| use of real-time pcr to detect and quantify mycoplasma haemofelis and "candidatus mycoplasma haemominutum" dna. | a real-time pcr assay using taqman probes was developed to detect and quantify mycoplasma haemofelis and "candidatus mycoplasma haemominutum" in feline blood samples. the assay was rapid and sensitive and was successfully used to monitor the in vivo kinetics of cats experimentally infected with each species. | 2003 | 12517888 |
| presence of mycoplasma haemofelis, mycoplasma haemominutum and piroplasmids in cats from southern europe: a molecular study. | clinical symptoms produced by mycoplasma spp. and piroplasmids in cats are sometimes similar. diagnosis of these pathogens is difficult by microscopic procedures and molecular methods have been used as an alternative. we present in this work, the development of new molecular procedures for diagnosis of the aforementioned organisms, together with a molecular characterization of isolates found in southern european cats.a single pcr-rflp procedure was designed for diagnosis of mycoplasma spp. and a ... | 2003 | 12713893 |
| infection with a proposed new subspecies of babesia canis, babesia canis subsp. presentii, in domestic cats. | parasitemia with a large babesia species was identified in two domestic cats from israel. one cat, also coinfected with feline immunodeficiency virus and "candidatus mycoplasma haemominutum," had profound icterus and anemia which resolved after therapy, whereas a second cat was an asymptomatic carrier. amplification and sequencing of the 18s rrna gene, followed by phylogenetic analyses, indicated that infection was caused by babesia canis. however, the sequences of the internal transcribed and 5 ... | 2004 | 14715738 |
| identification of a novel hemotropic mycoplasma in a splenectomized dog with hemic neoplasia. | a 3-year-old sexually intact male bull mastiff underwent splenectomy for splenic thrombosis; prior to and after splenectomy, multiple blood transfusions were administered. two weeks after the procedure, t-cell lymphoproliferative disease was diagnosed. treatment with prednisone and chlorambucil was initiated, and 2 weeks later, cytologic examination of a blood smear revealed small (0.3 microm), coccoid basophilic bodies on the surface of approximately 70% of the rbcs. morphologically, these rese ... | 2004 | 15230449 |
| anemia associated with 'candidatus mycoplasma haemominutum' in a feline leukemia virus-negative cat with lymphoma. | 'candidatus mycoplasma haemominutum,' previously known as the small form of haemobartonella felis (california species), is a hemotrophic parasite found on erythrocytes of infected cats. although fleas are potential vectors, confirmatory studies are lacking. healthy cats infected with 'candidatus mycoplasma haemominutum' generally do not have clinically significant anemia, but concurrent disease or immune suppression may predispose a cat to develop a life-threatening anemia, such as in the case r ... | 2004 | 15347624 |
| mycoplasma haemofelis and mycoplasma haemominutum detection by polymerase chain reaction in cats from saskatchewan and alberta. | hemobartonellosis is caused by mycoplasma haemofelis, previously known as haemobartonella felis. cats infected with this organism typically develop regenerative anemia. the related species mycoplasma haemominutum may also cause anemia. the purposes of this study were to use polymerase chain reaction technology to determine if both organisms exist in naturally infected cats from saskatchewan and alberta, and to determine if disease manifestation corresponds to mycoplasma species. thirteen of 18 c ... | 2004 | 15510683 |
| identification, molecular characterization, and experimental transmission of a new hemoplasma isolate from a cat with hemolytic anemia in switzerland. | recently, there has been a growing interest in hemotropic mycoplasmal species (also known as the hemoplasmas), the causative agents of infectious anemia in several mammalian species. in felids, two different hemoplasma species have been recognized: mycoplasma haemofelis (formerly haemobartonella felis) and "candidatus mycoplasma haemominutum." recently developed molecular methods have allowed sensitive and specific identification and quantification of these agents in feline blood samples. in app ... | 2005 | 15956367 |
