| [study on chemical constituents and anti-bacterial activity of volatile oil from seed of aquilaria sinensis]. | volatile oil from the seed of aquilaria sinensis was extracted with petroleum ether. anti-bacterial bioassay of the oil against methicillin-resistant staphylococcus aureus (mrsa) was carried out by paper disco diffusion method. the result showed that the oil possessed inhibitory activity towards mrsa. 19 compounds of the volatile oil were identified by gc-ms analysis. | 2008 | 18619234 |
| laxative effect of agarwood leaves and its mechanism. | we investigated the laxative activity of an extract of agarwood leaves from aquilaria sinensis. the laxative activity was measured in mice by counting the stool frequency and stool weight, and the drugs were orally administered. an acetone extract of agarwood leaves and senna (a representative laxative drug) both increased the stool frequency and weight, but a methanol extract did not. the laxative effect of the acetone extract was milder than that of the anthraquinoid laxative, senna, and the f ... | 2008 | 18256503 |
| micrococcus endophyticus sp. nov., isolated from surface-sterilized aquilaria sinensis roots. | a gram-positive bacterial strain, designated yim 56238(t), was isolated from plant roots (aquilaria sinensis), and characterized by using a polyphasic approach. strain yim 56238(t) grew optimally at ph 7.0-8.0 and at 28 degrees c. analysis of the 16s rrna gene sequence of strain yim 56238(t) indicated that it belongs to the genus micrococcus. chemotaxonomic data strongly supported the classification of this strain within the genus micrococcus: the cell-wall peptidoglycan contained lysine, glutam ... | 2009 | 19406795 |
| antinociceptive and anti-inflammatory activities of aquilaria sinensis (lour.) gilg. leaves extract. | the analgesic and anti-inflammatory activities of the ethanol extract of aquilaria sinensis (lour.) gilg. leaves were observed in various experimental models related to nociception and inflammation, so as to provide some evidence for its traditional use. | 2008 | 18353573 |
| anti-inflammatory 2-(2-phenylethyl)chromone derivatives from chinese agarwood. | five new 2-(2-phenylethyl)chromone derivatives (1-5), along with eleven known compounds (6-16) were isolated from chinese agarwood. their structures were elucidated by spectroscopic data (nmr, uv, ir, and ms) analyses and comparison of their spectroscopic and physical data with the literature values. the absolute configurations of 2-4 were determined by electronic circular dichroism (ecd) calculations. compounds 2-4, 11, 12, and 15 exhibited significant inhibition of nitric oxide production in l ... | 2017 | 28237880 |
| salinity stress induces the production of 2-(2-phenylethyl)chromones and regulates novel classes of responsive genes involved in signal transduction in aquilaria sinensis calli. | agarwood, is a resinous portion derived from aquilaria sinensis, has been widely used in traditional medicine and incense. 2-(2-phenylethyl)chromones are principal components responsible for the quality of agarwood. however, the molecular basis of 2-(2-phenylethyl)chromones biosynthesis and regulation remains almost unknown. our research indicated that salt stress induced production of several of 2-(2-phenylethyl)chromones in a. sinensis calli. transcriptome analysis of a. sinensis calli treated ... | 2016 | 27230436 |
| transcriptome sequencing of chemically induced aquilaria sinensis to identify genes related to agarwood formation. | agarwood is a traditional chinese medicine used as a clinical sedative, carminative, and antiemetic drug. agarwood is formed in aquilaria sinensis when a. sinensis trees are threatened by external physical, chemical injury or endophytic fungal irritation. however, the mechanism of agarwood formation via chemical induction remains unclear. in this study, we characterized the transcriptome of different parts of a chemically induced a. sinensis trunk sample with agarwood. the illumina sequencing pl ... | 2016 | 27182594 |
| chinese vegetative materia medica in a venereological treatise by jean astruc from 1740. | historical medical sources can be still queried for forgotten cures and remedies. traditional chinese medicine has dealt with lues venerea (syphilis) since the five dynasties period (10th century). chinese indigenous materia medica and remedies recorded, studied or imported by the europeans can reveal known or quite unknown medicinal plants. the studied jean astruc's work is a published ethnopharmacological survey carried out in beijing in the 1730s and it deserves a modern interpretation. | 2016 | 27132716 |
| identification and functional characterization of three type iii polyketide synthases from aquilaria sinensis calli. | type iii polyketide synthases (pkss) play an important role in biosynthesis of various plant secondary metabolites and plant adaptation to environmental stresses. aquilaria sinensis (a. sinensis) is the main plant species for production of agarwood, little is known about its pks family. in this study, aschs1 and two new type iii pkss, aspks1 and aspks2, were isolated and characterized in a. sinensis calli. the comparative sequence and phylogenetic analysis indicated that aspks1 and aspks2 belong ... | 2017 | 28366630 |
| sesquiterpenoids from chinese agarwood induced by artificial holing. | two new sesquiterpenoids, 3-oxo-7-hydroxylholosericin a (1) and 1,5;8,12-diepoxy-guaia-12-one (2), together with seven known sesquiterpenoids 3-9, were isolated from chinese agarwood induced by artificial holing originating from aquilaria sinensis (lour.) gilg. their structures were identified by spectroscopic techniques (uv, ir, 1d and 2d nmr) and ms analyses. the absolute configuration of compound 1 was determined by comparison of its measured cd curve with that of calculated data for 1 and en ... | 2016 | 26927047 |
