| comamonas piscis sp. nov., isolated from the intestine of a korean rockfish, sebastes schlegelii. | a novel gram-stain-negative, obligately aerobic, non-motile and coccus-shaped bacterium, designated strain cn1t, was isolated from the intestine of a korean rockfish, sebastes schlegelii. the optimum growth condition for strain cn1t was 25 °c, at a ph of 7 and 0-1 % (w/v) nacl. the phylogenetic analysis based on the 16s rrna gene sequences showed that strain cn1t was in the genus comamonas and that the nearest type strain was comamonas koreensis yh11t with 98.65 % 16s rrna gene similarity. the p ... | 2015 | 26612039 |
| comprehensive characterization of three glutathione s-transferase family proteins from black rockfish (sebastes schlegelii). | glutathione s-transferases (gsts, ec 2.5.1.18) are categorized as phase ii enzymes, which form an important multifunctional family associated with a wide variety of catalytic activities. gstω, gstρ, and gstθ are cytosolic gsts which have been extensively studied in a variety of organisms; however, few studies have focused on teleosts. those paralogs from black rockfish (sebastes schlegelii; rfgstω, rfgstρ, and rfgstθ, respectively) were molecularly, biochemically, and functionally characterized ... | 2016 | 27449269 |
| comparative analysis of two thioredoxin-like genes in black rockfish sebastes schlegelii and their possible involvement in redox homeostasis and innate immune responses. | elevated levels of ros can cause serious intracellular damages by reacting readily with nucleic acids, proteins and lipids, thus triggering tissue damage and cell death. thioredoxin system is one of the principal factors that maintain the intracellular redox balance via its antioxidant property. in this study, we characterized two new thioredoxin isoforms (sstxn-like 1 and ssmttxn-like) from black rockfish, sebastes schlegelii. the molecular and structural characteristics, as well as the evoluti ... | 2017 | 27984102 |
| morphological and molecular characterization of pseudocohnilembus longisetus thompson, 1965 from farmed black rockfish sebastes schlegelii in korea. | the morphology, infraciliature, silverline system, and the small subunit ribosomal rna (ssu rrna) of the little-known marine scuticociliate pseudocohnilembus longisetusthompson, 1965 from the diseased black rockfish sebastes schlegelii in korea were studied. this scuticociliate possessed the typical characteristics of the genus pseudocohnilembus, but could be discriminated from pseudocohnilembus hargisi, and pseudocohnilembus persalinus in terms of the body size, shape, the number of somatic kin ... | 2011 | 21402450 |
| identification of a c-reactive protein like homologue from black rockfish (sebastes schlegelii) evidencing its potent anti-microbial properties at molecular level. | pentraxins are a family of evolutionary conserved proteins that contains two main members, namely c-reactive proteins (crps) and serum amyloid p (sap), which are involved in acute phase responses in animals. in this study, a cdna sequence of a crp-like molecule was identified from a previously constructed black rockfish cdna database (rfcrp) and subsequently characterized at its molecular level. the complete coding region of rfcrp is 672 bp in length, and encodes a protein containing 224 amino a ... | 2015 | 26182985 |
| a teleostan homolog of catalase from black rockfish (sebastes schlegelii): insights into functional roles in host antioxidant defense and expressional responses to septic conditions. | antioxidative defense renders a significant protection against environmental stress in organisms and maintains the correct redox balance in cells, thereby supporting proper immune function. catalase is an indispensable antioxidant in organisms that detoxifies hydrogen peroxides produced in cellular environments. in this study, we sought to molecularly characterize a homolog of catalase (rfcat), identified from black rockfish (sebastes schlegelii). rfcat consists of a 1581 bp coding region for a ... | 2015 | 25707597 |
| a c-type lectin that inhibits bacterial infection and facilitates viral invasion in black rockfish, sebastes schlegelii. | c-type lectins (ctls) are important pattern recognition receptors (prrs) that play vital roles in innate immunity. in teleosts, a number of ctls have been reported, but their in vivo effects on host defense are still limited. in this study, a ctl homolog (sslec1) was identified from black rockfish, sebastes schlegelii, and its structure, expression and biological function was analyzed. the open reading frame of sslec1 is 633 bp, with a 5'- untranslated region (utr) of 36 bp and a 3'- utr of 117 ... | 2016 | 27569982 |
| molecular characterization of a bactericidal permeability-increasing protein/lipopolysaccharide-binding protein from black rockfish (sebastes schlegelii): deciphering its putative antibacterial role. | bactericidal permeability-increasing protein (bpi)/lipopolysaccharide (lps) binding proteins (lbps) are well-known proteins that play an indispensable role in host antimicrobial defense. herein, we report a homolog of bpi/lbp from black rockfish (sebastes schlegelii) (designated as rfbpi/lbp) and characterize its structural and functional features at the molecular level. we identified the putative complete open reading frame (1422 bp) of rflbp that encodes a 474 amino acid protein with a predict ... | 2017 | 27663679 |
| identification and molecular characterization of two hepcidin genes from black rockfish (sebastes schlegelii). | antimicrobial peptides (amps) are important mediators of the immune response against bacteria and cysteine-rich peptide hepcidin is a 20-26 residues member with functions in iron regulation and antimicrobial activity. here, we have identified two different types of hepcidin cdna from the black rockfish, sebastes schlegelii, by est analysis. both hepcidin genes (hepcidin i and ii) consist of two introns and three exons that encode a prepropeptide (88 amino acids). a tata box and several consensus ... | 2008 | 18496731 |
