| molecular characterization of allium virus x, a new potexvirus in the family alphaflexiviridae, infecting ornamental allium. | abtract: a new potexvirus affecting ornamental allium spp in the netherlands was identified and characterized at the molecular level. the virus had a single-stranded rna genome of 7100 bp (excluding the 18 bp poly a tail). the genome organization was found to be typical of members of the genus potexvirus and consisted of five open reading frames (orf). nucleotide and amino acid sequence comparisons with those of known potexvirus members showed that this virus is related to hosta virus x and hydr ... | 2011 | 21938462 |
| phylogeny, coat protein genetic variability, and transmission via seeds of hosta virus x. | the complete genome of hosta virus x (hvx), which is thought to be a distinct species of potexvirus, was sequenced. nucleotide sequences of hvx were compared with those of other members of the genus potexvirus and phylogenetic tree was constructed. the range of identities of viral replicase open reading frame 1 (orf1) between hvx and other potexviruses were 43.1%-55.1% and 35.9%-46.6% at the nucleotide and amino acid levels, respectively. phylogenetic analysis was performed according to the amin ... | 2012 | 22453807 |
| the variability of hosta virus x isolates in the czech republic. | the coat protein (cp) gene dna sequences of nine isolates of hosta virus x (hvx) from different regions of the czech republic were determined and compared with sequences available in genbank. the sequences were almost uniform, the pairwise nucleotide identities among the czech hvx isolates were 99-100%. the respective range was 98-100% when sequences from the genbank were included. therefore, phylogenetic analyses including maximum parsimony and bayesian analyses of either, dna and deduced amino ... | 2013 | 24020762 |
| biological and molecular characterization of a u.s. isolate of hosta virus x. | hosta virus x (hvx) is rapidly becoming a serious pathogen of commercially important hosta plants worldwide. we report here biological and molecular characterization of a u.s. isolate of hvx, hvx-37. hvx-37 infectivity was tested in 21 hosta cultivars over three growth seasons, and three types of responses were defined based upon the ability of the virus to cause local and/or systemic infections. four cultivars resistant to systemic hvx infection were identified. the full-length sequence of the ... | 2012 | 22809286 |
| genetic variability and phylogenetic analysis of hosta virus x. | triple gene block 1 (tgb1) and coat protein (cp) sequences of 30 hosta virus x (hvx) isolates from tennessee (tn), usa, were determined and compared with available sequences in genbank. the cps of all known hvx isolates, including those from tn, shared 98.3-100% and 98.2-100% nucleotide and amino acid sequence identity, respectively, whereas tgb1 shared 97.4-100% nucleotide and 97-100% amino acid sequence identity. tgb1 of tn isolates were all longer by one codon from that of a korean isolate, w ... | 2009 | 19838620 |
| molecular evidence supporting the classification of hosta virus x as a distinct species of the genus. | apotexvirus, hosta virus x (hvx-kr), causing mosaic and mottle symptoms was isolated from hosta plants ( hosta spp.) in korea. the 3'-terminal 2,711 nucleotides excluding the poly (a) tail were determined and shown to include the partial viral replicase, triple gene block (tgb) 1 (26 kda), tgb2 (13 kda), tgb3 (8 kda), and 23 kda coat protein (cp) and the 3'-nontranslated region (ntr), typical of potexviruses. the cp gene of the type isolate of hvx (hvx-u) was amplified by rt-pcr and its nucleoti ... | 2003 | 14551823 |