| identification of genes related to agarwood formation: transcriptome analysis of healthy and wounded tissues of aquilaria sinensis. | agarwood is an expensive resinous heartwood derived from aquilaria plants that is widely used in traditional medicines, incense and perfume. only wounded trees can produce agarwood, and the huge demand for the agarwood products has led all aquilaria spp. being endangered and listed in the appendix ii of the cites (http://www.cites.org). the major components of agarwood are sesquiterpenes and phenylethyl chromones. owing to a lack of genomic information, the molecular basis of wound-induced sesqu ... | 2013 | 23565705 |
| a novel degraded sesquiterpene from the fresh stem of aquilaria sinensis. | a novel degraded sesquiterpene, named aquilarin b (1), together with two known compounds (2 and 3), was isolated from the etoh extract of the fresh stem of aquilaria sinensis (lour.) gilg. their structures were elucidated by spectroscopic methods including 1d and 2d nmr (hmqc, (1)h-(1)h cosy, hmbc, and roesy). the cytotoxic activities of the three compounds against three human tumor cell lines k562, smmc-7721, and sgc-7901 were evaluated, and compound 3 exhibited obvious cytotoxic activity. | 2011 | 21972811 |
| comparison of compositions and antimicrobial activities of essential oils from chemically stimulated agarwood, wild agarwood and healthy aquilaria sinensis (lour.) gilg trees. | the composition and antimicrobial activity of the essential oils which were obtained from agarwood originated from aquilaria sinensis (lour.) gilg stimulated by the chemical method (s1) were characterized, taking wild agarwood (s2) and healthy trees (s3) respectively as the positive and negative controls. the chemical composition of s1 was investigated by gas chromatography-mass spectrometry (gc-ms). the essential oil of s1 showed a similar composition to that of s2, being rich in sesquiterpenes ... | 2011 | 21677602 |
| anti-neuroinflammatory sesquiterpenes from chinese eaglewood. | nine new sesquiterpenes (1-9), together with seventeen known ones (10-26), were isolated from chinese eaglewood. their structures were established by extensive spectroscopic analysis, and the absolute configuration of 6 was determined by the modified mosher's method. compounds 7, 10, 14, 15, and 21 exhibited significant inhibition of nitric oxide production in lipopolysaccharide-stimulated bv-2 microglial cells with ic50 values in the range 7.1-53.8 μm. | 2015 | 26304765 |
| a novel sesquiterpene alcohol from fimetariella rabenhorstii, an endophytic fungus of aquilaria sinensis. | a novel sesquiterpene alcohol, named frabenol (1), was isolated from liquid cultures of fimetariella rabenhorstii a20, an endophytic fungus of the agarwood-forming plant aquilaria sinensis (lour.) gilg. the structure of the new compound was elucidated on the basis of spectroscopic methods. the finding of a sesquiterpenoid compound in f. rabenhorstii a20 implied that endophytic fungi of agarwood-producing plants could also contribute to the generation of fragrant chemicals during the agarwood for ... | 2011 | 21815406 |
| [study on the chemical constituents of endophytic fungus fimetariella rabenhorstii isolated from aquilaria sinensis]. | to study the chemical constituents of endophytic fungus fimetariella rabenhorsti isolated from aquilaria sinensis. | 2011 | 21823477 |
| antitumor and antimicrobial activities of endophytic fungi from medicinal parts of aquilaria sinensis. | the purpose of this study was to isolate and characterize endophytic fungi from the stem tissue which can produce fragrant ingredients in aquilaria sinensis (also called agarwood) to determine their antitumor and antimicrobial activities. twenty-eight fungal endophytes were isolated from agarwood by strict sterile sample preparation and were classified into 14 genera and 4 taxonomic classes (sordariomycetes, dothideomycetes, saccharomycetes, and zygomycetes) based on molecular identification. of ... | 2011 | 21528493 |
| structural elucidation of two new megastigmane glycosides from the leaves of aquilaria sinensis. | the present study was designed to determine the chemical constituents and identify new components of the leaves of aquilaria sinensis (lour.) gilg. the compounds were isolated and purified by repeated silica gel, sephadex lh-20, and ods column chromatography and their structures were elucidated by nmr and hr-esi-ms spectrometry. eight megastigmane glycosides and two cucurbitacins were isolated and identified as (9s) megastigma-4,7-diene-2,3,9-triol 9-o-β-d-glucopyranoside (1), (9s) megastigma-4( ... | 2015 | 25908626 |
| effects of iriflophenone 3-c-β-glucoside on fasting blood glucose level and glucose uptake. | one of the biological activities of agar wood (aquilaria sinensis lour., thymelaeaceae), is anti-hyperglycemic activity. the methanolic extract (me) was proven to possess the fasting blood glucose activity in rat and glucose uptake transportation by rat adipocytes. | 2017 | 25709215 |
| rapid preparation of (methyl)malonyl coenzyme a and enzymatic formation of unusual polyketides by type iii polyketide synthase from aquilaria sinensis. | (methyl)malonyl coenzyme a was rapidly and effectively synthesized by a two-step procedure involving preparation of n-hydroxysuccinimidyl (methyl)malonate from (methyl)meldrum's acid, and followed by transesterification with coenzyme a. the synthesized (methyl)malonyl coenzyme a could be well accepted and assembled to 4-hydroxy phenylpropionyl coenzyme a by type iii polyketide synthase from aquilaria sinensis to produce dihydrochalcone and 4-hydroxy-3,5-dimethyl-6-(4-hydroxyphenethyl)-2h-pyrone ... | 2015 | 25677661 |
| [study on volatile components from peel of aquilaria sinensis and the anti-tumor activity]. | to study the volatile constituents from peel of aquilaria sinensis (lour.) gilg. and its anti-tumor activity. | 2010 | 21434436 |
| agarwood induced laxative effects via acetylcholine receptors on loperamide-induced constipation in mice. | agarwood (aquilaria sinensis, aquilaria crasna) is well known as an incense in the oriental region such as thailand, taiwan, and cambodia, and is used as a digestive in traditional medicine. we investigated the laxative effects and mechanism of agarwood leaves extracted with ethanol (eea-1, aquilaria sinensis; eea-2, aquilaria crasna). eea-1, eea-2, the main constituents of eeas (mangiferin, and genkwanin-5-o-primeveroside), and senna increased the frequency and weight of stools in loperamide-in ... | 2010 | 20699592 |
