| sulfurihydrogenibium subterraneum gen. nov., sp. nov., from a subsurface hot aquifer. | a polyphasic taxonomic study was performed on a novel facultatively anaerobic, hydrogen- or sulfur/thiosulfate-oxidizing, thermophilic chemolithoautotroph recently isolated from subsurface hot aquifer water in a japanese gold mine. the cells were straight to slightly curved rods, with a single polar flagellum. growth was observed at 40-70 degrees c (optimum 60-65 degrees c; 80 min doubling time) and at ph 6.4-8.8 (optimum ph 7.5). the isolate was unable to use complex organic compounds, carbohyd ... | 2003 | 12807207 |
| molecular characterization of community structures and sulfur metabolism within microbial streamers in japanese hot springs. | community structures of submerged microbial slime streamers (smss) in sulfide-containing hot springs at 72 to 80 degrees c at nakabusa and yumata, japan, were investigated by molecular analysis based on the 16s rrna gene. the smss were classified into two consortia; consortium i occurred at lower levels of sulfide in the hot springs (less than 0.1 mm), and consortium ii dominated when the sulfide levels were higher (more than 0.1 mm). the dominant cell morphotypes in consortium i were filamentou ... | 2003 | 14660348 |
| sulfurihydrogenibium azorense, sp. nov., a thermophilic hydrogen-oxidizing microaerophile from terrestrial hot springs in the azores. | five hydrogen-oxidizing, thermophilic, strictly chemolithoautotrophic, microaerophilic strains, with similar (99-100%) 16s rrna gene sequences were isolated from terrestrial hot springs at furnas, são miguel island, azores, portugal. the strain, designated az-fu1t, was characterized. the motile, 0.9-2.0 microm rods were gram-negative and non-sporulating. the temperature growth range was from 50 to 73 degrees c (optimum at 68 degrees c). the strains grew fastest in 0.1% (w/v) nacl and at ph 6, al ... | 2004 | 14742456 |
| polyamine analysis for chemotaxonomy of thermophilic eubacteria: polyamine distribution profiles within the orders aquificales, thermotogales, thermodesulfobacteriales, thermales, thermoanaerobacteriales, clostridiales and bacillales. | cellular polyamines of 45 thermophilic and 8 related mesophilic eubacteria were investigated by hplc and gc analyses for the thermophilic and chemotaxonomic significance of polyamine distribution profiles. spermidine and a quaternary branched penta-amine, n4-bis(aminopropyl)norspermidine, were the major polyamine in thermocrinis, hydrogenobacter, hydrogenobaculum, aquifex, persephonella, sulfurihydrogenibium, hydrogenothermus, balnearium and thermovibrio, located in the order aquificales. thermo ... | 2004 | 15747232 |
| bacterial community shift along a subsurface geothermal water stream in a japanese gold mine. | change of bacterial community occurring along a hot water stream in the hishikari gold mine, japan, was investigated by applying a combination of various culture-independent techniques. the stream, which is derived from a subsurface anaerobic aquifer containing plentiful co2, ch4, h2, and nh4+, emerges in a mine tunnel 320 m below the surface providing nutrients for a lush microbial community that extends to a distance of approximately 7 m in the absence of sunlight-irradiation. over this distan ... | 2005 | 15776216 |
| sulfurihydrogenibium yellowstonense sp. nov., an extremely thermophilic, facultatively heterotrophic, sulfur-oxidizing bacterium from yellowstone national park, and emended descriptions of the genus sulfurihydrogenibium, sulfurihydrogenibium subterraneum and sulfurihydrogenibium azorense. | a novel thermophilic, sulfur-oxidizing gram-negative bacterium, designated strain ss-5t, was isolated from the calcite hot springs in yellowstone national park, usa. the cells were motile rods (1.2-2.8 microm long and 0.6-0.8 microm wide). the new isolate was a facultative heterotroph capable of using elemental sulfur or thiosulfate as an electron donor and o2 (1-18 %; optimum 6 %, v/v) as an electron acceptor. hydrogen did not support growth. the isolate grew autotrophically with co2. in additi ... | 2005 | 16280480 |
