| development of a rapid, sensitive and specific diagnostic assay for fish aquareovirus based on rt-pcr. | a rapid, sensitive and highly specific detection method for aquareovirus based on reverse-transcription polymerase chain reaction (rt-pcr) was developed. based on multiple sequence alignment of the cloned sequences of a local isolates, the threadfin reovirus (tfv) and guppy reovirus (gpv) with grass carp reovirus (gcrv), a pair of degenerate primers was selected carefully and synthesized. using this primer combination, only one specific product, approximately 450 bp in length was obtained when r ... | 2004 | 15081606 |
| molecular cloning, dna sequence analysis, and expression of cdna sequence of rna genomic segment 6 (s6) that encodes a viral outer capsid protein of threadfin aquareovirus (tfv). | the genome segment 6 (s6) of threadfin reovirus (tfv) was cloned and sequenced. the entire s6 nucleotide sequence is 2056 bp long with an open reading frame that encodes a protein of 653 amino acids. sequence analysis of the tfv s6 genome revealed that the 5'-terminal sequence, gtttta and the 3'-terminal sequence, attcatc of the plus strand is common to other genome segments of tfv. the pentanucleotide, tcatc, at the 3'-terminal of the plus strand was also conserved in other reported isolates of ... | 2005 | 15744578 |