| [epidemiological observations on the first case of human paragonimiasis and potential intermediate hosts of paragonimus sp. in benin]. | parasitological investigations were carried out for four months in 1998 in two beninese centres of pneumo-phtisiology (akpakpa, at cotonou, and akron, at porto-novo) to detect the patients harbouring eggs of paragonimus sp. amongst the persons consulting for tuberculosis and showing a broncho-pneumopathy without mycobacteria. eggs of paragonimus sp. were detected in the sputum of a single patient out of 369 persons examined (prevalence, 0.2%). this patient had eaten crabs in the months preceding ... | 1999 | 10472447 |
| co-infection by a yeast-like organism in hematodinium-infected european edible crabs cancer pagurus and velvet swimming crabs necora puber from the english channel. | during the winter months, edible crabs cancer pagurus and velvet swimming crabs necora puber from the english channel can harbour infections by a hematodinium sp. dinoflagellate. this parasite is responsible for a highly pathological condition known as 'pink crab disease' (pcd) in the edible crab. in the current study, a high proportion (between 25 and 100%) of hematodinium-infected edible and velvet swimming crabs captured from 2 sites in the english channel also harboured a systemic infection ... | 2003 | 12803383 |
| bacteriology of unshelled frozen blue swimming crab (portunus pelagicus). | in this study, 45 (10 whole specimens and 35 frozen claws) frozen samples of portunus pelagicus imported into sicily (italy) from the west coast of africa were examined to assess their bacteriological characteristics and suitability for consumption. bacteriological examination was performed on two subsamples for each whole crab. the first was the body and claw muscle; the second was a pool of viscera and gills. in the case of frozen claws, each muscle claw was a sample. an aerobic plate count at ... | 2004 | 15083736 |
| phylogenetic relationships of 12 penaeoidea shrimp species deduced from mitochondrial dna sequences. | dna sequences of an 847 bp fragment of mitochondrial cytochrome oxidase subunit i (coi) gene and a 514 bp fragment of 16s rrna gene were determined to examine the phylogenetic relationships of 12 penaeoidea shrimp species (penaeus chinensis, penaeus japonicus, penaeus penicillatus, penaeus vannamei, penaeus canaliculatus, trachypenaeus curvirostris, metapenaeus affinis, metapenaeus ensis, metapenaeopsis barbata, parapenaeus fissuroides, parapenaeopsis hardiwickii, solenocera crassicomis). both f ... | 2004 | 15524311 |
| the complete mitochondrial genome of the stomatopod crustacean squilla mantis. | animal mitochondrial genomes are physically separate from the much larger nuclear genomes and have proven useful both for phylogenetic studies and for understanding genome evolution. within the phylum arthropoda the subphylum crustacea includes over 50,000 named species with immense variation in body plans and habitats, yet only 23 complete mitochondrial genomes are available from this subphylum. | 2005 | 16091132 |
| a review of the parasitic dinoflagellates hematodinium species and hematodinium-like infections in marine crustaceans. | parasitic dinoflagellates in the genus hematodinium are important parasites of marine crustacea. outbreaks of these parasites have damaged commercial stocks of norway lobster nephrops norvegicus, snow crab chionoecetes opilio, tanner crab c. bairdi, american blue crab callinectes sapidus, and velvet swimming crab necora puber. species of hematodinium can reach high enough levels to regulate their host populations, but mortalities are also centred on the unfished juveniles and females, hosts not ... | 2005 | 16175968 |
| stone-tool usage by thai long-tailed macaques (macaca fascicularis). | in january and march of 2005, we conducted surveys of long-tailed macaques at piak nam yai island, laem son national park (9 degrees n 34-35', 98 degrees e 28'), ranong province, situated in southern thailand. two of the three troops of long-tailed macaques found on the island were observed using axe-shaped stones to crack rock oysters, detached gastropods (thais tissoti, petit, 1852), bivalves (gafrarium divaricatum, gmelin, 1791), and swimming crabs (thalamita danae, stimpson, 1858). they smas ... | 2007 | 17146796 |
| a marine killer yeast against the pathogenic yeast strain in crab (portunus trituberculatus) and an optimization of the toxin production. | a pathogenic yeast strain wcy which could cause milky disease in portunus trituberculatus was identified to be metschnikowia bicuspidate according to the results of routine yeast identification and 18s rdna and its sequences. after screening of more than 300 yeast strains from different sources in marine environments, it was found that strain yf07b had the highest ability to produce killer toxin against the pathogenic yeast. strain yf07b was identified to be pichia anomala according to the resul ... | 2007 | 17188476 |
| organochlorinated contaminants in decapod crustaceans from the coasts of brittany and normandy (france). | the contamination and distribution of organochlorinated compounds were considered in three crustacean species (edible crab, cancer pagurus; spider crab, maja brachydactyla; velvet swimming crab, necora puber) from five sites along the coasts of brittany and normandy (western and north-western france). pcbs (16 single congeners), pp'-dde and hcb were measured in hepatopancreas, gonads and muscle: in all, 175 samples were analysed. the spider crab was the only species found in the five sampling si ... | 2007 | 17223177 |
| genetic heterogeneity of the blue swimming crab (portunus pelagicus) in thailand determined by aflp analysis. | genetic diversity and population differentiation of the blue swimming crab (portunus pelagicus) in thailand, originating from ranong and krabi located in the andaman sea (west) and chanthaburi, prachuap khiri khan, and suratthani located in the gulf of thailand (east), were examined by aflp analysis. high genetic diversity of p. pelagicus in thai waters was found (n=72). the four primer combinations generated 227 aflp fragments, and the percentage of polymorphic bands in each geographic sample w ... | 2007 | 17879155 |
| development of a species-diagnostic scar marker of the blue swimming crab (portunus pelagicus). | | 2007 | 18000751 |
| a c-type lectin like-domain (ctld)-containing protein (ptlp) from the swimming crab portunus trituberculatus. | c-type lectins play important roles in the non-self innate immune system of invertebrates. in this study, we isolated the full-length cdna of the c-type lectin like-domain (ctld)-containing protein, designated ptlp, from the hepatopancreas of the swimming crab portunus trituberculatus, one of the most common edible crabs of east asia. the ptlp cdna consists of 923 bp and encodes a polypeptide of 164 amino acids containing a well-conserved c-type lectin like-domain (ctld). the deduced amino acid ... | 2008 | 18603446 |
| marine killer yeasts active against a yeast strain pathogenic to crab portunus trituberculatus. | some marine yeasts have recently been recognised as pathogenic agents in crab mariculture, but may be inhibited or killed by 'killer' yeast strains. we screened multiple yeast strains from seawater, sediments, mud of salterns, guts of marine fish, and marine algae for killer activity against the yeast metchnikowia bicuspidata wcy (pathogenic to crab portunus trituberculatus), and found 17 strains which could secrete toxin onto the medium and kill the pathogenic yeast. of these, 5 strains had sig ... | 2008 | 18814546 |
