin gene in large yellow croaker (larimichthys crocea). | the piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. in this study, we cloned piscidin-5-like type 3 (lcpis5lt3) in large yellow croaker (larimichthys crocea). multiple alignments with other known piscidins revealed amino acid conservation throughout the fish, especially at the signal peptide (22 amino acids). the phylogenetic tree confirmed that lcpis5lt3 and large yellow croaker piscidin-5-l ... | 2016 | 28105799 |
| molecular characterization and evolution analysis of five interleukin-17 receptor genes in large yellow croaker larimichthys crocea. | interleukin-17s (il-17s) play critical roles in inflammatory response and host defense against extracellular pathogens. il-17s induce the immune response signaling through the specific il-17 receptors (il-17rs) that consist of five members (il-17ra to e). in the present work, we have identified the five il-17r orthologs (lycil-17rs) from large yellow croaker larimichthys crocea. the deduced protein of each lycil-17r exhibits a typical il-17r domain architecture, including a signal peptide, the e ... | 2016 | 27633682 |
| characterization and functional analysis of a tandem-repeat galectin-9 in large yellow croaker larimichthys crocea. | galectins are a family of endogenous lectins with β-galactosides affinity, playing significant roles in the innate immunity of vertebrates and invertebrates. in this report, a new galectin-9 cdna was identified and characterized in large yellow croaker larimichthys crocea (designated as lcgal-9). the complete cdna sequence of lcgal-9 was 1795 bp, with an open reading frame (orf) of 1032 bp encoding 343 amino acids. the putative lcgal-9 protein contained two carbohydrate recognition domains (crds ... | 2016 | 26997199 |
| a novel c-type lectin, nattectin-like protein, with a wide range of bacterial agglutination activity in large yellow croaker larimichthys crocea. | c-type lectins (ctls) are generally recognized as a superfamily of ca(2+)-dependent carbohydrate-binding proteins, which serve as pattern recognition receptors (prrs) in innate immunity of vertebrates. in this study, the molecular characterization and immune roles of a novel ctl from larimichthys crocea (designated as lcntc) were investigated. lcntc is a novel protein that shared 33%-49% homology with other teleosts ctls. the full-length cdna of lcntc was composed of 859 bp with a 465 bp open re ... | 2016 | 26828263 |
| molecular and functional identification of three interleukin-17a/f (il-17a/f) homologues in large yellow croaker (larimichthys crocea). | the interleukin-17 (il-17) cytokine family plays a central role in the coordination of inflammatory responses. in fish species, three genes that have a similar homology to both il-17a and il-17f were designated il-17a/f1, 2, and 3. in this study, we identified three il-17a/f homologues (lycil-17a/f1, 2, and 3) from large yellow croaker (larimichthys crocea). the deduced lycil-17a/f1 and 3 had four cysteine residues conserved in teleost il-17a/f1 and 3 homologues and shared a domain similar to th ... | 2016 | 26429410 |
| molecular characterization and biological effects of a c-type lectin-like receptor in large yellow croaker (larimichthys crocea). | the c-type lectin-like receptors (ctlrs) play important roles in innate immunity as one type of pattern recognition receptors. here, we cloned and characterized a c-type lectin-like receptor (lycctlr) from large yellow croaker larimichthys crocea. the full-length cdna of lycctlr is 880 nucleotides long, encoding a protein of 215 amino acids. the deduced lycctlr contains a c-terminal c-type lectin-like domain (ctld), an n-terminal cytoplasmic tail, and a transmembrane region. the ctld of lycctlr ... | 2015 | 26690423 |
| molecular characterization and expression analysis of toll-like receptor 2 in response to bacteria in silvery pomfret intestinal epithelial cells. | toll-like receptor 2 (tlr2) has been shown to play a crucial role in the host defense of pathogenic microbes in innate immunity. in this study, the full-length cdna of tlr2 in silvery pomfret (pampus argenteus) was cloned by homology cloning and the rapid amplification of cdna ends (race) technique. the complete cdna sequence of tlr2 was 2932 bp, containing an open reading frame (orf) of 2469 bp encoding 822 amino acids. a multiple alignment analysis of the silvery pomfret tlr2 protein-coding se ... | 2016 | 27574826 |