| evaluation of experimental transmission of candidatus mycoplasma haemominutum and mycoplasma haemofelis by ctenocephalides felis to cats. | to determine whether ctenocephalides felis can transmit mycoplasma haemofelis (mhf) and candidatus mycoplasma haemominutum (mhm) through hematophagous activity between cats. | 2005 | 16008224 |
| attempted transmission of candidatus mycoplasma haemominutum and mycoplasma haemofelis by feeding cats infected ctenocephalides felis. | to determine whether mycoplasma haemofelis (mhf) and candidatus mycoplasma haemominutum (mhm) can be transmitted by ingestion of mycoplasma-infected ctenocephalides felis and by-products (feces, larvae, and eggs). | 2006 | 16506915 |
| prevalence, risk factor analysis, and follow-up of infections caused by three feline hemoplasma species in cats in switzerland. | recently, a third novel feline hemotropic mycoplasma sp. (aka hemoplasma), "candidatus mycoplasma turicensis," in a cat with hemolytic anemia has been described. this is the first study to investigate the prevalence, clinical manifestations, and risk factors for all three feline hemoplasma infections in a sample of 713 healthy and ill swiss cats using newly designed quantitative real-time pcr assays. "candidatus mycoplasma haemominutum" infection was detected in 7.0% and 8.7% and mycoplasma haem ... | 2006 | 16517884 |
| prevalence of dna of mycoplasma haemofelis, 'candidatus mycoplasma haemominutum,' anaplasma phagocytophilum, and species of bartonella, neorickettsia, and ehrlichia in cats used as blood donors in the united states. | to identify the prevalence of dna of mycoplasma haemofelis; 'candidatus mycoplasma haemominutum'; anaplasma phagocytophilum; and species of bartonella, neorickettsia, and ehrlichia in blood of cats used as blood donors in the united states. | 2006 | 16948578 |
| phylogenetic analysis of "candidatus mycoplasma turicensis" isolates from pet cats in the united kingdom, australia, and south africa, with analysis of risk factors for infection. | two hemotropic mycoplasmas have been recognized in cats, mycoplasma haemofelis and "candidatus mycoplasma haemominutum." we recently described a third feline hemoplasma species, designated "candidatus mycoplasma turicensis," in a swiss cat with hemolytic anemia. this isolate induced anemia after experimental transmission to two specific-pathogen-free cats and analysis of the 16s rrna gene revealed its close relationship to rodent hemotropic mycoplasmas. the agent was recently shown to be prevale ... | 2006 | 17035497 |
| worldwide occurrence of feline hemoplasma infections in wild felid species. | while hemoplasma infections in domestic cats are well studied, almost no information is available on their occurrence in wild felids. the aims of the present study were to investigate wild felid species as possible reservoirs of feline hemoplasmas and the molecular characterization of the hemoplasma isolates. blood samples from the following 257 wild felids were analyzed: 35 iberian lynxes from spain, 36 eurasian lynxes from switzerland, 31 european wildcats from france, 45 lions from tanzania, ... | 2007 | 17301277 |
| immune-mediated erythroid and megakaryocytic aplasia in a cat. | a 6-month-old domestic shorthair cat was evaluated because of acute lethargy. | 2007 | 17397342 |
| detection of mixed infections with "candidatus mycoplasma haemominutum" and mycoplasma haemofelis using real-time taqman polymerase chain reaction. | the objective of this study was to evaluate the use of real-time taqman pcr assays for detection of coinfections with "candidatus mycoplasma haemominutum" (mhm), and mycoplasma haemofelis (mhf), in vitro and over time in experimentally infected cats. first, the ability of each real-time pcr assay to detect and quantify mixed infections was determined in vitro by testing mixtures of plasmids containing mhm and mhf 16s rdna with each assay. subsequently, 4 specific pathogen-free (spf) cats, 2 of w ... | 2007 | 17459853 |
| real-time pcr investigation of potential vectors, reservoirs, and shedding patterns of feline hemotropic mycoplasmas. | three hemotropic mycoplasmas have been identified in pet cats: mycoplasma haemofelis, "candidatus mycoplasma haemominutum," and "candidatus mycoplasma turicensis." the way in which these agents are transmitted is largely unknown. thus, this study aimed to investigate fleas, ticks, and rodents as well as saliva and feces from infected cats for the presence of hemotropic mycoplasmas, to gain insight into potential transmission routes for these agents. dna was extracted from arthropods and from rod ... | 2007 | 17468284 |