| hemolymph ionic regulation and adjustments in gill (na+, k+)-atpase activity during salinity acclimation in the swimming crab callinectes ornatus (decapoda, brachyura). | we evaluate hemolymph osmotic and ionic regulatory abilities and characterize a posterior gill microsomal (na+, k+)-atpase from the marine swimming crab, callinectes ornatus, acclimated to 21 per thousand or 33 per thousand salinity. c. ornatus is isosmotic after acclimation to 21 per thousand but is hyposmotic at 33 per thousand salinity; hemolymph ions do not recover initial levels on acclimation to 21 per thousand salinity but are anisoionic compared to ambient concentrations, revealing modes ... | 2009 | 19422928 |
| here and there: a preliminary note on the prevalence of an alien rhizocephalan parasite at the southern and northern limits of its introduced range. | the erythrean invasive swimming crab charybdis longicollis established dense populations in the levantine basin of the mediterranean sea in the mid 20th century. the crabs were subsequently parasitized by the rhizocephalan heterosaccus dollfusi , itself an erythrean alien. in may 2008, the crab populations were sampled at the southern (israel) and northern (turkey) limits of its introduced range. the prevalence of infection was 3 times as high, and multiple externae-bearing hosts more than 4 tim ... | 2009 | 19530888 |
| molecular cloning and characterization of a heat shock protein 70 gene in swimming crab (portunus trituberculatus). | hsp70 can stimulate cells of the innate immune system directly by acting as "danger"-signaling molecules. to understand the immune defense mechanisms of swimming crab portunus trituberculatus (decapoda: brachyura: portunidae), the cdna of hsp70 (designated pthsp70) was cloned by the combination of homology cloning and rapid amplification of cdna ends (race). the full-length pthsp70 cdna was 2195 bp, including an open reading frame (orf) of 1950 bp encoding a polypeptide of 650 amino acids with e ... | 2010 | 19815077 |
| molecular cloning and characterization of prophenoloxidase gene in swimming crab portunus trituberculatus. | phenoloxidase (po), a melanin-synthesizing enzyme, found as a zymogen (propo) in hemolymp, plays an important role in arthropod defence. in this study, a prophenoloxidase (propo) cdna was cloned from the haemocytes of swimming crab portunus trituberculatus by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end (race) method. analysis of the nucleotide sequence revealed that the cdna clone had a full-length of 3040 bp, with an open reading frame of 2019 ... | 2010 | 19835959 |
| bioinformatic analyses of the publicly accessible crustacean expressed sequence tags (ests) reveal numerous novel neuropeptide-encoding precursor proteins, including ones from members of several little studied taxa. | ests have been generated for many crustacean species, providing an invaluable resource for peptide discovery in members of this arthropod subphylum. here, these data were mined for novel peptide-encoding transcripts, with the mature peptides encoded by them predicted using a combination of online peptide prediction programs and homology to known arthropod sequences. in total, 70 mature full-length/partial peptides representing members of 16 families/subfamilies were predicted, the vast majority ... | 2010 | 20064519 |
| molecular cloning, characterization and mrna expression of two antibacterial peptides: crustin and anti-lipopolysaccharide factor in swimming crab portunus trituberculatus. | in this study, the cdnas of a crustin and an anti-lipopolysaccharide factor (alf) were cloned from the hemocytes of swimming crab portunus trituberculatus using rapid amplification of cdna ends (race) approaches. the full-length cdna of p. trituberculatus crustin (denoted as ptcrustin) was of 584 bp, including an open reading frame (orf) of 333 bp encoding a 21 amino acids signal peptide and a mature polypeptide of 89 amino acids with the predicted molecular mass of 10.08 kda. the typical conser ... | 2010 | 20167286 |
| the fine structure of the hypertrophont of the parasitic apostome synophrya (ciliophora, apostomatida). | the hypertrophont stage of the parasitic apostome ciliate synophrya was studied by light microscopy and transmission electron microscopy. this invasive stage of the ciliate was found within the gill lamellae and gill raphes of the longspine swimming crab portunus spinicarpus. the ciliates elicited a melanized host reaction that walled off the parasite from the host. additionally the ciliate produced a cyst wall of approximately 0.16-2.0 microm in thickness that further isolated the parasite. a m ... | 2010 | 20418077 |
| molecular cloning and characterization of a novel c-type lysozyme gene in swimming crab portunus trituberculatus. | lysozymes are key proteins to invertebrates in the innate immune responses against bacterial infections. in this study, the full-length cdna of a novel c-type lysozyme was cloned from the haemocytes of swimming crab portunus trituberculatus using rapid amplification of cdna ends (race) approaches. the full-length cdna of lysozyme was of 984 bp, containing a 5' untranslated region (utr) of 90 bp, a 3' utr of 222 bp with a poly (a) tail, and an open reading frame (orf) of 672 bp encoding an 18 ami ... | 2010 | 20420912 |
| molecular cloning and characterization of a serine proteinase homolog prophenoloxidase-activating factor in the swimming crab portunus trituberculatus. | serine proteinase homologues (sphs), as one of prophenoloxiase-activating factors (ppafs), play critical roles in innate immunity of crabs. based on an est from the eyestalk full length cdna library, the complete cdna (designated as ptsph) and genomic dna of sph from the swimming crab portunus trituberculatus were cloned in this study. the estimated molecular weight of mature ptsph (354 amino acids) was 38.7 kda and its isoelectric point was 5.08. multiple sequence alignment revealed that ptsph ... | 2010 | 20633654 |
| [cdna cloning, characterization and mrna expression of a profilin from the swimming crab portunus trituberculatus]. | we isolated and characterized the profilin full-length cdna from hemocytes of swimming crab portunus trituberculatus. the profilin cdna consists of 742 bp and the 375 bp open reading frame encodes a polypeptide of 125 amino acids, having a predicted isoelectric point of 5.87. the deduced amino acid sequence shows 42.9% amino acid sequence identity to the profilin of mosquito anopheles gambiae. the profilin mrna was highly expressed in hemocytes and moderately in hepatopancreas of normal crab. th ... | 2010 | 20672413 |
| the invasion of five alien species in the delta do parnaíba environmental protection area, northeastern brazil. | marine biological invasions have been regarded as one of the major causes of native biodiversity loss, with shipping and aquaculture being the leading contributors for the introductions of alien species in aquatic ecosystems. in the present study, five aquatic alien species (one mollusk, three crustaceans and one fish species) were detected during dives, shore searches and from the fisheries on the coast of the delta do parnaíba environmental protection area, in the states of piauí and maranhão, ... | 2010 | 20737846 |
| post-release predation on hatchery-reared japanese flounder paralichthys olivaceus in the coast of fukushima, japan. | to determine the predators of 100 mm total length hatchery-reared juvenile japanese flounder paralichthys olivaceus, fishes and crabs were collected using gillnets and a small trawl net off the coast of fukushima prefecture, japan. predation on juvenile p. olivaceus by older conspecifics, the snailfish liparis tanakai, ocellate spot skate okamejei kenojei and the swimming crab ovalipes punctatus, was detected based on analogical observation and molecular techniques. these predators are nocturnal ... | 2009 | 20738512 |