| identification of endonuclease domain-containing 1 gene in japanese flounder paralichthys olivaceus. | the mrna level of the endonuclease domain-containing 1 gene (jf_endod1) in japanese flounder paralichthys olivaceus kidney was significantly increased after injection of formalin-killed bacteria cells (fkc) in the previous microarray study. endod1 is a member of the dna/rna non-specific nucleases family, and its role in fish immunity has not been reported. the open reading frame of jf_endod1 cdna was 912 bp, encoding 303 amino acids. the first 27 amino acids were predicted to be a signal peptide ... | 2016 | 26784919 |
| the rab5a gene of marine fish, large yellow croaker (larimichthys crocea), and its response to the infection of cryptocaryon irritans. | rab gtpases, members of the ras superfamily, encode monomeric g-proteins. rab proteins regulate key steps in membrane traffic transport and endocytic pathway of host immune responses. rab5a is involved in immune regulation, particularly in t cell migration and macrophage endocytosis in higher vertebrates. however, little is known of the molecular structure of rab5a gene in marine teleost fish species and its expression profile during the parasite infection. in this study, the full-length cdna se ... | 2016 | 27108380 |
| molecular and immune response characterizations of il-6 in large yellow croaker (larimichthys crocea). | interleukin-6 (il-6) is a multifunctional inflammatory cytokine which exists in multiple tissues and cell lines. in the present study, the full-length cdna and the genomic sequence of il-6 (lcil-6) were cloned from large yellow croaker, larimichthys crocea. the full-length cdna of lcil-6 was 1066 base pairs (bp), containing an open reading frame (orf) of 678 bp encoding for 225 amino acids, a 5' untranslated region (utr) of 71 bp and a 3' utr of 317 bp. the predicted lcil-6 protein included a 24 ... | 2016 | 26868214 |
| molecular characterization and biological effects of a cxcl8 homologue in large yellow croaker (larimichthys crocea). | cxcl8 also called interleukin-8, is a cxc-type chemokine that plays a key role in promoting inflammation. three subgroups of cxcl8 homologues have been reported in teleost fish, including cxcl8_l1, cxcl8_l2 and cxcl8_l3. in the present study, we identified a cxcl8 homologue belonging to cxcl8_l1 subgroup (lyccxcl8_l1) in large yellow croaker (larimichthys crocea) that shares low identity to the previously reported large yellow croaker cxcl8 (lyccxcl8). the full-length cdna of lyccxcl8_l1 is 716 ... | 2015 | 25827624 |
| molecular cloning and functional characterization of a qm protein in large yellow croaker (larimichthys crocea). | since it was proposed to be a tumor suppressor in 1991, qm protein has attracted intensive and wide attention in plants, animals and fungi research fields. up to date, however, the function of qm protein in fish immunity remains unknown. in this investigation, a qm gene (named as lycqm gene) was cloned from large yellow croaker (larimichthys crocea), and lycqm protein was expressed in escherichia coli and purified. the lycqm gene was ubiquitously transcribed in multi-tissues, including spleen, m ... | 2015 | 25680268 |
| effect of sypq gene on poly-n-acetylglucosamine biosynthesis in vibrio parahaemolyticus and its role in infection process. | the syp locus includes four genes encoding putative regulators, six genes encoding glycosyltransferases, two encoding export proteins, and six other genes encoding unidentified functional proteins associated with biofilm formation and symbiotic colonization. however, the individual functions of the respective genes remain unclear. amino acid alignment indicates that sypq is presumably involved in biosynthesizing poly-n-acetylglucosamine (pnag), which is proposed to be a critical virulence factor ... | 2014 | 24418930 |
| antimicrobial effect of bayberry leaf extract for the preservation of large yellow croaker (pseudosciaena crocea). | chemical preservatives have been widely used to keep large yellow croaker fresh. however, the potential harm to human health cannot be ignored. this study was undertaken to investigate the antimicrobial effect of bayberry leaf extract and to evaluate the efficacy of this natural product on the preservation of large yellow croaker. | 2014 | 23929386 |