| [detection frequency of haemoplasma infections of the domestic cat in germany]. | we present epidemiological data on the frequency of infections with haemotrophic mycoplasma spp. (feline haemoplasmas) in domestic cats in germany. from november 2004 to october 2006 135 blood samples of anaemic patients and cats without clinical symptoms were examined with conventional and real-time pcr methods. in 15,6 % of the samples dna of one or more haemoplasma species could be detected. 8,9 % of the samples (12 cats) were infected with "candidatus mycoplasma haemominutum, whereas 7,4 % ( ... | 2007 | 17555038 |
| molecular detection of "candidatus mycoplasma haemominutum" in a lion (panthera leo) from a brazilian zoological garden. | although mycoplasma haemofelis and "candidatus mycoplasma haemominutum" infections have been reported in wild cats from united states, their presence among native and captive wild cats in brazil is still unknown. a 12 year old healthy male lion (panthera leo) from the zoological garden of curitiba, brazil was anesthetized for transportation and dental evaluation. a blood sample was obtained for a complete blood cell count (cbc) and pcr analysis. dna was extracted and fragments of mycoplasma haem ... | 2007 | 17625699 |
| the prevalence of three species of feline haemoplasmas in samples submitted to a diagnostics service as determined by three novel real-time duplex pcr assays. | three distinct species of feline haemoplasmas are recognised: mycoplasma haemofelis (mhf), candidatus mycoplasma haemominutum (cmhm) and candidatus mycoplasma turicensis (cmt). these species differ in pathogenicity as mhf and cmt can be associated with anaemia whereas cmhm usually results in few clinical signs. the purpose of this study was to develop quantitative real-time pcr assays for the detection of all three feline haemoplasma species combined with an endogenous internal control and to de ... | 2008 | 17689890 |
| from haemobartonella to hemoplasma: molecular methods provide new insights. | hemotropic mycoplasmas (aka hemoplasmas) are the causative agents of infectious anemia in numerous mammalian species. originally known as haemobartonella and eperythrozoon species, these organisms have been reclassified within the genus mycoplasma. the development of new molecular assays has expanded our knowledge of this heterogeneous group of agents and allowed us to study their epidemiology and pathogenesis. the present review summarizes recently gained insights into feline hemotropic mycopla ... | 2007 | 17706380 |
| "candidatus mycoplasma haemominutum" infections in 21 client-owned cats. | medical records were reviewed for 21 clinically ill cats testing positive for deoxyribonucleic acid (dna) of "candidatus mycoplasma haemominutum" in their blood. fever, anorexia, lethargy, and anemia were among the most common abnormalities recorded. thirteen cats were anemic; seven had evidence of other diseases that could have been the primary cause of anemia or activated hemoplasmosis. for six cats, "candidatus mycoplasma haemominutum" was the only recognizable cause of the anemia. of these c ... | 2007 | 17823473 |
| prevalences of various hemoplasma species among cats in the united states with possible hemoplasmosis. | to determine prevalences of various hemoplasma species among cats in the united states with possible hemoplasmosis and identify risk factors for and clinicopathologic abnormalities associated with infection with each species. | 2008 | 18241101 |
| use of dried blood smears for detection of feline hemoplasmas using real-time polymerase chain reaction. | the objective of the current study was to determine the sensitivity and specificity of real-time polymerase chain reaction (real-time pcr) for feline hemoplasmas when applied to dna extracted from dried whole-blood smears in comparison to that for dna extracted from liquid whole blood. blood samples were collected into ethylenediamine tetra-acetic acid tubes from 305 cats with possible or suspected hemoplasmosis, and dried blood smears from each sample were prepared. dna was extracted from blood ... | 2008 | 18776095 |