| molecular characterization and expression analysis of extracellular copper-zinc superoxide dismutase gene from swimming crab portunus trituberculatus. | an extracellular cuznsod cdna was cloned from the haemocytes of swimming crab portunus trituberculatus by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end (race) method. analysis of the nucleotide sequence revealed that the eccuznsod full-length cdna consisted of 965 bp with an open reading frame of 579 bp. it encoded a polypeptide of 192 amino acids which had a predicted molecular weight of 20.0 kda and with an estimated pi of 6.23. the deduced amin ... | 2011 | 20848212 |
| three isoforms of anti-lipopolysaccharide factor identified from eyestalk cdna library of swimming crab portunus trituberculatus. | anti-lipopolysaccharide factors (alfs), as the potent antimicrobial peptides, can bind and neutralize lipopolysaccharide (lps) and exhibit broad spectrum antimicrobial activities. in this study, three isoforms of the alf homologues (ptesalf1-3) were identified from eyestalk cdna library of swimming crab portunus trituberculatus. the full-length cdna sequences of ptesalf1, 2 and 3 were 1138, 1052 and 1057 bp encoding 92, 108 and 123 amino acids, respectively. ptesalf1-3 contained two conserved cy ... | 2010 | 21168510 |
| [cloning and expression pattern analysis of a lipopolysaccharide -and beta-1,3-glucan-binding protein in portunus trituberculatus]. | the lipopolysaccharide -and beta-1,3-glucan-binding protein (lgbp) is a pattern recognition receptor, which is fundamental for the innate immune response of crustaceans. a lgbp gene was cloned from the haemocytes of portunus trituberculatus using smart race methods. the full-length lgbp cdna (1 378 bp) had a 1 095 bp open reading frame encoding a protein of 365 amino acid residues including a 16 amino acid residues signal peptide, a 138 bp 5' untranslated region (utr) and a 144 bp untranslated r ... | 2010 | 21174347 |
| concentrations of total and inorganic arsenic in fresh fish, mollusks, and crustaceans from the gulf of thailand. | concentrations of total and inorganic arsenic were determined in 120 samples of eight marine animals collected from the gulf of thailand between march and may 2008. two species with the highest annual catch from each of four marine animal groups were analyzed: fish (indo-pacific mackerel and goldstripe sardine), bivalves (green mussel and blood cockle), cephalopods (pharaoh cuttlefish and indian squid), and crustaceans (banana prawn and swimming crab). concentrations of inorganic arsenic based o ... | 2011 | 21375883 |
| permanent genetic resources added to molecular ecology resources database 1 may 2009-31 july 2009. | this article documents the addition of 512 microsatellite marker loci and nine pairs of single nucleotide polymorphism (snp) sequencing primers to the molecular ecology resources database. loci were developed for the following species: alcippe morrisonia morrisonia, bashania fangiana, bashania fargesii, chaetodon vagabundus, colletes floralis, coluber constrictor flaviventris, coptotermes gestroi, crotophaga major, cyprinella lutrensis, danaus plexippus, fagus grandifolia, falco tinnunculus, fle ... | 2009 | 21564933 |
| Morphological and Molecular Characterization of Hematodinium perezi (Dinophyceae: Syndiniales), a Dinoflagellate Parasite of the Harbour Crab, Liocarcinus depurator. | Hematodinium perezi Chatton and Poisson (Dinophyceae: Syndiniales) is reported from one of its type hosts, Liocarcinus depurator, from Rye Bay in the English Channel, a site in a similar geographical location to that of the type description. The histology and ultrastructure of vegetative trophont stages, and rDNA sequences of the parasite infecting this host are reported for the first time. Ultrastructurally, H. perezi was confirmed by the presence of condensed chromatin profiles, trichocysts, a ... | 2011 | 22092696 |
| The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): Characterization and potential role in immune response to bacteria and fungi. | Among all the serine proteinase inhibitor families (SPIs), the pacifastin-related inhibitor is seldom isolated. A pacifastin-related SPI named as PtPLC was identified from the swimming crab Portunus trituberculatus in this study. The full-length of PtPLC was cloned from haemocytes cDNA library by the combination of homology cloning and rapid amplification of cDNA ends (RACE). The PtPLC contained an open reading frame (ORF) of 1098 bp encoding a putative pacifastin-related precursor of 365 amino ... | 2012 | 22154999 |
| Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2011-30 September 2011. | This article documents the addition of 299 microsatellite marker loci and nine pairs of single-nucleotide polymorphism (SNP) EPIC primers to the Molecular Ecology Resources (MER) Database. Loci were developed for the following species: Alosa pseudoharengus, Alosa aestivalis, Aphis spiraecola, Argopecten purpuratus, Coreoleuciscus splendidus, Garra gotyla, Hippodamia convergens, Linnaea borealis, Menippe mercenaria, Menippe adina, Parus major, Pinus densiflora, Portunus trituberculatus, Procontar ... | 2012 | 22136175 |
| Cloning and expression of arginine kinase from a swimming crab, Portunus trituberculatus. | Arginine kinase (AK) is an important phosphotransferase that plays a critical role in energy metabolism in invertebrates. In this paper, the cDNA of AK (designated as PtAK) was identified from the eyestalk cDNA library of swimming crab Portunus trituberculatus. The full-length cDNA was 1,479 bp, containing an open reading frame of 1,074 bp that coded for 357 amino acids. The estimated molecular mass of mature PtAK was 40.30 kDa and theoretical isoelectric point was 6.18. Amino acid sequence alig ... | 2011 | 22101972 |
| identification and characterization of a serine protease inhibitor (ptserpin) in the swimming crab portunus trituberculatus. | serine protease inhibitors (serpins) play a key role in diverse immune biological processes. a serine protease inhibitor (serpin), namely ptserpin, was identified from the haemocyte cdna library of swimming crab portunus trituberculatus. the full-length ptserpin cdna was 1593 bp, including an open reading frame (orf) of 1227 bp encoding a polypeptide of 408 amino acids with estimated molecular mass of 45.048 kda and theoretical isoelectric point of 7.23. predicted tertiary structure of ptserpin ... | 2012 | 22245590 |
| transcriptome analysis of the portunus trituberculatus: de novo assembly, growth-related gene identification and marker discovery. | the swimming crab, portunus trituberculatus, is an important farmed species in china, has been attracting extensive studies, which require more and more genome background knowledge. to date, the sequencing of its whole genome is unavailable and transcriptomic information is also scarce for this species. in the present study, we performed de novo transcriptome sequencing to produce a comprehensive transcript dataset for major tissues of portunus trituberculatus by the illumina paired-end sequenci ... | 2014 | 24722690 |