| flavonoid and a rare benzophenone glycoside from the leaves of aquilaria sinensis. | from the leaves of aquilaria sinensis (lour.) gilg, a novel benzophenone glucoside, designated as aquilarinoside a (1), and a new flavonoid, as 7-beta-d-glucoside of 5-o-methylapigenin (2), along with eight known compounds (3-10) were isolated. the structures of 1 and 2 were fully characterized by spectroscopic methods (1d, 2d nmr and ms) and the relative stereochemistry was assigned based on nuclear overhauser effect spectroscopy (noesy) correlations and analyses of coupling constants. the new ... | 2009 | 19182401 |
| qinanmer, a new compound from chinese agarwood 'qi-nan' originating from aquilaria sinensis. | qinanmer (1), a new compound comprising 2-(2-phenylethyl)chromone and sesquiterpene moieties, together with two known 2-(2-phenylethyl)chromone derivatives (2-3), was isolated from the high-quality chinese agarwood "qi-nan" originating from aquilaria sinensis (lour.) glig. the structure of 1 was elucidated by spectroscopic techniques (uv, ir, 1d and 2d nmr), ms analyses, ecd spectra analyses, and quantum (13)c nmr calculations. | 2017 | 28043172 |
| four new 2-(2-phenylethyl)chromone derivatives from chinese agarwood produced via the whole-tree agarwood-inducing technique. | four new 2-(2-phenylethyl)chromone derivatives (1-4) were isolated from the etoh extract of chinese agarwood produced via the whole-tree agarwood-inducing technique, coming from aquilaria sinensis (lour.) spreng. (thymelaeaceae). their structures were elucidated by extensive spectroscopic methods, such as uv, ir, ms, 1d as well as 2d nmr. all of the isolates were then assessed for their anti-inflammatory activities on lipopolysaccharide (lps)-induced nitric oxide (no) production in raw 264.7. co ... | 2016 | 27801808 |
| profiling of micrornas under wound treatment in aquilaria sinensis to identify possible micrornas involved in agarwood formation. | agarwood, a kind of highly valued non-timber product across asia, is formed only when its resource trees--the endangered genus aquilaria are wounded or infected by some microbes. to promote the efficiency of agarwood production and protect the wild resource of aquilaria species, we urgently need to reveal the regulation mechanism of agarwood formation. micrornas (mirnas) are a group of gene expression regulators with overwhelming effects on a large spectrum of biological processes. however, thei ... | 2014 | 24795531 |
| five 2-(2-phenylethyl)chromones from sodium chloride-elicited aquilaria sinensis cell suspension cultures. | five 2-(2-phenylethyl)chromones including a new one, (5s,6r,7s,8r)-5,8-dichloro-6,7-dihydroxy-2-phenylethyl-5,6,7,8-tetrahydro-4h-chromen-4-one (1), and four known ones (2-5), were isolated from 150 mm nacl-elicited aquilaria sinensis cell suspension cultures. in addition, three feruloyl amides (6-8), six nucleosides (9-14), (+)-syringaresinol (15), indole-3-carboxaldehyde (16), and two glycosides (17-18) were also obtained. the structures were unambiguously identified by analysis of their uv, i ... | 2016 | 27128895 |
| complete chloroplast genome sequence of aquilaria sinensis (lour.) gilg and evolution analysis within the malvales order. | aquilaria sinensis (lour.) gilg is an important medicinal woody plant producing agarwood, which is widely used in traditional chinese medicine. high-throughput sequencing of chloroplast (cp) genomes enhanced the understanding about evolutionary relationships within plant families. in this study, we determined the complete cp genome sequences for a. sinensis. the size of the a. sinensis cp genome was 159,565 bp. this genome included a large single-copy region of 87,482 bp, a small single-copy reg ... | 2016 | 27014304 |
| [further chemical investigation of leaves of aquilaria sinensis]. | to comprehensively utilize the rich resource of aquilaria sinensis,the ethanol extract of the leaves of a. sinensis was further chemically investigated, which led to the isolation of 12 compounds. by means of 'h- , "c-nmr, and esi-ms data, and through comparison with those reported in literatures,their structures were identified as iriflophenone 2-(o-a-l)-rhamnoside(1) ,4'-hydroxy-5 methoxyflavone-7-o-glucoxyloside (2) ,7,3',5'-tri-o-methyltricetin(3) ,7-o-beta-d-glucopyranoside of 5-o-methylapi ... | 2013 | 24422396 |
| [cloning and expression analysis of farnesyl pyrophosphate synthase from aquilaria sinensis]. | farnesyl diphosphate synthase (fps) is one of the key rate-limiting enzymes in the sesquiterpene metabolic pathways. in this study, the open reading frame (orf) of fps was cloned by pcr based on the transcript sequence of asfps1 from the aquilaria sinensis transcriptome database and sequenced. total rna was extracted from the root, stem and leaves of three-year-old a. sinensis, and from healthy and wounded a. sinensis calli, and then reverse-transcribed into single-stranded cdna as a template fo ... | 2013 | 24422386 |
| [high-quality total rna extraction from medicinal plant aquilaria sinensis]. | it is quite difficult to isolate total rna from aquilaria sinensis due to the complexity of its secondary metabolites. in order to obtain the high-quality rna, we investigated sampling method and rna extraction from both normal and agilawood tissues of the plant. | 2013 | 24417137 |
| [chemical constituents from stems of aquilaria sinensis]. | to study the constituents from the stems of aquilaria sinensis. | 2013 | 24380305 |
