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perlucidibaca piscinae gen. nov., sp. nov., a freshwater bacterium belonging to the family moraxellaceae.a freshwater bacterium, designated imcc1704(t), was isolated from a eutrophic pond. the strain was gram-negative, oxidase-positive, catalase-negative, chemoheterotrophic and facultatively aerobic with cells that were motile rods with a single polar flagellum. based on 16s rrna gene sequence similarity analyses, the novel strain was most closely related to the genera alkanindiges (91.7%), acinetobacter (89.0-91.2%), moraxella (87.9-90.1%), psychrobacter (87.2-89.5%) and enhydrobacter (87.8%). phy ...200818175691
Acinetobacter Insertion Sequence ISAba11 Belongs to a Novel Family That Encodes Transposases with a Signature HHEK Motif.Experimental and in silico PCR analysis targeting ISAba11 and TnAbaR islands in 196 epidemiologically unrelated Acinetobacter strains representative of =19 species were performed. The first two Acinetobacter baumannii ISAba11 elements identified had been found to map to the same site on TnAbaR transposons. However, no further evidence of physical linkage between the two elements was demonstrated. Indeed, examination of 25 definite or putative insertion sites suggested limited sequence specificit ...201222081580
the genus enhydrobacter staley et al. 1987 should be recognized as a member of the family rhodospirillaceae within the class alphaproteobacteria.the genus enhydrobacter, firstly reported as a member of the family vibrionaceae, has been placed in the family moraxellaceae, but as a genus incertae sedis in bergey's manual of systematic bacteriology 2(nd) edition. during our taxonomic investigation of enhydrobacter like organisms, we observed that the 16s rrna sequences of e. aerosaccus type strain versions ncimb 12535(t) , atcc 27094( t) and ccug 58314(t) were very different from the accessible data (accession no. aj550856). phylogenetic ...201122145860
biodiversity in traditional polish cheese oscypek determined by culture-dependent and -independent approaches.oscypek is a traditional polish, scalded-smoked cheese, with a protected designation of origin (pdo) status manufactured from raw sheep's milk without starter cultures in the tatra mountains region of poland. this study was undertaken in order to get some insight on the microbiota which develops and evolves during the manufacture and ripening stages of oscypek. to this end, we made use of both culturing and the culture-independent methods of pcr-dgge and pyrosequencing of 16s rrna gene amplicons ...201222247135
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