| cloning and characterization of calreticulin and its association with salinity stress in p. trituberculatus. | calreticulin (crt) is a highly conserved and multifunctional endoplasmic reticulum (er) chaperone protein and plays important roles in salinity stress response. portunus trituberculatus is a commercially important fishery species, and water salinity conditions influence its commercial farming significantly. in order to research the function of calreticulin under salinity stress, the full-length cdna sequence of calreticulin from p. trituberculatus (ptcrt) was firstly cloned and characterized. th ... | 2015 | 25995067 |
| transcriptome analysis of portunus trituberculatus in response to salinity stress provides insights into the molecular basis of osmoregulation. | the swimming crab, portunus trituberculatus, which is naturally distributed in the coastal waters of asia-pacific countries, is an important farmed species in china. salinity is one of the most important abiotic factors that influence not only the distribution and abundance of crustaceans, it is also an important factor for artificial propagation of the crab. to better understand the interaction between salinity stress and osmoregulation, we performed a transcriptome analysis in the gills of por ... | 2013 | 24312639 |
| molecular cloning of heat shock protein 60 (pthsp60) from portunus trituberculatus and its expression response to salinity stress. | heat shock protein 60 (hsp60) is a highly conserved and multi-functional molecular chaperone that plays an essential role in both cellular metabolism and stress response. portunus trituberculatus is an important marine fishery and aquaculture species, and water salinity condition influenced its artificial propagations significantly. in order to investigate the function of p. trituberculatus hsp60 against osmotic stress, p. trituberculatus hsp60 gene was firstly cloned. the full-length cdna of pt ... | 2012 | 22434146 |
| de novo transcriptome analysis of portunus trituberculatus ovary and testis by rna-seq: identification of genes involved in gonadal development. | the swimming crab portunus trituberculatus is a commercially important crab species in east asia countries. gonadal development is a physiological process of great significance to the reproduction as well as commercial seed production for p. trituberculatus. however, little is currently known about the molecular mechanisms governing the developmental processes of gonads in this species. to open avenues of molecular research on p. trituberculatus gonadal development, illumina paired-end sequencin ... | 2015 | 26042806 |
| identification of a novel clip domain serine proteinase (sp-csp) and its roles in innate immune system of mud crab scylla paramamosain. | clip domain serine proteinases and their homologs are involved in the innate immunity of invertebrates. to identify the frontline defense molecules against pathogenic infection, we isolated a novel clip domain serine proteinase (sp-csp) from the hemocytes of mud crab scylla paramamosain. the full-length 1362 bp sp-csp contains a 1155 bp open reading frame (orf) encoding 384 amino acids. multiple alignment analysis showed that the putative amino acid sequence of sp-csp has about 52% and 51% ident ... | 2015 | 26272638 |
| isolation and characterisation of bacillus spp. antagonistic to vibrio parahaemolyticus for use as probiotics in aquaculture. | acute gastroenteritis caused by pathogenic vibrio parahaemolyticus is one of the major factors affecting the development of aquaculture and the safety of seafood. using the antagonism of probiotics against pathogens is an alternative strategy to antibiotics and a common trend to control food-borne pathogenic bacteria. in this study, a total of 249 isolates were isolated from four types of seafood (litopenaeus vannamei, oratosquilla oratoria, mactra veneriformis and portunus trituberculatus) and ... | 2015 | 25737203 |
| ptlgbp, a pattern recognition receptor in portunus trituberculatus involved in the immune response against different challenges. | lipopolysaccharide and b-1,3-glucan binding protein (lgbp) is a pattern recognition receptor that can recognize and bind lps and b-1,3-glucan. lgbp has crucial roles in innate immune defense against gram-negative bacteria and fungi. in this study, lgbp functions in portunus trituberculatus innate immunity were analyzed. first, the mrna expression of ptlgbp in hemocytes, hepatopancreas, and muscle toward three typical pathogen-associated molecular patterns (pamps) stimulations were examined using ... | 2014 | 25194733 |
| characterization and functional analysis of a novel c-type lectin from the swimming crab portunus trituberculatus. | c-type lectins (ctls) are a family of calcium-dependent carbohydrate-binding proteins. in the present study, a novel c-type lectin (designated as ptctl1) was identified and characterized from portunus trituberculatus. the full-length cdna of ptctl1 was of 702 bp, containing a 5' untranslated region (utr) of 91 bp, a 3' utr of 110 bp with a poly (a) tail, and an open reading frame (orf) of 501 bp encoding a polypeptide of 166 amino acids with a putative signaling peptide of 21 amino acids. a c-ty ... | 2017 | 28288910 |
| ptplc, a pacifastin-related inhibitor involved in antibacterial defense and prophenoloxidase cascade of the swimming crab portunus trituberculatus. | pacifastin-related inhibitor is a new family of serine protease inhibitors that regulate the proteolytic cascade in multiple biological processes. contrary to the knowledge on the structure and inhibitory mechanism of pacifastin-like members in locust, very little is known about their functions. here, we report the inhibitory activities in relation to the structural characteristics of pacifastin light chain (ptplc) gene identified from the swimming crab portunus trituberculatus. the mature ptplc ... | 2015 | 25542376 |
| ptserpin from the swimming crab portunus trituberculatus, a putative regulator of prophenoloxidase activation with antibacterial activity. | serpin or serine protease inhibitor is the largest family of protease inhibitors involved in many innate immune pathways, particularly the prophenoloxidase (propo) activating system in arthropod. here, we report the molecular and functional characterization of ptserpin identified from the swimming crab portunus trituberculatus. the genomic sequence encoding mature peptide of ptserpin gene contained two exons of 84 and 1098 bp separated by one intron of 111 bp. the recombinant ptserpin (rptserpin ... | 2014 | 24861890 |
| crustins from eyestalk cdna library of swimming crab portunus trituberculatus: molecular characterization, genomic organization and expression analysis. | crustins are cationic, cysteine-rich antimicrobial proteins, containing a single whey acidic protein (wap) domain in the c-terminal end. different from the reported ptcrustin in the hemocytes, two novel crustin genes (ptcrustin2 and ptcrustin3) were cloned completely from the eyestalk cdna library of portunus trituberculatus in this study. all ptcrustins share the consensus cysteine motif and are considered as type i crustins. four exons and three introns are identified in genomic dna sequence o ... | 2012 | 22971359 |
| first report of two thioredoxin homologues in crustaceans: molecular characterization, genomic organization and expression pattern in swimming crab portunus trituberculatus. | previously, we had reported two homologues of the thioredoxin (trx) super-family (pttrx1 and pttrx2) identified from eyestalk and haemocytes cdna library of swimming crab portunus trituberculatus, respectively. it was the first report of two thioredoxin homologues from the same crustacean species. here, we focused on the molecular characterization, genomic organization and expression pattern of pttrx1 and pttrx2. the full-length cdna sequences of pttrx1 and pttrx2 were 739 and 1300 bp, encoding ... | 2012 | 22365991 |