| the occurrence of the feline "candidatus mycoplasma haemominutum" in dog in china confirmed by sequence-based analysis of ribosomal dna. | in the present study, polymerase chain reaction (pcr) assay was used to detect haemoplasmas (haemotropic bacteria) in 40 clinically healthy pet dogs in foshan city, guangdong province, china, and one dog was found positive. comparison of its 16s ribosomal dna (rdna) sequence with relevant sequences showed that the isolated haemoplasma had greater sequence identity to feline species "candidatus mycoplasma haemominutum" (99%) than to "candidatus mycoplasma haematoparvum" (95%). this result, for th ... | 2009 | 18949572 |
| the prevalence of bartonella, hemoplasma, and rickettsia felis infections in domestic cats and in cat fleas in ontario. | the prevalence of persistent bacteremic bartonella spp. and hemoplasma infections was determined in healthy pet cats in ontario. blood samples from healthy cats sent to a diagnostic laboratory for routine health assessment over the course of 1 y were tested for bartonella spp. using both polymerase chain reaction (pcr) and blood culture, and for the presence of hemoplasma by pcr. the overall prevalence of bartonella spp. by pcr and by culture combined was 4.3% (28/646) [3.7% (24/646) bartonella ... | 2008 | 19086373 |
| the application of pcr and reverse line blot hybridization to detect arthropod-borne hemopathogens of dogs and cats in trinidad. | arthropod-borne diseases are important causes of morbidity and mortality of companion animals in trinidad. as clinical signs are vague, more sensitive methods to diagnose these diseases based on the polymerase chain reaction (pcr) followed by reverse line blot hybridization (rlb) of amplified products are being developed. an rlb of 14 oligonucleotide probes coupled with polymerase chain-amplified regions of 16s rrna or 18s rrna genes of hemoparasites from cats and dogs detected ehrlichia canis, ... | 2008 | 19120209 |
| effects of imidocarb dipropionate in cats with chronic haemobartonellosis. | the objective of this study was to evaluate the safety and efficacy of imidocarb dipropionate administered to cats for clearing chronic haemobartonella felis infections. imidocarb dipropionate was administered twice at 5.0 mg/kg by intramuscular injection 14 days apart to eight cats with chronic, subclinical haemobartonellosis. clinical signs and laboratory parameters were monitored throughout the study. polymerase chain reaction (pcr) for the detection of mycoplasma haemofelis (large form of h. ... | 2002 | 19750745 |
| prevalence of feline haemoplasma infection in cats in southern bavaria, germany, and infection risk factor analysis. | in this prospective study performed from samples of 296 cats from southern bavaria, germany, a conventional pcr (polymerase chain reaction) assay for detection of mycoplasma haemofelis and "candidatus mycoplasma haemominutum" and a real-time pcr for "candidatus mycoplasma turicensis" were used to test blood samples from ill cats with anaemia (n = 79), ill cats with a normal haematocrit (n = 98), and healthy cats (n = 119). the aim of the study was to investigate the prevalence of feline haemopla ... | 2010 | 20135909 |
| feline hemotropic mycoplasmas. | to describe the current understanding of the etiology, pathogenesis, diagnosis, and treatment of feline hemotropic mycoplasmosis (feline infectious anemia). | 2010 | 20230435 |
| identification, characterization, and application of a recombinant antigen for the serological investigation of feline hemotropic mycoplasma infections. | in felids, three hemotropic mycoplasma species (hemoplasmas) have been described: mycoplasma haemofelis, "candidatus mycoplasma haemominutum," and "candidatus mycoplasma turicensis." in particular, m. haemofelis may cause severe, potentially life-threatening hemolytic anemia. no routine serological assays for feline hemoplasma infections are available. thus, the goal of our project was to identify and characterize an m. haemofelis antigen (dnak) that subsequently could be applied as a recombinan ... | 2010 | 20876820 |