| molecular and expression characterizations of interleukin-8 gene in large yellow croaker (larimichthys crocea). | il-8 plays a crucial role in acute inflammation by recruiting and mediating neutrophils and other cells and in initiating the oxidative burst in neutrophils and inducing wound healing by promoting angiogenesis. in the present study, the full-length cdna and genome sequence of interleukin-8 (lcil-8) were cloned from large yellow croaker larimichthys crocea. the lcil-8 cdna sequence was 931 bp, containing a 118-bp 5'-untranslated region (utr), a 528-bp 3'-utr and a 285-bp open reading frame (orf) ... | 2013 | 23333360 |
| molecular characterization and bioactivity of a cxcl13 chemokine in large yellow croaker pseudosciaena crocea. | a cxcl13-like chemokine cdna was isolated from large yellow croaker (pseudosciaena crocea) by expressed sequence tag (est) analysis (lyccxcl13). the full-length cdna of lyccxcl13 is 796 nucleotides (nt) encoding a protein of 97 amino acids (aa), with a putative molecular weight of 10.7 kda. the deduced lyccxcl13 contains a 24-aa signal peptide and a 73-aa mature polypeptide, which possesses the typical arrangement of four cysteines as found in other known cxc chemokines (c(25), c(27), c(52) and ... | 2010 | 20005294 |
| cloning, characterization and expression analysis of coagulation factor ii gene in grass carp (ctenopharyngodon idella). | here, we characterized the structure and function of the coagulation factor ii (fii) gene in grass carp and determined its role in coagulation mechanisms. the fii gene est was obtained using a constructed splenic transcriptome database; the full-length fii gene sequence was obtained by 3' and 5' race. the open reading frame (orf) of fii was cloned and the full-length gene was found to be 1718 bp, with an orf of 1572 bp; the gene contained a 25 bp 5'-untranslated region (utr) and 108 bp 3'-utr. t ... | 2015 | 26535692 |
| identification and characterization of a phospholipase a1 activity type three secreted protein, pp_exou from pseudomonas plecoglossicida nb2011, the causative agent of visceral granulomas disease in large yellow croaker (larimichthys crocea). | pseudomonas plecoglossicida nb2011, the causative agent of visceral granulomas disease in farmed larimichthys crocea in china, encodes a predicted type three effector pp_exou, a homolog of the cytotoxin exou of pseudomonas aeruginosa. in this study, secretion of pp_exou was tested in various broth, the protein was expressed with the pet30a prokaryotic system, the phospholipase a (pla) activity of the recombinant protein was determined with fluorogenic phospholipid substrates, fusion expression w ... | 2016 | 27734506 |
| spoilage potential characterization of shewanella and pseudomonas isolated from spoiled large yellow croaker (pseudosciaena crocea). | ten strains were isolated from a spoiled large yellow croaker (pseudosciaena crocea). all of them were able to grow aerobically from 4 to 30°c, and reduce trimethylamine-n-oxide to trimethylamine (tma) and produce h2 s except sb01, pf05 and pf07. biochemical characterization and phylogenetic analysis of 16s rrna gene showed that eight h2 s-producing isolates were closely related to shewanella baltica, and two isolates pf05 and pf07 were identified as pseudomonas fluorescens and pseudomonas fragi ... | 2017 | 27747903 |
| visceral granulomas in farmed large yellow croaker, larimichthys crocea (richardson), caused by a bacterial pathogen, pseudomonas plecoglossicida. | an enzootic disease characterized by granulomas in internal organs occurred in cage-farmed large yellow croaker, larimichthys crocea (richardson), in april and november 2010, in ningbo, zhejiang province. one bacterial strain, named xsdhy-p, was isolated from the diseased fish and identified by biochemical characterization, fatty acid methyl ester (fame) analysis and multilocus sequence analysis (mlsa). according to the results obtained from the biochemical tests, fame analysis and phylogenetic ... | 2014 | 25254273 |
| draft genome sequence of pseudomonas plecoglossicida strain nb2011, the causative agent of white nodules in large yellow croaker (larimichthys crocea). | we describe the draft genome sequence of pseudomonas plecoglossicida strain nb2011, the causative agent of white nodules in cultured large yellow croaker (larimichthys crocea) in china. the draft genome sequence of the bacterium consists of 5.41 million bp, with a g+c content of 62.8%. a total of 4,952 genes were identified. | 2013 | 23929479 |