| antibacterial action of l-amino acid oxidase from the skin mucus of rockfish sebastes schlegelii. | l-amino acid oxidase (lao) shows broadly antibacterial activity against gram-positive and gram-negative bacteria by h(2)o(2) generated in the oxidative process of l-amino acids. however, lao (termed ssap) isolated from the rockfish sebastes schlegelii skin mucus acted selectively on gram-negative bacteria. therefore, this study was undertaken to clarify the antibacterial action of ssap as compared with h(2)o(2). ssap inhibited potently the growth of aeromonas salmonicida, photobacterium damselae ... | 2008 | 18055243 |
| sequence variation in the genes encoding the major capsid protein, the atpase, and the rna polymerase 2 (domain 6) of lymphocystis disease virus isolated from schlegel's black rockfish, sebastes schlegelii hilgendorf. | | 2007 | 17640253 |
| stimulative effect of skipjack tuna soluble extract on pepsin-like protease in the stomach of rockfish (sebastes schlegelii) using an in vitro perfusion method. | the effect of intra-gastric infusion of skipjack tuna (katsuwonus pelamis) muscle soluble extract and of carnosine, anserine and histidine, on pepsin-like protease activity was studied in the isolated, externally batch-cultured stomach of the rockfish (sebastes schlegelii). stomach was isolated from the fish, then intragastrically infused with the above solutions or with a balanced saline solution (control solution) as the stimulant at 0.1 ml/min for 6h. intra-gastric efflux was collected for me ... | 2011 | 21130891 |
| molecular delineation of a caspase 10 homolog from black rockfish (sebastes schlegelii) and its transcriptional regulation in response to pathogenic stress. | caspase 10 is an initiator caspase in death cascades of death receptor mediated apoptotic signaling. we identified and molecularly characterized a novel homolog of caspase 10 from black rockfish (sebastes schlegelii) and designated as rfcasp10. the complete coding region of rfcasp10 was found to consist of 1659 bps, encoding a 553 amino acid protein with a predicted molecular mass of 61.7 kda. the characteristic caspase family domain architecture, including death effecter domains (deds), was cle ... | 2015 | 26048002 |
| a thioredoxin domain-containing protein 12 from black rockfish sebastes schlegelii: responses to immune challenges and protection from apoptosis against oxidative stress. | thioredoxin (txn) superfamily proteins are identified by the presence of a thioredoxin active site with a conserved cxxc active motif. txn members are involved in a wide range of biochemical and biological functions including redox regulation, refolding of disulfide containing proteins, and regulation of transcription factors. in the present study, a thioredoxin domain-containing protein 12 was identified and characterized from black rockfish, sebastes schlegelii (rftxndc12). the full length of ... | 2017 | 26945103 |
| identification of scuticociliates (pseudocohnilembus persalinus, p. longisetus, uronema marinum and miamiensis avidus) based on the cox1 sequence. | scuticociliatosis is characterized as highly histophagous, causing systemic tissue destruction and high mortality in cultured marine fish. some of the scuticociliates have been implicated as the causative agents of scuticociliatosis. here, we describe our study to differentially identify various species in complex animal-sourced samples, namely olive flounder paralichthys olivaceus and black rockfish sebastes schlegelii suffering from scuticociliatosis. the mitochondrial cytochrome c oxidase 1 ( ... | 2013 | 22951379 |
| algicidal activity of marine alteromonas sp. kns-16 and isolation of active compounds. | the kns-16 algicidal strain was isolated from a harmful alga bloom (hab) area and identified as alteromonas sp. based on 16s rdna sequencing. the kns-16 strain was found to control habs by producing algicidal compounds in an indirect interaction. four active compounds were isolated from kns-16 culture, and their structures were analyzed by interpreting nuclear magnetic resonance and mass spectroscopy data. the structures were identified as 2-undecen-1'-yl-4-quinolone (1), 2-undecyl-4-quinolone ( ... | 2012 | 22878186 |
| a high-mobility group box 1 that binds to dna, enhances pro-inflammatory activity, and acts as an anti-infection molecule in black rockfish, sebastes schlegelii. | high-mobility group box (hmgb) 1 is a chromosomal protein that plays critical roles in dna transcription, replication and repair. in addition, hmgb1 functions as a pro-inflammatory molecule in many vertebrates and invertebrates. in teleosts, very limited studies of hmgb1 have been reported. in this study, we identified a hmgb1 homologue (sshmgb1) from black rockfish (sebastes schlegelii) and analyzed its structure, expression and biological function. the open reading frame of sshmgb1 is 621 bp, ... | 2016 | 27492120 |
| simplicispira piscis sp. nov., isolated from the gut of a korean rockfish, sebastes schlegelii. | a novel gram-stain-negative, aerobic, motile and rod-shaped bacterium, designated strain rsg39t, was isolated from the gut of a korean rockfish, sebastes schlegelii. the 16s rrna gene sequence analysis revealed that strain rsg39t belonged to the genus simplicispira in the class betaproteobacteria and its highest sequence similarity was shared with s. psychrophila (98.4 %). the isolate grew optimally at 20 °c, at ph 7 and with 0 % (w/v) nacl. the main respiratory quinone of the isolate was ubiqui ... | 2015 | 26400548 |