| identification and characterization of two novel types of non-clip domain serine proteases (ptsp and ptsph1) from cdna haemocytes library of swimming crab portunus trituberculatus. | in our previous studies, five serine proteases containing clip domain were characterized from the swimming crab portunus trituberculatus. to further investigate the characterization and function of serine proteases, one serine protease (ptsp) and one serine protease homolog (ptsph1) without clip domain were identified from haemocytes cdna library in this paper. they both possessed an sp or sp-like domain at the c-terminal. in contrast to ptsp, absence of ser catalytic residue resulted in the los ... | 2012 | 22289714 |
| three clip domain serine proteases (csps) and one clip domain serine protease homologue (csph) identified from haemocytes and eyestalk cdna libraries of swimming crab portunus trituberculatus. | four genes including three clip domain serine proteases (ptcsp1, ptcsp2 and ptcsp3) and one clip domain serine protease homologue (ptcsph) of the swimming crab portunus trituberculatus (decapoda: brachyura: portunidae) were characterized based on analysis of expressed sequence tags from haemocytes and eyestalk cdna libraries. the relative four peptidases, which share high structural similarity to the clip-sps of other arthropod species, appeared to possess a clip domain at the n-terminus and an ... | 2012 | 22270256 |
| molecular cloning, expression pattern and antimicrobial activity of a new isoform of anti-lipopolysaccharide factor from the swimming crab portunus trituberculatus. | a new isoform of anti-lipopolysaccharide factor (ptalf5) was cloned from eyestalk cdna library of swimming crab portunus trituberculatus. the full-length cdna of ptalf5 was 1045 bp encoding 120 amino acids. ptalf5 shared lower amino acid similarity with other alfs, yet it contained the conserved lps-binding domain and was clearly member of the alf family. the genomic fragment of ptalf5 contained two exons separated by one intron. several tandem repeats were found in intron. the mrna transcript o ... | 2012 | 22516300 |
| characterization and functional analysis of serine proteinase and serine proteinase homologue from the swimming crab portunus trituberculatus. | serine proteases (sps), with their homologues (sphs), a family of multifunctional proteins, play a crucial role in innate immune system. in our present study, we made an appropriate correction: serine protease homologue ptcsph (li et al., [1]) obtained from the swimming crab portunus trituberculatus was actually a serine protease and re-designated as ptcsp. sequence analysis revealed ptcsp and ptsp (li et al., [2]) might be encoded by the same genomic locus and generated by alternative splicing ... | 2013 | 23664866 |
| immune response and antioxidant status of portunus trituberculatus inoculated with pathogens. | the white spot syndrome virus (wssv), vibrio parahaemolyticus and v. alginolyticus are serious epidemic pathogen affecting the cultured portunus trituberculatus and resulted in severe economic losses to local farmers. the immune and antioxidant systems are believed to be closely involved in host responses to pathogens in aerobic animals, including crustaceans. in order to explore such host-pathogen interactions in the early stage of infection in p. trituberculatus, the mrna transcript levels of ... | 2017 | 28235638 |
| dietary administration of saponin stimulates growth of the swimming crab portunus trituberculatus and enhances its resistance against vibrio alginolyticus infection. | the immunostimulatory role of dietary saponins on swimming crabs was investigated under field conditions. portunus trituberculatus were fed diets enriched with quillaja saponin (qs) at 150, 300 and 450 mg kg(-1). they had an enhanced growth rate and increased resistance against vibrio alginolyticus compared to crabs not fed with qs. significant effects were observed on the average body weight, percentage weight gain and specific growth rate (p < 0.05). total hemocyte and hyaline cell counts of p ... | 2016 | 27815204 |
| identification of a novel class b scavenger receptor homologue in portunus trituberculatus: molecular cloning and microbial ligand binding. | class b scavenger receptors (srbs), which are present in mammals and insects, have been implicated in a wide range of functions. herein, a novel srb homologue, ptsrb, was cloned from the swimming crab, portunus trituberculatus. ptsrb has 538 amino acid residues, and it consists of two transmembrane regions, a large extracellular loop, and two intracellular tails. a phylogenetic analysis showed that ptsrb distinctly clustered with marsupenaeus japonicas srb-1 and most drosophila srb homologues, i ... | 2016 | 27633673 |
| the immune responses and antioxidant status of portunus trituberculatus individuals with different body weights. | vibrio alginolyticus is a virulent pathogen that affects crab aquacultures. in the present study, the immune responses and antioxidant status of big and small (based on body weight and size) 80-, 100- and 120-day-old specimens of portunus trituberculatus, challenged for 72 h with vibrio alginolyticus, were studied. the total hemocyte count (thc), and phagocytic, prophenoloxidase and phenoloxidase activities, of the big individuals (bis) were higher than those of the small individuals (sis) (p < ... | 2016 | 26952172 |
| three novel toll genes (pttoll1-3) identified from a marine crab, portunus trituberculatus: different tissue expression and response to pathogens. | the toll signaling pathway is one of the most important regulators of the immune response in both vertebrates and invertebrates. herein, three novel toll (pttoll1-3) cdna sequences were cloned from the swimming crab, portunus trituberculatus. pttoll1 has 1003 amino acid residues and consists of an extracellular domain containing 15 leucine-rich repeats (lrrs) and a cytoplasmic toll/interleukin-1 receptor (tir) domain of 139 residues. pttoll2 encodes 1196 peptides, with an extracellular domain co ... | 2015 | 26238349 |
| first description and expression analysis of tumor necrosis factor receptor-associated factor 6 (traf6) from the swimming crab, portunus trituberculatus. | tumor necrosis factor receptor-associated factor 6 (traf6) is a cytoplasmic adapter protein that mediates signals induced by the tumor necrosis factor receptor (tnfr) superfamily and the interleukin-1 receptor (il-1r). in the present study, the full-length cdna of traf6 (pt-traf6) was identified in a marine crab, portunus trituberculatus. pt-traf6 orf is predicted to encode a 599-amino acid protein, including a ring type zinc finger, two traf-type zinc fingers, and a meprin and traf homology (ma ... | 2015 | 25882635 |
| characterization and expression of sphsp60 in hemocytes after challenge to bacterial, osmotic and thermal stress from the mud crab scylla paramamosain. | hsp60 play a crucial role in the process of pathogenic and protective immune responses and is implicated in autoimmune disease. in order to understand the environmental response and immune response of this gene, we cloned a hsp60 (sphsp60) gene from the mud crab scylla paramamosain, localized sphsp60 in hemocytes by in situ hybridization, and detected the expression of sphsp60 after stresses in relation to three housekeeping genes (β-actin, 18s rrna and gapdh). the full-length of the sphsp60 cdn ... | 2013 | 23891593 |