| detection of humoral response using a recombinant heat shock protein 70, dnak, of mycoplasma haemofelis in experimentally and naturally hemoplasma-infected cats. | hemoplasmas is the trivial name given to a group of erythrocyte-parasitizing bacteria of the genus mycoplasma. of the feline hemoplasmas, mycoplasma haemofelis is the most pathogenic, while "candidatus mycoplasma haemominutum" and "candidatus mycoplasma turicensis" are less pathogenic. shotgun libraries of fragmented m. haemofelis genomic dna were constructed, and random colonies were selected for dna sequencing. in silico-translated amino acid sequences of putative open reading frames were comp ... | 2010 | 20926695 |
| feline hemotropic mycoplasmas. | three species of hemotropic mycoplasmas are known to infect cats worldwide, mycoplasma haemofelis, "candidatus mycoplasma turicensis" and "candidatus mycoplasma haemominutum." these organisms were previously known as haemobartonella felis, but are now known to be mycoplasmas. assays based on polymerase chain reaction technology are the most sensitive and specific diagnostic tests available for these organisms. m haemofelis is the most pathogenic species, and causes hemolytic anemia in immunocomp ... | 2010 | 20933142 |
| detection of feline haemoplasma species in experimental infections by in-situ hybridisation. | the aim of this study was to use fluorescence in-situ hybridisation (fish) to search for the tissues and cell types important in survival and persistence of mycoplasma haemofelis, "candidatus mycoplasma haemominutum" or "candidatus mycoplasma turicensis" in infected cats. a 16s rdna probe for each species was applied to formalin-fixed, paraffin wax-embedded tissues sections collected from experimentally infected cats. tissues (n = 12) were collected, at necropsy, from ten cats which had been inf ... | 2010 | 21129480 |
| feline leukemia virus outbreak in the critically endangered iberian lynx (lynx pardinus): high-throughput sequencing of envelope variable region a and experimental transmission. | the iberian lynx is the most endangered felid species. during winter/spring 2006/7, a feline leukemia virus (felv) outbreak of unexpected virulence killed about 2/3 of the infected iberian lynxes. all felv-positive animals were co-infected with feline hemoplasmas. to further characterize the iberian lynx felv strain and evaluate its potential virulence, the felv envelope gene variable region a (vra) mutant spectrum was analyzed using the roche 454 sequencing technology, and an in vivo transmissi ... | 2011 | 21302124 |
| development of multiplex polymerase chain reaction for detection of feline hemotropic mycoplasma in blood and tissue specimens. | a multiplex polymerase chain reaction (pcr) was developed for the detection of feline hemotropic mycoplasmas which simultaneously differentiates infections of mycoplasma haemofelis (mhf), candidatus mycoplasma haemominutum (cmhm) and candidatus mycoplasma turicensis (cmtc) in feline blood and spleen. these organisms are responsible for the cause of various pathogenicity of feline infectious anemia. these infections are difficult to be detected by microscopic examination, the most commonly used m ... | 2010 | 21329322 |
| genome sequence for "candidatus mycoplasma haemominutum," a low-pathogenicity hemoplasma species. | we present the genome sequence of "candidatus mycoplasma haemominutum" strain birmingham 1, a low-pathogenicity feline hemoplasma strain. | 2012 | 22275103 |
| infectious disease prevalence in a feral cat population on prince edward island, canada. | ninety-six feral cats from prince edward island were used to determine the prevalence of selected infectious agents. the prevalence rates were 5.2% for feline immunodeficiency virus, 3.1% for feline leukemia virus, 3.1% for mycoplasma haemofelis, 8.4% for candidatus mycoplasma haemominutum, 2.1% for bartonella spp. and 29.8% for exposure to toxoplasma gondii. oocysts of t. gondii were detected in 1.3% of the fecal samples that were collected. gender and retroviral status of the cats were signifi ... | 2011 | 22379197 |
| hemoplasmas in wild canids and felids in brazil. | hemotropic mycoplasmas, epicellular erythrocytic bacterial parasites lacking a cell wall, are the causative agents of infectious anemia in numerous mammalian species. the presence of hemotropic mycoplasmas in blood samples of neotropical and exotic wild canids and felids from brazilian zoos were recorded using molecular techniques. blood samples were collected from 146 brazilian wild felids, 19 exotic felids, 3 european wolves (canis lupus), and from 97 brazilian wild canids from zoos in the bra ... | 2011 | 22946419 |