| identification and quantification of 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones in chinese eaglewood by hplc with diode array detection and ms. | a sensitive and reliable hplc coupled with diode array detection and ms method was developed and validated for the first time to simultaneously identify and quantify eight characteristic 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones (thpecs) in chinese eaglewood. chromatographic separation was performed on a zorbax sb c18 column with a gradient of acetonitrile/0.1% formic acid/water as the mobile phase. the ms fragmentation behavior of thpecs was characterized as the successive neutral loss of t ... | 2013 | 24115601 |
| 2-(2-phenylethyl)chromone derivatives from chinese agarwood induced by artificial holing. | three new 2-(2-phenylethyl)chromone derivatives (1-3), together with thirteen known ones (4-16), were isolated from the etoac extract of chinese agarwood induced by artificial holing, originating from aquilaria sinensis (lour.) gilg (thymelaeaceae). the chemical structures of the new compounds were identified by spectroscopic techniques (uv, ir, ms, 1d and 2d nmr). compounds 1, 6, 15 and 16 exhibited inhibitory effects on staphylococcus aureus, and compounds 15 and 16 showed inhibitory effects o ... | 2014 | 25068202 |
| five new eudesmane-type sesquiterpenoids from chinese agarwood induced by artificial holing. | five new eudesmane-type sesquiterpenoids (1-5), along with six known ones (6-11), were isolated from chinese agarwood induced by artificial holing originating from aquilaria sinensis (lour.) gilg (thymelaeaceae). the structures of the new sesquiterpenoids were established by spectroscopic methods including uv, ir, ms, 1d, and 2d nmr. compounds 1, 3, 6 and 7 exhibited antibacterial activities against both staphylococcus aureus and ralstonia solanacearum, and compound 5 only showed an inhibitory a ... | 2015 | 25447164 |
| solvent effects and improvements in the deoxyribose degradation assay for hydroxyl radical-scavenging. | the deoxyribose degradation assay is widely used to evaluate the hydroxyl (oh) radical-scavenging ability of food or medicines. we compared the hydroxyl radical-scavenging activity of 25 antioxidant samples prepared in ethanol solution with samples prepared after removing the ethanol (residue). the data suggested that there was an approximately 9-fold difference between assay results for the ethanol solution and residue samples. this indicated a strong alcoholic interference. to further study th ... | 2013 | 23870931 |
| [cloning and bioinformatics analysis of chalcone synthase (aschs1) gene in aquilaria sinensis]. | the study aimed to clone the open reading frame of chalcone synthase (chs) from aquilaria sinensis and analyze the bioinformatics and expression of the gene. | 2013 | 23672032 |
| three new sesquiterpenoids and one new sesquiterpenoid derivative from chinese eaglewood. | three new sesquiterpenoids (1-3) and one new sesquiterpenoid derivative (4), along with three known sesquiterpenoids (5-7), were isolated from the 95% ethanolic extract of chinese eaglewood [aquilaria sinensis (lour.) gilg]. the structures of these compounds were elucidated through extensive analysis of spectroscopic data including ir, nmr, hresims, and x-ray diffraction experiments. in addition, the above new compounds were detected for their bioactivities against lps-induced no production in r ... | 2016 | 26927050 |
| [identification of pathogen of aquilaria sinensis dieback disease]. | the research focused on identification the pathogen of aquilaria sinensis dieback disease and confirmation the function of inducing agarwood formation by fungi. | 2013 | 24010282 |
| qualitative and quantitative analysis of phenolic compounds in the leaves of aquilaria sinensis using liquid chromatography-mass spectrometry. | the leaves of aquilaria sinensis traditionally have been used in china for a century. phenolic compounds were investigated to be the major active compounds in them. | 2013 | 23483592 |
| whole-tree agarwood-inducing technique: an efficient novel technique for producing high-quality agarwood in cultivated aquilaria sinensis trees. | agarwood is the fragrant resin-infused wood derived from the wounded trees of aquilaria species. it is a valuable non-timber forest product used in fragrances and as medicine. reforestation for aquilaria trees in combination with artificial agarwood-inducing methods serves as a way to supply agarwood and conserve of wild aquilaria stock. however, the existing agarwood-inducing methods produce poor-quality agarwood at low yield. our study evaluated a novel technique for producing agarwood in cult ... | 2013 | 23470337 |
| [study of production of sesquiterpenes of aquilaria senensis stimulated by lasiodiplodia theobromae]. | to investigate the mechanism of agarwood formation in aquilaria sinensis induced by lasiodiplodia theobromae, the fermentation liquor of l. theobromae was analyzed qualitatively and quantitatively by gas chromatography-mass spectrometry (gc-ms). jas were detected in the fermentation liquor. the effect of the fermentation liquor on the abundance of sesquiterpenes in the callus of a. sinensis was analyzed by solid phase microextraction-gas chromatography-mass spectrometry (spme-gc-ms). and the fer ... | 2014 | 24761630 |
| [sesquiterpenes and monoterpene from aquilaria sinensis]. | the column chromatography on silica gel, semi-preparative hplc were used to separate and purify the compounds from the petroleum ether and ethanol extract of aquilaria sinensis. nine compounds were isolated. on the basis of their spectroscopic data, the structures were identified as 3, 3, 7-trimethyltricycloundecan-8-one (1), longifolene (2), norlongilactone (3), caryophyllenol-ii (4), humulene diepoxide a (5), kobusone (6), (-)-bornyl ferulate (7), (24r) -24-ethylcholesta-4, 22-dien-3-one (8), ... | 2012 | 23019881 |
| [cloning, prokaryotic expression, and functional identification of a sesquiterpene synthase gene (asss4) from aquilaria sinensis]. | a sesquiterpene synthase (asss4) full-length open reading frame (orf) cdna was cloned from wounded stems of aquilaria sinensis by rt-pcr method. the result showed that the orf of asss4 was 1,698 bp encoding 565 amino acids. prokaryotic expression vector pet28a-asss4 was constructed and transformed into e. coli bl21 (de3) plyss. recombinant asss4 protein was obtained after induction by iptg and sds-page analysis with a mw of 64 kd. enzymatic reactions using farnesyl pyrophosphate showed that reco ... | 2014 | 25920204 |