| polymorphisms of anti-lipopolysaccharide factors in the swimming crab portunus trituberculatus and their association with resistance/susceptibility to vibrio alginolyticus. | anti-lipopolysaccharide factor (alf) is an important antimicrobial peptide (amp) that can bind and neutralize major component of gram-negative bacteria cell wall, lipopolysaccharide (lps). seven isoforms of anti-lipopolysaccharide factors (ptalf1-7) were previously identified from the swimming crab portunus trituberculatus in our laboratory. here, polymorphisms of ptalf1-7 were detected and their association with resistance/susceptibility to vibrio alginolyticus (a main gram-negative bacteria ca ... | 2013 | 23567857 |
| molecular cloning, genomic structure and antimicrobial activity of ptalf7, a unique isoform of anti-lipopolysaccharide factor from the swimming crab portunus trituberculatus. | anti-lipopolysaccharide factors (alfs), as the potent antimicrobial peptides, are becoming predominant candidates for potential therapeutic agents of bacterial and viral diseases. in this study, a unique isoform of alf (ptalf7) was identified from hemocytes cdna library of the swimming crab portunus trituberculatus. the ptalf7 cdna contained an open reading frame (orf) of 372 bp encoding 123 amino acids. the deduced peptide of ptalf7 shared high similarity with our previously reported ptalf1-3 b ... | 2013 | 23261507 |
| a newly identified anti-lipopolysaccharide factor from the swimming crab portunus trituberculatus with broad spectrum antimicrobial activity. | anti-lipopolysaccharide factors (alfs), exhibiting binding and neutralizing activities to lipopolysaccharide (lps), are the potent antimicrobial peptides of innate immunity in crustaceans. in this study, a unique isoform of alf (ptalf6) was identified from eyestalk cdna library of the swimming crab portunus trituberculatus. the full-length cdna of ptalf6 was 669 bp encoding 115 amino acids, relatively short to other known alfs. the deduced peptide of ptalf6 was conserved; it contained the signal ... | 2013 | 23257203 |
| cdna cloning, characterization and expression analysis of catalase in swimming crab portunus trituberculatus: cdna cloning and expression analysis of catalase gene of portunus trituberculatus. | catalase is an important antioxidant protein that protects organisms against various oxidative stresses by eliminating hydrogen peroxide. in the present study, a full-length cdna sequence of catalase was cloned from the haemocytes of swimming crab portunus trituberculatus by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end method. the catalase cdna sequence contained 1,851 bp with an open reading frame of 1,551 bp encoding 516 amino acid residues. th ... | 2012 | 23073768 |
| a new anti-lipopolysaccharide factor isoform (ptalf4) from the swimming crab portunus trituberculatus exhibited structural and functional diversity of alfs. | anti-lipopolysaccharide factors (alfs) are the potent antimicrobial peptides that can bind and neutralize lipopolysaccharide (lps). in this study, a new isoform of the alf homologs (ptalf4) was cloned from eyestalk cdna library of swimming crab portunus trituberculatus. ptalf4 shared lower amino acid similarity with other alfs, yet it contained the conserved lps-binding domain and was clearly member of the alf family. the genomic sequence of ptalf4 consisted of three exons interrupted by two int ... | 2012 | 22333564 |
| the molecular characterization of a catalase from chinese mitten crab eriocheir sinensis. | catalase (cat) is an antioxidant enzyme and plays a significant role in the protection against oxidative stress by reducing hydrogen peroxide. the cat cdna of eriocheir sinensis (escat) was cloned via race technique. the complete sequence of escat cdna consisted of a 5' untranslated regions (utr) of 224 bp, a 3' utr of 1287 bp with a poly (a) tail and an open reading frame (orf) of 1542 bp, which encoded a polypeptide of 513 amino acid residues with a calculated molecular mass of approximately 5 ... | 2013 | 23171400 |
| isolation and characterization of pseudoalteromonas sp. from fermented korean food, as an antagonist to vibrio harveyi. | the microbial intervention for sustainable management of aquaculture, especially use of probiotics, is one of the most popular and practical approaches towards controlling pathogens. vibrio harveyi is a well-known pathogenic bacterium, which is associated to a huge economic loss in the aquaculture system by causing vibriosis. the present study is crafted for screening and characterization of anti-vibrio strains, which were isolated from various traditional fermented korean foods. a total of 196 ... | 2014 | 23793257 |
| pharmacokinetics and pharmacodynamics of sulfamethoxazole and trimethoprim in swimming crabs (portunus trituberculatus) and in vitro antibacterial activity against vibrio: pk/pd of smz-tmp in crabs and antibacterial activity against vibrio. | serious bacterial pathogens have recently become a major cause of massive mortality in swimming crabs (portunus trituberculatus). in this study, the antibacterial activity against vibrio and the pharmacokinetics (pk) of sulfamethoxazole (smz)-trimethoprim (tmp) in crabs were estimated to explore the pharmacokinetics/pharmacodynamics (pk/pd) properties of the smz-tmp combination. the in vitro bacteriostatic activity and the anti-vibrio infection activity of the smz-tmp combination at various rati ... | 2016 | 27434870 |
| bciv, a new paralyzing peptide obtained from the venom of the sea anemone bunodosoma caissarum. a comparison with the na+ channel toxin bciii. | sea anemones produce a wide variety of biologically active compounds, such as the proteinaceous neurotoxins and cytolysins. herein we report a new peptide, purified to homogeneity from the neurotoxic fraction of b. caissarum venom, by using gel filtration followed by rp-hplc, naming it as bciv. bciv is a 41 amino acid peptide (molecular mass of 4669 amu) possessing 6 cysteines covalently linked by three disulfide bonds. this toxin has 45 and 48% of identity when compared to apetx1 and apetx2 fro ... | 2006 | 17015047 |
| homing in the swimming crab thalamita crenata: a mechanism based on underwater landmark memory. | we investigated whether thalamita crenata, a swimming crab found on the east african intertidal flats, uses landmarks to locate its refuges. we modified the visual panorama of an intertidal flat, using conspicuous movable objects, and conducted homing trials with the local population of swimming crabs. in the first set of trials, after being moved away from their dens, the crabs were immediately able to find other known dens, using direct shortcut paths. in the second set of trials we moved all ... | 2000 | 10973722 |
| [3h-thymidine and 3h-uridine incorporation into the heart cells of the freshwater crayfish astacus astacus and the swimming crab portunus holsatus]. | dna and rna syntheses in the heart cells of two decapod species were investigated with the aid of electron microscopic autoradiography. isotopes were injected in the cavity of adult animals 4 hours before fixation. 3h-thymidine labeling was found in several satellite cell nuclei and in some particular epicardial cell nuclei. none of myonuclei was labeled. 3h-uridine incorporated in all the nuclei of muscle fibers. satellite cells were labeled with 3h-uridine very slightly, if at all. such a pecu ... | 1988 | 2469240 |
| characterization of the complete mitochondrial genome of portunus pelagicus with implications for phylogenomics. | this study determined the mitochondrial genome structure of the blue swimming crab (portunus pelagicus), and elucidated its phylogenetic relationships among the species within the order decapoda. the complete mitochondrial genome was 16,155 bp long, and contained 13 protein-coding genes, 22 transfer rna genes, 2 ribosomal rna genes, and 1 dna control region. the gene order of the genome was the same as that found within the family portunidae. twenty-three genes were on the heavy strand and 14 we ... | 2016 | 27706563 |