| vaccination efficiency of surface antigens and killed whole cell of pseudomonas putida in large yellow croaker (pseudosciaena crocea). | large yellow croaker (pseudosciaena crocea), a major marine fish aquacultured in the southeastern coastal region of china, has become endangered by the pathogen pseudomonas putida in recent years. p. putida infections occur in low water temperatures when fish reduce food intake, thus oral antibiotic administration is not practical. therefore, vaccination may be the only method to prevent the infection. in the present study, main surface antigens of p. putida, including lipopolysaccharide (lps), ... | 2013 | 23659996 |
| development of a visual loop-mediated isothermal amplification method for rapid detection of the bacterial pathogen pseudomonas putida of the large yellow croaker (pseudosciaena crocea). | in recent years, the large yellow croaker (pseudosciaena crocea), an important marine fish farmed in the coastal areas of zhejiang province, east china, has become severely endangered as a result of the bacterial pathogen pseudomonas putida. this paper reports the development of a visual loop-mediated isothermal amplification (lamp) assay for rapid detection of the pathogen. four primers, f3, b3, fip and bip, were designed on the basis of dna sequence of the rpon gene of p. putida. after optimiz ... | 2012 | 22475582 |
| identification of sea perch (lateolabrax japonicus) ribonucleoprotein ptb-binding 1 involved in antiviral immune response against rgnnv. | rig-i-like receptors (rlrs) can recognize viral rna and initiate innate antiviral response. in earlier studies, we demonstrated that rlrs were implicated in the antiviral immunity against rgnnv in the seawater fish sea perch (lateolabrax japonicus). however, potential regulators of rlrs-mediated signaling pathways involved in rgnnv infection remain unclear. in this study, a novel ribonucleoprotein ptb-binding 1 (raver1) of sea perch (ljraver1) was identified for the first time. the cdna of ljrav ... | 2017 | 27876623 |
| fish trim32 functions as a critical antiviral molecule against iridovirus and nodavirus. | tripartite motif-containing 32 (trim32) has been demonstrated to pay vital roles in cancer, genetic disorders and antiviral immunity. however, the molecular functions of fish trim32 still remained largely unknown. here, a novel trim32 gene from orange spotted grouper (ectrim32) was cloned and characterized. ectrim32 encoded a 685-aa protein which showed 93%, and 60% identity to large yellow croaker (larimichthys crocea) and human (homo sapiens), respectively. amino acid alignment showed that ect ... | 2017 | 27847343 |
| fish trim16l exerts negative regulation on antiviral immune response against grouper iridoviruses. | tripartite motif 16 (trim16), has been demonstrated to act as a tumor suppressor through affecting cell proliferation and migration or tumorigenicity in carcigenesis. however, the roles of trim16 in immune response were unknown up to now. here, we cloned a trim16-like gene (trim16l) from orange spotted grouper (ectrim16l) and investigated its roles in response to virus infection. ectrim16l encoded a 478 amino acid peptide which showed 72% and 29% identity to large yellow croaker (larimichthys cr ... | 2016 | 27815200 |
| ring domain is essential for the antiviral activity of trim25 from orange spotted grouper. | tripartite motif-containing 25 (trim25) has been demonstrated to exert crucial roles in the regulation of innate immune signaling. however, the roles of fish trim25 in antiviral immune response still remained uncertain. here, a novel fish trim25 gene from orange spotted grouper (ectrim25) was cloned and its roles in grouper virus infection were elucidated. ectrim25 encoded a 734-aa protein which shared 68% identity to large yellow croaker (larimichthys crocea). amino acid alignment showed that e ... | 2016 | 27276113 |
| grouper trim13 exerts negative regulation of antiviral immune response against nodavirus. | the tripartite motif (trim)-containing proteins have attracted particular attention to their multiple functions in different biological processes. trim13, a member of the trim family, is a ring domain-containing e3 ubiquitin ligase which plays critical roles in diverse cellular processes including cell death, cancer and antiviral immunity. in this study, a trim13 homolog from orange spotted grouper, epinephelus coioides (ectrim13) was cloned and characterized. the full-length of ectrim13 cdna en ... | 2016 | 27235367 |
