| use of random amplified polymorphic dna analysis and single-enzyme amplified fragment length polymorphism in molecular typing of ornithobacterium rhinotracheale strains. | ornithobacterium rhinotracheale (ort) is a bacterium common to commercial poultry and wild birds throughout the world. it is also known as a causative agent of respiratory diseases. a total of 93 ort isolates originating from chickens, pigeons, ostriches, quail, turkeys, and an asian crested goshawk (accipiter trivirgatus) in taiwan, between 2004 and 2006, were used in this study. high genetic similarity (97%-100%) in 16s rrna sequence was revealed among the 50 randomly selected isolates, in add ... | 2009 | 19432012 |
| frequent detection of anticoagulant rodenticides in raptors sampled in taiwan reflects government rodent control policy. | anticoagulant rodenticides (ars) are known to cause extensive secondary exposure in top predators in europe and north america, but there remains a paucity of data in asia. in this study, we collected 221 liver samples from 21 raptor species in taiwan between 2010 and 2018. most birds were recovered from rescue organizations, but some free-ranging individuals were obtained from bird-strike prevention measures at airports. ars were detected in 10 species and more than half of the total samples. co ... | 2019 | 31326797 |
| validation of spilornis cheela hoya taqman probes for potential gender identification of many accipitridae species. | the objective of this study was to test the hypothesis that genders of accipitridae species, with the same or similar sequences to our previously proposed spilornis cheela hoya (s. c. hoya) chromo-helicase-dna binding protein (chd)-w-specific and chd-zw-common taqman probes, can be successfully determined. eight species of accipitridae with known genders were collected. after pcr, ta cloning, sequencing, and alignment analyses, sequence length differences of griffiths p2/p8 pcr amplicons between ... | 2010 | 19959218 |
| use of random amplified polymorphic dna to identify several novel markers for sex identification in the crested serpent eagle and crested goshawk. | the crested serpent eagle (spilornis cheela hoya) has no distinct sexual dimorphic traits. in the current study, we report the results of an ee0.6 (ecori 0.6-kb fragment) sequence applied to s. cheela hoya and a novel random amplified polymorphic dna (rapd) marker that can be used to sex individuals within the species s. cheela hoya and accipiter trivigatus formosae (crested goshawk). we used sex-specific primers for the avian chd1 (chromo-helicase-dna-binding 1) gene and the ee0.6 sequence in p ... | 2009 | 19560806 |