| rapid immunochromatographic test strip to detect swimming crab portunus trituberculatus reovirus. | swimming crab reovirus (scrv) is the causative agent of a serious disease with high mortality in cultured portunus trituberculatus. a rapid immunochromatographic assay (ica) was developed in a competitive assay format and optimized for the detection of scrv. the gold probe-based ica test comprised scrv antigen and goat anti-chicken egg yolk antibody (igy) sprayed onto a nitrocellulose membrane as the test line and control line, respectively. igy-gold complexes were deposited onto the conjugate p ... | 2015 | 26575153 |
| [application of electron spin resonance spectroscopy for the detection of irradiated crustaceans (prawn, shrimp, and crabs)]. | prawn, shrimp and crabs sold in japan are mostly imported from overseas. detection of irradiated crustaceans is very important for quality assurance. in this study, we used esr to detect radiation-induced radicals after irradiation of prawn, shrimp and crabs of major species. no radiation-induced radicals were detected in prawn (black tiger prawn) or shrimp (white leg shrimp). radiation-induced radicals due to hydroxyapatite were detected in the claws of snow crab, red king crab, and swimming cr ... | 2014 | 25743382 |
| complete mitochondrial dna sequence of the swimming crab, portunus trituberculatus (crustacea: decapoda: brachyura). | we determined the complete mitochondrial dna sequence for a swimming crab portunus trituberculatus (miers, 1876) (crustacea: decapoda: brachyura). the entire genome was amplified using a long pcr technique, and the products were subsequently used as templates for direct sequencing using a primer walking strategy. the genome (16,026 bp) contained the same 37 genes (two rrnas, 22 trnas, and 13 proteins) plus the putative control region as found in other arthropods. the gene order of p. tritubercul ... | 2003 | 12853147 |
| an endogenous motor program for sand crab uropods. | a stereotyped pattern of spontaneous, rhythmic bursting in motoneurons of three principal uropod muscles in the sand carb emerita analoga has been recorded from a deafferented chain of the four most-posterior abdominal ganglia. this endogenous motor program resembles the electromyogram pattern recorded from return-stroke and power-stroke muscles in swimming crabs in that (1) latencies of power-stroke bursts and burst periods are positively correlated with each other and (2) durations of power-st ... | 1979 | 458439 |
| a stock assessment of the blue swimming crab portunus pelagicus (linnaeus, 1758) for sustainable management in kung krabaen bay, gulf of thailand. | a stock assessment of blue swimming crabs, portunus pelagicus was conducted with crab gill nets and collapsible crab traps at kung krabaen bay, in the eastern gulf of thailand, from 2008 to 2009. several key indicators show that p. pelagicus population is in crisis. fishing mortality shows an increase to 4.14. the exploitation rate is 0.71, higher than the optimal value of 0.38. the size of the mature females has also decreased from 8.10±0.39 cm to 7.52±1.14 cm. the average fecundity is 0.572×10 ... | 2014 | 25210587 |
| production a monoclonal antibody specific to granulocytes of swimming crab (portunus trituberculatus) and its cross reactivity with other crustaceans. | in this study, a monoclonal antibody (mab) 3f4 specific to granulocytes of swimming crab, portunus trituberculatus, was obtained by immunizing mice with whole haemocytes. mab 3f4 showed strong immunofluorescent reaction with granulocytes, but no reaction with hyalinocytes. the positive cell percentage of granulocytes was 86.3% detected by flow cytometry (fcm). a special antigen with molecular weight of about 26kda was further recognized by mab 3f4 in haemocytes of p. trituberculatus. mab 3f4 als ... | 2016 | 27496746 |
| computational analysis of molt-inhibiting hormone from selected crustaceans. | molt-inhibiting hormone (mih) is a principal endocrine hormone regulating the growth in crustaceans. in total, nine mih peptide sequences representing members of the family penaeidae (penaeus monodon, litopenaeus vannamei, marsupenaeus japonicus), portunidae (portunus trituberculatus, charybdis japonica, charybdis feriata), cambaridae (procambarus bouvieri), parastacidae (cherax quadricarinatus) and varunidae (eriocheir sinensis) were selected for our study. in order to develop a structure based ... | 2013 | 24041714 |
| tumor necrosis factor receptor-associated factor 6 (traf6) participates in anti-lipopolysaccharide factors (alfs) gene expression in mud crab. | tumor necrosis factor receptor-associated factor 6 (traf6) is a key cytoplasm signal adaptor that mediates signals activated by tumor necrosis factor receptor (tnfr) superfamily and the interleukin-1 receptor/toll-like receptor (il-1/tlr) superfamily. the full-length 2492 bp traf6 (sp-traf6) from scylla paramamosain contains 1800 bp of open reading frame (orf) encoding 598 amino acids, including an n-terminal ring-type zinc finger, two traf-type zinc fingers and a conserved c-terminal meprin and ... | 2017 | 27581742 |
| reproductive features of the swimming crab callinectes danae(crustacea, portunoidea) on the subtropical coast of brazil: a sampling outside the estuary. | the life cycle of the crab callinectes danae is estuarine-dependent, and studies on aspects of their biology should also cover marine areas. the present study investigated the sexual maturity, as well as habitat preference by adults in different gonadal stages, and the crabs' reproductive periodicity outside the estuary. three bays on the subtropical southeastern coast of brazil were sampled monthly for two years. for each bay, six transects were established, four of them parallel to the beach l ... | 2015 | 26465730 |
| monomia calla, a new species of swimming crab (decapoda, portunidae) <br />from madagascar and the philippines. | a new species of portunid crab (brachyura: portunidae) from southern madagascar and central philippines is described. monomia calla sp. nov. is most similar in morphology to m. lecromi (moosa, 1996) from the chesterfield islands in the southwestern pacific ocean, and m. rubromarginata (lanchester, 1900) from the south china sea, singapore, malay archipelago and the northern half of australia. the new species can be easily distinguished from all congeners by the distinctive morphology of the male ... | 2015 | 26250003 |
| biological associations of color variation in the indo-pacific swimming crab charybdis hellerii. | a marine biological invasion is a natural process accelerated by human activities, and the crab charybdis hellerii is an example of a globally widespread invasive species. this study evaluated color variation in c. hellerii and its relationship to the sex, size and sexual maturity of these crabs, and compared the efficiency of a freeware digital image-editing program with a commercially available program. the color of the individuals was analyzed using standardized digital images. the color patt ... | 2015 | 25761218 |