| frequency of piroplasms babesia microti and cytauxzoon felis in stray cats from northern italy. | emerging diseases caused by piroplasms pose a health risk for man and other animals, and domestic cats have been proposed as potential reservoirs for some piroplasm infections. the aim of this study was to identify the frequency of the piroplasms babesia microti and cytauxzoon felis in stray cats from northern italy and to identify possible risk factors associated with these infections. blood samples from 260 stray cats enrolled in a trap-neuter-release (tnr) program in northern italy were exami ... | 2014 | 24895629 |
| survey of toxoplasma gondii and neospora caninum, haemotropic mycoplasmas and other arthropod-borne pathogens in cats from albania. | albania is a country on the western part of the balkan peninsula. the mediterranean climate is favourable for the stable development of many arthropod species, which are incriminated as vectors for various agents. recently, several papers have reported on epidemiological aspects of parasitic diseases including vector-borne disease agents of dogs with zoonotic characteristics in albania. however, data on the epidemiology of feline parasitic and bacterial agents in albania is scarce. | 2014 | 24517118 |
| arthropod-borne pathogens circulating in free-roaming domestic cats in a zoo environment in brazil. | recently, tick and flea-borne pathogens have been detected in wild carnivores maintained in captivity in brazilian zoos. since free-roaming cats are frequently found in brazilian zoos, they could act as reservoirs for arthropod-borne pathogens, which could be transmitted to endangered wild carnivores maintained in captivity in these institutions. on the other hand, stray cats in zoos may play a role as sentinels to pathogens that circulate among wild animals in captivity. the present work aimed ... | 2014 | 24889035 |
| prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from cyprus. | feline infectious agent studies are lacking in cyprus. the aims of this study were to determine the prevalence and risk factors for various feline infectious agents, including feline vector-borne pathogens (fvbp), in cats from cyprus. | 2017 | 28285597 |
| suspected myelodysplastic/myeloproliferative neoplasm in a feline leukemia virus-negative cat. | a 10-year-old castrated domestic short-haired cat was presented to a primary care veterinarian for a wellness examination and laboratory examination for monitoring of diabetes mellitus. the cbc revealed marked thrombocytosis, leukopenia and macrocytic, normochromic anemia. the cat tested negative for felv and feline immunodeficiency virus, but was positive for mycoplasma haemominutum by pcr. hematologic abnormalities were not responsive to therapy, so a repeat cbc and a bone marrow aspiration fo ... | 2016 | 27870069 |
| prevalence and molecular characterization of canine and feline hemotropic mycoplasmas (hemoplasmas) in northern italy. | hemotropic mycoplasmas (hemoplasmas), the agents of infectious anemia, have been reported in dogs and cats. little data are available on hemoplasma infections in italy. the aim of this study was to evaluate the species of hemoplasmas and their prevalence in dogs and cats of northern italy. | 2017 | 28285588 |
| identification, occurrence and clinical findings of canine hemoplasmas in southern brazil. | hemoplasmas are ubiquitous pleomorphic and epicellular bacteria detected in erythrocytes in several species. in brazil, studies on hemoplasmas have not included information on occurrence, clinical signs, and risk factors in dogs. this paper investigates the occurrence of hemoplasmas in dogs, focusing on risk factors and clinical status. conventional pcr for the four types of canine hemoplasmas was performed in 331 blood samples collected from dogs clinically treated at a teaching veterinary hosp ... | 2014 | 25159996 |
| prevalence of feline haemoplasma in cats in denmark. | infections with the three feline haemotropic mycoplasmas mycoplasma haemofelis, candidatus mycoplasma haemominutum and candidatus mycoplasma turicensis cause feline infectious anemia. the purpose of this study was to investigate the prevalence of carriage of feline haemoplasma in danish cats in different age groups. the presence was detected by a conventional polymerase chain reaction (pcr) assay on blood samples as well as by real-time pcr (rt-pcr). | 2016 | 27832803 |