| agarwood formation induced by fermentation liquid of lasiodiplodia theobromae, the dominating fungus in wounded wood of aquilaria sinensis. | agarwood is broadly used in incense and medicine. traditionally, agarwood formation is induced by wounding the trunks and branches of some species of aquilaria spp., including a. sinensis. as recently evidenced, some fungi or their fermentation liquid may have the potential of inducing agarwood formation. the present study aimed to analyze the fungi isolated from an agarwood-producing a. sinensis tree and subsequently identify the fungi capable of promoting agarwood formation. we identified a to ... | 2017 | 28224223 |
| [studies on the constituents of aquilaria sinensis (lour.) gilg. i. isolation and structure elucidation of two new sesquiterpenes, baimuxinic acid and baimuxinal]. | | 1983 | 6615594 |
| aquisiflavoside, a new nitric oxide production inhibitor from the leaves of aquilaria sinensis. | a new flavonoid glycoside, named aquisiflavoside, was isolated from the leaves of aquilaria sinensis (lour.) gilg, and its structure was elucidated by spectroscopic analyses. aquisiflavoside was evaluated for the inhibition of nitric oxide (no) production induced by lipopolysaccharide in macrophage cell line raw 264.7 and exhibited a potent inhibitory activity against no production in a dose-dependent manner with an ic(50) value of 34.95 μm. | 2012 | 22924533 |
| jasmonic acid is a crucial signal transducer in heat shock induced sesquiterpene formation in aquilaria sinensis. | agarwood, a highly valuable resinous and fragrant heartwood of aquilaria plants, is widely used in traditional medicines, incense and perfume. only when aquilaria trees are wounded by external stimuli do they form agarwood sesquiterpene defensive compounds. therefore, understanding the signaling pathway of wound-induced agarwood formation is important. jasmonic acid (ja) is a well-characterized molecule that mediates a plant's defense response and secondary metabolism. however, little is known a ... | 2016 | 26902148 |
| 2-(2-phenylethyl)chromone derivatives in artificial agarwood from aquilaria sinensis. | seven new 2-(2-phenylethyl)chromone derivatives (1-7) including a chlorinated one (4), together with eight known ones (8-15), were isolated from the etoac extract of artificial agarwood originating from aquilaria sinensis (lour.) gilg. all structures including the absolute configurations were unambiguously elucidated by spectroscopic (nmr, uv, ir, ms) methods, mosher's method, and comparison with reported data in the literatures. among those, compounds 8, 12, and 14 exhibited significant inhibit ... | 2016 | 26784520 |
| molecular cloning and characterization of three cdnas encoding 1-deoxy-d-xylulose-5-phosphate synthase in aquilaria sinensis (lour.) gilg. | agarwood is an expensive resinous heartwood derived from aquilaria plants that is widely used in traditional medicines, incense and perfume. the major constituents of agarwood oils are sesquiterpenes, which are obtained from isopentenyl diphosphate and dimethylallyl diphosphate precursors through the plastidial methylerythritol phosphate (mep) pathway and/or the cytosolic mevalonate pathway. 1-deoxy-d-xylulose-5-phosphate synthase (dxs) is the first rate-limiting enzyme for sesquiterpene synthes ... | 2014 | 24950429 |
| selection and validation of reference genes for studying stress-related agarwood formation of aquilaria sinensis. | agarwood is a high-valued woody material for medicine, perfume, and incense production in asia, middle east, and europe. the wild resources of agarwood-producing tree species, e.g., aquilaria sinensis have been greatly threatened. the formation of agarwood is considered to be associated with the plant stress and defensive responses, thus it would be urgent and significant to investigate the molecular mechanism of these species responding to a variety of stresses. this is the first report regardi ... | 2012 | 22678434 |
| new flavones, a 2-(2-phenylethyl)-4h-chromen-4-one derivative, and anti-inflammatory constituents from the stem barks of aquilaria sinensis. | in the current study, two new flavones, 4'-o-geranyltricin (1) and 3'-o-geranylpolloin (2), and a new 2-(2-phenylethyl)-4h-chromen-4-one derivative, 7-hydroxyl-6-methoxy-2-(2-phenylethyl)chromone (3), have been isolated from the stem barks of a. sinensis, together with 21 known compounds 4-24. the structures of new compounds 1-3 were determined through spectroscopic and ms analyses. compounds 2, 3, 5, 6, and 8-10 exhibited inhibition (ic50≤12.51 μm) of superoxide anion generation by human neutro ... | 2015 | 26610457 |
| effects of chinese herbal cataplasm xiaozhang tie on cirrhotic ascites. | conventional methods of treating cirrhotic ascites are inadequate. we sought to identify a novel, effective approach to relieve the suffering of patients with cirrhotic ascites. | 2011 | 22107835 |
| cloning, expression and characterization of coi1 gene (ascoi1) from aquilaria sinensis (lour.) gilg. | aquilaria sinensis, a kind of typically wounding-induced medicinal plant with a great economical value, is widely used in the production of traditional chinese medicine, perfume and incense. coronatine-insensitive protein 1 (coi1) acts as a receptor in jasmonate (ja) signaling pathway, and regulates the expression of ja-responsive genes in plant defense. however, little is known about the coi1 gene in a. sinensis. here, based on the transcriptome data, a full-length cdna sequence of coi1 (termed ... | 2015 | 26579478 |
| [liposolubility constituents from leaves of aquilaria sinensis]. | to study the chemical constituents of the leaves of aquilaria sinensis. | 2011 | 22066446 |