| multiple isoforms of immune-related genes from hemocytes and eyestalk cdna libraries of swimming crab portunus trituberculatus. | expressed sequence tags (ests) analysis has been shown to be an efficient approach not only for gene discovery, but also for gene expression profiles performance. two full-length enriched cdna libraries were constructed from hemocytes and eyestalk of portunus trituberculatus, respectively, and randomly sequenced to collect genomic information and identify genes involved in immune defense response. a total of 99 unigenes including 64 unigenes (6.00% of 1066 unigenes) in hemocytes library and 35 u ... | 2011 | 21362485 |
| modulation by ammonium ions of gill microsomal (na+,k+)-atpase in the swimming crab callinectes danae: a possible mechanism for regulation of ammonia excretion. | the modulation by na(+), k(+), nh(4)(+) and atp of the (na(+),k(+))-atpase in a microsomal fraction from callinectes danae gills was analyzed. atp was hydrolyzed at high-affinity binding sites at a maximal rate of v=35.4+/-2.1 umg(-1) and k(0.5)=54.0+/-3.6 nm, obeying cooperative kinetics (n(h)=3.6). at low-affinity sites, the enzyme hydrolyzed atp obeying michaelis-menten kinetics with k(m)=55.0+/-3.0 microm and v=271.5+/-17.2 umg(-1). this is the first demonstration of a crustacean (na(+),k(+) ... | 2002 | 12223203 |
| identification and molecular characterization of a c-type lectin-like protein from chinese shrimp (fenneropenaeus chinensis). | a c-type lectin-like protein was cloned and characterized from the chinese shrimp fenneropenaeus chinensis, and named as fcctl. the results indicated that the full length cdna of 859 bp had an open reading frame encoded a polypeptide of 220 amino acids with one carbohydrate-recognition domain, six conserved cys and one key motif epgd. the theoretical molecular weight and pi of mature protein was 25.3 kda and 5.4. sequence comparison of the deduced amino acid sequence of fcctl showed varied ident ... | 2013 | 23192616 |
| a chymotrypsin-like serine protease from portunus trituberculatus involved in pathogen recognition and amp synthesis but not required for prophenoloxidase activation. | clip domain serine proteases (clip-sps) play critical roles in various immune responses in arthropods, such as hemolymph coagulation, antimicrobial peptide (amp) synthesis, cell adhesion and melanization. in the present study, we report the molecular and functional characterization of a clip domain serine protease (ptcsp2) from the swimming crab portunus trituberculatus. the n-terminal clip domain and the c-terminal sp-like domain of ptcsp2 were expressed in escherichia coli system, and assayed ... | 2017 | 28522421 |
| characterization and antimicrobial evaluation of sppr-amp1, a proline-rich antimicrobial peptide from the mud crab scylla paramamosain. | antimicrobial peptide (amp) is an important molecule in the innate immune system. here, we report the cloning and functional studies of proline-rich amps (pr-amps) from the three species of mud crab: scylla paramamosain, s. serrata, and the swimming crab portunus pelagicus. the deduced peptides revealed that they contain the putative signal peptides and encode for mature peptides, which contain sequence architecture similar to a 6.5-kda proline-rich amp of the shore crab, carcinus maenas which s ... | 2017 | 28479344 |
| characterization of three caspases and their pathogen-induced expression pattern in portunus trituberculatus. | caspases are a family of proteases involved in many important biological processes including apoptosis and inflammation. in this study, we analyzed the expression patterns and effects on immune response in various tissues of the edible crab portunus trituberculatus. ptcas 2, ptcas 3 and ptcas 4 share overall sequence identities of 55.88%-74.86%, 8.47%-46.54% and 20.11%-50.87%, respectively, with their other crustacean species. ptcas 2, ptcas 3 and ptcas 4 have the same caspase domain and catalyt ... | 2017 | 28478258 |
| hematodinium spp. infections in wild and cultured populations of marine crustaceans along the coast of china. | the parasitic dinoflagellate hematodinium spp. infects a broad range of marine crustaceans. its epidemics have impacted wild populations of various commercial fishery species around the world and the sustainability of mariculture in china. to study the epidemiology of hematodinium spp. in marine crustaceans along the coast of china, we conducted a broad survey of wild and cultured stocks of major crustacean species in 2013 to 2015. hematodinium sp. infections were identified in wild stocks of po ... | 2017 | 28492174 |
| [niche and interspecific association of nekton in the south coastal waters of cangnan, zhe-jiang, china]. | in this paper, the interaction between the species population were analyzed based on the data of major nekton species in the south coastal waters of cangnan in spring and autumn 2014. a series of methods including niche breadth, niche overlap, χ2 - test and association coefficient (ac) based on a 2x2 contingency table were used. the results showed that the major nekton species in the south coastal waters of cangnan were classified into three groups, i.e. wide niche breadth species, moderate nich ... | 2015 | 26995931 |
| diversity and characterization of bacteria associated with the deep-sea hydrothermal vent crab austinograea sp. comparing with those of two shallow-water crabs by 16s ribosomal dna analysis. | for deep-sea hydrothermal vent crabs, recent investigations have revealed some epibiotic bacteria, but no study has described the bacterial community associated with the gill and intestine. in this study, the microbiota attached to the gill and intestine of the hydrothermal vent crab austinograea sp. and two shallow-water crab species (eriocheir sinensis and portunus trituberculatus) were compared by high-throughput sequencing of 16s rdna genes. the highest and lowest diversity in bacterial comm ... | 2017 | 29121118 |
| morphology and phylogeny of ameson portunus n. sp. (microsporidia) infecting the swimming crab portunus trituberculatus from china. | ameson portunus n. sp. is a new microsporidian species that infects the skeletal muscle of portunus trituberculatus, a pond-reared swimming crab from china. this parasite was characterized using morphological and molecular phylogenetic data. light and transmission electron microscopy revealed that this microsporidian experienced disporogonic and polysporogonic (chain-like) life cycles. mature uninucleate spores appeared ovoid, measured 1.4±0.06×1.0±0.07μm on ultrathin sections, and exhibited no ... | 2017 | 29055853 |
| taxonomic revision of the speckled crabs, genus <i>arenaeus</i> dana, 1851 (brachyura: portunidae) based on morphological and molecular data. | the family portunidae rafinesque, 1815 presents a series of taxonomic problems such as paraphyletic groups, synonymizations, and unresolved complexes of cryptic species. arenaeus dana, 1851, encompasses only two species with mirrored distributions along the coasts of the americas. despite of comprising two widespread species, there is a scarcity of information on their biology and ecology and on the relationships with other genera in the family. because of the lack of studies comprising both spe ... | 2017 | 28610239 |
| mitochondrial dna variation in the east china sea and yellow sea populations of swimming crab ovalipes punctatus. | swimming crab ovalipes punctatus is a commercially important species in the east china sea and yellow sea, but there is limited knowledge of its genetic population structure. the population genetic structure of o. punctatus in east china sea and yellow sea was examined with a 658-bp segment of the mtdna coi gene. a total of 60 individuals were collected from five locations and 48 haplotypes were obtained. mean haplotype diversity and nucleotide diversity for the five populations were 0.9876 ± 0. ... | 2015 | 24438308 |