| fish trim8 exerts antiviral roles through regulation of the proinflammatory factors and interferon signaling. | the tripartite motif (trim)-containing proteins usually exert important regulatory roles during multiple biological processes. trim8 has been demonstrated to be a ring domain-containing e3 ubiquitin ligase which plays critical roles in inflammation and cancer. in this study, a trim8 homolog from grouper, epinephelus coioides (ectrim8) was cloned, and its effects on fish virus replication were investigated. the full-length ectrim8 cdna encoded a polypeptide of 568 amino acids with 92% identity to ... | 2016 | 27150052 |
| identification and characterization of the melanoma differentiation - associated gene 5 in sea perch, lateolabrax japonicus. | the rig-i-like receptors family is a group of cytosolic rna helicase proteins that can recognize viral rna via binding to pathogen associated molecular pattern motifs within rna ligands. a novel vertebrate rlr counterpart named ljmda5 was firstly identified from the marine fish sea perch lateolabrax japonicus in this study. the full-length cdna of ljmda5 is 3750 bp and encodes a polypeptide of 988 amino acids, containing two n-terminal tandem caspase activation and recruitment domains, a dexh (a ... | 2016 | 27039216 |
| quorum sensing signals affect spoilage of refrigerated large yellow croaker (pseudosciaena crocea) by shewanella baltica. | in this work we investigated the specific spoilage organism (sso) of large yellow croaker (pseudosciaena crocea) stored at 4°c and role of quorum sensing (qs) system of sso isolated from the spoiled fish. according to microbial count and 16s rrna gene of the isolated pure strains, shewanella, mainly shewanella baltica and shewanella putrefaciens, was predominant genera at the end of shelf-life of p. crocea. among shewanella isolates, s.baltica02 was demonstrated as sso in spoilage potential char ... | 2016 | 26519730 |
| identification and characterization of extracellular cyclic dipeptides as quorum-sensing signal molecules from shewanella baltica, the specific spoilage organism of pseudosciaena crocea during 4 °c storage. | quorum-sensing (qs) signaling molecules are able to mediate specific gene expression inside spoilage bacteria in response to population density and thus are implicated in food spoilage. in the present work, a total of 102 strains of spoilage bacteria were isolated from pseudosciaena crocea at 4 °c storage, and of these, 60 strains were identified as shewanella spp., and 48 strains (47.1%) were identified as s. baltica. in addition, the spoilage capabilities of three different s. baltica strains ... | 2013 | 24206027 |
| the expression signatures of neuronal nitric oxide synthase (nos1) in turbot (scophthalmus maximus l.) mucosal surfaces against bacterial challenge. | the mucosal surfaces constitute the first immune barrier of host defense and also serve as the dynamic interfaces that simultaneously mediate a diverse array of critical physiological processes. it has been long hypothesized that observed difference of disease resistance among different fish strains and species are strongly correlated to the activities of the immune actors in mucosal surfaces. particularly, neuronal nos (nnos or nos1) is a constitutively expressed gene that catalyzes the oxidati ... | 2016 | 27825948 |
| the cooperative expression of heat shock protein 70 kd and 90 kd gene in juvenile larimichthys crocea under vibrio alginolyticus stress. | heat shock proteins (hsps) play significant roles in the immune response of fish in defending against diverse environmental threats or stresses. in this study, two complete hsp70 and hsp90 genes of larimichthys crocea (designated as lychsp70 and lychsp90) were identified and characterized (genbank accession no. kt456551 and kt456552). the complete open reading frame (orf) fragments of lychsp70 and lychsp90 were 1917 bp and 2151 bp, encoding 638 and 716 amino acids residues respectively. many sig ... | 2016 | 27678510 |
| characterisation and expression analysis of two terminal complement components: c7 and c9 from large yellow croaker, larimichthys crocea. | the large yellow croaker larimichthys crocea, as one of the most economically important marine fish in china and east asian countries, are facing the fatal attraction of various pathogens in recent years. elucidation of the organism immunomodulatory mechanism of croaker response to pathogen infection is essential for the disease control. in present study, we reported for the first time the molecular characterization and expression analysis of two terminal complement components (tccs) of croaker, ... | 2016 | 26902705 |