| bio-assay guided isolation and identification of α-glucosidase inhibitors from the leaves of aquilaria sinensis. | eight α-glucosidase inhibitors including four new compounds were isolated from the 70% aqueous ethanolic extract of leaves of aquilaria sinensis (lour.) gilg by activity-directed fractionation and purification processes. the ethanolic extract was first separated into petroleum ether, ethyl acetate, n-butanol and water soluble fractions and screened for inhibitory activity against α-glucosidase. further activity-directed investigation lead to the isolation of four new compounds with moderate inhi ... | 2011 | 21215978 |
| [simultaneous determination of benzophenones and xanthone in leaves of aquilaria sinensis by rp-hplc-uv]. | this study is to develop a sensitive method by using reversed-phase high performance liquid chromatography coupled with uv detector (hplc-uv) to simultaneously determine four bioactive compounds, iriflophenone 3-c-beta-d-glucoside, iriflophenone 3,5-c-beta-d-diglucoside, mangiferin, and iriflophenone 2-o-alpha-l-rhamnoside in the leaves of aquilaria sinensis. an agilent zorbax sb-c, column (4, 6 mm x 250 mm, 5 microm) was used, and the gradient elution was performed with mobile phase of 0.1% aqu ... | 2015 | 26281559 |
| molecular cloning, characterization and expression analysis of the gene encoding 1-deoxy-d-xylulose 5-phosphate reductoisomerase from aquilaria sinensis (lour.) gilg. | the major constituents of agarwood oils are sesquiterpenes that are obtained from isoprenoid precursors through the plastidial methylerythritol phosphate (mep) pathway and the cytosolic mevalonate pathway. in this study, a novel full-length cdna of 1-deoxy-d-xylulose 5-phosphate reductoisomerase (dxr), which was the second key enzyme in the plastid mep pathway of sesquiterpenes biosynthesis was isolated from the stem of aquilaria sinensis (lour.) gilg by the methods of reverse transcription poly ... | 2015 | 26174671 |
| [community structure and difference of endophtic bacteria in aquilaria sinensis with and without agarwood]. | aquilaria sinensis can generate agarwood, which is closely related with endophyte. up to now, studies mainly focused on the effects of endophytic fungi on agarwood formation, but studies about endophytic bacteria are rarely reported. in our research, the t-rfs and shannon index of endophytic bacteria in samples of agarwood increase. the number of distinctive t-rfs fragments of corresponding samples in the same group accounted for more than 60% the number of total t-rfs fragments. in samples of n ... | 2015 | 25993789 |
| the sesquiterpene biosynthesis and vessel-occlusion formation in stems of aquilaria sinensis (lour.) gilg trees induced by wounding treatments without variation of microbial communities. | as widely recognized, agarwood formation in aquilaria trees is induced by external wounding. because agarwood usually harbors specific microbes, the function of microbes in agarwood formation has been debated for almost a century. in this study, two wounding methods, the burning-chisel-drilling method (bcd) and the whole-tree agarwood-inducing method (agar-wit), were used under the non-contamination of environmental microorganisms. after pyrosequencing the small rrna subunits of the wounds induc ... | 2014 | 25530613 |
| [establishment of a cell suspension culture system of endangered aquilaria sinensis (lour.) gilg]. | aquilaria sinensis callus induced by stem tips were used to establish the suspension cell system. the results showed that the most suitable medium for callus induction and subculture is ms + 2.0 mg x l(-1) naa + 1.0 mg x l(-1) 6-ba. after 12 times of subculture, the energetic and loose callus, which were appropriate for cell suspension culture, were cultured and shook in liquid medium ms + 2.0 mg x l(-1) naa + 1.0 mg x l(-1) 6-ba + 500.0 mg x l(-1) casein hydrolysate (ch) to establish the suspen ... | 2014 | 25322564 |
| laxative effects of agarwood on low-fiber diet-induced constipation in rats. | agarwood (aquilaria sinensis), well known as incense in southeast asia, has been used as a digestive in traditional medicine. we investigated the laxative effects of an ethanol extract of agarwood leaves (eea) in a rat model of low-fiber diet-induced constipation. | 2010 | 21078136 |
| [cloning and expression analysis of cinnamate 4-hydroxylase (c4h) reductase gene from aquilaria sinensis]. | the study aimed to clone the open reading frame of cinnamate 4-hydroxylase (c4h) from aquilaria sinensis and analyze the bioinformatics and expression of the gene. one unique sequence containing c4h domain was discovered in our previous reported wound transcriptome dataset of a. sinensis. the open reading frame of c4h was cloned by rt-pcr strategy with the template of mixed rna extracted from a. sinensis stem which treated by different wound time. the bioinformatic analysis of this gene and its ... | 2014 | 25282879 |
| a new 2-(2-phenylethyl)chromone glycoside in chinese agarwood "qi-nan" from aquilaria sinensis. | a new 2-(2-phenylethyl)chromone glycoside, 2-[2-(4-glucosyloxy-3-methoxyphenyl)ethyl]chromone (1), was isolated from the high-quality chinese agarwood "qi-nan" originating from aquilaria sinensis (lour.) glig. the structure including the absolute configuration of the sugar moiety was elucidated by spectroscopic techniques (uv, ir, 1d and 2d nmr), ms analysis, pmp-labeling hplc analysis methods, as well as comparison with literature data. to the best of our knowledge, it is the first time that ch ... | 2017 | 27352768 |
| chromatographic fingerprint analysis of metabolites in natural and artificial agarwood using gas chromatography-mass spectrometry combined with chemometric methods. | agarwood is a resinous material formed in wounded aquilaria sinensis in china, which is widely used as an effective traditional chinese medicine (tcm). this study is aimed to use gas chromatography-mass spectrometry combined with chemometric methods to create reliable criteria for accurate identification of natural agarwood and artificial agarwood, as well as for quality evaluation of artificial agarwood. natural agarwood and artificial agarwood (stimulated by formic acid or formic acid plus fun ... | 2014 | 25129412 |