| molecular detection and prevalence of feline hemotropic mycoplasmas in istanbul, turkey. | the aim of this study was to investigate mycoplasma spp. species in blood samples of the domestic cats from the province of istanbul, turkey. three hundred eighty four blood samples of client-owned cats were used for the identification of mycoplasma haemofelis (mhf), candidatus mycoplasma haemominutum (cmhm) and candidatus mycoplasma turicensis (cmt) by polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp) assays. out of 384 blood samples, 74 (19.3%) were positive f ... | 2016 | 26751888 |
| a molecular study of hemotropic mycoplasmas (hemoplasmas) in cats in iran. | three feline hemoplasma species are recognized: mycoplasma haemofelis, 'candidatus mycoplasma haemominutum', and 'candidatus mycoplasma turicensis'. these species can cause anemia in cats and have a worldwide distribution. | 2014 | 24948469 |
| prevalence of haemoplasma infections in stray cats in northern italy. | this study investigated the prevalence of feline haemoplasma infections in a number of stray cat colonies in milan, northern italy. blood samples from 260 stray cats were evaluated, with conventional pcr, for the presence of dna associated with mycoplasma haemofelis (mhf) and "candidatus mycoplasma haemominutum" (cmhm). odd ratios (or) were calculated to identify risk factors for haemoplasma infections. pcr was positive in 86 out of 260 subjects (33.1%), with a prevalence of 10.8% (28/260 cats) ... | 2014 | 24707436 |
| rapid detection of haemotropic mycoplasma infection of feline erythrocytes using a novel flow cytometric approach. | the haemotropic mycoplasmas mycoplasma haemofelis and candidatus mycoplasma haemominutum cause feline infectious anaemia with infection rates in feline populations reflecting widespread subclinical infection. clinically significant infections are much rarer but can be life-threatening. current diagnosis is dependent upon visualising organisms in stained blood smears, pcr or quantitative pcr (qpcr). these procedures are labour-intensive and time-consuming. furthermore, pcr-based approaches offer ... | 2013 | 23725366 |
| prevalence and co-infection of haemotropic mycoplasmas in portuguese cats by real-time polymerase chain reaction. | the diagnosis of feline haemoplasmosis has improved over the years, with several techniques enabling a clear and specific diagnosis, and where polymerase chain reaction (pcr) is considered as the 'gold standard'. the aim of this study was to survey the prevalence of feline haemoplasmas in 320 cats from the north-central region of portugal by the use of real-time pcr, as well as to evaluate any associations between infection, clinical presentation and risk factors. the overall prevalence of infec ... | 2013 | 23482254 |
| molecular detection of hemoplasma infection among cats from são luís island, maranhão, brazil. | hemoplasmas are bacteria that infect erythrocytes, attaching to the red blood cell. there is a need for more reports of hemoplasma infection prevalence and molecular characterization among cats in brazil since there are only few published reports. the present work aimed to detect and molecularly characterize the presence of hemotrophic mycoplasmas in domestic cats with outdoor access from são luís, maranhão, brazil. twenty cats (10%) were positive for candidatus m. haemominutum, five (2.5%) for ... | 2012 | 24031865 |
| molecular detection of vector-borne pathogens in dogs and cats from qatar. | vector-borne diseases (vbds) have been increasingly reported in dogs and cats worldwide. however, no data are currently available regarding canine and feline vbds in qatar and limited information is available from other persian gulf countries. | 2017 | 28633666 |
| molecular detection of vector-borne pathogens in greek cats. | infectious diseases have been increasingly recognized in cats worldwide. the objective of this study was the molecular investigation of the prevalence of selected pathogens in healthy and sick cats from greece, a country highly endemic for several canine vector-borne pathogens. blood and/or bone marrow samples from 50 clinically healthy and 50 sick adult (>1 year-old) cats were retrospectively examined for the amplification of bartonella spp., haemoplasmas, ehrlichia spp., anaplasma spp., babesi ... | 2017 | 28887102 |