| abundant members of scavenger receptors family and their identification, characterization and expression against vibrio alginolyticus infection in juvenile larimichthys crocea. | scavenger receptors (srs) are crucial pattern recognition receptors (prrs) to defense pathogen infection in fish innate immunity. in this paper, some members in srs family of larimichthys crocea were identified, including eight genes in the class a, b, d and f families. (g + c) % of all srs members held 51% ∼ 59%, and these genes were no obvious codon bias by analyzing the distribution of a-, t-, g- and c-ended codons. the order of enc for all srs members by sequencing was lyccd68 > lycscara5 > ... | 2016 | 26876357 |
| the srec-i and srec-ii associated with epidermal growth factor in scavenger receptor family are the potential regulative transmembrane receptors in larimichthys crocea. | in innate immunity, the regulation of the immunologic gene expression plays a vital role in defense against pathogenic threat. the class f scavenger receptors (scarfs), a kind of crucial immunologic type i transmembrane receptors, mainly involve in the signal transmission and eliminating pathogens in host immune system. in this study, the srec-i and srec-ii of scarfs in larimichthys crocea (designated as lycsrec1 and lycsrec2 respectively) were first identified, the potential genetic locus relat ... | 2015 | 26343178 |
| molecular characterization of an il-1β gene from the large yellow croaker (larimichthys crocea) and its effect on fish defense against vibrio alginolyticus infection. | interleukin 1β (il-1β), the first interleukin to be characterized, plays a key role in regulating the immune response. in this study, we determined the cdna and genomic dna sequences of the il-1β gene from the large yellow croaker, larimichthys crocea. phylogenetic analysis indicated that the il-1β (lcil-1β) gene was most closely related to that of european seabass (dicentrarchus labrax), sharing 67.8% amino acid identity. in healthy large yellow croaker, lcil-1β transcription was detected in al ... | 2015 | 26018856 |
| identification and analysis of iccu/zn-sod, mn-sod and eccu/zn-sod in superoxide dismutase multigene family of pseudosciaena crocea. | superoxide dismutases (sods) belong to a significant and ubiquitous family of metalloenzymes for eliminating excess reactive oxygen species (ros). in this paper, the complete open reading frames (orfs) of intracellular cu/zn-sod (iccu/zn-sod), mn-sod and extracellular cu/zn-sod (eccu/zn-sod) were identified from the large yellow croaker (pseudosciaena crocea, designated as lycsod1, lycsod2 and lycsod3). the sequences were 465 bp, 678 bp and 645 bp (genbank accession no. kj908287, kj908285 and kj ... | 2015 | 25652289 |
| identification of scara3, scara5 and marco of class a scavenger receptor-like family in pseudosciaena crocea. | the class a scavenger receptors are important pattern recognition receptors of the innate immune system in living organisms. according to the whole-genome data of large yellow croaker (pseudosciaena crocea), three kinds of scavenger receptors, scara3, scara5 and marco were cloned from the spleen, designated severally as tycsa3, tycsa5 and tycmac. the complete cdnas open reading frames (orf) of tycsa3, tycsa5 and tycmac were 1938 bp, 1677 bp and 1218 bp (genbank accession no. kj467772, kj467773 a ... | 2014 | 25218683 |
| molecular characterization and functional analysis of two distinct liver-expressed antimicrobial peptide 2 (leap-2) genes in large yellow croaker (larimichthys crocea). | liver-expressed antimicrobial peptide 2 (leap-2) plays a vital role in the host innate immune system. in the present study, two leap-2 genes (lcleap-2a and lcleap-2c) from large yellow croaker (larimichthys crocea) were cloned, both of which consist of 3 exons and 2 introns. the lcleap-2a transcripts were expressed in a wide range of tissues, with the highest mrna levels found in the liver and intestine, while lcleap-2c transcripts showed obvious lower mrna levels in all tested tissues compared ... | 2014 | 24727197 |