| five new 5,11-epoxyguaiane sesquiterpenes in agarwood "qi-nan" from aquilaria sinensis. | | 2016 | 27242216 |
| [cloning and gene expression of acetyl-coa c-acetyl transferase gene (asaact) from aquilaria sinensis]. | this study aimed to clone the acetyl-coa c-acetyl transferase (aact) gene from aquilaria sinensis and analyze the bioinformatics and expression of the gene. | 2014 | 24956835 |
| a new 2-(2-phenylethyl)chromone derivative in chinese agarwood 'qi-nan' from aquilaria sinensis. | phytochemical analysis of the high quality chinese agarwood 'qi-nan' originating from aquilaria sinensis (lour.) glig led to the isolation of a new 2-(2-phenylethyl)chromone derivative, qinanones g (1), and four known 2-(2-phenylethyl)chromones (2-5). their structures were elucidated by spectroscopic techniques (uv, ir, 1d and 2d nmr) and ms analyses. the nmr data of chromones 1-3 were first reported, and chromones 2 and 3 showed weak inhibitory activity against acetylcholinesterase. | 2014 | 24646200 |
| [cloning and expression analysis of hmg-coa reductase from aquilaria sinensis (lour.) gilg]. | 3-hydroxy-3-methylglutaryl-coenzyme a reductase (hmgr) is the first rate-limiting enzyme for sesquiterpene synthesis in the mevalonate (mva) pathway. the specific primers were designed according to the transcript sequence of ashmgr2 from the aquilaria sinensis (lour.) gilg transcriptome database. the full-length cdna of ashmgr2 was cloned by rt-pcr and rapid amplification of cdna ends (race) technology, and was analyzed at bioinformatics levels; ashmgr2 expression profiles in different tissues a ... | 2013 | 23984534 |
| 2-(2-phenylethyl)chromone derivatives in chinese agarwood "qi-nan" from aquilaria sinensis. | five new 2-(2-phenylethyl)chromone derivatives, qinanones a-e (1-5), together with eight known 2-(2-phenylethyl)chromone derivatives (6-13), were isolated from the et2o extract of high-quality chinese agarwood "qi-nan" originating from aquilaria sinensis. the structures of the new 2-(2-phenylethyl)chromones were elucidated by spectroscopic techniques (uv, ir, 1d and 2d nmr) and ms analyses. in the bioassay for acetylcholinesterase inhibitors, compounds 1-6, 10, and 12 exhibited weak inhibitory a ... | 2013 | 23929247 |
| [historical records and modern studies on agarwood production method and overall agarwood production method]. | agarwood is a precious traditional chinese medicine with the efficacy of promoting qi circulation and relieving pain, warming middle-jiao, controlling nausea and vomiting, governing inspiration and relieving asthma, therefore it is widely applied in the clinic. meanwhile, agarwood is also a precious spice. aquilaria sinensis is the only source of agarwood production in china. under natural conditions, a healthy a. sinensis tree produces no agarwood. only if being wounded or infected with fungus ... | 2013 | 23667998 |
| two new tirucallane triterpenoids from the leaves of aquilaria sinensis. | two new tirucallane triterpenoids, aquilacallanes a-b (1-2), together with 15 known compounds (3-17) were isolated from the leaves of aquilaria sinensis. the structures of these new compounds were elucidated on the basis of extensive spectroscopic analyses. all compounds were evaluated for their cytotoxic activity against five human cancer cell lines. the known compounds, ursolic acid (7) and 5,7,4'-trimethoxyflavone (14), exhibited weak cytotoxic activity against some cells. | 2013 | 23508744 |
| [the mechanical wound transcriptome of three-year-old aquilaria sinensis]. | chinese agarwood is formed in the aromatic resinous wood formed in aquilaria sinensis (lour.) gilg (botanical family: thymelaeaceae). only when suffering stress of wound, etc, can a. sinensis produce sesquiterpenes etc. compounds of agarwood around wounds. however, little is known about how wound induced the biosynthesis pathway of sesquiterpenes. to reveal the molecular mechanism of wound-induced agarwood formation, rna sequencing (rna-seq) technology was used to investigate the profile of gene ... | 2012 | 23162911 |
| quantification of polyphenols and pharmacological analysis of water and ethanol-based extracts of cultivated agarwood leaves. | mangiferin (3) and genkwanin 5-o-β-primeveroside (5) are the two major bioactive polyphenols with laxative property present in the extracts of agarwood (aquilaria sinensis) leaves (al). here we developed an hplc method to determine these bioactive components and four other major polyphenols in al extracts and evaluated the pharmacological equivalence of organic and water extracts. using mobile phase gradient conditions combined with uv detection at 330 nm, all six compounds were separated and we ... | 2012 | 22790572 |
| [constituents from the leaves of aquilaria sinensis]. | to study the chemical constituents of the leaves of aquilaria sinensis. | 2012 | 22737857 |
| synthesis and evaluation of benzophenone o-glycosides as α-glucosidase inhibitors. | the first total synthesis of benzophenone o-glycosides (iriflophenone 2-o-α-l-rhamnopyranoside: 1 and aquilarisinin: 2) isolated from the leaves of aquilaria sinensis and related new derivatives (3-12) was accomplished through suitable protecting group manipulations and glycosylation starting from commercially available l-rhamnose, d-glucose, d-galactose, d-mannose, d-xylose, and 1,3,5-trihydroxybenzene. all synthesized benzophenone o-glycosides were evaluated for their inhibitory activities aga ... | 2012 | 22730155 |
| identification of conserved and novel micrornas in aquilaria sinensis based on small rna sequencing and transcriptome sequence data. | agarwood is in great demand for its high value in medicine, incense, and perfume across asia, middle east, and europe. as agarwood is formed only when the aquilaria trees are wounded or infected by some microbes, overharvesting and habitat loss are threatening some populations of agarwood-producing species. aquilaria sinensis is such a significant economic tree species. to promote the production efficiency and protect the resource of a. sinensis, it would be critical to reveal the regulation mec ... | 2012 | 22521867 |