| molecular signatures in protein sequences that are characteristics of the phylum aquificae. | species of the phylum aquificae are of great interest due to their strict extreme thermophilic growth characteristics. presently, there is no known molecular characteristic which is unique to this group of bacteria. this work describes six conserved inserts and deletions (indels or signature sequences) in four widely distributed proteins that are distinctive features of species from the phylum aquificae. these include three signatures consisting of a 2 aa insert, a 5-6 aa insert and a 6 aa delet ... | 2006 | 16403873 |
| thermostable rnase p rnas lacking p18 identified in the aquificales. | the rnase p rna (rnpb) and protein (rnpa) genes were identified in the two aquificales sulfurihydrogenibium azorense and persephonella marina. in contrast, neither of the two genes has been found in the sequenced genome of their close relative, aquifex aeolicus. as in most bacteria, the rnpa genes of s. azorense and p. marina are preceded by the rpmh gene coding for ribosomal protein l34. this genetic region, including several genes up- and downstream of rpmh, is uniquely conserved among all thr ... | 2006 | 17005927 |
| microbial life in champagne pool, a geothermal spring in waiotapu, new zealand. | surveys of champagne pool, one of new zealand's largest terrestrial hot springs and rich in arsenic ions and compounds, have been restricted to geological and geochemical descriptions, and a few microbiological studies applying culture-independent methods. in the current investigation, a combination of culture and culture-independent approaches were chosen to determine microbial density and diversity in champagne pool. recovered total dna and adenosine 5'-triphosphate (atp) content of spring wat ... | 2007 | 17426919 |
| venenivibrio stagnispumantis gen. nov., sp. nov., a thermophilic hydrogen-oxidizing bacterium isolated from champagne pool, waiotapu, new zealand. | a novel thermophilic, hydrogen-oxidizing bacterium, designated strain cp.b2(t), was isolated from a terrestrial hot spring in waiotapu, new zealand. cells were motile, slightly rod-shaped, non-spore-forming and gram-negative. isolate cp.b2(t) was an obligate chemolithotroph, growing by utilizing h(2) as electron donor and o(2) as corresponding electron acceptor. elemental sulfur (s(0)) or thiosulfate ( ) was essential for growth. microbial growth occurred under microaerophilic conditions in 1.0- ... | 2008 | 18218938 |
| ecophysiology and geochemistry of microbial arsenic oxidation within a high arsenic, circumneutral hot spring system of the alvord desert. | microbial metabolism of arsenic has gained considerable interest, due to the potential of microorganisms to drive arsenic cycling and significantly influence the geochemistry of naturally arsenic-rich or anthropogenically arsenic-polluted environments. alvord hot spring in southeastern oregon is a circumneutral hot spring with an average arsenic concentration of 4.5 mg l(-1) (60 microm). hydrogeochemical analyses indicated significant arsenite oxidation, increased ph and decreased temperature al ... | 2008 | 18318711 |
| volcanic calderas delineate biogeographic provinces among yellowstone thermophiles. | it has been suggested that the distribution of microorganisms should be cosmopolitan because of their enormous capacity for dispersal. however, recent studies have revealed that geographically isolated microbial populations do exist. geographic distance as a barrier to dispersal is most often invoked to explain these distributions. here we show that unique and diverse sequences of the bacterial genus sulfurihydrogenibium exist in yellowstone thermal springs, indicating that these sites are geogr ... | 2008 | 18363714 |
| sulfurihydrogenibium rodmanii sp. nov., a sulfur-oxidizing chemolithoautotroph from the uzon caldera, kamchatka peninsula, russia, and emended description of the genus sulfurihydrogenibium. | four thermophilic, sulfur-oxidizing, chemolithoautotrophic strains with >99 % 16s rrna gene sequence similarity were isolated from terrestrial hot springs in the geyser valley and the uzon caldera, kamchatka, russia. one strain, designated uz3-5t, was characterized fully. cells of uz3-5t were gram-negative, motile, slightly oval rods (about 0.7 microm wide and 1.0 microm long) with multiple polar flagella. all four strains were obligately microaerophilic chemolithoautotrophs and could use elemen ... | 2008 | 18450704 |