| anti-infective mannose receptor immune mechanism in large yellow croaker (larimichthys crocea). | mannose receptor (mr) is a pattern recognition receptor (prr) that plays a significant role in immunity responses. its role has been described extensively in mammals, but very rarely in fish. recently, with the rapid development of an aquaculture industry cultivating large yellow croaker (larimichthys crocea), infectious diseases caused by viruses, bacteria and parasites are becoming more frequent and more severe, in particular bacterial infections caused by vibrio anguillarum, resulting in grea ... | 2016 | 27071518 |
| characterization, genomic organization, and expression profiles of myd88, a key adaptor molecule in the tlr signaling pathways in miiuy croaker (miichthys miiuy). | myeloid differentiation factor 88 (myd88) is an important adaptor protein in the tlr signaling pathways. in the present study, we firstly cloned and characterized miichthys miiuy myd88 (mimi-myd88) cdna and gene. the mimi-myd88 gene was 3,470 bp consisting of five exons and four introns. the cdna was composed of 1,627 bp with an 867-bp open reading frame encoding a polypeptide of 288 amino acid residues. the theoretical molecular mass and isoelectric point of this polypeptide were 33.25 and 4.96 ... | 2012 | 23053604 |
| molecular characterization and expression analysis of interferon-gamma in the large yellow croaker larimichthys crocea. | the large yellow croaker larimichthys crocea is an important mariculture fish species in china, and the bacterium vibrio harveyi (v. harveyi) and the ciliate protozoan cryptocaryon irritans (c. irritans) are the two major pathogens in its aquaculture sector. interferon-gamma (ifn-γ) plays important roles in regulating both innate and cell mediated immune responses as an inflammatory cytokine. in this study, we obtained the nucleotide sequence of ifn-γ from the large yellow croaker (lcifn-γ). the ... | 2015 | 26193669 |
| isolation and identification of the immune-relevant ribosomal protein l10 (rpl10/qm-like gene) from the large yellow croaker pseudosciaena crocea (pisces: sciaenidae). | in order to investigate the immune role of ribosomal protein l10 (rpl10/qm-like gene) in marine fish, we challenged the large yellow croaker pseudosciaena (= larimichthys) crocea, the most important marine fish culture species in china, by injection with a mixture of the bacteria vibrio harveyi and v. parahaemolyticus (3:1 in volume). microarray analysis and real-time pcr were performed 24 and 48 h post-challenge to isolate and identify the qm-like gene from the gill p. crocea (designated pcqm). ... | 2012 | 23096695 |
| expression profiles and interaction suggest tbk1 can be regulated by nrdp1 in response to immune stimulation in large yellow croaker larimichthys crocea. | tbk1 has been extensively studied in mammals because of its important roles as a molecular bridge, linking the tlrs (tlr3 and tlr4) and rlrs signals to activate transcriptional factors irf3 and irf7 for ifn-i production. however, the information on molecular and functional characteristics of tbk1 in teleosts is limited. in this study, the molecular characterization and immune response of tbk1 in larimichthys crocea (named as lctbk1) as well as its interaction with nrdp1 were investigated. sequen ... | 2015 | 26291490 |
| characterization and expression analysis of bca2 gene in large yellow croaker, larimichthys crocea. | bca2, as an e3 ubiquitin ligase, is an important anti-virus immune factor in mammals. up to date, there are not any related reports on bca2 protein in fishes yet. in the present investigation, bca2 in large yellow croaker larimichthys crocea (named as lcbca2) was identified and characterized. the full-length cdna of lcbca2 was 1571 bp, including an orf of 888 bp encoding a polypeptide of 295 amino acids. the putative lcbca2 protein contained a ring-h2 motif at c terminal. the lcbca2 transcripts ... | 2015 | 26208753 |
| characterization and expression analysis of toll-like receptor 2 gene in large yellow croaker, larimichthys crocea. | toll-like receptor 2 (tlr2) plays an important role in innate immune responses. here we describe the isolation and characterization of the full-length cdna sequence of toll-like receptor 2 in large yellow croaker larimichthys crocea (lctlr2). the lctlr2 cdna contains a 5'-terminal untranslated region (5'-utr) of 135 bp, an open reading frame (orf) of 2478 bp encoding a polypeptide of 825 amino acid residues and a 3'-utr of 50 bp. subcellular localization analysis suggested that the lctlr2-pegfp ... | 2015 | 25687392 |