| [study on the physiological and biochemical characteristics of different types of leaves of aquilaria sinensis]. | to study the physiological and biochemical characteristics of different types of leaves of aquilaria sinensis. | 2011 | 22506384 |
| [chemical constituents of endophytic fungus nodulisporium sp. a4 from aquilaria sinensis]. | to investigate the chemical constituents of an endophytic fungus, nodulisporium sp. a4, from the medicinal plant aquilaria sinensis and search for antitumor natural products. | 2011 | 22393734 |
| aquilarin a, a new benzenoid derivative from the fresh stem of aquilaria sinensis. | chemical investigation of the etoh extract of the fresh stem of aquilaria sinensis collected in hainan province of china resulted in the isolation of a new benzenoid, named aquilarin a (1), together with two known compounds balanophonin (2) and (+)-lariciresinol (3). their structures were elucidated by a study of their physical and spectral data. compounds 2 and 3 exhibited cytotoxicity against sgc-7901 and smmc-7721 cell lines. | 2010 | 20657422 |
| two new 2-(2-phenylethyl)chromones from chinese eaglewood. | two new 2-(2-phenylethyl)chromones, (5s(*),6r(*),7s(*))-5,6,7-trihydroxy-2-(3-hydroxy-4-methoxyphenethyl)-5,6,7,8-tetrahydro-4h-chromen-4-one (1) and (5s(*),6r(*),7r(*))-5,6,7-trihydroxy-2-(3-hydroxy-4-methoxyphenethyl)-5,6,7,8-tetrahydro-4h-chromen-4-one (2), were isolated from the chinese eaglewood of aquilaria sinensis (lour.) gilg. their structures were established by detailed ms and nmr spectroscopic analysis, as well as comparison with the literature data. | 2010 | 20390756 |
| a new 2-(2-phenylethyl)chromone from chinese eaglewood. | a new 2-(2-phenylethyl)chromone, 5,6,7,8-tetrahydroxy-2-(3-hydroxy-4-methoxyphenethyl)-5,6,7,8-tetrahydro-4h-chromen-4-one (1) was isolated from the chinese eaglewood [aquilaria sinensis (lour.) gilg]. its structure was established by detailed ms and nmr spectroscopic analysis, as well as comparison with literature data. | 2009 | 20032882 |
| [studies on chemical constituents of leaves of aquilaria sinensis]. | to investigate the chemical constituents of the leaves of aquilaria sinensis, and provide a certain of basis for the comprehensive uses of the plant of a. sinensis. | 2009 | 19623981 |
| [study on karyotype and giemsa c-banding of aquilaria sinensis]. | to study aquilaria sinensis (lour.) spreng chromosome karyotype and c-banding. | 2007 | 17944179 |
| four new 2-(2-phenylethyl)chromone derivatives from withered wood of aquilaria sinensis. | four new chromone derivatives, 5-hydroxy-6-methoxy-2-(2-phenylethyl)chromone (1), 6-hydroxy-2-(2-hydroxy-2-phenylethyl)chromone (2), 8-chloro-2-(2-phenylethyl)-5,6,7-trihydroxy-5,6,7,8-tetrahydrochromone (3), 6,7-dihydroxy-2-(2-phenylethyl)-5,6,7,8-tetrahydrochromone (4) were isolated from the meoh extract of withered wood of aquilaria sinensis, together with seven known constituents of agarwood. | 2003 | 12736456 |
| [studies on the chemical constituents of aquilaria sinensis (lour.) gilg. v. isolation and characterization of three 2-(2-phenylethyl) chromone derivatives]. | three 2-(2-phenylethyl) chromone derivatives were isolated from the ethyl acetate soluble fraction of the alcoholic extract of aquilaria sinensis (lous.) gilg. (thymeleaceae) by silica gel chromatography. based on spectral data (uv, ir, 1hnmr, 13cnmr and ms) two of them are new compounds and their structures were identified as 5,8-dihydroxy-2-(2-p-methoxyphenylethyl) chromone (2) and 6,7-dimethoxy-2-(2-p-methoxyphenylethyl) chromone (3). the known compound siolated for the first time from this p ... | 1990 | 2101003 |
| [studies on the chemical constituents of aquilaria sinensis (lour) gilg. iii. elucidation of the structure of isobaimuxinol and isolation and identification of the constituents of lower boiling fraction of the volatile oil]. | a new sesquiterpenoid, named isobaimuxinol, c15h26o2, mp 73-75 degrees c, [a]d12-68(0) (c 0.10, chcl3), was isolated from the volatile oil of aquilaria sinensis (lour), gilg. (thymeleaceae). based on spectral (ir, 1h-nmr, 13c-nmr and 2d-nmr as well as ms) analysis its structure was identified as isobaimuxinol. the relative stereochemistry of isobaimuxinol was determined by x-ray crystallograhy. in addition, four known compounds, benzylacetone, p-methoxybenzylacetone, anisic acid and beta-agarofu ... | 1989 | 2816387 |
| [studies on the chemical constituents of aquilaria sinensis(lour.) gilg. iv. isolation and characterization of 2-(2-phenylethyl)chromone derivatives]. | six chromone derivatives were isolated from the ether soluble fraction of the alcoholic extract of aquilaria sinensis(lour.) gilg. (thymeleaceae) by silica gel chromatography. on the basis of spectrometric data (uv, ir, 1hnmr and 13cnmr as well as ms) and chemical synthesis one of them was found to be a new chromone, 6-hydroxy-2-[2-(4'-methoxylphenyl)ethyl]chromone(vi) and the other five known chromones are 2-(2-phenylethyl)chromone(i), 6-methoxy-2-(2-phenylethyl)chromone (ii), 6.7-dimethoxy-2-( ... | 1989 | 2618717 |
| [studies on the chemical constituents of aquilaria sinensis (lour.) gilg. ii. isolation and structure of baimuxinol and dehydrobaimuxinol]. | | 1986 | 3811941 |
| [effects of agarwood formation induced by fusarium sp. a2 on distribution and community composition of endophytic fungi in leaves of aquilaria sinensis]. | the aim of the paper is to observe the distribution of the endophytic fungi in leaves of aquilaria sinensis by using permanent paraffin-cut section, optical microscope photography and histochemistry. total dna was extracted with modified ctab method and rdna its regions of plant and endophytic fungi were amplified with eukaryotic universal primers. the rdna its amplicon was characterized by rflp analysis, sequencing of rdna its library and phylogenetic analyses using paup by maximum parsimony. f ... | 2014 | 24761631 |