complete sequence, analysis and organization of the orgyia leucostigma nucleopolyhedrovirus genome. | the complete genome of the orgyia leucostigma nucleopolyhedrovirus (orlenpv) isolated from the whitemarked tussock moth (orgyia leucostigma, lymantridae: lepidoptera) was sequenced, analyzed, and compared to other baculovirus genomes. the size of the orlenpv genome was 156,179 base pairs (bp) and had a g+c content of 39%. the genome encoded 135 putative open reading frames (orfs), which occupied 79% of the entire genome sequence. three inhibitor of apoptosis (orfs 16, 43 and 63), and five baculo ... | 2011 | 22163346 |
identification of a single-nucleocapsid baculovirus isolated from clanis bilineata tsingtauica (lepidoptera: sphingidae). | a nucleopolyhedrovirus isolated from infected larvae of clanis bilineata tsingtauica was characterized. electron microscopical studies on the ultrastructure of c. bilineata nucleopolyhedrovirus (clbisnpv) occlusion bodies (obs) showed several virions (up to 16) with a single nucleocapsid packaged within a single viral envelope. the diameter of the obs was 0.77-1.7 mum with a mean of 1.13 +/- 0.19 mum. the complete sequence of the clbisnpv polyhedrin (polh) gene contained 741 nucleotides, predict ... | 2008 | 18584114 |
genomic sequence, organization and characteristics of a new nucleopolyhedrovirus isolated from clanis bilineata larva. | baculoviruses are well known for their potential as biological agents for controlling agricultural and forest pests. they are also widely used as expression vectors in molecular cloning studies. the genome sequences of 48 baculoviruses are currently available in ncbi databases. as the number of sequenced viral genomes increases, it is important for the authors to present sufficiently detailed analyses and annotations to advance understanding of them. in this study, the complete genome of clanis ... | 2009 | 19243590 |
preparation of chitooligosaccharides from clanis bilineata larvae skin and their antibacterial activity. | clanis bilineata larvae are widely consumed in china. in this study, chitooligosaccharides were prepared from c. bilineata larvae skin by demineralisation, deproteination, washing, drying, deacetylation, hydrolysis using commercial α-amylase, filtration, setting the preparation at approximately 15% (w/v), precipitation with 6 volumes of ethanol, and drying at 60°c for 2 h. the optimal hydrolysis conditions were determined as follows: ph 5.5; temperature, 55°c; enzyme amount, 40 mg/(g chitosan); ... | 2012 | 22981818 |
hypolipidemic activity of the chitooligosaccharides from clanis bilineata (lepidoptera), an edible insect. | clanis bilineata (cb) is an edible insect, and cb larvae (cbl) are widely consumed in china, yielding a large number of cbl skin (cbls). in this study, chitooligosaccharides (cos) from cbls were prepared and their hypolipidemic activity was determined in rats. thirty-six male sprague-dawley rats in 3 groups were fed diets containing either high fat (hf) or cos, or a normal fat (nf) for 6 weeks. compared with hf group, cos group showed decreased body weight gain, plasma triacylglycerol (tg), tota ... | 2013 | 23591472 |
nutritional evaluation of protein from clanis bilineata (lepidoptera), an edible insect. | background: this study was carried out to determine the nutritive quality of protein from clanis bilineata (cb), an edible insect. results: the protein content of dried cb was 685.3 g kg(-1) and its essential amino acid content was 528.4 g kg(-1) , both of which are comparable to those of milk and eggs. three groups of rats were fed diets containing either casein or cb protein (cbp) as protein source or a protein-free diet for 10 days. the nutritional parameters measured for the group fed the d ... | 2011 | 22189909 |