| cloning and expression analysis of interferon regulatory factor (irf) 3 and 7 in large yellow croaker, larimichthys crocea. | the interferon regulatory factor (irf)3 and irf7 are considered to play essential roles in innate immune system's antiviral responses. in this report, the full-length cdna and genomic structure and immune response characterizations of irf3 and irf7 were investigated in large yellow croaker, larimichthys crocea. the full-length cdna of l. crocea (lc)irf3 was of 2204 bp, including a 5'-terminal untranslated region (utr) of 41 bp, a 3'-terminal utr of 774 bp and an open reading frame (orf) of 1389 ... | 2012 | 22374413 |
| research advances on animal genetics in china in 2015. | chinese scientists have made significant achievements in the field of animal genetics in 2015. incomplete statistics show that among all the publications of 2015 involving nematode (caenorhabditis elegans), fly (drosophila melanogaster), zebrafish (danio rerio), african clawed frog (xenopus) or mice (mus musculus), about 1/5 publications are from china. many innovative studies were published in high-impact international academic journals by chinese scientists, including the identification of a p ... | 2016 | 27655312 |
| establishment and characterization of a head kidney cell line from large yellow croaker pseudosciaena crocea. | a continuous cell line, lyck, derived from the head kidney of large yellow croaker pseudosciaena crocea was established and characterized. the lyck cell line multiplied well in leibovitz's-15 (l-15) medium supplemented with 10% foetal bovine serum (fbs) at 28° c. this cell line, with a population doubling time of 29·5 h at passage 35, has been subcultured for >70 passages. microscopically, lyck cells were fibroblast-like. chromosomal analysis revealed that the modal diploid chromosome number of ... | 2014 | 24773544 |
| molecular and functional characterization of toll-like receptor 21 in large yellow croaker (larimichthys crocea). | toll-like receptor 21 (tlr21) is a non-mammalian tlr that functions similar to mammalian tlr9 in recognizing cpg dna. in the present study, we identified a tlr21 homologue, lyctlr21, from large yellow croaker (larimichthys crocea). the complete coding sequence of lyctlr21 is 2946 nucleotides long, encoding a protein of 981 amino acids. the deduced lyctlr21 protein has typical tlr domain architecture, including a signal peptide, 13 leucine-rich repeats (lrrs) in the extracellular region, a transm ... | 2016 | 27751918 |
| structural characterization and functional analysis of the follistatin promoter of larimichthys crocea. | follistatin is a secreted glycoprotein, which involved in numerous physiological activities as an antagonist of the transforming growth factor-β superfamily. however, little is known about the regulation mechanism of follistatin in fish. in this study we cloned and analyzed part of the 5' flanking region of the follistatin gene in larimichthys crocea. sequence analysis revealed several putative binding sites for transcription factors including activator protein 1 (ap1), myogenic differentiation ... | 2016 | 27294388 |
| molecular characterization and expression analysis of tlr 7 and tlr 8 homologs in large yellow croaker (pseudosciaena crocea). | the two toll-like receptor (tlr) genes, lyctlr7 and lyctlr8, were cloned from large yellow croaker (pseudosciaena crocea), an economically important marine fish in china. the full-length cdnas of lyctlr7 and lyctlr8 are 3544 and 3593 bp, with an open reading frame (orf) of 3165 and 3093 bp, encoding 1053 and 1030 amino acids, respectively. the tlr family motifs, such as leucine rich repeat (lrr) and toll/interleukin (il)-1 receptor (tir) domain, are conserved in the lyctlr7 and lyctlr8, with 17 ... | 2013 | 23742866 |
| analysis of epa and dha in the viscera of marine fish using gas chromatography. | the viscera of 10 kinds of marine fishes were collected for fish oil extraction and detection of dha and epa, two most important polyunsaturated fatty acids. the fish oil extraction ratio for the evaluated fishes varied from 0.95% to 10.18% (wt%). pseudosciaena crocea presented the highest fish oil yield, followed by mustelus manazo, hippoglossus and sciaenopsocellatus. a gas chromatography method was then established for analysis of epa/dha. the epa concentration (in methyl ester form) in the f ... | 2016 | 27087076 |