| molecular characterization of a distinct begomovirus infecting tobacco in yunnan, china. | virus isolate y3v, obtained from tobacco showing leaf curl symptoms in yunnan, china, had particles with the size and morphology typical of geminiviruses. in reactions with a set of monoclonal antibodies raised against begomoviruses, y3v was readily differentiated from two previously studied chinese begomovirus isolates. the complete nucleotide sequence of a dna-a-like molecule of y3v was determined; it comprises 2744 nucleotides and has a typical begomovirus genome organization. when compared w ... | 2001 | 11676420 |
| molecular characterization of tomato-infecting begomoviruses in yunnan, china. | the importance of diseases of tomato caused by begomoviruses is increasing worldwide. here, we report that several begomoviruses are associated with tomato leaf curl disease in yunnan province, china. 14 tomato samples showing leaf curl symptoms were collected in three districts in yunnan, and they fell into four groups according to their reaction with a panel of 16 monoclonal antibodies in tas-elisa. comparison of partial dna-a sequences amplified with degenerate primers confirmed the existence ... | 2004 | 15593415 |
| molecular characterization of a new begomovirus infecting sida cordifolia and its associated satellite dna molecules. | two virus isolates hn57 and hn60 were obtained from sida cordifolia showing mild upward leaf-curling symptoms in hainan province of china. comparison of partial sequences of dna-a like molecule confirmed the existence of a single type of begomovirus. the complete nucleotide sequence of dna-a of hn57 was determined to be 2757 nucleotides, with a genomic organization typical of begomoviruses. complete sequence comparison with other reported begomoviruses revealed that hn57 dna-a has the highest se ... | 2006 | 16990998 |
| a naturally occurring defective dna satellite associated with a monopartite begomovirus: evidence for recombination between alphasatellite and betasatellite. | monopartite begomoviruses and their associated satellites form unique disease complexes that have emerged as a serious threat to agriculture worldwide. it is well known that frequent recombination contributes to the diversification and evolution of geminiviruses. in this study, we identified a novel defective satellite molecule (recsat) in association with tobacco leaf curl yunnan virus (tblcynv) in a naturally infected tobacco plant. sequence analysis showed that recsat comprises 754 nucleotide ... | 2013 | 24018984 |
| development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against tomato yellow leaf curl thailand virus. | tomato yellow leaf curl thailand virus, tylcthv, is a begomovirus that causes severe losses of tomato crops in thailand as well as several countries in southeast and east asia. the development of monoclonal antibodies (mabs) and serological methods for detecting tylcthv is essential for epidemiological studies and screening for virus-resistant cultivars. | 2017 | 28558726 |