| sulfurihydrogenibium kristjanssonii sp. nov., a hydrogen- and sulfur-oxidizing thermophile isolated from a terrestrial icelandic hot spring. | three thermophilic, aerobic, hydrogen- and sulfur-oxidizing bacteria were isolated from an icelandic hot spring near the town of hveragerdi and share >99 % 16s rrna gene sequence similarity. one of these isolates, designated strain i6628t, was selected for further characterization. strain i6628t is a motile rod, 1.5-2.5 microm long and about 0.5 microm wide. growth occurred between 40 and 73 degrees c (optimally at 68 degrees c), at ph 5.3-7.8 (optimally at ph 6.6) and at nacl concentrations bet ... | 2008 | 18450705 |
| molecular characterization of the diversity and distribution of a thermal spring microbial community by using rrna and metabolic genes. | the diversity and distribution of a bacterial community from coffee pots hot spring, a thermal spring in yellowstone national park with a temperature range of 39.3 to 74.1 degrees c and ph range of 5.75 to 6.91, were investigated by sequencing cloned pcr products and quantitative pcr (qpcr) of 16s rrna and metabolic genes. the spring was inhabited by three aquificae genera--thermocrinis, hydrogenobaculum, and sulfurihydrogenibium--and members of the alpha-, beta-, and gammaproteobacteria, firmic ... | 2008 | 18539788 |
| complete and draft genome sequences of six members of the aquificales. | the aquificales are widespread in marine and terrestrial hydrothermal environments. here, we report the complete and draft genome sequences of six new members of the aquificales: two marine species, persephonella marina strain ex-h1 and hydrogenivirga strain 128-5-r1 (from the east pacific rise, 9 degrees 50.3'n, 104 degrees 17.5'w, and the eastern lau spreading center, 176 degrees 11.5'w, 20 degrees 45.8's, respectively), and four terrestrial isolates, sulfurihydrogenibium azorense strain az-fu ... | 2009 | 19136599 |
| linking microbial oxidation of arsenic with detection and phylogenetic analysis of arsenite oxidase genes in diverse geothermal environments. | the identification and characterization of genes involved in the microbial oxidation of arsenite will contribute to our understanding of factors controlling as cycling in natural systems. towards this goal, we recently characterized the widespread occurrence of aerobic arsenite oxidase genes (aroa-like) from pure-culture bacterial isolates, soils, sediments and geothermal mats, but were unable to detect these genes in all geothermal systems where we have observed microbial arsenite oxidation. co ... | 2009 | 19196273 |
| sulfur-metabolizing bacterial populations in microbial mats of the nakabusa hot spring, japan. | at the nakabusa hot spring, japan, dense olive-green microbial mats develop in regions where the slightly alkaline, sulfidic effluent has cooled to 65°c. the microbial community of such mats was analyzed by focusing on the diversity, as well as the in situ distribution and function of bacteria involved in sulfur cycling. analyses of 16s rrna and functional genes (apra, pufm) suggested the importance of three thermophilic bacterial groups: aerobic chemolithotrophic sulfide-oxidizing species of th ... | 2011 | 21353426 |
| microbial communities and chemosynthesis in yellowstone lake sublacustrine hydrothermal vent waters. | five sublacustrine thermal spring locations from 1 to 109ôçëm water depth in yellowstone lake were surveyed by 16s ribosomal rna gene sequencing in relation to their chemical composition and dark co(2) fixation rates. they harbor distinct chemosynthetic bacterial communities, depending on temperature (16-110-¦c) and electron donor supply (h(2)s <1 to >100ôçë++m; nh(3) <0.5 to >10ôçë++m). members of the aquificales, most closely affiliated with the genus sulfurihydrogenibium, are the most frequen ... | 2011 | 21716640 |